quadgram

This is a table of type quadgram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

quadgram	frequency
severe acute respiratory syndrome	413
middle east respiratory syndrome	297
acute respiratory syndrome coronavirus	266
east respiratory syndrome coronavirus	240
is the author funder	174
display the preprint in	164
license to display the	164
the preprint in perpetuity	164
who has granted medrxiv	164
to display the preprint	164
medrxiv a license to	164
a license to display	164
granted medrxiv a license	164
has granted medrxiv a	164
the copyright holder for	161
copyright holder for this	161
holder for this preprint	160
this preprint this version	150
for this preprint this	150
preprint this version posted	150
of severe acute respiratory	135
of the s protein	122
made available under a	121
it is made available	121
is made available under	121
of the spike protein	116
a is the author	112
international license it is	111
available under a is	111
license it is made	111
under a is the	111
the receptor binding domain	98
was not certified by	87
not certified by peer	87
certified by peer review	87
the spike protein of	81
the s protein of	79
which was not certified	77
spike protein of sars	75
of middle east respiratory	71
em structure of the	70
tf tf tf tf	66
structure of the sars	62
in the presence of	60
the severe acute respiratory	60
in the prefusion conformation	59
on the surface of	59
the rbd of sars	58
on the other hand	55
in the case of	54
r n a l	52
a l p r	52
l p r e	52
r o o f	52
spike in the prefusion	52
respiratory syndrome coronavirus spike	52
antigenicity of the sars	52
o u r n	52
j o u r	52
p r o o	52
u r n a	52
n a l p	52
and antigenicity of the	51
a functional receptor for	50
ncov spike in the	50
in the absence of	49
the rbd of the	49
the crystal structure of	48
s protein of sars	48
to the ace receptor	48
is a functional receptor	47
the binding of the	46
binding domain complexed with	46
is protected by copyright	46
structural basis for the	46
article is protected by	46
this article is protected	46
as shown in figure	46
at room temperature for	45
entry into host cells	44
this version posted august	44
syndrome coronavirus spike protein	44
entry depends on ace	43
blocked by a clinically	43
tmprss and is blocked	43
for the treatment of	43
by a clinically proven	43
depends on ace and	43
and is blocked by	43
and tmprss and is	43
a clinically proven protease	43
is blocked by a	43
ace and tmprss and	43
on ace and tmprss	43
for the recognition of	42
clinically proven protease inhibitor	42
cell entry depends on	42
functional receptor for the	42
antibody responses to sars	41
of the spike glycoprotein	40
was added to the	39
no reuse allowed without	38
in the context of	38
the d g mutation	38
of a novel coronavirus	38
reuse allowed without permission	38
of the s subunit	38
as well as the	37
domain of the spike	37
basis for the recognition	37
binding domain of the	37
as shown in fig	37
this version posted july	36
receptor binding domain of	36
n q n q	36
of the sars coronavirus	36
bound to the ace	36
with severe acute respiratory	36
the viral spike protein	35
domain bound to the	34
s and s subunits	34
q n q n	34
respiratory syndrome coronavirus infection	34
in the spike protein	33
crystal structure of the	33
the middle east respiratory	33
binding domain bound to	33
coronavirus of probable bat	32
associated with a new	32
with a new coronavirus	32
was found to be	32
pneumonia outbreak associated with	32
for the development of	32
new coronavirus of probable	32
a new coronavirus of	32
outbreak associated with a	32
a pneumonia outbreak associated	32
of probable bat origin	32
neutralizing antibodies against sars	31
of coronavirus spike proteins	31
the interaction between the	31
h at room temperature	31
the protein data bank	31
incubated for h at	30
the s and s	30
receptor for the sars	30
the rbd domain of	30
the recognition of sars	29
in complex with the	29
the s subunit of	29
has been shown to	29
a human monoclonal antibody	29
the surface of the	28
the structure of the	28
the s protein is	28
a novel coronavirus from	28
the authors declare no	27
into the host cell	27
for h at room	27
of sars coronavirus spike	27
this version posted october	27
entry into the host	26
the binding affinity of	26
binding domain of mers	26
of the s trimer	26
of the viral spike	26
were found to be	26
for the sars coronavirus	26
in patients with covid	26
for the detection of	26
binding domain of sars	26
by human monoclonal antibodies	26
in the s subunit	26
the s subunit and	26
in the s protein	25
on the s protein	25
enzyme is a functional	25
was used as a	25
of convalescent plasma therapy	25
converting enzyme is a	24
functional basis of sars	24
and functional basis of	24
shown in figure a	24
s protein of the	24
the s proteins of	24
to the rbd of	24
in a single inoculum	24
of patients infected with	24
structural and functional basis	24
at a concentration of	24
rbd in complex with	24
binding of the rbd	23
the ace binding site	23
entry by using human	23
washed three times with	23
novel coronavirus from wuhan	23
by using human ace	23
added to each well	23
the novel coronavirus from	23
the rbd in the	23
at the s s	23
incubated at room temperature	22
acute respiratory distress syndrome	22
was carried out using	22
the rbd and the	22
a wide range of	22
of the rbd to	22
to that of the	22
to the host cell	22
the spike glycoprotein rbd	22
between the rbd and	21
of receptor recognition by	21
binding to the rbd	21
structural basis of receptor	21
the host cell receptor	21
s in supplementary material	21
as a function of	21
of the severe acute	21
structures of the sars	21
basis of receptor recognition	21
an important role in	21
porcine epidemic diarrhea virus	21
receptor recognition by sars	21
the human ace receptor	21
with ic values of	21
of the rbd of	20
in vitro and in	20
glycoproteins reveal the dynamic	20
conserved cryptic epitope in	20
days after symptom onset	20
an analysis based on	20
the dynamic receptor binding	20
dynamic receptor binding domains	20
highly conserved cryptic epitope	20
cov spike glycoproteins reveal	20
an ic value of	20
by the novel coronavirus	20
min at room temperature	20
the context of the	20
against middle east respiratory	20
in patients with severe	20
work was supported by	20
epitope in the receptor	20
rbd of the s	20
structure of sars coronavirus	20
the presence of the	20
structural studies of sars	20
evolution of coronavirus spike	20
reveal the dynamic receptor	20
and incubated for h	20
spike glycoproteins reveal the	20
as compared to the	20
a highly conserved cryptic	20
cryptic epitope in the	20
domain complexed with receptor	20
em structures of mers	20
receptor recognition by the	19
coronavirus spike protein is	19
rbd and the ace	19
on the basis of	19
the world health organization	19
binding domains of sars	19
the host cell membrane	19
are shown in figure	19
crystal structure of sars	19
long structural studies of	19
and evolution of coronavirus	19
analysis based on decade	19
authors declare no competing	19
the interaction of the	19
shown in figure b	19
spike glycoprotein of sars	19
in accordance with the	19
were added to the	19
figure s in supplementary	19
for min at room	19
clinical features of patients	19
features of patients infected	19
for severe acute respiratory	18
were washed three times	18
in the up conformation	18
the sars coronavirus spike	18
is one of the	18
of the rbd and	18
the spike glycoprotein of	18
by the addition of	18
this work was supported	18
sars coronavirus spike receptor	18
with an ic of	18
mbcs reactive to the	18
patients infected with novel	18
with the ace receptor	18
of the receptor binding	18
of the novel coronavirus	18
highly potent neutralizing antibodies	18
for the presence of	18
by a human monoclonal	18
with novel coronavirus in	18
than that of the	18
these results indicate that	18
a final concentration of	18
a large number of	18
recognition by the novel	18
infected with novel coronavirus	18
residues in the rbd	18
after onset of symptoms	17
these data suggest that	17
binding to its receptor	17
was used for the	17
the binding of rbd	17
with an ic value	17
potent neutralizing antibodies against	17
spike protein is a	17
these results suggest that	17
prevention and treatment of	17
a man with pneumonia	17
the plates were washed	17
binding of rbd to	17
the s protein and	17
a small animal model	17
the binding of sars	17
interaction with mab h	17
rbd of the sars	17
for each of the	17
coronavirus spike protein by	17
was added to each	17
respiratory syndrome coronavirus in	17
binding of the s	17
one of the most	17
the n k mutation	17
of human neutralizing antibodies	17
cells were infected with	17
be due to the	17
the majority of the	17
is shown in figure	16
as a negative control	16
structures of a rationally	16
this version posted june	16
a rationally designed prefusion	16
rationally designed prefusion mers	16
at the same time	16
in complex with ace	16
isolation of a novel	16
man with pneumonia in	16
host cell receptor ace	16
coronavirus from a man	16
neutralizing antibodies elicited by	16
crystal structures of the	16
state for receptor binding	16
the ability of the	16
novel coronavirus in wuhan	16
rbd domain of the	16
of a rationally designed	16
immunogenicity and structures of	16
structural and functional characterization	16
the rbd of mers	16
s subunit of the	16
of the d g	16
the sars coronavirus s	16
from a man with	16
the coronavirus spike protein	16
the development of a	16
of the fusion core	16
in addition to the	16
have been shown to	16
human respiratory disease in	16
sars coronavirus s protein	16
is a class i	16
human neutralizing antibodies elicited	16
pneumonia in saudi arabia	16
and structures of a	16
to bind to the	16
novel coronavirus from a	16
antibodies elicited by sars	16
with pneumonia in saudi	16
against the s protein	16
for the emerging human	15
of a coronavirus spike	15
a novel coronavirus associated	15
and the s subunit	15
followed by addition of	15
receptor for the emerging	15
spike protein by a	15
prerequisite conformational state for	15
the s domain of	15
electron microscopy structures of	15
conformational state for receptor	15
cov spike glycoprotein reveal	15
a prerequisite conformational state	15
and incubated for min	15
binding site of sars	15
dipeptidyl peptidase is a	15
in complex with its	15
block the binding of	15
functional receptor for sars	15
declare no competing interests	15
binding of novel coronavirus	15
functional characterization of the	15
the stability of the	15
peptidase is a functional	15
structural basis for potent	15
a new coronavirus associated	15
with human respiratory disease	15
used in this study	15
rights reserved this article	15
microscopy structures of the	15
associated with human respiratory	15
serological assay to detect	15
the case of the	15
assay to detect sars	15
for binding to the	15
new coronavirus associated with	15
novel coronavirus associated with	15
coronavirus associated with human	15
studies of sars coronavirus	15
the authors declare that	15
in the rbd of	15
novel coronavirus spike protein	15
we observed that the	15
all rights reserved this	15
protein by a sars	15
we found that the	15
in the receptor binding	15
of novel coronavirus spike	15
a serological assay to	15
and functional characterization of	15
class i virus fusion	15
fusion of the viral	15
spike glycoprotein reveal a	15
the emerging human coronavirus	15
potent binding of novel	15
novel human coronavirus mers	15
reserved this article is	15
glycoprotein in complex with	14
in the development of	14
in the sera of	14
to each well and	14
vitro and in vivo	14
at the end of	14
vaccine and therapeutic development	14
protein is a class	14
sites under positive selection	14
in the united states	14
coronavirus associated with severe	14
a target for vaccine	14
for vaccine and therapeutic	14
and evolution of pathogenic	14
igg mbcs reactive to	14
is consistent with the	14
bind to the rbd	14
the presence or absence	14
of the s glycoprotein	14
it is likely that	14
the combinatorial adjuvant system	14
was supported by the	14
presence or absence of	14
origin and evolution of	14
are shown in table	14
antibody response to sars	14
critically ill patients with	14
patients with severe acute	14
the emergence of sars	14
room temperature for h	14
spike glycoprotein in complex	14
the end of the	14
a class i virus	14
hr and hr domains	14
characterization of the fusion	14
evolution of pathogenic coronaviruses	14
glycoprotein reveal a prerequisite	14
target of neutralizing antibodies	14
i virus fusion protein	14
viral entry into host	14
the effect of the	14
to the human ace	14
for the production of	14
to the s subunit	14
reveal a prerequisite conformational	14
the recognition of the	14
s subunit of sars	14
of the novel human	14
it has been shown	13
with the human ace	13
this version posted may	13
the s protein to	13
incubated for min at	13
that the s protein	13
cells were incubated with	13
viral entry into the	13
for potent neutralization of	13
to its receptor ace	13
are available from the	13
this version posted september	13
target for vaccine and	13
isolation and characterization of	13
specific human monoclonal antibody	13
convalescent plasma therapy in	13
the viral s protein	13
s protein is the	13
of the immune response	13
to induce neutralizing antibodies	13
associated with severe acute	13
the fusion core complex	13
the plates were incubated	13
neutralization of sars coronavirus	13
neutralizing antibodies block covid	13
was used as the	13
days after onset of	13
the prefusion conformation structure	13
at the interface of	13
terminal domain of the	13
the binding free energy	13
from the protein data	13
are presented as mean	13
epitope on the rbd	13
a noncompeting pair of	13
human neutralizing antibodies block	13
igg against the sars	13
basis for potent neutralization	13
pair of human neutralizing	13
virus binding to its	13
fragment of the sars	13
with the host cell	13
similar to that of	13
conformational changes in the	13
human monoclonal antibody blocking	13
of the immune system	13
to interact with the	13
the limit of detection	13
of porcine epidemic diarrhea	13
respiratory disease in china	13
the s protein in	13
is mediated by the	13
protein of severe acute	13
adjuvanted rbd vaccine in	13
in a small animal	13
the fact that the	13
the neutralizing activity of	13
by size exclusion chromatography	13
monoclonal antibody blocking sars	13
to the s protein	13
between the s protein	13
no conflict of interest	13
sars coronavirus spike glycoprotein	13
it is important to	13
did not bind to	13
the rbd and s	13
spike receptor binding domain	13
noncompeting pair of human	13
from patients with pneumonia	13
on the spike protein	13
rbd vaccine in separate	13
s subunit and the	13
amino acid substitutions in	13
at the binding interface	13
rbd binding to ace	13
implications for virus origins	12
the onset of symptoms	12
a coronavirus spike glycoprotein	12
virus origins and receptor	12
compared to that of	12
neutralizing human monoclonal antibody	12
homology modelling of protein	12
results indicate that the	12
protection from disease in	12
the receptor binding domains	12
free energies of binding	12
a single amino acid	12
the spike glycoprotein and	12
of cell entry and	12
levels of igg against	12
with the rbd of	12
modelling of protein structures	12
rbd and s subunit	12
highly specific target of	12
the development of vaccines	12
the receptor binding motif	12
female balb c mice	12
novel coronavirus from patients	12
acid fragment of the	12
adaptation to human ace	12
and protection from disease	12
involved in the interaction	12
the prevention and treatment	12
protein structures and complexes	12
domain of the viral	12
that the d g	12
human angiotensin converting enzyme	12
the rbds of sars	12
be used as a	12
development of vaccines and	12
potent neutralization of sars	12
to a final concentration	12
the causative agent of	12
is responsible for the	12
was measured at nm	12
immune response to sars	12
plates were coated with	12
data are presented as	12
as well as a	12
the case of sars	12
neutralizing antibodies and protection	12
domain complexed with its	12
of the s rbd	12
after the onset of	12
monoclonal antibody combination against	12
in the crystal structure	12
could be due to	12
antibodies and protection from	12
of neutralizing antibodies against	12
disease in a small	12
of angiotensin converting enzyme	12
residues involved in the	12
in the present study	12
assessment of cell entry	12
and epidemiology of novel	12
a key role in	12
from disease in a	12
of the rbd domain	12
for the prevention of	12
in the human population	12
and postexposure efficacy of	12
inactivated h n pdm	12
rbd of the spike	12
as part of the	12
plates were incubated at	12
to that of sars	12
receptor and viral determinants	12
of sb and sb	12
coronavirus s protein efficiently	12
isolation of potent sars	12
with middle east respiratory	12
humoral and cellular immunity	12
with pneumonia in china	12
d g a s	12
it was found that	12
of protein structures and	12
the entry of sars	12
each well and incubated	12
cell sequencing of convalescent	12
can be used as	12
and incubated at room	12
coronavirus from patients with	12
that bind to the	12
s protein efficiently binds	12
origins and receptor binding	12
results showed that the	12
binding to the ace	12
epidemiology of novel coronavirus	12
higher than that of	12
a human monoclonal sars	12
human monoclonal antibody combination	12
ter meulen et al	12
entry and receptor usage	12
functional assessment of cell	12
patients with pneumonia in	12
it is possible that	12
rbd protein of sars	12
the binding of mers	12
that the n k	12
for virus origins and	12
cell entry and receptor	12
and receptor usage for	12
convergent antibody responses to	12
and viral determinants of	12
antibody combination against sars	12
to the presence of	11
of the virus and	11
added and incubated for	11
use of convalescent plasma	11
potent neutralization of betacoronaviruses	11
neutralization of betacoronaviruses by	11
amino acid fragment of	11
for rapid detection of	11
for development of rbd	11
vaccine in separate sites	11
from mice immunized with	11
coronavirus spike protein contains	11
structure of a human	11
h n pdm and	11
cov spike protein induces	11
in the range of	11
s protein binds to	11
basis of binding between	11
is an immunodominant and	11
with the ace binding	11
region of the s	11
with that of sars	11
of critically ill patients	11
receptor usage for sars	11
spike protein is an	11
sars coronavirus spike protein	11
coronavirus adaptation to human	11
this version posted april	11
complexed with human receptor	11
in the presence or	11
and coverage of escape	11
a visualization system for	11
hour at room temperature	11
in the respiratory tract	11
for exploratory research and	11
of igg against the	11
the crystal structures of	11
for binding to rbd	11
as a viral attachment	11
for macromolecular structure solution	11
with acute respiratory distress	11
between novel human coronavirus	11
attachment inhibitor and vaccine	11
cells were transfected with	11
binding between novel human	11
in the current study	11
heavy and light chains	11
implication for development of	11
development of rbd protein	11
as severe acute respiratory	11
s s cleavage site	11
target of antibodies in	11
combination against sars coronavirus	11
system for exploratory research	11
protein as a viral	11
of binding between novel	11
room temperature for min	11
specificity and sensitivity of	11
its host cell receptor	11
recognition of the sars	11
domain complexed with human	11
electron microscopy structure of	11
tools for molecular graphics	11
the viral spike glycoprotein	11
in hek t cells	11
analysis was performed using	11
coverage of escape mutants	11
of rbd protein as	11
with fetal bovine serum	11
for the prevention and	11
of spike glycoprotein of	11
and its receptor cd	11
and the cells were	11
of antibodies in sars	11
molecular basis of binding	11
rbd protein as a	11
for the s protein	11
the immunogenicity of the	11
the specificity of the	11
the human igg fc	11
of neutralizing antibodies in	11
a viral attachment inhibitor	11
hek t cells were	11
the furin cleavage site	11
cov and its receptor	11
system for macromolecular structure	11
carried out using the	11
mice immunized with rbd	11
immunodominant and highly specific	11
the rest of the	11
based system for macromolecular	11
the s protein rbd	11
for middle east respiratory	11
by severe acute respiratory	11
epitopes on the rbd	11
h n influenza virus	11
with human receptor dpp	11
analyses were performed using	11
administered in a single	11
specific target of antibodies	11
cleavage of the s	11
analysis of the sars	11
key residues in the	11
that the binding of	11
specific humoral and cellular	11
protein in complex with	11
of viral load in	11
the steepest descent algorithm	11
an immunodominant and highly	11
viral attachment inhibitor and	11
characterisation and epidemiology of	11
exploratory research and analysis	11
has been shown that	11
the absence of the	11
visualization system for exploratory	11
viral spike protein is	11
synergy and coverage of	11
was determined by the	11
the cells were then	11
viral determinants of sars	11
other lineage b betacoronaviruses	11
spike protein and the	11
immune responses to sars	11
and other lineage b	11
protein is an immunodominant	11
vaccines and antibody therapies	11
and highly specific target	11
coronavirus fusion inhibitor targeting	11
aminopeptidase n is a	11
rbd with high affinity	11
this study are available	11
genomic characterisation and epidemiology	11
respiratory syndrome coronavirus receptor	11
potent neutralizing antibodies from	10
the humoral immune response	10
to the cell surface	10
block the interaction of	10
the spike protein and	10
monoclonal antibody against mers	10
is an urgent need	10
a small number of	10
long ace isoforms in	10
were carried out using	10
in the s domain	10
fusion structure of a	10
the reference ssaa ligand	10
binding affinity to the	10
of the host cell	10
building tools for molecular	10
between april and june	10
and naming it sars	10
flexibility of upward rbd	10
of neutralizing monoclonal antibodies	10
statistical analyses were performed	10
s subunit of s	10
ncov and naming it	10
and the rbd of	10
the spike protein in	10
binding affinity of the	10
in unexposed subjects and	10
coronavirus spike glycoprotein in	10
of novel coronavirus disease	10
to the spike protein	10
are shown in fig	10
table and supplementary fig	10
for their ability to	10
is essential for the	10
with the receptor binding	10
the s subunit is	10
of vero e cells	10
a s d g	10
of betacoronaviruses by single	10
of a human coronavirus	10
identification of a novel	10
a human coronavirus spike	10
did not show any	10
human coronavirus spike protein	10
the ntd and rbd	10
binding to the host	10
a limited number of	10
in this study were	10
available under a the	10
a critical role in	10
by a sars coronavirus	10
administered in separate sites	10
receptor binding domains of	10
to the development of	10
b cell response to	10
authors declare no conflict	10
and its interaction with	10
and clinical characteristics of	10
there is an urgent	10
the wild type rbd	10
complex with its host	10
with its host cell	10
the s s cleavage	10
for h at rt	10
a web server for	10
the receptor binding site	10
the species severe acute	10
ray crystal structure of	10
were expressed and purified	10
a significant increase in	10
license was not certified	10
than that of sars	10
was performed using the	10
international license was not	10
the interface of the	10
a human neutralizing antibody	10
and the receptor binding	10
plates were incubated for	10
in agreement with the	10
the conformation of the	10
of the ace receptor	10
the side chain of	10
the rbd a c	10
we have previously shown	10
targeting the hr domain	10
simulations were carried out	10
entry into target cells	10
this is consistent with	10
of rbd of sars	10
between the two proteins	10
studies have shown that	10
as a target for	10
these observations suggest that	10
which this version posted	10
transgenic mice from mers	10
in contrast to the	10
between hace and rbd	10
the results showed that	10
a the copyright holder	10
a time step of	10
respiratory syncytial virus infection	10
a key target for	10
was first reported in	10
structure of the rbd	10
short ace isoforms in	10
at nm was measured	10
species severe acute respiratory	10
the result showed that	10
we did not detect	10
in a mouse model	10
by the presence of	10
of the s domain	10
in middle east respiratory	10
amino acid residues in	10
with the spike glycoprotein	10
not included in the	10
adjuvanted rbd vaccine were	10
under a the copyright	10
patients with coronavirus disease	9
was obtained from the	9
available from the corresponding	9
sars coronavirus isolates by	9
its interaction with the	9
to the wild type	9
a potential target for	9
protein receptor binding domain	9
s p and s	9
domain complexed with neutralizing	9
should be noted that	9
experiments were performed in	9
target for vaccine development	9
induce highly potent neutralizing	9
not bind to the	9
complexed with its human	9
time step of fs	9
as well as to	9
that d g increases	9
for the expression of	9
entry mechanisms of sars	9
some protection against sars	9
neutralizing antibodies from covid	9
proteins of severe acute	9
to the human receptor	9
can be divided into	9
the viral entry into	9
interaction between the s	9
of the virus to	9
the treatment of covid	9
human tissues using clinical	9
tracking changes in sars	9
neutralizing epitopes in the	9
convalescent subjects than in	9
method was used to	9
clinical characteristics of cases	9
the novelty of the	9
coronavirus isolates by human	9
t cells transfected with	9
unexpected receptor functional mimicry	9
rbd in the up	9
for the first time	9
rbd i c regimes	9
of the n k	9
the formation of a	9
cell entry mechanisms of	9
are listed in table	9
with the exception of	9
coronavirus by human monoclonal	9
amino acids in the	9
is defined as the	9
in the general population	9
with the binding of	9
the presence of a	9
syndrome coronavirus by human	9
respiratory syndrome coronavirus by	9
cellular immune response in	9
declare no conflict of	9
the novel human coronavirus	9
the importance of the	9
reactive to the s	9
to s protein that	9
and the supernatant was	9
glycoprotein receptor binding domain	9
spike rbd and block	9
p and s p	9
isolates by human monoclonal	9
the pymol molecular graphics	9
exceptionally potent neutralization of	9
the immune response to	9
and reproduction in any	9
was performed as described	9
the role of the	9
rbd and block interaction	9
of the viral and	9
it should be noted	9
human neutralizing antibody targets	9
functional mimicry elucidates activation	9
receptor functional mimicry elucidates	9
single amino acid substitutions	9
could be used as	9
its interaction with mab	9
complexed with neutralizing antibody	9
page and western blotting	9
prophylactic and therapeutic efficacy	9
of rbd to ace	9
monoclonal antibodies block the	9
at room temperature in	9
structure of severe acute	9
characterization of the receptor	9
prophylactic and postexposure efficacy	9
structure of nl respiratory	9
on virus entry and	9
are included in the	9
virus entry and its	9
these results demonstrate that	9
cells were identified as	9
at the beginning of	9
in the interaction with	9
with its human receptor	9
were obtained from the	9
characterization of spike glycoprotein	9
g a s d	9
and its immune cross	9
in the rbd region	9
reproduction in any medium	9
potent neutralization of middle	9
strong neutralizing antibody responses	9
for subunit vaccine development	9
absorbance at nm was	9
and the ace receptor	9
s protein is a	9
acquired thrombotic thrombocytopenic purpura	9
pymol molecular graphics system	9
see materials and methods	9
human ace structure of	9
neutralizing antibody targets the	9
similar results were obtained	9
the wild type receptor	9
structure of the s	9
was added and incubated	9
infections in engineered human	9
in vero e cells	9
reactive neutralization of sars	9
subunit of the s	9
crystal structure of nl	9
and block interaction with	9
a significant role in	9
specific antibody responses in	9
d g increases infectivity	9
for hour at room	9
plates were washed three	9
elucidates activation of coronavirus	9
respiratory syndrome coronavirus as	9
human monoclonal antibodies against	9
of sars coronavirus isolates	9
the hr domain of	9
epitopes that induce highly	9
performed as described previously	9
these data indicate that	9
a member of the	9
acid substitutions in the	9
binding to the s	9
the cells were incubated	9
antibody targets the receptor	9
alum in a single	9
protein efficiently binds angiotensin	9
of nl respiratory coronavirus	9
spike protein in the	9
that induce highly potent	9
effectiveness of convalescent plasma	9
play an important role	9
mice in each group	9
activation of coronavirus fusion	9
mimicry elucidates activation of	9
grade soluble human ace	9
neutralization of middle east	9
glycosylation sites in the	9
shown in figure c	9
the binding interface of	9
to be the most	9
the beginning of the	9
may be due to	9
a high degree of	9
engineered human tissues using	9
entry and its immune	9
neutralizing nanobodies bind sars	9
the free energy of	9
three times with pbs	9
minutes at room temperature	9
for minutes at room	9
binding to the spike	9
coronavirus middle east respiratory	9
would like to thank	9
evidence that d g	9
hours at room temperature	9
we did not observe	9
a high binding affinity	9
for the neutralization of	9
in the form of	9
on the host cell	9
room temperature for hour	9
rbd vaccine were co	9
at the time of	9
the viral and host	9
the preprint server for	9
between the s subunit	9
cells were incubated for	9
of igg mbcs reactive	9
receptor for sars coronavirus	9
in engineered human tissues	9
basis for the neutralization	9
as a positive control	8
expression and functional characterization	8
each of the three	8
efficacy of a potent	8
s proteins of the	8
party material in this	8
viral load in posterior	8
block interaction with ace	8
in order to determine	8
human mab to s	8
credit line to the	8
ace structure of the	8
was calculated using the	8
added to the wells	8
fold increase in the	8
in the contacting network	8
a potential inhibitor of	8
the dynamics of the	8
will need to obtain	8
of influenza a virus	8
line to the material	8
binding to human ace	8
potential conflict of interest	8
long as you give	8
the interaction of sars	8
antibody responses during infection	8
human igg fc tag	8
angiotensin converting enzyme receptor	8
permission directly from the	8
of the middle east	8
the reaction was stopped	8
to angiotensin converting enzyme	8
structural and functional analysis	8
obtain permission directly from	8
patients define multiple targets	8
mice immunized with the	8
by a human mab	8
american type culture collection	8
indicated otherwise in a	8
the origin of sars	8
the rational design of	8
contacting network to s	8
domain of the s	8
a link to the	8
against spike and rbd	8
emergency of international concern	8
class i viral fusion	8
for rapid unsupervised cryo	8
of a human monoclonal	8
in expi f cells	8
monoclonal antibodies targeting the	8
mediated blockage of ace	8
analyzed by flow cytometry	8
domain of the sars	8
public health emergency of	8
phage display antibody library	8
we were able to	8
due to the lack	8
the treatment of patients	8
the active site of	8
the n r linker	8
patients infected with sars	8
immunological assessment of asymptomatic	8
subjects had igg against	8
serum antibody responses during	8
human immunodeficiency virus type	8
if changes were made	8
collected between april and	8
of the papn ectodomain	8
human monoclonal antibody protects	8
not permitted by statutory	8
intended use is not	8
neutralizing activity against sars	8
a human mab to	8
appropriate credit to the	8
s proteins of sars	8
in good agreement with	8
in a credit line	8
load in posterior oropharyngeal	8
these short ace isoforms	8
and immunological assessment of	8
multiple targets of vulnerability	8
the antigenicity of the	8
changes induced by receptor	8
an important target for	8
statutory regulation or exceeds	8
of the interaction between	8
and middle east respiratory	8
of convalescent plasma and	8
temporal profiles of viral	8
or exceeds the permitted	8
conformation of the s	8
epitopes in the rbd	8
neutralizing antibodies in the	8
clinical and immunological assessment	8
are consistent with the	8
shown in figure s	8
responses during infection by	8
the heavy chain and	8
of coronavirus spike protein	8
peripheral blood mononuclear cells	8
the average of the	8
if material is not	8
indicate if changes were	8
that some other factors	8
permitted by statutory regulation	8
both prophylactic and therapeutic	8
in posterior oropharyngeal saliva	8
the number of activated	8
studies have demonstrated that	8
of the wild type	8
indicating that some other	8
s and n proteins	8
binding to cell surface	8
images or other third	8
compared with human ace	8
the sensitivity of the	8
an exceptionally potent germline	8
comparable to that of	8
at room temperature with	8
to better understand the	8
in the protein data	8
oropharyngeal saliva samples and	8
ill patients with covid	8
syndrome coronavirus spike glycoprotein	8
the performance of the	8
results suggest that the	8
added to the cells	8
administration of convalescent plasma	8
in patients of novel	8
than in unexposed subjects	8
the structure of sars	8
to the receptor binding	8
were used as negative	8
the s s junction	8
the contacting network to	8
sensitivity and specificity of	8
s protein has been	8
structural definition of a	8
had igg against the	8
exceeds the permitted use	8
the human receptor ace	8
related to the sars	8
ace isoforms in these	8
at a flow rate	8
infectivity of the covid	8
s protein that blocks	8
were defined as the	8
respiratory syndrome coronavirus fusion	8
as long as you	8
has been identified as	8
the neutralizing antibody titers	8
a flow rate of	8
neutralizing antibodies against mers	8
that the combination of	8
the main hot spot	8
posterior oropharyngeal saliva samples	8
is in line with	8
your intended use is	8
are critical for mers	8
of its spike protein	8
profiles of viral load	8
s subunit contains the	8
of the viral s	8
health emergency of international	8
conformational change of the	8
into the host cells	8
or other third party	8
neutralization by an exceptionally	8
caused by severe acute	8
nl respiratory coronavirus receptor	8
link to the creative	8
than the intact ace	8
directly from the copyright	8
the rbds of the	8
innate and adaptive immune	8
not adsorbed to alum	8
material is not included	8
third party material in	8
convalescent plasma as a	8
and serum antibody responses	8
were incubated for h	8
protein that blocks receptor	8
in the majority of	8
we also observed that	8
is not permitted by	8
and indicate if changes	8
the critical amino acids	8
use is not permitted	8
antibodies that bind to	8
a humanized neutralizing antibody	8
was carried out by	8
to view a copy	8
serially diluted mouse sera	8
g increases infectivity of	8
length human ace structure	8
any medium or format	8
glycan analysis of the	8
regulation or exceeds the	8
a copy of this	8
the interaction of rbd	8
coronavirus by a human	8
followed by incubation with	8
surrogate virus neutralization test	8
the rbd i c	8
added to the plates	8
unless indicated otherwise in	8
there was no significant	8
to the original author	8
binds to the n	8
md simulations were performed	8
an urgent need for	8
lower than that of	8
cleavage site in the	8
of viral subunit vaccines	8
samples and serum antibody	8
neutralization of severe acute	8
to the lack of	8
neutralization epitope on the	8
domains of the sars	8
potent neutralization of severe	8
respiratory syndrome coronavirus infections	8
wild type or mutant	8
the upper respiratory tract	8
provide a link to	8
combinatorial adjuvant system where	8
and your intended use	8
protein of sars coronavirus	8
material in this article	8
in combination with other	8
in any medium or	8
in convalescent subjects than	8
during infection by sars	8
the top of the	8
can be used to	8
a detailed protocol for	8
article are included in	8
bind to the receptor	8
distribution and reproduction in	8
residues are critical for	8
a credit line to	8
define multiple targets of	8
in the binding of	8
view a copy of	8
increases infectivity of the	8
spike protein and its	8
in the asymmetric unit	8
human monoclonal antibody against	8
is likely to be	8
by surface plasmon resonance	8
were performed in duplicate	8
in supplementary table s	8
cells were seeded at	8
you will need to	8
by an exceptionally potent	8
due to the high	8
interaction between the rbd	8
mab to s protein	8
been shown to be	8
as well as in	8
cleavage at the s	8
patients of novel coronavirus	8
you give appropriate credit	8
the distance between the	8
affinity to human ace	8
give appropriate credit to	8
in this article are	8
complete genome sequence of	8
concentration of mg ml	8
compared to the control	8
the specificity and sensitivity	8
the s subunit contains	8
site on the rbd	8
otherwise in a credit	8
is based on the	8
of this study are	8
need to obtain permission	8
is not included in	8
an observational cohort study	8
other third party material	8
conformational changes induced by	8
algorithms for rapid unsupervised	8
d g mutation in	8
a potential therapy for	8
as described in the	8
used as a control	8
t cells were transfected	8
binding of ace to	8
assessment of asymptomatic sars	8
the spike proteins of	8
fragment molecular orbital method	8
convalescent plasma therapy for	8
saliva samples and serum	8
to obtain permission directly	8
cov neutralization by an	8
that blocks receptor association	8
proximal origin of sars	8
viruses by human monoclonal	8
s protein and ace	8
in the s region	8
credit to the original	8
a highly potent pan	8
plays an important role	8
the neutralizing immunogenicity of	8
a broad range of	8
the functional receptor for	8
interacting with the ace	8
as you give appropriate	8
coronavirus spike proteins in	8
nasal washes and oral	8
weeks after symptom onset	8
to the creative commons	8
the images or other	8
residues in the contacting	8
of s protein and	8
plays an essential role	8
in balb c mice	8
for designing sars vaccines	8
this article are included	8
that the majority of	8
the binding site of	8
spike protein contains multiple	8
area under the curve	8
the inhibitory activity of	8
our understanding of the	8
the proximal origin of	8
from the copyright holder	8
as a consequence of	8
conducted in accordance with	8
to those of the	8
and characterization of a	8
characteristics of cases of	8
have previously shown that	8
by statutory regulation or	8
binding site of the	8
the detection of sars	8
is responsible for binding	7
q t p y	7
as a result of	7
be further improved by	7
and chloroquine effectively inhibit	7
by receptor recognition or	7
and its complex with	7
and convalescent humans yield	7
respiratory distress syndrome in	7
wild type and mutant	7
hr domains of the	7
humoral and cellular immune	7
neutralization determinant of severe	7
with respect to the	7
fusion of viral and	7
and sensitivity of the	7
were then washed with	7
seen with individual antibodies	7
used to calculate the	7
the lower limit of	7
surface of the virus	7
its spike protein that	7
used as a template	7
an essential role in	7
residues on the rbd	7
protocol for a serological	7
of human antibodies bound	7
are resistant to conformational	7
that they have no	7
cs cs cs cs	7
the binding kinetics of	7
dependent neutralizing monoclonal antibody	7
carried out using a	7
of rbd with ace	7
neutralization test based on	7
these findings suggest that	7
were cloned into the	7
mice and convalescent humans	7
with the host receptor	7
i v a v	7
cov with optimal immunogen	7
neutralizing monoclonal antibody specifically	7
overlap with the ace	7
human monoclonal antibodies block	7
and the error bars	7
does not overlap with	7
r and p values	7
were washed times with	7
titers against the s	7
were significantly higher in	7
the last ns of	7
antibody specifically targeting receptor	7
efficacy and safety of	7
protein to angiotensin converting	7
sequence alignment of the	7
identification of a critical	7
at the site of	7
targeting its spike protein	7
this suggests that the	7
induces strong neutralizing antibody	7
declare that they have	7
bound to the rbd	7
on the development of	7
virus neutralization test based	7
a systematic review and	7
immediately upstream of the	7
coronavirus spikes are resistant	7
authors declare that they	7
in published maps and	7
in this study we	7
culture supernatant was collected	7
nm nm nm nm	7
to the viral spike	7
proteins in viral entry	7
composition and divergence of	7
of upward rbd and	7
implication for developing therapeutics	7
the interaction between ns	7
of a potent human	7
a critical neutralization determinant	7
based peptide inhibitors of	7
hek t cells transfected	7
development of an effective	7
from the united states	7
conformational states of sars	7
severe acute respiratory infections	7
incubation at room temperature	7
number of migratory monocytes	7
incubated on ice for	7
the number of migratory	7
developing therapeutics and vaccines	7
studies in humanized mice	7
of the tgev rbd	7
the conserved s subunit	7
targeting the rbd of	7
are likely to be	7
key target for antivirals	7
characterization of a novel	7
antibody cocktail to sars	7
inhibitor targeting its spike	7
access this article is	7
can serve as a	7
were then used to	7
stabilized coronavirus spikes are	7
titers of neutralizing antibodies	7
an equal volume of	7
in the design of	7
binding domain of severe	7
and immunization interval protects	7
viral entry and pathogenesis	7
of the antibody response	7
domain in middle east	7
s and s domains	7
the virus in the	7
that harbors a high	7
one of the three	7
identity with that of	7
the detection of antibodies	7
the supernatant was collected	7
capacity to mediate membrane	7
interface of the rbd	7
responses in coronavirus disease	7
the sensitivity and specificity	7
serial dilutions of the	7
remains neutral with regard	7
cases of novel coronavirus	7
importance for designing sars	7
antibodies bound to sars	7
to be developed as	7
lethal middle east respiratory	7
were carried out with	7
were used for the	7
mutational escape seen with	7
the novel coronavirus disease	7
t cells were seeded	7
high performance molecular simulations	7
structures of human antibodies	7
a salt bridge with	7
the spike protein is	7
in patients with coronavirus	7
amino acid sequence identity	7
analysis of the receptor	7
a consequence of the	7
humanized mice and convalescent	7
interaction between ns and	7
it was reported that	7
comparative protein structure modeling	7
supernatant was collected and	7
the findings of this	7
detailed protocol for a	7
with a plasmid encoding	7
with serial dilutions of	7
to human igg fc	7
critical neutralization determinant of	7
at least of the	7
igg titers against the	7
the expression of the	7
we show that the	7
admitted to the hospital	7
between rbd and ace	7
related viruses by human	7
the interactions between the	7
a creative commons attribution	7
the extracellular domain of	7
neutral with regard to	7
web server for predicting	7
are not susceptible to	7
neutralization activity against sars	7
and host cell membranes	7
in subunit vaccine design	7
influenza a virus ns	7
and induces strong neutralizing	7
the v h ab	7
the host cell surface	7
the s protein binds	7
a concentration of nm	7
d g m i	7
in the number of	7
respiratory syndrome coronavirus and	7
plates were washed with	7
neutralizing antibodies against the	7
we have shown that	7
were identified in the	7
to the rbd and	7
against the s subunit	7
elisa plates were coated	7
remdesivir and chloroquine effectively	7
the interaction with the	7
prevents rapid mutational escape	7
was approved by the	7
the virus to the	7
we would like to	7
harbors a high capacity	7
the recently emerged novel	7
claims in published maps	7
coronavirus pneumonia in wuhan	7
by a novel coronavirus	7
respiratory syndrome coronavirus a	7
antibody responses in covid	7
inhibit the binding of	7
peptide inhibitors of sars	7
of the v h	7
immunization interval protects human	7
of the amino acid	7
spike proteins in viral	7
target for neutralizing antibodies	7
cells were stained with	7
diagnostic accuracy of serological	7
antibodies as well as	7
has the potential to	7
high levels of antibodies	7
cov spike protein and	7
was detected by using	7
h n pdm virus	7
receptor recognition or proteolysis	7
is licensed under a	7
were then added to	7
domain of severe acute	7
in the treatment of	7
identification and characterization of	7
human antibodies bound to	7
caused by the sars	7
chloroquine effectively inhibit the	7
under a creative commons	7
the binding between mers	7
of the viral envelope	7
to the human cell	7
the development of an	7
treatment of critically ill	7
subunit of s protein	7
licensed under a creative	7
of a critical neutralization	7
to block the rbd	7
infection and induces strong	7
serum samples were collected	7
on the rbd of	7
protein potently inhibits mers	7
spike glycoprotein of the	7
a small proportion of	7
viral fusion and entry	7
is thought to be	7
immune responses against sars	7
data are shown as	7
national institutes of health	7
the neutralization of sars	7
viral and host cell	7
cov spike protein potently	7
with the rbd in	7
listed in table s	7
interaction with the human	7
induced by receptor recognition	7
of hospitalized patients with	7
affect the interaction of	7
the n k variant	7
at room temperature and	7
were added to each	7
coronavirus in patients with	7
in intensive care units	7
of the protein was	7
of the binding interface	7
by a factor of	7
of type i ifn	7
of each of the	7
protein prevents rapid mutational	7
recently emerged novel coronavirus	7
bind to rbd in	7
be explained by the	7
escape seen with individual	7
of the af mab	7
is a receptor for	7
for developing therapeutics and	7
and severe acute respiratory	7
protein of the hcov	7
as shown in the	7
b and t cell	7
a function of the	7
of the rbd in	7
the expression of ace	7
convalescent humans yield a	7
plates were incubated with	7
and protective efficacy against	7
our results showed that	7
the amino acid sequence	7
the proximal promoters of	7
this indicates that the	7
domain of sars coronavirus	7
a public health emergency	7
novel coronavirus pneumonia in	7
a potent human monoclonal	7
cause of severe acute	7
spike proteins of sars	7
protein that harbors a	7
performance molecular simulations through	7
binding domain in middle	7
immunogen dosage and immunization	7
regard to jurisdictional claims	7
epidemiological and clinical characteristics	7
a major target of	7
to mediate membrane fusion	7
potential therapy for covid	7
induces highly potent neutralizing	7
to jurisdictional claims in	7
this article is licensed	7
and the number of	7
our results indicate that	7
cov infection and induces	7
spike protein that harbors	7
is an important target	7
broad neutralization of sars	7
in at least of	7
performed in duplicate and	7
spike protein prevents rapid	7
with regard to jurisdictional	7
absorbance was measured at	7
and incubated overnight at	7
spike protein potently inhibits	7
structure of the receptor	7
domain antibodies against sars	7
to determine if the	7
development of neutralizing antibodies	7
single amino acid substitution	7
of neutralizing antibodies and	7
and hr domains of	7
of the new coronavirus	7
by a highly potent	7
of the s and	7
high capacity to mediate	7
coronaviruses in malayan pangolins	7
potential inhibitor of sars	7
the novel coronavirus sars	7
closely related to sars	7
were observed in the	7
a high capacity to	7
to conformational changes induced	7
nature remains neutral with	7
structure analysis of the	7
associated with acute respiratory	7
antibodies block the binding	7
responsible for receptor binding	7
deposited in the protein	7
with optimal immunogen dosage	7
play a role in	7
p values are indicated	7
of human immunodeficiency virus	7
the binding of ace	7
inhibitor targeting the hr	7
expression in mammalian cells	7
expressed on the surface	7
from laptops to supercomputers	7
for at least days	7
in an animal model	7
patients with middle east	7
a protein concentration of	7
the ace receptor structural	7
the neutralizing antibody response	7
were performed as described	7
antibody responses in coronavirus	7
of the rbd with	7
dosage and immunization interval	7
were washed with pbs	7
the plate was washed	7
cells were then washed	7
in the middle east	7
rapid mutational escape seen	7
expressed norovirus shell domain	7
fusion inhibitor targeting its	7
s region of the	7
spikes are resistant to	7
determinant of severe acute	7
fusion and entry into	7
containing fetal bovine serum	7
free energy of binding	7
data suggest that the	7
the virus and the	7
new insights into the	7
used to determine the	7
viral and cellular membranes	7
in complex with human	7
the host immune system	7
as a potential drug	7
and purified by ni	7
between s and s	7
the prefusion conformation structural	7
were predicted to be	7
we are grateful to	7
postexposure efficacy of a	7
humans yield a sars	7
cell culture supernatant was	7
well and incubated at	7
is closely related to	7
resistant to conformational changes	7
generation and characterization of	7
in the lungs of	7
spike protein to angiotensin	7
inhibit the recently emerged	7
structural analysis of the	7
maps and institutional affiliations	7
were coated overnight at	7
absence or presence of	7
monoclonal antibody specifically targeting	7
to the s and	7
against the rbd of	7
as a receptor for	7
of human monoclonal antibodies	7
cov spike protein with	7
and other human coronaviruses	7
immune response in mice	7
and entry into host	7
patients in hong kong	7
residues in the s	7
the online version of	7
the course of the	7
by the fact that	7
parallelism from laptops to	7
the results of the	7
interaction between rbd and	7
a k d of	7
antibody against mers coronavirus	7
analysis of the spike	7
the s protein has	7
type or mutant mers	7
in humanized mice and	7
upper and lower respiratory	7
in viral entry and	7
neutralizing activity of the	7
specific glycan analysis of	7
test based on antibody	7
significantly higher in mice	7
human and camel mers	7
article is licensed under	7
the angiotensin converting enzyme	7
was determined using the	7
for a serological assay	7
of convalescent plasma for	7
open access this article	7
springer nature remains neutral	7
the presence of ace	7
in major livestock species	7
supplemented with fetal bovine	7
of s and s	7
findings of this study	7
jurisdictional claims in published	7
respiratory syndrome coronavirus entry	7
genomic characterization of the	7
was performed using a	7
effectively inhibit the recently	7
had no effect on	7
related coronaviruses in malayan	7
of ace genes in	7
this is the first	7
a competitive biopanning strategy	7
in line with the	7
hcovs oc and e	7
the fc region of	7
optimal immunogen dosage and	7
binding affinity of protein	7
potent human monoclonal antibody	7
s s and s	7
published maps and institutional	7
have the potential to	7
speed and accuracy of	6
structure of the complex	6
expressed in expi cells	6
by the use of	6
in the npt ensemble	6
when compared to the	6
the national institutes of	6
novel coronavirus outbreak of	6
rbd a c or	6
structural insights into coronavirus	6
the s subunit mediates	6
on the random coils	6
first step in understanding	6
the mixture was then	6
and the plates were	6
a novel nanobody targeting	6
or rbd i c	6
as a potential therapy	6
was immobilized on the	6
the cell entry of	6
specific memory b cells	6
can bind to the	6
within the s domain	6
we also found that	6
severe acute lung failure	6
g m i d	6
higher in convalescent subjects	6
figure a and b	6
preprint server for health	6
epitopes and recurrent features	6
the reciprocal of the	6
treatment of patients with	6
our data show that	6
the design of the	6
antibodies targeting the rbd	6
the impact of the	6
correlations were performed and	6
duplicate and the error	6
of viruses related to	6
and s subunits of	6
tissue culture infectious dose	6
data indicate that the	6
masked with kapton tape	6
terminal human igg fc	6
and immunogenicity of a	6
of the same clade	6
proteins h and ttd	6
binding domain elicits a	6
the influenza a virus	6
antibodies against the rbd	6
tnts were synthesized by	6
for viral fusion and	6
target for the development	6
and cellular immune responses	6
the presence of nm	6
cov of the same	6
to a density of	6
from the zinc database	6
and entry into the	6
in direct contact with	6
under positive selection in	6
spike protein of severe	6
in cov of the	6
the contribution of the	6
this study did not	6
m i d g	6
highly pathogenic avian influenza	6
based on the rbd	6
the ace receptor binding	6
lymph node targeting and	6
reveal common epitopes and	6
and r and p	6
arabia middle east respiratory	6
nanobody targeting middle east	6
development of an inactivated	6
the affinity of the	6
receptor recognition mechanisms of	6
n and h n	6
distortion on the classical	6
we find that the	6
may be used as	6
and recurrent features of	6
spike glycoprotein and its	6
was no significant difference	6
pathogenic coronavirus isolated from	6
were selected based on	6
a molar ratio of	6
in human airway epithelial	6
while the s subunit	6
of the clinical and	6
monoclonal antibodies against highly	6
the interaction between rbd	6
s protein binding to	6
the stabilization of the	6
acid residues in the	6
in this study are	6
in golden syrian hamster	6
for neutralization of sars	6
em structure of sars	6
were performed and r	6
it is necessary to	6
for predicting the binding	6
implications for disease pathogenesis	6
with the s subunit	6
specific igg and igm	6
epitopes within the rbd	6
room temperature in the	6
attachment to sialic acid	6
performed to determine the	6
was performed to determine	6
was also observed in	6
the early stage of	6
that the magnitude of	6
g mutation in the	6
predicting the binding affinity	6
plasma as a potential	6
vaccines and therapeutic antibodies	6
the s domain is	6
this paper at https	6
the neutralization activity of	6
effect of the d	6
accuracy of docking with	6
binding of the viral	6
version of this article	6
could also have been	6
proven protease inhibitor structure	6
s subunit and rbd	6
in mice immunized with	6
supplementary information is available	6
docking with a new	6
each of the four	6
context of the full	6
humoral immune responses against	6
in dmem supplemented with	6
was defined as the	6
followed by the addition	6
of potential vaccine targets	6
vaccine targets for the	6
transition of the s	6
of viral and host	6
the interface between the	6
the innate immune response	6
patient with atypical pneumonia	6
to severe acute respiratory	6
and p values are	6
pathological manifestations of coronavirus	6
human monoclonal antibody that	6
an ec value of	6
is supported by the	6
our results suggest that	6
the expression levels of	6
information is available at	6
t cell responses to	6
distribution of ace protein	6
highly conserved hr and	6
to sialic acid receptors	6
the evolution of the	6
that there was no	6
intermediate hosts of sars	6
with serially diluted mouse	6
was read at nm	6
of the rbd surface	6
crystal structure of human	6
in a humidified atmosphere	6
infection of human lung	6
human ace structural basis	6
been identified as a	6
of vaccines and therapeutic	6
safety and immunogenicity of	6
for the binding to	6
it is interesting to	6
in the common marmoset	6
the creative commons license	6
in the closed conformation	6
domain has potent cross	6
cleared by centrifugation at	6
and s ace blocking	6
in mice where rasp	6
biotherapeutics for lung diseases	6
the amino acid pairs	6
a new scoring function	6
the plate was incubated	6
immunopathology on challenge with	6
transgenic mice expressing human	6
was higher than that	6
to compete with ace	6
predict candidate targets for	6
binding sites on the	6
the etiological agent of	6
deep mutational scanning of	6
the immunogenicity of rbd	6
a potent inhibitor of	6
for the electrochemical detection	6
and functional analysis of	6
van der waals interactions	6
s protein and antibodies	6
genomic characterization of a	6
of the hcov oc	6
outbreak of middle east	6
and similar results were	6
of mbcs reactive to	6
studies are needed to	6
commons license and your	6
against severe acute respiratory	6
the hr and hr	6
with the receptor ace	6
to rbd and s	6
the anodization was carried	6
the binding epitope of	6
in duplicate and the	6
mixtures were added to	6
e d y q	6
based subunit vaccines against	6
and pathological manifestations of	6
of the ace orthologs	6
the rbd region of	6
index to the rational	6
structure was solved by	6
the residues involved in	6
form of the sars	6
creative commons license and	6
the size of the	6
igg and iga antibodies	6
human transgenic mice from	6
for any of the	6
potential vaccine targets for	6
were extracted from the	6
could be used to	6
the outer surface of	6
study are available from	6
from a patient with	6
functional distortion on the	6
n k g d	6
atypical pneumonia after visiting	6
unexposed subjects that could	6
manifestations of coronavirus disease	6
of the s ectodomain	6
k g d r	6
classical long ace isoforms	6
after the first round	6
to evaluate the efficacy	6
domain in the s	6
cov spike protein are	6
old female balb c	6
of mice that received	6
can be blocked by	6
the first round of	6
computational design of ace	6
from the standard biopanning	6
was measured using an	6
step electrochemical anodization route	6
protein induces highly potent	6
t i v a	6
bind simultaneously to the	6
and treatment of covid	6
also have been performed	6
server for predicting the	6
the diagnostic accuracy of	6
t test was performed	6
due to the presence	6
for the receptor binding	6
isolated from a patient	6
the fc domain of	6
balb c mice were	6
who have recovered from	6
and its inhibition by	6
were expressed in expi	6
for prophylaxis and therapy	6
bind to the sars	6
angiotensin i to angiotensin	6
coronavirus attachment to sialic	6
interactions between the sars	6
the g d mutation	6
cells were grown in	6
cells were cultured in	6
isoforms in these animal	6
expressed in hek t	6
of igm and iga	6
at the university of	6
a potential drug target	6
of an effective vaccine	6
by molecular replacement using	6
rbd domain of sars	6
dependent epitopes that induce	6
cell response to sars	6
immunodominant sites on the	6
the side chains of	6
the error bars denote	6
based on the receptor	6
over the course of	6
newly emerged middle east	6
and cellular immunity in	6
entry into the cells	6
d g mutation of	6
referred to as the	6
cells were washed with	6
decade of structural studies	6
i and type iii	6
culture supernatants were collected	6
golden syrian hamster model	6
from severe acute lung	6
at a molar ratio	6
should be addressed to	6
a severe acute respiratory	6
with inactivated h n	6
supernatant was transferred to	6
of a unique neutralization	6
respiratory syndrome coronavirus to	6
microscopy structure of a	6
mouse model of mers	6
inhibitors of severe acute	6
using open source software	6
days or more after	6
levels and neutralization titers	6
associated with disease severity	6
molecular simulations through multi	6
with atypical pneumonia after	6
using the crystal structure	6
mice were immunized with	6
were incubated at room	6
the development of neutralizing	6
attachment to host cells	6
the data are presented	6
license and your intended	6
were calculated using the	6
antibody binding to the	6
days after the onset	6
has been used to	6
antibodies against highly conserved	6
protects human transgenic mice	6
and then incubated with	6
site absent in cov	6
viral and host membranes	6
that the rbd domain	6
syndrome coronavirus fusion inhibitor	6
in the rbd domain	6
the absence or presence	6
and the s protein	6
both pseudovirus and live	6
are more broadly neutralizing	6
a patient with atypical	6
neutralizing activity and protective	6
at a density of	6
virus by recombinant ace	6
receptorbinding domain complexed with	6
isoforms in domestic animals	6
design of mers coronavirus	6
subjects than in unexposed	6
of the human ace	6
the activation of the	6
receptor binding site of	6
type i and type	6
a unique neutralization epitope	6
did not observe any	6
of isre stat and	6
d g i v	6
in these animal species	6
recurrent features of antibodies	6
of the mutant rbds	6
binding to the sars	6
rational design of mers	6
neutralizing antibody titers and	6
have shown that the	6
emerged middle east respiratory	6
is highly conserved among	6
syndrome coronavirus as an	6
conformational changes of the	6
of convalescent subjects had	6
disease pathogenesis and transmissibility	6
spike glycoprotein and the	6
to be susceptible to	6
of docking with a	6
in the spike gene	6
humoral immune response to	6
tissue distribution of ace	6
the secondary structure of	6
rapid generation of a	6
critical amino acids and	6
domain elicits a potent	6
coronavirus spike protein and	6
for the s ligand	6
we were interested in	6
the prefusion conformation a	6
prophylaxis and therapy of	6
the evolution of sars	6
experiments were carried out	6
engagement and functional distortion	6
washes and oral swabs	6
respiratory syndrome coronavirus challenge	6
open source software such	6
similar to those of	6
as inhaled biotherapeutics for	6
potent neutralizing response without	6
in the same way	6
cannot be ruled out	6
to the rational design	6
in two animal models	6
summarized in supplementary table	6
plates were then washed	6
adjuvant system where rasp	6
protein are more broadly	6
terminal end of the	6
for hours at room	6
attachment of the virus	6
of mab h to	6
could serve as a	6
neutralizing antibodies in mice	6
pearson correlations were performed	6
the conformational change of	6
for receptor binding and	6
the overall flexibility of	6
vero e cells and	6
h n and h	6
the s trimer with	6
of global health concern	6
targeting the receptor binding	6
binding domain has potent	6
coronavirus outbreak of global	6
on the classical long	6
after the last immunization	6
hydrogen bond with rbd	6
inhaled biotherapeutics for lung	6
this analysis could also	6
was used to perform	6
trimer in complex with	6
the creative commons attribution	6
human coronavirus attachment to	6
absent in cov of	6
for disease pathogenesis and	6
the sequences of the	6
targeting middle east respiratory	6
performed using open source	6
simulation of the clinical	6
cov relative to cov	6
immunized with rbd rasp	6
based design of prefusion	6
blocking the binding of	6
is available in the	6
these results showed that	6
been performed using open	6
spike reveal common epitopes	6
the speed and accuracy	6
cr and ey a	6
the ace binding interface	6
between the human ace	6
neutralizing monoclonal antibodies targeting	6
spike protein are more	6
purified by protein a	6
model for middle east	6
should be directed to	6
with the help of	6
for human coronavirus attachment	6
the cells were washed	6
at the plasma membrane	6
protein contains multiple conformation	6
novel coronavirus in patients	6
of the creative commons	6
insights into coronavirus entry	6
rat ace to an	6
enrichment of isre stat	6
intranasal delivery of sars	6
the viral life cycle	6
coronavirus isolated from a	6
neutralizing antibody titers in	6
i viral fusion protein	6
from a convalescent patient	6
preliminary identification of potential	6
a stable cho cell	6
at least two independent	6
have been reported to	6
the absence of any	6
ace receptor binding site	6
prefusion conformation structural basis	6
treatment of severe acute	6
of the neutralizing antibody	6
regions of the s	6
activity and protective efficacy	6
the short ace isoforms	6
control proteins h and	6
the attachment of the	6
play a significant role	6
a major target for	6
a virus ns protein	6
an incipient wetting method	6
identification of potential vaccine	6
inhibition by neutralizing antibodies	6
mutations in the spike	6
source data are provided	6
detection of novel coronavirus	6
common epitopes and recurrent	6
interaction with the host	6
step in understanding sars	6
pneumonia after visiting wuhan	6
with a new scoring	6
analysis could also have	6
added to the plate	6
and fusion of the	6
compared to the rbd	6
mutations in the rbd	6
heavy chain variable domain	6
at the active site	6
targets for immune responses	6
major target of neutralizing	6
purchased from sino biological	6
a potent neutralizing response	6
for immune responses to	6
entry into the cell	6
with the novel coronavirus	6
figure b and c	6
evaluate the neutralizing immunogenicity	6
there was a significant	6
in the wild type	6
igg binding to the	6
against highly conserved hr	6
the early stages of	6
structural basis for human	6
of antibodies against sars	6
plays a key role	6
a decade of structural	6
recognition mechanisms of coronaviruses	6
novel nanobody targeting middle	6
of respiratory syncytial virus	6
therapy in sars patients	6
the ln of mice	6
important role in the	6
level parallelism from laptops	6
pseudovirus and live mers	6
corresponding to the s	6
improving the speed and	6
its inhibition by neutralizing	6
spike protein has a	6
mechanism of action of	6
mutation in the sars	6
from the corresponding author	6
baked in an oven	6
potential to be developed	6
by the viral s	6
not overlap with the	6
in spike protein of	6
hrv c d g	6
structural characterization of the	6
the expression of a	6
cleavage site absent in	6
a first step in	6
of antibody responses in	6
to the rbd in	6
block the interaction between	6
number of activated monocytes	6
with convalescent plasma for	6
with different concentrations of	6
glycoprotein of the new	6
on the cell surface	6
to cell surface papn	6
and accuracy of docking	6
performed and r and	6
deep mutational scan of	6
the strength of the	6
a novel coronavirus in	6
of novel coronavirus pneumonia	6
basis for human coronavirus	6
amino acid sequence of	6
block the entry of	6
the impact of mutations	6
characterization of the novel	6
binding interface of the	6
ncov contains a furin	6
a c or rbd	6
at the top of	6
design and development of	6
and rbd of sars	6
dissociation constant k d	6
at the interface between	6
was first detected in	6
can predict candidate targets	6
the s protein trimer	6
compared to the sars	6
from the ace binding	6
that interact with the	6
clinical and pathological manifestations	6
was performed with the	6
definition of a unique	6
rounds of phage display	6
for the binding of	6
cov s protein and	6
elisa was performed to	6
the clinical and pathological	6
with disease severity in	6
complex with human ace	6
fusion inhibitor targeting the	6
to the active site	6
candidate targets for immune	6
in unexposed subjects that	6
of cases of novel	6
to the sars coronavirus	6
tfh and gc b	6