trigram

This is a table of type trigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

trigram	frequency
mediated isothermal amplification	750
the detection of	234
rapid detection of	207
for the detection	187
for detection of	149
reverse transcription loop	140
in this study	133
of the rt	132
for rapid detection	119
the use of	111
polymerase chain reaction	109
as well as	100
nucleic acid amplification	96
the sensitivity of	93
severe acute respiratory	91
acute respiratory syndrome	89
a license to	88
to display the	88
display the preprint	88
who has granted	88
license to display	88
has granted medrxiv	88
granted medrxiv a	88
medrxiv a license	88
the preprint in	88
the copyright holder	86
the lamp reaction	85
holder for this	85
copyright holder for	85
the presence of	85
isothermal amplification assay	83
of the lamp	83
detection of sars	82
according to the	82
isothermal amplification method	82
the lamp assay	82
the author funder	78
is the author	78
lamp assay was	77
this version posted	77
isothermal amplification for	75
which was not	75
f and b	75
respiratory syndrome coronavirus	75
based on the	72
sensitivity of the	70
sensitivity and specificity	70
detection limit of	69
amplification of dna	66
by peer review	65
was not certified	65
certified by peer	65
not certified by	65
in order to	64
the specificity of	63
isothermal amplification of	60
sybr green i	60
lamp assay for	59
can be used	58
c for min	58
as shown in	57
loopmediated isothermal amplification	55
the development of	55
detection of the	55
and evaluation of	55
assay for the	55
development of a	55
specificity of the	55
notomi et al	54
ibv and ndv	53
loop mediated isothermal	53
due to the	53
agarose gel electrophoresis	52
the results of	52
time reverse transcription	51
preprint in perpetuity	50
rapid diagnosis of	50
development and evaluation	50
amplification assay for	49
the detection limit	48
the n gene	48
visual detection of	48
the lamp method	48
evaluation of a	47
for this preprint	47
used for the	47
the need for	46
the diagnosis of	45
a total of	45
found to be	45
amplification method for	45
under isothermal conditions	44
to determine the	44
limit of detection	44
was found to	43
of the target	42
a set of	42
a reverse transcription	42
by reverse transcription	42
the number of	41
was carried out	41
method for rapid	41
fip and bip	40
new england biolabs	40
of novel coronavirus	40
primers were designed	40
one of the	40
this study was	39
preprint this version	39
this preprint this	39
of autophagy in	39
was used as	39
of a novel	39
this this version	38
for this this	38
rapid detection and	38
the reaction mixture	37
of severe acute	37
specificity and sensitivity	37
been shown to	37
were used to	37
bst dna polymerase	37
that the rt	36
in the reaction	36
nucleic acid sequence	36
of the reaction	36
used in this	36
rapid and sensitive	36
nucleic acid testing	36
lamp assay and	36
the performance of	35
assay for detection	35
that of the	35
positive and negative	35
was added to	35
for the diagnosis	34
have been developed	34
for the rapid	34
shown in table	34
added to the	34
used in the	34
detection of mycobacterium	33
of reverse transcription	33
detection of human	33
amplification for rapid	33
parida et al	33
lamp assay is	33
in the presence	32
shown in fig	32
of mycobacterium tuberculosis	32
japanese encephalitis virus	31
of nucleic acid	31
was performed using	31
has been shown	31
serial dilutions of	31
a and b	31
of nucleic acids	31
the present study	31
the reaction was	31
used to detect	31
for diagnosis of	30
the target dna	30
west nile virus	30
of japanese encephalitis	30
in the field	30
assay for rapid	30
isothermal amplification reaction	30
used as a	30
all rights reserved	30
method for the	29
more sensitive than	29
reuse allowed without	29
for min and	29
no reuse allowed	29
and specificity of	29
allowed without permission	29
be used for	29
was used to	29
were collected from	29
the naked eye	28
the limit of	28
is made available	28
can be detected	28
to the reaction	28
rna was extracted	28
detection and differentiation	28
international license it	28
be used to	28
in the lamp	28
for the rt	28
available under a	28
lamp and rt	28
has been used	28
recombinase polymerase amplification	28
license it is	28
it is made	28
magnetic bead rna	28
was extracted from	28
made available under	28
and differentiation of	28
because of the	27
version posted november	27
a rapid and	27
limit of the	27
sensitive detection of	27
role of autophagy	27
zhang et al	27
preprint the copyright	27
detection of viral	27
of infectious diseases	26
isothermal nucleic acid	26
than that of	26
to evaluate the	26
a detection limit	26
at room temperature	26
results of the	26
influenza a viruses	26
nucleic acid detection	26
at c for	26
of influenza a	26
hepatitis c virus	26
feline infectious peritonitis	26
in the rt	26
was used for	26
the rapid detection	26
of clinical samples	26
the spread of	26
detection of novel	26
compared to the	26
j o u	25
o o f	25
in addition to	25
of the virus	25
lamp reaction was	25
of the assay	25
p r o	25
shown in figure	25
p r e	25
using loop primers	25
lamp products were	25
n a l	25
r o o	25
u r n	25
o u r	25
and can be	25
to detect the	25
of dengue virus	25
the target sequence	25
r n a	25
a l p	24
for min at	24
l p r	24
a novel loop	24
showed that the	24
it has been	24
mori et al	24
high sensitivity and	24
has also been	24
in clinical samples	24
can be performed	24
performance of the	24
direct detection of	24
for rapid diagnosis	24
isothermal amplification using	24
east respiratory syndrome	23
accelerated reaction by	23
to that of	23
samples with a	23
the formation of	23
a water bath	23
a constant temperature	23
isothermal amplifi cation	23
can also be	23
middle east respiratory	23
were used as	23
time detection of	23
detection of severe	23
porcine circovirus type	23
of zika virus	23
was performed in	23
the e gene	23
is based on	23
a number of	23
in a single	23
were obtained from	23
we developed a	23
of the samples	23
samples were collected	22
from patients with	22
of the primers	22
carried out in	22
a variety of	22
and quantification of	22
bead rna extraction	22
performed in a	22
does not require	22
and rapid detection	22
to develop a	22
for nucleic acid	22
a simple and	22
the role of	22
amplification and detection	22
herpes simplex virus	22
rapid and real	22
mediated isothermal amplifi	22
for min in	22
simple and rapid	22
that can be	21
version posted july	21
eiken chemical co	21
end of the	21
detection of porcine	21
clinical diagnosis of	21
armored rna particles	21
approved by the	21
amplification using loop	21
pdm h n	21
and sensitive detection	21
copies of the	21
lamp and pcr	21
lamp has been	21
by using the	21
has been reported	21
the optimal reaction	21
were carried out	21
data not shown	20
able to detect	20
compared with the	20
the risk of	20
are shown in	20
in terms of	20
avian influenza virus	20
of a loop	20
results showed that	20
fold serial dilutions	20
detection and quantification	20
newcastle disease virus	20
of viral rna	20
was shown to	20
due to its	20
and detection of	20
were used for	20
without the need	20
of the disease	20
of sybr green	20
there was no	20
such as the	20
on the other	20
sensitivity of rt	20
a dna polymerase	19
reaction by loop	19
a agarose gel	19
lamp can be	19
min in a	19
followed by cycles	19
the assay was	19
of west nile	19
lamp was performed	19
from magnesium pyrophosphate	19
was approved by	19
from clinical samples	19
pmol each of	19
for rapid and	19
could be used	19
h n and	19
copies per reaction	19
water bath or	19
method for detection	19
sensitive and specific	19
developed in this	19
there is no	19
the lamp primers	19
the other hand	19
to detect sars	19
in the present	19
hybridization chain reaction	18
the reaction tube	18
at a constant	18
the united states	18
lamp primers were	18
assay was developed	18
by cycles of	18
a sensitivity of	18
strand displacement activity	18
of this assay	18
detection of west	18
autophagy and apoptosis	18
reaction was performed	18
linked immunosorbent assay	18
the sensitivity and	18
the sequences of	18
indicating that the	18
by a loop	18
the amplified products	18
reaction can be	18
virus infection by	18
turbidity derived from	18
the mixture was	18
lamp is a	18
version posted may	18
the results were	18
to be a	18
were subjected to	18
in patients with	18
were positive for	18
with the rt	18
nagamine et al	18
primers targeting the	18
the addition of	18
of the sars	18
on the target	18
point of care	18
isothermal dna amplification	18
rna extracted from	17
be used as	17
has been developed	17
study was approved	17
forward inner primer	17
magnesium pyrophosphate formation	17
the authors declare	17
reaction by turbidity	17
in less than	17
igm and igg	17
dengue virus serotypes	17
false positive results	17
as a rapid	17
assay can be	17
a is the	17
derived from magnesium	17
respiratory syndrome virus	17
by turbidity derived	17
autophagy in the	17
the study was	17
viral nucleic acids	17
lamp assay can	17
sequence of the	17
of herpes simplex	17
hepatitis b virus	17
under a is	17
for the lamp	17
is one of	17
lamp assay has	17
of this study	17
indicated that the	17
by using a	17
of the rna	17
the nucleic acid	17
detection of influenza	17
and stored at	17
for infectious diseases	17
of viral nucleic	17
in sputum samples	17
for use in	17
the qlamp assay	17
been used for	17
with that of	17
set of four	17
displacement dna synthesis	17
real time rt	17
this work was	17
orf a and	17
molecular detection of	17
ranging from to	17
is shown in	17
isothermal amplification rapid	16
samples that were	16
the results showed	16
rapid and simple	16
a color change	16
pcr and real	16
of a reverse	16
lamp assay with	16
sensitive and rapid	16
amplification reaction by	16
in the same	16
of the most	16
the samples were	16
samples collected from	16
were performed in	16
higher than that	16
the je virus	16
in situ rt	16
pcr and rt	16
all of the	16
as a template	16
have also been	16
time monitoring of	16
the absence of	16
for detecting sars	16
for detection and	16
regions of the	16
lamp primer sets	16
the viral rna	16
amplification for the	16
of lamp is	16
respiratory syncytial virus	16
using clinical samples	16
be detected by	16
diagnostic method for	16
c for s	16
influenza a virus	16
backward inner primer	16
of detection of	16
by agarose gel	16
isothermal amplification and	16
color change from	16
by notomi et	16
the orf ab	16
mycobacterium tuberculosis complex	15
infectious bronchitis virus	15
can be found	15
were incubated at	15
in our study	15
the amplification reaction	15
detection method for	15
used as the	15
pcr and lamp	15
isothermal amplification assays	15
temperature for min	15
region of the	15
been used to	15
specific detection of	15
cyp c g	15
using the primer	15
the microfluidic disc	15
and n gene	15
the reaction is	15
the newly developed	15
evaluation of the	15
the treatment of	15
thiessen et al	15
has been widely	15
differentiation of dengue	15
has been applied	15
carried out using	15
of chikungunya virus	15
and the results	15
of f and	15
in a total	15
out in a	15
in response to	15
with high sensitivity	15
lamp assays were	15
in the detection	15
most of the	15
sequences of the	15
human immunodeficiency virus	15
demonstrated that the	15
of lamp reaction	15
world health organization	15
lf and lb	15
the majority of	15
chain reaction for	15
of the assays	15
multisystemic wasting syndrome	15
orf ab gene	15
the amplification of	15
of the ns	14
based on a	14
with high specificity	14
the detection and	14
primer explorer v	14
rolling circle amplification	14
with ethidium bromide	14
dilutions of the	14
the principle of	14
the causative agent	14
detection rate of	14
of four primers	14
the reaction time	14
postweaning multisystemic wasting	14
thermo fisher scientific	14
was supported by	14
nucleic acid extraction	14
dna polymerase with	14
in the acute	14
combined with a	14
as a result	14
the most common	14
in the future	14
lamp and the	14
be due to	14
amplification of the	14
study was to	14
a novel coronavirus	14
with a detection	14
large numbers of	14
to increase the	14
a primer set	14
with a ct	14
the negative control	14
of chik virus	14
complementary to the	14
and sensitivity of	14
on a agarose	14
because of its	14
there is a	14
t rna polymerase	14
using reverse transcription	14
of the primer	14
the fluorescence signal	14
two loop primers	14
the ability to	14
of the ap	14
strand displacement dna	14
regions on the	14
in the study	14
the concentration of	14
that the lamp	14
high specificity and	14
have been reported	14
based detection of	14
integrated isothermal device	14
isothermal amplification techniques	14
viral rna was	14
the conventional lamp	14
results indicated that	14
the concentrations of	14
shows that the	14
it can be	14
with those of	14
assay and the	14
a mixture of	14
detection of loop	14
a positive result	14
the detection rate	14
of the two	14
assay to detect	13
to assess the	13
to confirm the	13
infection by a	13
sequences in the	13
molecular diagnosis of	13
detection of japanese	13
involved in the	13
were analyzed by	13
used as templates	13
of the novel	13
used to determine	13
of a rapid	13
pcr products were	13
of the sample	13
of mycobacterium ulcerans	13
primer sets were	13
virus by reverse	13
transcriptase polymerase chain	13
of lamp for	13
of the f	13
a standard curve	13
pcr was performed	13
conflict of interest	13
were detected by	13
influenza a and	13
by the rt	13
analysis of the	13
and negative samples	13
chen et al	13
and application of	13
and respiratory syndrome	13
the advantages of	13
in the case	13
simple visual detection	13
in a clinical	13
of each primer	13
the onset of	13
samples were positive	13
each of the	13
the effect of	13
detection of herpes	13
the qpcr assay	13
of porcine circovirus	13
the opiph system	13
was determined by	13
of porcine parvovirus	13
no conflict of	13
for direct detection	13
carried out at	13
of dna amplification	13
under uv light	13
could be detected	13
were compared with	13
included in the	13
intracellulare in sputum	13
to be the	13
lamp reactions were	13
detection of infectious	13
and the reaction	13
of the amplification	13
for min to	13
amplification detection of	13
was performed as	13
the production of	13
hbv lamp reaction	13
two outer primers	13
gel electrophoresis and	13
the primer set	13
of the viral	13
the acute phase	13
with or without	13
amplification for direct	13
genbank accession number	13
for s and	13
in accordance with	13
by the naked	13
intravenous alteplase infusion	13
the amplifi cation	13
detection of zika	13
rna extraction kit	12
were used in	12
which can be	12
clinical samples were	12
reverse transcription polymerase	12
were designed to	12
h n virus	12
transcription polymerase chain	12
in the united	12
polymerase with strand	12
use of the	12
present in the	12
were able to	12
mthfr c t	12
causative agent of	12
comparison of the	12
shown to be	12
it is not	12
and simple visual	12
the base apparatus	12
that the detection	12
assay for detecting	12
samples were tested	12
need for a	12
is involved in	12
in this review	12
were designed using	12
pcr assay for	12
targeting the orf	12
transcription loop mediated	12
as described in	12
as described previously	12
reproductive and respiratory	12
of the positive	12
collected from patients	12
products were detected	12
between autophagy and	12
of autophagy and	12
this study is	12
the end of	12
dried blood spots	12
laboratory diagnosis of	12
acute phase of	12
was able to	12
the basis of	12
molecular detection mix	12
products of the	12
the accuracy of	12
dna or rna	12
detection of covid	12
the combination of	12
and b primers	12
was performed at	12
evaluation of reverse	12
of lamp primers	12
targeting the n	12
the replication of	12
tool for the	12
viral hemorrhagic septicaemia	12
the accumulation of	12
and visual detection	12
dna and rna	12
the process of	12
lamp assay using	12
use of a	12
optimal reaction temperature	12
there are several	12
of a single	12
well as the	12
lamp primers targeting	12
diagnosis of infectious	12
porcine reproductive and	12
been developed for	12
the form of	12
with a high	12
performed using the	12
positive in the	12
on the basis	12
mixture was incubated	12
the polymerase chain	12
the case of	12
of bst dna	12
viral rna in	12
rna extraction protocol	12
the orf a	12
qpcr and rt	12
of primers and	12
colorimetric detection of	12
the magnetic bead	12
of hepatitis c	12
is a novel	12
lamp assay were	12
fish and shellfish	12
distinct regions on	12
of target rna	12
was incubated at	12
diagnosis of fip	12
was performed by	11
of human herpesvirus	11
authors declare no	11
the reaction mix	11
electrophoresis on a	11
provided by the	11
cov and mers	11
members of the	11
in real time	11
for the development	11
of six primers	11
detection limits of	11
lamp primer set	11
and urine samples	11
without rna extraction	11
were designed by	11
dna was extracted	11
results in the	11
as part of	11
g for min	11
o c for	11
set of primers	11
can be observed	11
poon et al	11
nucleic acids in	11
supplementary table s	11
assays have been	11
real time pcr	11
of clinical specimens	11
loop primers loop	11
be used in	11
f fimbriae gene	11
of middle east	11
less than min	11
of hpv dna	11
of loop mediated	11
and h n	11
in agreement with	11
rapid identification of	11
to compare the	11
used as template	11
specificity of rt	11
total volume of	11
virus serotypes by	11
and virus isolation	11
health and disease	11
viral nucleic acid	11
the primers were	11
copy number of	11
has not been	11
listed in table	11
stained with ethidium	11
of human papillomavirus	11
detection of middle	11
acid amplification tests	11
with strand displacement	11
sequences and simple	11
incubated for min	11
copy numbers of	11
for rna extraction	11
the reactions were	11
strand displacement amplification	11
compared with those	11
by a real	11
were stored at	11
for the clinical	11
monitoring of the	11
were found to	11
less sensitive than	11
is needed to	11
serotype specific rt	11
the release of	11
method to detect	11
followed by a	11
as a positive	11
of loop primers	11
ns serotype specific	11
the coronavirus disease	11
in this work	11
the primer explorer	11
as a diagnostic	11
for the first	11
is the first	11
the lowest detection	11
in a water	11
preprint in the	11
the high sensitivity	11
using a reverse	11
in contrast to	11
transmissible gastroenteritis coronavirus	11
obtained from the	11
the specificity and	11
been developed to	11
acid amplification method	11
of patients with	11
detect viral rna	11
been applied to	11
was used in	11
conserved region of	11
a positive control	11
for disease control	11
results can be	11
dna amplification method	11
lamp assay developed	11
of a new	11
amplified products were	11
have to be	11
isothermal amplification a	11
lamp assay showed	11
reaction time of	11
rapid and reliable	11
the same as	11
with and without	11
c g g	11
to detect viral	11
the rna extraction	11
reaction for the	11
the novel rt	11
the identification of	11
for influenza a	11
version posted august	11
the amount of	11
the design of	11
to test the	11
in the copyright	11
reverse transcription and	11
method has been	11
influenza virus infection	11
an increase in	11
new generation of	11
the target gene	11
diagnosis of h	11
min and then	11
a new generation	11
n gene of	11
such as a	11
a range of	11
in developing countries	11
dna extraction and	11
when the template	10
supported by the	10
the intensity of	10
to achieve a	10
amplification for detection	10
to the results	10
in case of	10
detection of nucleic	10
time pcr for	10
use of this	10
sensitivity of lamp	10
been found to	10
was to develop	10
rapid molecular detection	10
to overcome the	10
the standard curve	10
prevention and control	10
samples from patients	10
the colorimetric rt	10
rapid and specific	10
detection of pathogens	10
results indicate that	10
lamp assay in	10
found in the	10
a panel of	10
in the clinical	10
the novel coronavirus	10
due to their	10
was detected in	10
isothermal master mix	10
of dna loop	10
classical swine fever	10
performed using a	10
of isothermal amplification	10
dried blood spot	10
this method has	10
virol methods doi	10
viral load in	10
been used in	10
application of the	10
to be used	10
gene sequences and	10
may not be	10
h and h	10
was mixed with	10
thought to be	10
out of the	10
sets of primers	10
as that of	10
human genomic dna	10
the virus in	10
c for sec	10
two sets of	10
less than h	10
a template for	10
to detect a	10
a thermal cycler	10
virus rapid detection	10
be carried out	10
of lamp was	10
in clinical specimens	10
in combination with	10
swine transmissible gastroenteritis	10
can be applied	10
detection and typing	10
for h n	10
dependent rna polymerase	10
of newcastle disease	10
and identification of	10
it is possible	10
in the absence	10
tomita et al	10
and specific detection	10
and compared with	10
lamp for sars	10
was eluted in	10
amplification assays for	10
mouth disease virus	10
serve as a	10
of the dna	10
in comparison to	10
the sample was	10
of the first	10
isothermal amplification detection	10
lamp method has	10
other respiratory viruses	10
of turkey coronavirus	10
of viral hemorrhagic	10
detection of nvs	10
in table s	10
porcine epidemic diarrhea	10
a member of	10
of the tube	10
agreement with the	10
and typing of	10
work was supported	10
epidermal growth factor	10
was designed to	10
would like to	10
lamp reaction for	10
lateral flow dipstick	10
reactions were carried	10
yellow head virus	10
is able to	10
diagnosis of sars	10
the color change	10
lamp reaction is	10
determine the optimal	10
spread of the	10
at the end	10
the copy number	10
lamp for the	10
hemorrhagic septicaemia virus	10
is a simple	10
to improve the	10
method for detecting	10
and backward inner	10
primers for the	10
and n genes	10
by lamp and	10
depending on the	10
of the detection	10
gargle lavage samples	10
a limit of	10
results were compared	10
development of reverse	10
required for the	10
enterotoxigenic escherichia coli	10
need to be	10
h n avian	10
gene and n	10
of feline coronavirus	10
have been used	10
rna can be	10
of influenza viruses	10
ethics committee of	10
j virol methods	10
assay has been	10
using the rt	10
positive samples were	10
of magnesium pyrophosphate	10
with other respiratory	10
of target dna	10
the activity of	10
growth factor receptor	10
of gene sequences	10
the viral genome	10
of h n	10
a total volume	10
methods for the	10
acid amplification test	10
sensitive than the	10
been reported that	10
detection of products	10
that of rt	9
were heated at	9
a novel nucleic	9
virus in the	9
in the form	9
amplification products were	9
application of a	9
influenza a h	9
none of the	9
were included in	9
detection of dengue	9
in recent years	9
final volume of	9
suitable for the	9
in situ hybridization	9
amplification of nucleic	9
did not cross	9
some of the	9
different concentrations of	9
hydroxynaphthol blue dye	9
with the lamp	9
false positives and	9
dna polymerase and	9
set of six	9
is characterized by	9
by gel electrophoresis	9
a specificity of	9
lamp method for	9
gold standard for	9
results of rt	9
quantifi cation of	9
mycobacterium ulcerans infection	9
at the point	9
detected by the	9
specific for the	9
of cats with	9
reactions were performed	9
to the qpcr	9
with hydroxynaphthol blue	9
rna polymerase promoter	9
carotid artery stenting	9
optical detection unit	9
of feline infectious	9
the results indicated	9
liu et al	9
used for rt	9
n gene and	9
the amplification efficiency	9
we used the	9
primers f and	9
consists of a	9
use in the	9
of rna from	9
a series of	9
of each serotype	9
lamp assay to	9
the conventional rt	9
compared to rt	9
primer set a	9
lateral flow assay	9
the ability of	9
epidemic diarrhea virus	9
specific and sensitive	9
in the early	9
dilution series of	9
of autophagy by	9
of the amplified	9
a single reaction	9
was confirmed by	9
a reverse transcriptase	9
the viral load	9
the best of	9
nucleic acids and	9
patients infected with	9
development of the	9
development of an	9
assay developed in	9
an isothermal amplification	9
a lamp assay	9
compared with conventional	9
ibv or ndv	9
rate of the	9
assay for sars	9
n avian influenza	9
it is also	9
applied to the	9
the rate of	9
of ibv and	9
we evaluated the	9
and control of	9
using a simple	9
the time required	9
repeats of the	9
considered to be	9
in clinical diagnosis	9
pharyngeal swab specimens	9
of escherichia coli	9
room temperature for	9
to identify the	9
the developed rt	9
is the most	9
and reverse transcription	9
disease control and	9
a fast and	9
agarose gel and	9
the assay is	9
total rna was	9
chain reaction and	9
the severe acute	9
using the lamp	9
was assessed by	9
diagnostic methods for	9
to the target	9
been widely used	9
is possible to	9
and bip primers	9
of the method	9
the clinical samples	9
of the test	9
of our knowledge	9
lamp was carried	9
method for covid	9
was extracted using	9
highly specific for	9
a novel reverse	9
a h n	9
is thought to	9
by adding a	9
in the public	9
body cavity effusions	9
reverse transcriptase polymerase	9
high sensitivity of	9
novel reverse transcription	9
n and h	9
the lamp reactions	9
amplification combined with	9
is a major	9
extracted from the	9
as described above	9
diagnostic test for	9
to reduce the	9
a single tube	9
lamp assays for	9
could be a	9
best of our	9
the reaction mixtures	9
of copies of	9
the feasibility of	9
for the identification	9
b lamp assay	9
is required to	9
from red to	9
be found in	9
to the best	9
time pcr system	9
assay was performed	9
the lamp assays	9
of the je	9
influenza a b	9
infected with wild	9
aldh glu lys	9
swine fever virus	9
has been demonstrated	9
were negative by	9
a wide range	9
the advantage of	9
in the target	9
amplified dna products	9
with the same	9
pairs of primers	9
performed as described	9
has a high	9
complementary sequence of	9
and lateral flow	9
is that the	9
assays for the	9
the result of	9
dna by loop	9
detection of salmonella	9
the dna polymerase	9
c for h	9
may be due	9
the possibility of	9
and igg antibodies	9
the amplified dna	9
of a real	9
was purchased from	9
react with other	9
isothermal amplification development	9
the lack of	9
wide range of	9
compared to conventional	9
well as in	9
pcr assay was	9
lamp detection of	9
determined to be	9
pair of primers	9
e gene and	9
resulted in a	9
centrifugal microfluidic system	9
and it is	9
use of lamp	9
zika virus in	9
and cyp c	9
lamp assay on	9
the disposable micro	9
amplifi cation method	8
cell lung cancer	8
time nucleic acid	8
amplification technologies for	8
the potential of	8
to obtain a	8
than the rt	8
lamp method is	8
declare no conflict	8
primers were used	8
a single step	8
amplification can be	8
for the presence	8
used to design	8
efficacy and safety	8
prior to the	8
each sample was	8
in lamp reactions	8
reactions can be	8
hais due to	8
nasopharyngeal swab samples	8
acid amplification technologies	8
to prevent the	8
i influenza a	8
detection of lamp	8
rapid and visual	8
development and application	8
primer sets for	8
part of the	8
activity of the	8
the reaction and	8
difference between the	8
is required for	8
components of the	8
lamp assay had	8
needs to be	8
as the template	8
to the high	8
and nucleic acid	8
reaction was carried	8
newly developed rt	8
were designed based	8
genes of sars	8
products were electrophoresed	8
of egfr mutations	8
ml of the	8
acute ischemic stroke	8
the course of	8
of human influenza	8
negative samples were	8
to determine if	8
c t and	8
serotypes by a	8
serum and urine	8
a temperature of	8
inverted repeats of	8
mechanism of action	8
the utility of	8
a fluorescent dye	8
indicates that the	8
in whole blood	8
there is an	8
mutations in the	8
and the other	8
the first report	8
lamp is more	8
time pcr assay	8
are presented in	8
n genes of	8
the efficiency of	8
those of the	8
caused by the	8
no need for	8
a magnetic bead	8
and lamp assays	8
the hbv lamp	8
of this technique	8
no competing interests	8
control and prevention	8
for quantification of	8
products were analyzed	8
mm each of	8
cdc real time	8
designed based on	8
of less than	8
coronavirus rna in	8
and the sensitivity	8
was not peer	8
function of the	8
isolated from pharyngeal	8
virus infection in	8
of the infection	8
using the same	8
efficiency of the	8
a lateral flow	8
grape powdery mildew	8
for pdm h	8
with the results	8
with clinical samples	8
for reverse transcription	8
time required for	8
of dna template	8
the positive reaction	8
the conventional kit	8
purified dna extracts	8
the s protein	8
hong et al	8
of sry gene	8
tissues and feces	8
f f f	8
phase of infection	8
amplification method rapid	8
we used a	8
the agarose gel	8
acid amplification methods	8
the products of	8
green i was	8
limit of rt	8
member of the	8
is suitable for	8
simultaneous detection of	8
this is the	8
a final volume	8
were visualized by	8
the primer sets	8
with lateral flow	8
of hbv dna	8
of the clinical	8
region of hpv	8
transcription loopmediated isothermal	8
products from the	8
were positive by	8
is similar to	8
highly sensitive and	8
sensitivity of this	8
to create a	8
as the target	8
the reaction can	8
b and b	8
be detected in	8
of reverse transcriptase	8
for the amplification	8
assay detection results	8
the extracted rna	8
formation of a	8
the two assays	8
the lamp products	8
with other viruses	8
a new coronavirus	8
the qiaamp viral	8
and the amplification	8
by means of	8
the gold standard	8
than conventional rt	8
a pair of	8
of dna rapid	8
novel nucleic acid	8
forward and reverse	8
we decided to	8
was subjected to	8
the point of	8
caused by a	8
ct values for	8
the primers used	8
diagnosis for covid	8
the levels of	8
rna was eluted	8
due to ics	8
were detected in	8
detection of hepatitis	8
are needed to	8
have not been	8
in the treatment	8
a combination of	8
which is a	8
on ice for	8
of dengue viruses	8
reaction mixture was	8
time to positivity	8
assay for covid	8
c and d	8
samples obtained from	8
specificity of lamp	8
detection of m	8
and on the	8
the potential to	8
test for the	8
and aldh glu	8
of the pcr	8
positive predictive value	8
all primers were	8
the amplified product	8
perspectives in clinical	8
virus dna by	8
all samples were	8
and does not	8
of l of	8
test the specificity	8
same as that	8
was performed with	8
development of loop	8
amplification assay development	8
of denv infection	8
the rna was	8
with the naked	8
of the covid	8
primers that recognize	8
the sequence of	8
serial dilution of	8
the outbreak of	8
human influenza a	8
that have been	8
higher sensitivity than	8
methods for detection	8
in the laboratory	8
early diagnosis of	8
detection of turkey	8
that were negative	8
lamp assay that	8
on the principle	8
teoh et al	8
direct nucleic acid	8
of lamp products	8
swabs and sputum	8
of coronavirus disease	8
because it is	8
the need of	8
of hpv type	8
the level of	8
of the turbidity	8
when the reaction	8
qiaamp viral rna	8
of primers were	8
without loop primers	8
the products were	8
accordance with the	8
to ensure that	8
amv reverse transcriptase	8
displacement activity and	8
shown to induce	8
assay development and	8
were added to	8
quantitative detection of	8
most widely used	8
is associated with	8
e region of	8
the detection limits	8
the f and	8
and conventional rt	8
of fip and	8
that the assay	8
color of the	8
a commercial rt	8
system for rapid	8
eight distinct regions	8
there was a	8
during the acute	8
of acute gastroenteritis	8
after the reaction	8
lamp method was	8
was developed for	8
authors declare that	8
the reliability of	8
to validate the	8
were synthesized by	8
from to copies	8
by the lamp	8
a positive signal	8
lamp could be	8
red to yellow	8
designed using the	8
b and c	8
corresponding to the	8
the diagnostic laboratory	8
was less than	8
assay is a	8
directly from heat	7
the immune system	7
rna was used	7
with pulmonary adenocarcinoma	7
turkey coronavirus rna	7
as evidenced by	7
is compatible with	7
detection of fcov	7
the mechanism of	7
it is important	7
the ec nv	7
a concentration of	7
the primers and	7
the rapid diagnosis	7
virus isolation and	7
between the two	7
to the standard	7
times more sensitive	7
indicate that the	7
activity of dna	7
for molecular diagnostics	7
study showed that	7
kageyama et al	7
pcr and qrt	7
roles of autophagy	7
copies reaction denv	7
and has been	7
the isothermal mastermix	7
acid amplification using	7
from pharyngeal swabs	7
simple and sensitive	7
isothermal amplification date	7
amplification in the	7
fold more sensitive	7
also known as	7
the b c	7
negative and positive	7
widely used in	7
under field conditions	7
for clinical diagnosis	7
rna in the	7
the reaction temperature	7
mgcl and dntps	7
was found in	7
f and f	7
samples can be	7
amplification was performed	7
be suitable for	7
is highly specific	7
each of f	7
clinical samples was	7
the bst dna	7
been reported in	7
detection limit was	7
of cyp c	7
of the results	7
two pairs of	7
derived from the	7
loop primers were	7
under a author	7
our study was	7
time of min	7
to facilitate the	7
to be involved	7
used for detection	7
to minimize the	7
four sets of	7
of the manuscript	7
great potential for	7
applied to detect	7
sequence of f	7
version of the	7
the lamp reagents	7
lamp assays have	7
was developed by	7
of coronavirus sars	7
details of the	7
the online version	7
used to treat	7
virus genome by	7
isothermal amplification as	7
and centrifuged at	7
ethidium bromide and	7
samples isolated from	7
chikungunya virus by	7
validation of a	7
rna extraction and	7
by the use	7
found that the	7
of lamp assay	7
pcr for the	7
of listeria monocytogenes	7
to prevent cross	7
the acute stage	7
were compared to	7
the ccc pipeline	7
characterization of the	7
of conventional rt	7
of dna accelerated	7
lamp to detect	7
a colorimetric rt	7
between f and	7
by targeting the	7
pcr is the	7
the most important	7
used to identify	7
as templates for	7
by the ethics	7
of the y	7
conditions of the	7
was developed to	7
ll of the	7
sense sequence of	7
accuracy of the	7
a serial dilution	7
white precipitate of	7
developed a rapid	7
is that it	7
and two loop	7
methods have been	7
of f fimbriae	7
final concentration of	7
onset of symptoms	7
sequence based amplification	7
use of rt	7
was obtained from	7
pot visual rt	7
amplification efficiency of	7
samples with ct	7
after the onset	7
nile virus rapid	7
assays for detection	7
constant temperature of	7
to detect rna	7
rna samples isolated	7
of the genus	7
detection of newcastle	7
assay may be	7
dna was detected	7
samples had been	7
is known to	7
and in the	7
science foundation of	7
in conjunction with	7
dependent isothermal dna	7
using colorimetric lamp	7
has been found	7
in peripheral blood	7
of the plasmid	7
stages of the	7
mean detection rates	7
of whole blood	7
applied for the	7
the recent outbreak	7
the rna standard	7
of each of	7
the optimal temperature	7
of lamp and	7
higher than the	7
capable of detecting	7
was the same	7
reaction mixture contained	7
the same strand	7
associated with a	7
are associated with	7
of rna template	7
sensitivity of detection	7
in upper respiratory	7
a final concentration	7
fold higher than	7
similar to that	7
lamp for rapid	7
increase in the	7
the reaction tubes	7
the inner primers	7
the cdc real	7
gene copy number	7
indicating that autophagy	7
was stored at	7
carried out by	7
were suspended in	7
gene amplification technique	7
covert mortality disease	7
lamp assay may	7
compared with that	7
the time of	7
resulting in the	7
can lead to	7
diagnosis of coronavirus	7
products were visualized	7
in cell culture	7
yang et al	7
was performed on	7
proliferative kidney disease	7
the control group	7
the most widely	7
a comparison of	7
that the sensitivity	7
in tissues and	7
generation of innovative	7
dna accelerated reaction	7
are listed in	7
h avian influenza	7
detection of chikungunya	7
role in the	7
in a pcr	7
not suitable for	7
the value of	7
innovative gene amplification	7
using the qiaamp	7
an asymptomatic carrier	7
method rapid detection	7
diagnosis and treatment	7
the activation of	7
rna in tissues	7
human herpesvirus dna	7
for the n	7
detection of rna	7
coronavirus using a	7
detection of potato	7
by polymerase chain	7
molecular test for	7
can be conducted	7
positive results in	7
and the conventional	7
to carry out	7
on the gel	7
of lamp in	7
nasopharyngeal swabs from	7
pcr was carried	7
by electrophoresis on	7
reported in the	7
the f c	7
to analyze the	7
due to a	7
of isothermal nucleic	7
clinical diagnostic laboratory	7
acid sequence based	7
could not be	7
blood samples from	7
regulation of autophagy	7
in the initial	7
to provide a	7
can be easily	7
lamp reaction mixtures	7
of salmonella enterica	7
of each sample	7
we report a	7
to amplify the	7
reverse transcription loopmediated	7
addition to the	7
by a reverse	7
between the rt	7
lamp primer design	7
lamp in a	7
by the loop	7
detection of hpv	7
were mixed with	7
the growing season	7
immunodeficiency virus type	7
resulting in a	7
patients with pulmonary	7
an independent cohort	7
six distinct sequences	7
when detecting ibv	7
gel electrophoresis or	7
the clinical diagnosis	7
present study was	7
been reported to	7
surface plasmon resonance	7
was positive in	7
pcr detection of	7
the positive control	7
of this article	7
concentrations of primers	7
negative predictive value	7
belong to the	7
can be visualized	7
high degree of	7
of buruli ulcer	7
amplification rapid detection	7
has been described	7
the type of	7
of mthfr c	7
were designed from	7
dna extracted from	7
enzymatic amplification of	7
in the development	7
following intravenous alteplase	7
reaction mixtures were	7
lamp amplifi cation	7
positive by rt	7
like to thank	7
detection based on	7
amplification development of	7
isothermal amplification combined	7
primer set for	7
to examine the	7
directly from clinical	7
forward loop primer	7
lamp and prv	7
be applied for	7
method for infectious	7
detecting ibv and	7
the plate was	7
lamp and conventional	7
of the fip	7
were confirmed by	7
sequence of b	7
and there is	7
has the advantage	7
in the current	7
at the same	7
the master mix	7
lamp had a	7
as a negative	7
simple method for	7
detection of feline	7
the target snp	7
at r min	7
the establishment of	7
early stages of	7
and the lamp	7
on the lamp	7
committee of the	7
clinical application of	7
is essential for	7
kit for the	7
wang et al	7
automated centrifugal microfluidic	7
method can be	7
compared to pcr	7
the prv ge	7
reactivity with other	7
for cyp c	7
sets were designed	7
of the study	7
this study showed	7
products can be	7
highly sensitive detection	7
direct pcr amplification	7
the y chromosome	7
effective diagnostic method	7
the template input	7
dna amplification and	7
alere i influenza	7
f c and	7
and west nile	7
that it is	7
clinical samples and	7
ms and rf	7
the ethics committee	7
there were no	7
of innovative gene	7
equipment such as	7
a author funder	7
subjected to rt	7
syndrome coronavirus rapid	7
performed in the	7
all of these	7
in a reaction	7
of the newly	7
the genome of	7
amount of dna	7
of the denv	7
correlation between the	7
assay using a	7
these samples were	7
summarized in table	7
a negative control	7
amplification as a	7
reaction volume containing	6
rna using colorimetric	6
diagnosis of dengue	6
gel electrophoresis was	6
by the national	6
for virus detection	6
viral rna mini	6
detection of tomato	6
to design the	6
dna from the	6
specificity for the	6
swab samples were	6
were defined as	6
can provide a	6
based rna extraction	6
primers fip and	6
foundation of china	6
anneals to the	6
our previous study	6
techniques have been	6
of dna with	6
was not detected	6
amplification of rna	6
autophagy has been	6
a viruses by	6
detected in samples	6
it suitable for	6
depicted in figure	6
the lysis buffer	6
of c for	6
patients with pneumonia	6
size of the	6
distilled water and	6
have indicated that	6
virus by real	6
was less sensitive	6
well plate was	6
detection of swine	6
gunimaladevi et al	6
x primer mix	6
primers used for	6
the ns gene	6
detection in the	6
essential for the	6
and reverse primers	6
the primers are	6
n and n	6
advantage of the	6
pcr as a	6
institutional review board	6
studies have shown	6
qlamp assay detection	6
lower than that	6
values for the	6
loops formed by	6
creative commons attribution	6
time reverse transcriptase	6
can be an	6
reaction volume of	6
times higher than	6
the restriction enzyme	6
the established rt	6
been developed and	6
can be carried	6
as a means	6
number of rna	6
x master mix	6
sets of lamp	6
its ability to	6
carried out with	6
and sputum samples	6
used to amplify	6
rna extraction kits	6
multiple displacement amplification	6
described in section	6
developed for detection	6
including two outer	6
dna amplification in	6
pacific white shrimp	6
step reverse transcription	6
characteristics of the	6
positive reaction was	6
the cap of	6
chronic hepatitis b	6
negative by rt	6
declare that they	6
both the rt	6
reverse transcriptase loop	6
colorimetric lamp assay	6
suggested that the	6
also be used	6
assay does not	6
of infection and	6
detection of dna	6
higher sensitivity and	6
be observed by	6
and the prv	6
concentration of the	6
independent cohort of	6
in health and	6
simple water bath	6
and had a	6
suitable for use	6
science and technology	6
in the cap	6
amplification reaction for	6
encephalitis virus by	6
peripheral blood mononuclear	6
sequence analysis of	6
of copies reaction	6
is expected to	6
revealed that the	6
bronchoalveolar lavage fluid	6
were negative for	6
lamp assay could	6
bhadra et al	6
other groups have	6
the pi k	6
it does not	6
warmstart colorimetric lamp	6
of dna polymerase	6
assay in the	6
assay could be	6
relies on the	6
this article can	6
we would like	6
the impact of	6
refers to the	6
in blood samples	6
plasma and serum	6
viral rna detection	6
of respiratory syncytial	6
be involved in	6
positive for h	6
performed on the	6
has been successfully	6
a critical review	6
designed from the	6
informed consent was	6
with a low	6
developed for the	6
isothermal amplification kit	6
in line with	6
and serotyping of	6
is more sensitive	6
with ml of	6
internal carotid artery	6
as low as	6
fl uorescent dye	6
developed to detect	6
targeting the e	6
by visual inspection	6
is important to	6
small sample size	6
an outbreak of	6
lamp reaction products	6
each of primers	6
it shows that	6
of loopmediated isothermal	6
for snp detection	6
autophagy in cancer	6
of mgcl and	6
relative to the	6
with a sensitivity	6
autophagy is a	6
to have a	6
using a portable	6
in a biosafety	6
used as an	6
limit of copies	6
was applied to	6
nucleic acids with	6
when compared to	6
reliable detection of	6
in the assay	6
amplification using ph	6
was measured by	6
also been used	6
strand displacement reaction	6
of the real	6
may be used	6
feces by reverse	6
and highly sensitive	6
on the sample	6
amplification test for	6
detection of various	6
for lamp and	6
negative diagnosis for	6
confirmed by sequencing	6
inhibition of autophagy	6
the world health	6
and quantifi cation	6
can be read	6
and did not	6
by centrifugation at	6
reliability of the	6
for amplification of	6
emerging infectious diseases	6
assay should be	6
detection of a	6
the control line	6
with ct values	6
been applied for	6
molecular diagnostic test	6
reverse transcription step	6
the mean detection	6
sample to result	6
is an isothermal	6
a novel method	6
the ap pathway	6
by a novel	6
autophagic cell death	6
other related viruses	6
assay and lamp	6
of nested rt	6
in the growing	6
dna was eluted	6
of the direct	6
diagnostic potential of	6
the aim of	6
detection of african	6
viral load of	6
with the use	6
based on these	6
culture medium and	6
lamp method can	6
test for sars	6
that the primers	6
for sec and	6
and copies of	6
the cyp c	6
sample preparation and	6
appears to be	6
a valuable tool	6
the host cell	6
mediated isothermal amplificatio	6
on the same	6
which results in	6
from the rt	6
copies of is	6
be adapted to	6
was established by	6
a method for	6
methods for detecting	6
in vitro and	6
of the beads	6
the primer sequences	6
targeting orf a	6
up to fold	6
slit lamp shield	6
visualization of the	6
crispr cas a	6
of the mixture	6
positive for sars	6
to inhibit autophagy	6
without dna extraction	6
that of pcr	6
early stage of	6
the virus and	6
shown to increase	6
gene of sars	6
the positive samples	6
reported that the	6
acidification of the	6
for orf a	6
gastroenteritis in the	6
with conventional rt	6
alternately inverted repeats	6
of these samples	6
consists of the	6
selected as the	6
of the colorimetric	6
heidelberg university hospital	6
large amount of	6
lamp reaction with	6
a similar sensitivity	6
lamp assay detected	6
flp and blp	6
are used to	6
false negative results	6
white spot syndrome	6
around the world	6
clinical specimens from	6
buffer and stored	6
they have no	6
nucleotide sequence of	6
are used for	6
article can be	6
r min for	6
determined by the	6
may be a	6
incubated in a	6
this article is	6
and incubated for	6
spot syndrome virus	6
positive results were	6
and subjected to	6
effusions of cats	6
detection of isothermal	6
and orf ab	6
time pcr in	6
was tested using	6
a and the	6
thai et al	6
laboratory confirmation of	6
diagnostic tool for	6
can be achieved	6
stepone tm system	6
small cell lung	6
to be highly	6
volume of l	6
of samples with	6
ecas for hais	6
detection of zikv	6
sensitivities of the	6
c a a	6
detection and quantitation	6
molecular and serological	6
this method is	6
set for the	6
copies of sars	6
analytical sensitivity of	6
detection of norovirus	6
for samples with	6
rna extraction for	6
time polymerase chain	6
have been identified	6
of chik fever	6
with those obtained	6
and no cross	6
no template control	6
this case report	6
to the detection	6
beclin and atg	6
at least one	6
incubated at c	6
as determined by	6
risk of contamination	6
sensitive than pcr	6
blood mononuclear cells	6
with a lateral	6
amplifi cation is	6
need for expensive	6
optimization of the	6
samples were obtained	6
of hcv rna	6
answer detection of	6
provided by dr	6
and reaction time	6
did not show	6
during the reaction	6
cats with and	6
hepatitis a virus	6
a biosafety level	6
followed by the	6
evaluation of loop	6
determine the sensitivity	6
as mentioned above	6
and feces by	6
swab samples from	6
isothermal amplification loop	6
of the severe	6
sars and mers	6
in our previous	6
identification of the	6
also been shown	6
two inner primers	6
sent to the	6
is complementary to	6
the detection system	6
similar to the	6
during the early	6
and the n	6
as an alternative	6
clinical evaluation of	6
reverse transcription pcr	6
commercial rna extraction	6
primers used in	6
at z c	6
gene specific rt	6
at a single	6
to be positive	6
igg antibodies in	6
treated with naoh	6
lamp master mix	6
genome by loop	6
all reactions were	6
were purchased from	6
we tested the	6
molecular characterization of	6
in this case	6
of n gene	6
based on its	6
detection of egfr	6
of cellular autophagy	6
it is a	6
ensure that the	6
amplification method that	6
and minor gii	6
lamp was found	6
of z c	6
of the fluorescent	6
added to each	6
the tubes were	6
to detect and	6
methods to detect	6
lamp reaction can	6
of swab samples	6
lowest detection limits	6
gel stained with	6
virus rapid and	6
reactions were incubated	6
by the institutional	6
as compared to	6
for the prevention	6
of template rna	6
number of samples	6
amplification assay rapid	6
added into the	6
mixtures were incubated	6
cap of the	6
or heat block	6
of the genome	6
open reading frame	6
from sample collection	6
detection of viruses	6
of this rt	6
is difficult to	6
of the b	6
the containment of	6
time pcr and	6
were positive in	6
and accurate diagnosis	6
studies have reported	6
and ml of	6
u of bst	6
detection with crispr	6
at the poc	6
detection rates of	6
was selected as	6
were extracted from	6
use of loop	6
detection system for	6
to detect all	6
to accelerate the	6
iwamoto et al	6
by measuring the	6
novel coronavirus from	6
both autophagy and	6
g g type	6
and easy detection	6
with pneumonia in	6
or heating block	6
that were positive	6
a simple water	6
dna polymerase to	6
zika virus infection	6
ability to amplify	6
consisted of the	6
be performed using	6
of the rapid	6
human papillomavirus type	6
on the detection	6
of dna development	6
responsible for the	6
obtained from patients	6
with a single	6
monocytogenes dna template	6
able to amplify	6
lamp amplification products	6
in vitro transcription	6
number of clinical	6
belongs to the	6
is performed in	6
structure of the	6
results were observed	6
traditional chinese medicine	6
have shown that	6
that they have	6
nasopharyngeal swabs and	6
abbott id now	6
the introduction of	6
n gene was	6
a dilution series	6
to establish a	6
serum samples were	6
ml reaction volume	6
the pocket warmer	6
used for lamp	6
the method of	6
assay as a	6
has the potential	6
a report of	6
products were separated	6
the sensitivity was	6
we did not	6
chinese academy of	6
approved the final	6
t and aldh	6
morbidity and mortality	6
amplification methods for	6
in this paper	6
min in the	6
was lower than	6
method for diagnosis	6
the loop primer	6
the magnesium pyrophosphate	6
to the rt	6
the early stages	6
using an isothermal	6
been successfully applied	6
and the detection	6
assay was evaluated	6
presence of the	6
of h avian	6
lei et al	6
of the cases	6
that was developed	6
s and s	6
acid detection with	6
explorer v software	6
in a final	6
bath or heat	6
a very low	6
in figure a	6
rna mini kit	6
the application of	6
detection of chlamydia	6
ice for min	6
a sensitive and	6
and validation of	6
with the conventional	6
green i dye	6
for the reaction	6
and the assay	6
zheng et al	6
study was supported	6
presence of a	6
potential of rt	6
the results are	6
we found that	6
the robustness of	6
dilution of the	6
samples using the	6
to perform the	6
the minimum concentration	6
template for rt	6
were tested using	6
used in a	6
were collected in	6