quadgram

This is a table of type quadgram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

quadgram	frequency
porcine reproductive and respiratory	2492
reproductive and respiratory syndrome	2483
and respiratory syndrome virus	2129
of porcine reproductive and	918
the porcine reproductive and	152
respiratory syndrome virus infection	137
respiratory syndrome virus in	137
to porcine reproductive and	132
with porcine reproductive and	125
in the presence of	109
as shown in fig	108
pathogenic porcine reproductive and	83
highly pathogenic porcine reproductive	82
in porcine reproductive and	81
of the porcine reproductive	77
in the present study	67
cells were infected with	66
and respiratory syndrome viruses	66
were infected with prrsv	66
infertility and respiratory syndrome	65
swine infertility and respiratory	65
infected with porcine reproductive	63
in the absence of	63
respiratory syndrome virus and	61
in the united states	60
has been shown to	60
at an moi of	58
strains of porcine reproductive	57
porcine epidemic diarrhea virus	57
an important role in	57
severe acute respiratory syndrome	55
the end of the	55
against porcine reproductive and	55
used in this study	54
in the lungs of	54
respiratory syndrome virus by	53
of swine infertility and	53
the replication of prrsv	52
cells were transfected with	52
a member of the	50
replication of porcine reproductive	50
for porcine reproductive and	49
by porcine reproductive and	49
syndrome virus infection in	48
on the other hand	47
in pigs infected with	46
at a concentration of	44
for the detection of	43
one of the most	42
type porcine reproductive and	41
respiratory syndrome virus replication	40
protein of porcine reproductive	40
strain of porcine reproductive	40
incubated for h at	40
simian hemorrhagic fever virus	39
rna was extracted from	39
respiratory syndrome virus isolates	38
total rna was extracted	38
the economic impact of	37
in the current study	37
the antiviral activity of	37
isolates of porcine reproductive	36
pigs infected with prrsv	35
the results showed that	35
is one of the	35
acute respiratory syndrome coronavirus	35
cells were treated with	35
virus porcine reproductive and	35
been shown to be	35
respiratory syndrome virus nucleocapsid	35
syndrome virus nucleocapsid protein	35
assessment of the economic	34
play an important role	34
impact of porcine reproductive	34
innate and adaptive immune	34
of the economic impact	34
of equine arteritis virus	33
and simian hemorrhagic fever	33
detection of porcine reproductive	33
respiratory syndrome virus gp	33
economic impact of porcine	33
porcine epidemic abortion and	32
and porcine reproductive and	32
abortion and respiratory syndrome	32
in the case of	32
epidemic abortion and respiratory	32
respiratory syndrome virus the	32
proteins of porcine reproductive	31
washed three times with	31
studies have shown that	31
as shown in figure	31
was used as a	31
peripheral blood mononuclear cells	31
gp and m proteins	31
at the time of	30
pcd and vero cells	30
european porcine reproductive and	30
porcine respiratory disease complex	30
in vitro and in	30
the cells were washed	29
respiratory syndrome virus vaccine	29
of the immune response	29
the causative agent of	29
presence or absence of	29
the lungs of pigs	29
the pi k akt	29
north american and european	29
for the presence of	28
the cells were then	28
the presence of prrsv	28
infected with prrsv at	28
the host immune response	28
orf a and orf	28
have been shown to	28
swine disease in the	27
in the development of	27
vitro and in vivo	27
disease in the netherlands	27
live porcine reproductive and	27
mystery swine disease in	27
respiratory syndrome virus nonstructural	27
and vero cells were	27
syndrome virus nonstructural protein	26
as well as the	26
the swine industry worldwide	26
isolation of lelystad virus	26
a and orf b	26
the innate immune response	26
of porcine circovirus type	26
characterization of swine infertility	26
the isolation of lelystad	26
pi k akt pathway	26
of pigs infected with	26
type i ifn signaling	26
for the development of	26
pigs infected with porcine	26
the presence or absence	26
play a role in	25
of the viral genome	25
with fetal bovine serum	25
in porcine alveolar macrophages	25
at the end of	25
important role in the	25
is a member of	25
min at room temperature	25
production of proinflammatory cytokines	25
was found to be	25
were found to be	25
pigs exposed to prrsv	25
respiratory syndrome virus porcine	24
was observed in the	24
van reeth et al	24
den boon et al	24
h n influenza virus	24
response to porcine reproductive	24
for min at room	24
classical swine fever virus	23
replication of prrsv in	23
of prrsv and pedv	23
plays an important role	23
immune response to prrsv	23
responses to porcine reproductive	23
porcine respiratory and reproductive	23
respiratory syndrome virus on	23
the unfolded protein response	23
pathogenesis of porcine reproductive	22
of the north american	22
infection of pigs with	22
in the infected pigs	22
in the respiratory tract	22
it is possible that	22
in the context of	22
cells were maintained in	22
isolate of porcine reproductive	22
syndrome virus porcine reproductive	22
gp of porcine reproductive	22
the cells were infected	22
the adaptive immune response	22
of severe acute respiratory	22
enhanced replication of porcine	22
the immune response to	22
in the presence or	22
to that of the	22
it has been shown	22
us porcine reproductive and	22
at the same time	22
van dinten et al	22
outbreaks of atypical prrs	21
unparalleled outbreaks of atypical	21
on swine production in	21
and incubated for h	21
with that of the	21
and respiratory syndrome on	21
and respiratory syndrome in	21
swine production in the	21
syndrome on swine production	21
of highly pathogenic porcine	21
innate and adaptive immunity	21
respiratory syndrome virus strain	21
was used as the	21
of porcine epidemic abortion	21
production in the united	21
were washed three times	21
respiratory syndrome virus comparison	21
respiratory and reproductive syndrome	21
respiratory syndrome on swine	21
the molecular biology of	21
and adaptive immune responses	21
a homogeneous subpopulation of	20
the formation of autophagosomes	20
virus in a homogeneous	20
of the family arteriviridae	20
homogeneous subpopulation of ma	20
respiratory syndrome virus nsp	20
cells were incubated with	20
proteins encoded by orfs	20
a key role in	20
infection with porcine reproductive	20
of atypical prrs in	20
similar to that of	20
the humoral immune response	20
has been shown that	20
molecular dissection of the	20
in a homogeneous subpopulation	20
respiratory syndrome virus strains	20
of fatal prrsv variants	20
and the cells were	20
emergence of fatal prrsv	20
dissection of the unique	20
atypical prrs in china	20
pigs persistently infected with	20
be due to the	20
of the unique hallmark	20
results showed that the	20
the prrsv n protein	20
to the family arteriviridae	19
china and molecular dissection	19
prrs in china and	19
the expression of il	19
and experimental reproduction of	19
a nested set of	19
as a negative control	19
by the use of	19
experimental reproduction of the	19
of the disease in	19
in china and molecular	19
of type i ifn	19
a multiplicity of infection	19
causative agent of porcine	19
were incubated for h	19
reproduction of the disease	19
for h at room	19
in the lungs and	19
the cells were incubated	19
genomic rna and sg	19
and molecular dissection of	19
the authors declare that	19
in the serum of	18
the replication of porcine	18
new order comprising coronaviridae	18
was added to the	18
h at room temperature	18
as a result of	18
order comprising coronaviridae and	18
of swine to porcine	18
prrsv infection in pigs	18
in the number of	18
in cells infected with	18
respiratory syndrome virus vaccines	18
for h at c	18
of the most important	18
cells were washed with	18
of porcine alveolar macrophages	18
was detected in the	18
in pigs exposed to	18
cells were washed twice	18
a new order comprising	18
the dub activity of	18
in north america and	18
reproductive failure in sows	18
immunity to porcine reproductive	18
swine to porcine reproductive	18
for h and then	18
in this study were	18
respiratory syndrome virus date	18
the presence of the	18
pigs porcine reproductive and	18
supplemented with fetal bovine	17
shown in figure a	17
the disease in gnotobiotic	17
were expressed as the	17
evolution on two continents	17
well tissue culture plates	17
a critical role in	17
the prrsv e protein	17
america and experimental reproduction	17
cells were inoculated with	17
and porcine circovirus type	17
divergent evolution on two	17
at a multiplicity of	17
prrsv at an moi	17
by the addition of	17
in the order nidovirales	17
syndrome virus and porcine	17
animal care and use	17
isolation of swine infertility	17
comprising coronaviridae and arteriviridae	17
the expression of ifn	17
disease in gnotobiotic pigs	17
we found that the	17
differential production of proinflammatory	17
rna and sg mrna	17
proteolytic processing of the	17
were transfected with the	17
the expression levels of	17
of proteins encoded by	17
vivo and in vitro	17
north america and experimental	17
to the swine industry	17
in the porcine reproductive	17
previous studies have shown	16
in cells treated with	16
an infectious cdna clone	16
of virus replication in	16
inhibits porcine reproductive and	16
of pigs persistently infected	16
susceptibility to porcine reproductive	16
respiratory syndrome virus encoded	16
the crystal structure of	16
a better understanding of	16
with prrsv at an	16
african green monkey kidney	16
or infected with prrsv	16
the expression of the	16
respiratory disease in pigs	16
humoral immune response to	16
tissue culture infectious dose	16
infected with prrsv alone	16
host innate immune response	16
similar to those of	16
and reproductive syndrome virus	16
in vivo and in	16
respiratory syndrome virus characterization	15
was added to each	15
of the lelystad virus	15
the detection of porcine	15
syndrome virus characterization of	15
the structural proteins of	15
authors declare that they	15
immune responses to porcine	15
these results indicate that	15
to ldv and eav	15
member of the family	15
modified live porcine reproductive	15
of the most economically	15
is related to ldv	15
declare that they have	15
prrsv nsp and nsp	15
these results indicated that	15
added to each well	15
that they have no	15
the north american and	15
were considered statistically significant	15
porcine respiratory coronavirus in	15
compared to pigs infected	15
infected or infected with	15
were detected in the	15
infected with prrsv and	15
molecular biology of arteriviruses	15
a large number of	15
human immunodeficiency virus type	15
these results suggest that	15
of the prrsv genome	15
were washed twice with	15
the development of a	15
plates were infected with	15
a highly pathogenic porcine	15
inhibition of porcine reproductive	15
are summarized in table	15
of influenza a virus	15
encoded by orfs to	15
by orfs to of	15
pigs experimentally infected with	15
the viral life cycle	15
van marle et al	15
care and use committee	15
agent of porcine epidemic	15
gp and gp proteins	15
respiratory syndrome virus is	15
related to ldv and	15
and then infected with	15
of neutralizing antibodies in	15
pigs with porcine reproductive	15
lower than that of	15
syndrome virus in pigs	15
to pigs infected with	15
evolution of porcine reproductive	15
syndrome virus encoded by	15
of the virus in	15
the development of the	15
analysis of porcine reproductive	14
the cysteine protease domain	14
cells were harvested and	14
syndrome virus in the	14
syndrome virus isolates with	14
prrsv replication in marc	14
host innate immune responses	14
at the indicated times	14
in the cytoplasm of	14
is not required for	14
of the virus to	14
economic losses to the	14
of viral rna in	14
syndrome virus replication in	14
cells were grown in	14
response to swine arterivirus	14
antiviral activity against prrsv	14
pp a and pp	14
is essential for virus	14
virus isolates with that	14
that of the lelystad	14
for the expression of	14
was used to detect	14
of the infected pigs	14
on the surface of	14
genotype of porcine reproductive	14
belongs to the family	14
of the nucleocapsid protein	14
respiratory syndrome virus induces	14
in accordance with the	14
the atpase activity of	14
in the lipid rafts	14
the host response to	14
in the swine industry	14
medium was replaced with	14
isolates with that of	14
immune responses in pigs	14
a significant increase in	14
the mrna level of	14
of the adaptive immune	14
was performed as described	14
of proinflammatory cytokines in	14
in the induction of	14
atpase activity of prrsv	14
with the porcine reproductive	14
of type i ifns	14
with the immune system	14
structural proteins of the	14
in the frequency of	13
the severity of the	13
data are presented as	13
cells were cultured in	13
immune response in pigs	13
challenge of prrs immunology	13
the ability of the	13
pigs infected with vr	13
the inhibitory effect of	13
was not detected in	13
of the efficacy of	13
of the immune system	13
little is known about	13
cells were washed three	13
a modified live porcine	13
during the course of	13
the culture medium was	13
on the cell surface	13
infection and proportion of	13
it has been reported	13
this is the first	13
the pathogenicity of two	13
the most economically important	13
north american strains of	13
of prrsv in marc	13
g for min at	13
proinflammatory cytokines in the	13
the largest rna virus	13
better understanding of the	13
thai medicinal plant extracts	13
the genetic diversity of	13
three times with pbs	13
of differentiation and activation	13
may be due to	13
was performed according to	13
by interfering with the	13
the total number of	13
of the arterivirus group	13
natural science foundation of	13
it has been demonstrated	13
with bovine serum albumin	13
in a humidified co	13
duration of infection and	13
failure in sows and	13
epidemiology of porcine reproductive	13
innate immune response by	13
a wide range of	13
the challenge of prrs	13
the cell lysates were	13
has been reported that	13
a and pp ab	13
cells were fixed with	13
have been identified in	13
are involved in the	13
activity of prrsv helicase	13
well plates were infected	13
and proportion of pigs	13
the role of the	13
of the arterivirus replicase	13
and the number of	13
to the development of	13
and activation of monocytes	13
there was no significant	13
has been reported to	13
of infection and proportion	13
persistently infected with porcine	13
the early stage of	13
van gorp et al	13
work was supported by	13
current knowledge on the	13
of the orf gene	13
this work was supported	13
genes of porcine reproductive	13
were significantly lower in	13
the innate and adaptive	13
proportion of pigs persistently	13
lymphoid tissues of pigs	12
cells were seeded in	12
respiratory syndrome virus emerging	12
p r o o	12
the cells were lysed	12
assay was performed as	12
humoral and cellular immune	12
and interaction with the	12
was supported by the	12
pathogenesis and interaction with	12
virus on united states	12
pig lung during different	12
concentration of mg ml	12
detection and differentiation of	12
arterivirus molecular biology and	12
of p and p	12
activation of monocytes macrophages	12
l p r e	12
the present study was	12
interaction with the immune	12
performed as described previously	12
on their susceptibility to	12
during different respiratory virus	12
identification of porcine reproductive	12
the nucleocapsid protein of	12
blood samples were collected	12
in the pathogenesis of	12
from porcine reproductive and	12
to porcine respiratory coronavirus	12
nsp and nsp are	12
r o o f	12
comparison of the pathogenicity	12
a humidified co incubator	12
the presence of sqe	12
respiratory syndrome virus rna	12
at different time points	12
american and european isolates	12
the pig lung during	12
o u r n	12
our results showed that	12
european and north american	12
and approved the final	12
we found that prrsv	12
u r n a	12
this study was to	12
syndrome virus gp ectodomain	12
n a l p	12
is located in the	12
molecular biology and pathogenesis	12
antibody repertoire development in	12
in contrast to the	12
the formation of the	12
the innate immune system	12
innate immunity and porcine	12
of the gdd motif	12
cytokines in the pig	12
r n a l	12
the immune response of	12
of a highly pathogenic	12
the severe acute respiratory	12
virus infection in neonatal	12
american porcine reproductive and	12
be involved in the	12
of the pathogenicity of	12
the equine arteritis virus	12
mutational analysis of the	12
alpha response to swine	12
at each time point	12
virus belonging to the	12
characterization of porcine reproductive	12
syndrome virus on united	12
was performed using the	12
in the late s	12
virus emerging in china	12
after incubation for h	12
immunity and porcine reproductive	12
be related to the	12
of the present study	12
of this study was	12
european genotype of porcine	12
authors read and approved	12
united states pork producers	12
followed by cycles of	12
type i interferon production	12
h prior to infection	12
differentiation and activation of	12
largest rna virus genome	12
different respiratory virus infections	12
essential for virus infectivity	12
for an additional h	12
in the lung of	12
lung during different respiratory	12
a l p r	12
and the expression of	12
north american porcine reproductive	12
with a potent adjuvant	12
for h prior to	12
pigs of all ages	12
and european isolates of	12
polyclonal b cell activation	12
an infectious clone of	12
of two us porcine	12
infection in neonatal pigs	12
two us porcine reproductive	12
high morbidity and mortality	12
on the replication of	12
respiratory coronavirus in pigs	12
the infected cells were	12
j o u r	12
are listed in table	12
syndrome virus emerging in	12
infectious cdna clone of	12
inactivated porcine reproductive and	12
respiratory syndrome virus through	12
inhibit the replication of	12
in the regulation of	12
on united states pork	12
in the pig lung	12
approved the final manuscript	12
interaction between prrsv and	12
early stage of infection	11
the fact that the	11
immune response to the	11
nonneutralizing epitopes in the	11
monocytes macrophages on their	11
as a positive control	11
and european porcine reproductive	11
as an internal control	11
during infection of pigs	11
that the expression of	11
domain of porcine reproductive	11
a crucial role in	11
c in a humidified	11
replaced with fresh dmem	11
the multifunctionality of an	11
adaptive immune response to	11
structural proteins of porcine	11
institutional animal care and	11
highly pathogenic strain of	11
diversity of porcine reproductive	11
deubiquitinating and interferon antagonism	11
have shown that prrsv	11
until the end of	11
set of nonstructural proteins	11
the porcine respiratory disease	11
virulent porcine reproductive and	11
of type porcine reproductive	11
read and approved the	11
the results indicated that	11
of the genome of	11
pathogenicity of two us	11
cells infected with prrsv	11
the interaction between prrsv	11
the presence of virus	11
a concentration of mg	11
cells were stained with	11
immunity against porcine reproductive	11
was shown to be	11
of monocytes macrophages on	11
cells transfected with the	11
had no effect on	11
vaccines against porcine reproductive	11
neutralizing and nonneutralizing epitopes	11
of an intriguing set	11
antigen was detected in	11
was carried out using	11
comparative pathogenicity of nine	11
inhibition of prrsv replication	11
these data indicate that	11
to those of the	11
exploring the multifunctionality of	11
has been demonstrated that	11
the stability of the	11
to a lesser extent	11
in sows and respiratory	11
compared with the control	11
of neutralizing and nonneutralizing	11
end of the study	11
of porcine epidemic diarrhea	11
of the humoral immune	11
the inflammatory response to	11
epitopes in the porcine	11
multifunctionality of an intriguing	11
the cells were fixed	11
that the presence of	11
in an attempt to	11
development of adaptive immunity	11
was performed using a	11
common mucosal immune system	11
responses of swine to	11
by the end of	11
of the infected cells	11
statistical analyses were performed	11
and cellular immune responses	11
was no significant difference	11
intriguing set of nonstructural	11
were inoculated with prrsv	11
phosphorylation and nuclear translocation	11
and nonneutralizing epitopes in	11
genes involved in the	11
the presence of a	11
cells were incubated for	11
science foundation of china	11
in addition to the	11
of prrsv in the	11
a porcine reproductive and	11
with prrsv for h	11
cells and pam cultures	11
to be involved in	11
with the exception of	11
porcine alveolar macrophages and	11
results are expressed as	11
the number of cells	11
in the expression of	11
supernatants were collected and	11
an intriguing set of	11
from three independent experiments	11
they have no competing	11
passages in tissue culture	11
or swine influenza virus	11
response of swine to	11
american and european porcine	11
the n protein of	11
of gp and m	11
of cd t cells	11
infected with prrsv strain	11
the prrsv gp protein	11
plays a key role	11
for their ability to	11
macrophages on their susceptibility	11
persistence of porcine reproductive	11
at room temperature for	11
challenge with mn c	11
and infected with prrsv	11
studies are needed to	11
at the p position	11
member of the arterivirus	11
and cd t cells	11
infected with nc b	11
the mrna levels of	11
and lymphoid tissues of	11
well plates were co	11
respiratory syndrome virus entry	11
identification of neutralizing and	11
with regard to jurisdictional	10
c for s and	10
prrsv infection of marc	10
syndrome virus in swine	10
were washed with pbs	10
the endoribonuclease activity of	10
these data suggest that	10
dub activity of nsp	10
in published maps and	10
genetic diversity of european	10
may play a role	10
there was a significant	10
molecular characterization of porcine	10
were observed in the	10
coronavirus infectious bronchitis virus	10
protein in porcine reproductive	10
syndrome virus isolates in	10
have been identified as	10
state of differentiation and	10
and nuclear translocation of	10
than that of the	10
of prrsv nsp and	10
infection by porcine reproductive	10
protective immune response in	10
virus infection in pigs	10
van reeth and nauwynck	10
effects of origin and	10
type i ifn production	10
culture supernatants were collected	10
the orf gene of	10
status and future direction	10
of immune responses to	10
respiratory syndrome virus inhibits	10
north american type prrsv	10
the middle of the	10
jurisdictional claims in published	10
of the innate immune	10
of viral rna synthesis	10
the reciprocal of the	10
springer nature remains neutral	10
than those in the	10
immune responses in the	10
inflammatory response to porcine	10
commercial porcine reproductive and	10
atf and xbp s	10
pigs infected with nc	10
depletion of cellular cholesterol	10
with the presence of	10
and the percentage of	10
novel structural protein in	10
response to porcine respiratory	10
claims in published maps	10
with porcine circovirus type	10
postweaning multisystemic wasting syndrome	10
performed to determine the	10
was used for the	10
nonstructural protein of porcine	10
the presence of m	10
present in all arteriviruses	10
analyses were performed using	10
did not have an	10
of classical swine fever	10
repertoire development in fetal	10
virus replication in the	10
the plp dub activity	10
to jurisdictional claims in	10
version of this article	10
origin and state of	10
and state of differentiation	10
virus and porcine circovirus	10
in fetal and neonatal	10
type and type prrsv	10
current status and future	10
in the endoplasmic reticulum	10
between porcine reproductive and	10
immune response against prrsv	10
effect of porcine reproductive	10
n protein of prrsv	10
cysteine protease domain of	10
culture medium was replaced	10
protease domain of porcine	10
american strains of porcine	10
in dmem supplemented with	10
can be used to	10
is known about the	10
t cells in the	10
derived from the er	10
the number of cd	10
genotype porcine reproductive and	10
as a consequence of	10
in pigs vaccinated with	10
of type i interferon	10
reproductive and respiratory virus	10
the immune response against	10
response to prrsv infection	10
to the end of	10
were analyzed using the	10
the d kv adj	10
and porcine respiratory coronavirus	10
infection with prrsv and	10
immunological responses of swine	10
the cells were harvested	10
the use of a	10
exacerbates the inflammatory response	10
published maps and institutional	10
and the role of	10
data indicate that the	10
structural protein in porcine	10
mock infected or infected	10
were randomly assigned to	10
prrsv is an enveloped	10
well plates were transfected	10
the presence of ribavirin	10
national natural science foundation	10
in order to investigate	10
development in fetal and	10
is consistent with the	10
nature remains neutral with	10
of pigs at different	10
replication of prrsv and	10
nine us porcine reproductive	10
porcine alveolar macrophages in	10
in the middle of	10
as well as in	10
results suggest that the	10
was performed to determine	10
remains neutral with regard	10
a virulent strain of	10
did not affect the	10
maps and institutional affiliations	10
restriction fragment length polymorphism	10
immune response to porcine	10
our data indicate that	10
have shown that the	10
neutral with regard to	10
the nucleus and the	10
cells in the thymus	10
of nine us porcine	10
the type i ifn	10
that the e protein	10
regard to jurisdictional claims	10
there is a need	10
pathogenicity of nine us	10
significant increase in the	10
european isolates of porcine	10
role of porcine reproductive	10
north american type porcine	10
compared to those in	10
deletion in the nsp	10
by the presence of	10
of porcine respiratory and	10
were performed in triplicate	10
role of neutralizing antibodies	10
of origin and state	10
when the cells were	9
of vesicular stomatitis virus	9
a clinical and virological	9
is important for virus	9
play important roles in	9
of dendritic cell populations	9
immune response of pigs	9
essential for virus replication	9
prrsv infection in marc	9
the united states in	9
in the clearance of	9
the nuclear localization signal	9
and proteolytic processing in	9
chinese highly pathogenic strain	9
of the pi k	9
and porcine epidemic diarrhea	9
infected at an moi	9
the course of infection	9
with prrsv at a	9
by porcine respiratory coronavirus	9
interferon activation and susceptibility	9
not be detected in	9
infected with prrsv for	9
remains to be determined	9
infections of feeder pigs	9
immunosuppression exacerbates the inflammatory	9
between innate and adaptive	9
induced immunosuppression exacerbates the	9
characterization of proteins encoded	9
infection porcine reproductive and	9
the lungs and lymphoid	9
it is important to	9
arterivirus replicase orf a	9
hek t cells were	9
the context of prrsv	9
highly virulent porcine reproductive	9
our results suggest that	9
a chinese highly pathogenic	9
was replaced with fresh	9
respiratory syndrome virus a	9
the remainder of the	9
the case of prrsv	9
and systemic immune responses	9
in the early stage	9
virus in the lungs	9
treatment of cells with	9
alternative orf present in	9
their susceptibility to porcine	9
adaptive immune responses to	9
encoded proteinases and proteolytic	9
have no competing interests	9
neutralizing antibodies in prrsv	9
of the n protein	9
at the site of	9
that the prrsv e	9
to the proteins of	9
dendritic cells infected with	9
structural biology of prrsv	9
vr or mn c	9
our results indicated that	9
virus followed by porcine	9
supernatants were collected at	9
challenges for porcine reproductive	9
virus encoded by an	9
proteinases and proteolytic processing	9
activation and susceptibility of	9
after challenge with mn	9
was carried out with	9
structural protein of porcine	9
respiratory coronavirus or swine	9
the ability of prrsv	9
the mutant t g	9
was used as an	9
the open reading frame	9
inhibitory effect on the	9
nucleus and the cytoplasm	9
response to prrsv is	9
compared to the vector	9
of feeder pigs with	9
encoded by an alternative	9
african swine fever virus	9
the chinese highly pathogenic	9
fetal and neonatal piglets	9
type i interferon signaling	9
pcv a and pcv	9
followed by porcine respiratory	9
the acute phase of	9
the formation and nuclear	9
have been reported to	9
the structural biology of	9
syndrome virus in a	9
antiviral activity of ribavirin	9
vaccine with a potent	9
type i interferon activation	9
the antiviral effect of	9
cell populations to porcine	9
comparison of the north	9
a and pcv b	9
studies with the porcine	9
susceptibility of dendritic cell	9
would like to thank	9
dendritic cell populations to	9
processing in the nidovirales	9
orf present in all	9
and susceptibility of dendritic	9
prrsv and pedv were	9
on the development of	9
cells were maintained at	9
respiratory tract of pigs	9
with a chinese highly	9
load in the lungs	9
sites of virus replication	9
an equal volume of	9
of antigen presenting cells	9
and in vitro interferon	9
levels of neutralizing antibodies	9
of the equine arteritis	9
infected with prrsv or	9
the expression of prrsv	9
attenuation of porcine reproductive	9
was performed as previously	9
a broad range of	9
committee on taxonomy of	9
antibody response to prrsv	9
by plasmacytoid dendritic cells	9
syndrome virus strain vr	9
can be used as	9
equine arteritis virus replicase	9
the expression of small	9
kindly provided by dr	9
an increase in the	9
feeder pigs with porcine	9
virus in swine sera	9
were obtained from the	9
infection in gnotobiotic pigs	9
respiratory syndrome virus identification	9
and the presence of	9
the e protein is	9
molecular epidemiology of prrsv	9
prrsv gp and m	9
innate immune response to	9
the culture supernatants were	9
performed as previously described	9
of prrsv have been	9
in the lung and	9
the second lps administration	9
to induce neutralizing antibodies	9
an alternative orf present	9
cells were fixed and	9
dual infections of feeder	9
with porcine respiratory coronavirus	9
expressed as the mean	9
coronavirus or swine influenza	9
on the structural proteins	9
in subgenomic mrna synthesis	9
on taxonomy of viruses	9
for h at rt	9
by intranasal delivery of	9
i interferon activation and	9
emergence of a virus	9
increase in the frequency	9
terminal region of the	9
replication in cell culture	9
then washed three times	9
respiratory syndrome virus followed	9
the immune system and	9
failure of unknown etiology	9
a final concentration of	9
for min at rt	9
was extracted from the	9
for the first time	9
the authors declare no	9
by western blot analysis	9
of hepatitis c virus	9
the common mucosal immune	9
reproductive failure of unknown	9
play a key role	9
their ability to induce	9
were cloned into the	9
can be divided into	9
was performed in a	9
of type i interferons	9
immunization of pigs with	9
a protective immune response	9
likely due to the	9
washed twice with pbs	9
for the generation of	9
in response to prrsv	9
could not be detected	9
test for the detection	9
infection in pigs is	9
nsp and nsp were	9
pathogenic strain of porcine	9
north american isolates of	9
in a co atmosphere	9
cell lysates were prepared	9
syndrome virus followed by	9
protective immune response against	9
the induction of ifn	9
and is important for	9
as the reciprocal of	9
nucleocapsid protein of porcine	9
isolates in a five	9
the absence of e	9
encoded by orf b	9
most economically important diseases	9
by an alternative orf	9
may be related to	9
international committee on taxonomy	9
a new group of	9
was approved by the	9
cells were then washed	9
in this study was	9
in any of the	9
the nature of the	9
the replication of both	9
the viral genome and	9
knowledge on the structural	9
viral load in the	9
cholesterol is required for	9
five times in pbs	9
the respiratory tract of	9
test was used to	9
porcine respiratory coronavirus or	9
activation of b cells	9
vaccine against porcine reproductive	9
populations to porcine arterivirus	9
has been observed in	9
proteolytic processing in the	9
the first line of	9
between prrsv and the	9
were found in the	9
differential type i interferon	9
nine open reading frames	9
h and then infected	9
were washed five times	9
were washed once with	9
it is difficult to	9
on the basis of	9
the nsp nsp junction	9
was due to the	9
has the potential to	9
compared to that of	9
was found in the	9
analysis was performed using	9
clinical and virological study	9
are shown in table	9
the arterivirus replicase orf	9
infectious cdna clones of	9
monoclonal antibodies to the	9
the method of reed	9
losses to the swine	9
losses in the swine	9
of the presence of	9
against homologous and heterologous	9
of north american type	9
the proteins of porcine	9
rna was isolated from	8
respiratory syndrome virus during	8
containing fetal bovine serum	8
the pcr products were	8
the inhibition of prrsv	8
the presence of maternal	8
and future vaccine development	8
cells were stimulated with	8
induction of t helper	8
assessment of the efficacy	8
a second lps administration	8
the prrsv genome is	8
to of lelystad virus	8
based on measurement of	8
at various time points	8
induce the expression of	8
orfs to of lelystad	8
protein possesses deubiquitinating and	8
these data show that	8
the online version of	8
macrophage recruitment in porcine	8
acid deletion in the	8
were performed using the	8
were collected by centrifugation	8
to of porcine reproductive	8
the arterivirus nsp protease	8
involves both glycoprotein as	8
immune responses to the	8
in our previous study	8
were negative for prrsv	8
live virus vaccine with	8
cells were pretreated with	8
but not in the	8
pigs infected with the	8
after three washes with	8
prrsv strain jxwn plp	8
respiratory syndrome virus reveals	8
equine arteritis virus is	8
protein to the nucleolus	8
tissues of pigs at	8
there were no significant	8
in serum and semen	8
syndrome virus through glycan	8
by dendritic cells infected	8
cell and macrophage recruitment	8
both glycoprotein as well	8
tissue culture infective dose	8
serum immune responses to	8
in pigs is independent	8
that overlaps the gp	8
nuclear translocation of isgf	8
evolution of prrsv in	8
results indicate that the	8
respiratory syndrome viruses differ	8
significant economic losses to	8
to prrsv infection in	8
qiaamp viral rna mini	8
agents associated with respiratory	8
identification and characterization of	8
cells in the lungs	8
to the lack of	8
for the assembly of	8
and interferon antagonism functions	8
in agreement with previous	8
glycoprotein as well as	8
the structure of the	8
should be noted that	8
american type porcine reproductive	8
american isolates of porcine	8
of a virus subpopulation	8
gene that overlaps the	8
immune responses against prrsv	8
in the thymus of	8
was significantly reduced in	8
increase in the number	8
performed according to the	8
a significant reduction in	8
of nap on prrsv	8
play a critical role	8
of commercial porcine reproductive	8
virus nucleocapsid protein to	8
of the order nidovirales	8
caused by porcine reproductive	8
by inhibition of the	8
region of the arterivirus	8
those in the vector	8
as well as glycoprotein	8
producing cells and virological	8
discovery of a small	8
could be used to	8
virological parameters of protection	8
important for virus production	8
a virus subpopulation during	8
belonging to the family	8
expression levels of the	8
online version of this	8
and macrophage recruitment in	8
regulatory cells by dendritic	8
orfs to of porcine	8
to the absence of	8
syndrome virus strains of	8
the north american type	8
with paraformaldehyde for min	8
and then incubated with	8
ability of prrsv to	8
of a modified live	8
are presented as the	8
and transport of proteins	8
antibodies in prrsv protective	8
the interaction between trim	8
in this study we	8
normalized to that of	8
different time intervals after	8
with a mixture of	8
was carried out in	8
intervals after inoculation with	8
cells as described previously	8
detected in the serum	8
pigs is independent of	8
glycan shielding involves both	8
of protection upon challenge	8
virulent strain of porcine	8
polyclonal activation of b	8
t cell responses to	8
was determined to be	8
of the number of	8
sequence and is important	8
of a porcine reproductive	8
used to detect the	8
as compared to the	8
modulates host innate immune	8
pigs at different time	8
mucosal and systemic immune	8
vaccines based on measurement	8
it should be noted	8
family arteriviridae in the	8
virus nonstructural protein possesses	8
cells infected with porcine	8
from the endoplasmic reticulum	8
severe reproductive failure in	8
virus infection or vaccination	8
for prrsv and pedv	8
of t helper regulatory	8
the global swine industry	8
during entry of the	8
through glycan shielding involves	8
is in agreement with	8
study was supported by	8
of mouse hepatitis virus	8
de lima et al	8
to the site of	8
at rpm for min	8
were harvested and the	8
targets in the lungs	8
and virological parameters of	8
by inhibiting irf activation	8
by antagonizing irf activation	8
each of the two	8
of a small arterivirus	8
monkey kidney cell line	8
the plates were incubated	8
protein is essential for	8
on measurement of serologic	8
efficacy of commercial porcine	8
the virus from the	8
for the production of	8
the entry of prrsv	8
over the course of	8
to western blot analysis	8
of plp dub activity	8
plates were incubated for	8
morbidity and mortality in	8
statistical analysis was performed	8
a pivotal role in	8
a live virus vaccine	8
small arterivirus gene that	8
time intervals after inoculation	8
measurement of serologic response	8
to better understand the	8
den born et al	8
t helper regulatory cells	8
were performed according to	8
infected cells were maintained	8
induce the formation of	8
evasion of porcine reproductive	8
in immortalized porcine alveolar	8
end of the first	8
sodium azide in pbs	8
in the lung homogenate	8
that the n protein	8
parameters of protection upon	8
the specificity of the	8
may be involved in	8
nonstructural protein possesses deubiquitinating	8
possesses deubiquitinating and interferon	8
not induce treg cells	8
in prrsv protective immunity	8
dmem supplemented with fbs	8
immortalized porcine alveolar macrophages	8
all authors read and	8
reproductive failure induced by	8
is responsible for the	8
interaction between trim and	8
van gucht et al	8
of prrsv infection on	8
immune evasion of porcine	8
the level of virus	8
of the prrs virus	8
new group of positive	8
the autophagosomes induced by	8
is independent of virus	8
end of the genome	8
demonstrated that prrsv nsp	8
used as a negative	8
the expression of cd	8
nested set of six	8
key role in the	8
syndrome virus identification of	8
potentiation of porcine reproductive	8
in pigs inoculated with	8
two genotypes of prrsv	8
the efficacy of commercial	8
a small arterivirus gene	8
prrsv and mycoplasma hyopneumoniae	8
respiratory syndrome virus at	8
the viral loads in	8
experimentally infected with porcine	8
are fully resistant to	8
on prrsv replication was	8
virus infection in gnotobiotic	8
group was injected with	8
the p and p	8
arteriviridae in the order	8
tl co hb kg	8
the relative levels of	8
samples were collected from	8
the results of this	8
nucleocapsid protein to the	8
of european porcine reproductive	8
derived dendritic cells and	8
cells and virological parameters	8
inoculation with porcine reproductive	8
overlaps the gp coding	8
virus by monoclonal antibodies	8
to the arteriviridae family	8
both mucosal and systemic	8
economic losses in the	8
significantly higher levels of	8
responses to the proteins	8
nuclei were stained with	8
protection against reproductive failure	8
the family arteriviridae in	8
after inoculation with porcine	8
with prrsv and pedv	8
k fitsrcrl and f	8
was supported by a	8
recruitment in porcine reproductive	8
in infected pigs and	8
van den born et	8
implications for current vaccine	8
pigs were randomly assigned	8
cellular targets in the	8
gp coding sequence and	8
virus isolates in the	8
virus through glycan shielding	8
and emergence of a	8
our understanding of the	8
replicase orf a protein	8
experiments were performed in	8
transport of proteins encoded	8
tonsil and lymph nodes	8
the gp coding sequence	8
the replication cycle of	8
independent of virus load	8
randomly assigned to one	8
of cellular targets in	8
at different time intervals	8
under the control of	8
fitsrcrl and f gymtfvhf	8
assigned to one of	8
was significantly higher than	8
arterivirus gene that overlaps	8
irf phosphorylation and nuclear	8
as a model for	8
helper regulatory cells by	8
were no significant differences	8
coding sequence and is	8
a chimeric virus with	8
cells by dendritic cells	8
and c kv adj	8
syndrome viruses differ in	8
at the indicated time	8
shielding involves both glycoprotein	8
transmission of porcine reproductive	8
rule out the possibility	7
delivery of a live	7
nuclear accumulation of isgf	7
the immune response in	7
respiratory syndrome virus contains	7
of prrsv infection in	7
play a crucial role	7
sufficient to increase susceptibility	7
replicase is mediated by	7
probably due to the	7
detection of viral rna	7
gp protein encoded by	7
extracted from lysates of	7
in pigs experimentally infected	7
pigs upon experimental infection	7
rna was reverse transcribed	7
susceptibility of piglets to	7
used to evaluate the	7
vaccine efficacy and future	7
response in porcine reproductive	7
viral rna in serum	7
protein is the most	7
following infection with prrsv	7
was used to evaluate	7
endoribonuclease activity of nsp	7
the ectodomain of gp	7
against a heterologous prrsv	7
is sufficient to increase	7
the early stages of	7
of lelystad virus and	7
replication and sg mrna	7
derived dendritic cells but	7
to increase susceptibility of	7
reproductive syndrome virus strain	7
genomic rna replication and	7
no significant differences in	7
blocked the dub activity	7
response by antagonizing irf	7
rna virus belonging to	7
it is likely that	7
hyopneumoniae potentiation of porcine	7
protease and is essential	7
identification of cellular targets	7
macrophages and dendritic cells	7
virus vaccine with a	7
shown to interact with	7
cd and cd t	7
the first week of	7
the supernatants were collected	7
serum and semen of	7
north american prrsv strains	7
absence of e protein	7
was used for all	7
porcine high fever syndrome	7
viral loads in the	7
dendritic cells and compromises	7
of genomic rna and	7
three washes with pbs	7
entry of porcine reproductive	7
were considered to be	7
with d kv adj	7
the absence of a	7
the concentration of the	7
syndrome virus contains a	7
for simultaneous detection and	7
fold lower than that	7
infection remains to be	7
essential for porcine reproductive	7
comparison of the structural	7
was used to calculate	7
cdna clone of a	7
not related to its	7
of lipid rafts in	7
the majority of the	7
and sg mrna transcription	7
virus is sufficient to	7
syndrome virus infection or	7
mg kg body weight	7
may contribute to the	7
respiratory syndrome virus into	7
identification of a novel	7
incubated for min at	7
are expressed as the	7
expressing gp and m	7
were mock infected or	7
effect of prrsv on	7
associated with respiratory diseases	7
coding sequences of the	7
and its role in	7
treatment and prevention of	7
structural protein coding regions	7
remains to be elucidated	7
syndrome virus by monoclonal	7
the severity of clinical	7
of an infectious clone	7
comparison of lelystad virus	7
entry of the porcine	7
irf activation in immortalized	7
by nsp serine protease	7
in the activation of	7
and ability to induce	7
t cells were transfected	7
in the writing of	7
these findings suggest that	7
virus in pigs during	7
virus in infected pigs	7
were collected at different	7
directed mutagenesis of the	7
epidemic diarrhea virus infection	7
the infected pigs at	7
was measured at nm	7
as the negative control	7
of the parental virus	7
the host immune system	7
the activation of the	7
and respiratory disease in	7
has not yet been	7
membrane was incubated with	7
not a togavirus but	7
the transcript abundance of	7
n protein between the	7
efficacy and future vaccine	7
inhibit type i interferon	7
togavirus but belongs to	7
of arterivirus replicase is	7
ii porcine reproductive and	7
van breedam et al	7
glycosylation of porcine reproductive	7
vero cells were treated	7
lungs and lymphoid tissues	7
a result of the	7
and respiratory distress in	7
been shown to inhibit	7
on the presence of	7
were significantly higher in	7
at c for min	7
were used as the	7
of infection under farm	7
treated with ng ml	7
swine influenza h n	7
and transferred to a	7
challenge by streptococcus suis	7
cd expression confers susceptibility	7
type i ifn response	7
infected cells at hpi	7
the infected cells at	7
during the critical window	7
and identification of cellular	7
infected cells were then	7
of prrsv genomic rna	7
serine protease and is	7
detection of antibody to	7
viral rna was extracted	7
the purpose of this	7
and mycoplasma hyopneumoniae co	7
virus by intranasal delivery	7
a secondary antibody staining	7
the relative abundance of	7
quantification and identification of	7
at h after the	7
to the fact that	7
the highly pathogenic prrsv	7
inhibited the replication of	7
to challenge by streptococcus	7
but belongs to the	7
the etiological agent of	7
prrsv infectious cdna clone	7
virus nucleocapsid protein modulates	7
the titers of prrsv	7
increase susceptibility of piglets	7
subpopulation during infection of	7
i interferon signaling by	7
virus mystery swine disease	7
at h post infection	7
the evolution of porcine	7
well cell culture plates	7
and cloned into the	7
heart and lymphoid system	7
subtype porcine reproductive and	7
between the nucleus and	7
mediated by nsp serine	7
is mediated by nsp	7
open reading frame b	7
crystal structure of the	7
the presence of viral	7
reciprocal of the highest	7
and lymphoid system of	7
cells infected with isolate	7
a togavirus but belongs	7
viruses differ in non	7
data were analyzed using	7
amplified by pcr and	7
syndrome virus in serum	7
of the first week	7
sows and respiratory distress	7
response in the lungs	7
sequence analysis of the	7
syndrome virus variant associated	7
to determine whether the	7
set of subgenomic mrnas	7
with porcine high fever	7
syndrome virus infection of	7
at all time points	7
infected with porcine respiratory	7
green monkey kidney cell	7
confers susceptibility to porcine	7
related to its virulence	7
after challenge with vr	7
were positive for prrsv	7
that prrsv strain jxwn	7
virus and swine infertility	7
of mystery swine disease	7
this study was supported	7
incubated for an additional	7
and swine influenza virus	7
in the production of	7
of an inactivated vaccine	7
previous studies demonstrated that	7
nb activation by prrsv	7
and swine infertility and	7
care and use of	7
semen of adult boars	7
a highly pathogenic prrsv	7
van nieuwstadt et al	7
for the purpose of	7
and gp of porcine	7
and the north american	7
virus subpopulation during infection	7
was detected with a	7
were purchased from sigma	7
individual pigs upon experimental	7
virus in serum and	7
cd join forces during	7
variation of porcine reproductive	7
syndrome virus productively infects	7
we focused on the	7
number of infected cells	7
no significant difference in	7
characterization of the humoral	7
syndrome virus gp on	7
virus gp on virus	7
china is not related	7
porcine high fever disease	7
it is clear that	7
but does not induce	7
expressed as the reciprocal	7
experimental porcine reproductive and	7
in the heart and	7
inhibits the replication of	7
the time of infection	7
classical north american type	7
are shown in fig	7
virus variant associated with	7
were performed for each	7
were shown to be	7
respiratory syndrome virus type	7
in the nsp of	7
the hepatitis c virus	7
emerging in china is	7
development of the interferon	7
was used in this	7
early stages of infection	7
recovery of virus replication	7
we would like to	7
of the severe acute	7
respiratory syndrome virus isolate	7
of piglets to challenge	7
was significantly higher in	7
associated with porcine high	7
did not affect viral	7
in china is not	7
reverse genetics system for	7
is not a togavirus	7
recombination between north american	7
nsp of highly pathogenic	7
current vaccine efficacy and	7
blocked with bovine serum	7
ability to induce neutralizing	7
detection of antibodies to	7
the heart and lymphoid	7
by in situ hybridization	7
the s specificity pocket	7
in individual pigs upon	7
forces during entry of	7
note springer nature remains	7
simultaneous detection and differentiation	7
lelystad virus and swine	7
were determined using the	7
the data presented in	7
chinese highly pathogenic porcine	7
of clinical signs and	7
phenotype and genotype diversity	7
clone of a highly	7
of the sdd motif	7
the duration of the	7
in the sdd motif	7
quasispecies and emergence of	7
significant difference between the	7
of the structural protein	7
the level of atpase	7
respiratory syndrome virus molecular	7
virus and establishes sterilizing	7
between north american strains	7
is also possible that	7
immune response by antagonizing	7
cells were then incubated	7
of a canadian porcine	7
antigenic comparison of lelystad	7
at c for h	7
located in the middle	7
on ice for min	7
porcine circovirus type and	7
processing of the open	7
treated with dnase i	7
the north american genotype	7
the membrane was incubated	7
host response to prrsv	7
is involved in the	7
was used to determine	7
properties of tulathromycin in	7
the mechanism of prrsv	7
mediated innate immunity and	7
of prrsv in a	7
washed once with pbs	7
failure induced by a	7
syndrome virus and establishes	7
the intensity of the	7
the major structural proteins	7
it was demonstrated that	7
syndrome virus in china	7
with type porcine circovirus	7
of pigs with a	7
level of atpase activity	7
be attributed to the	7
the development of an	7
intranasal delivery of a	7
at a ratio of	7
aliquoted and stored at	7
respiratory syndrome virus variant	7
virus quantification and identification	7
virus is not a	7
of transmissible gastroenteritis virus	7
against reproductive failure induced	7
and cd join forces	7
a canadian porcine reproductive	7
at a density of	7
closely related to the	7
with respiratory diseases in	7
the absence of any	7
syndrome virus in semen	7
into porcine alveolar macrophages	7
molecular evolution of prrsv	7
evaluation of immune responses	7
formation and nuclear translocation	7
the international committee on	7
the existence of two	7
heterogeneity of porcine reproductive	7
collected by centrifugation at	7
cells were subjected to	7
the virus inoculum was	7
type i interferon induction	7
viral titers were measured	7
viral rna mini kit	7
gp on virus infectivity	7
activation in immortalized porcine	7
dna and rna viruses	7
standard error of the	7
expression of prrsv nsp	7
encoded part of arterivirus	7
cells were incubated at	7
lower than those in	7
the national natural science	7
virus and related viruses	7
the writing of the	7
to induce protection against	7
of a live virus	7
under the same conditions	7
dendritic cells but does	7
part of arterivirus replicase	7
transcription polymerase chain reaction	7
piglets to challenge by	7
for nsp to inhibit	7
due to the absence	7
virus productively infects monocyte	7
of plasma membrane cholesterol	7
the production of ifn	7
statistical significance was determined	7
by a virulent strain	7
in pigs of all	7
of the virus from	7
and establishes sterilizing immunity	7
does not induce treg	7
as a template for	7
the care and use	7
and evolution of porcine	7
prrsv n protein was	7
then inoculated with prrsv	7
the synthesis of prrsv	7
length sequence of a	7
the control pigs at	7
persistence in individual pigs	7
mycoplasma hyopneumoniae potentiation of	7
that the pi k	7
the expression of trim	7
with the expression of	7
for the study of	7
of the arterivirus replication	7
and compromises their antigen	7
could be due to	7
of pigs with vr	7
sialoadhesin and cd join	7
were monitored daily for	7
in the lymph nodes	7
in pigs during early	7
pigs were negative for	7
the inhibition of ifn	7
acid sequence of nsp	7
sequence of a canadian	7
the gp and gp	7
antigen in the heart	7
the detection of antibody	7
prevention and control of	7
respiratory syndrome virus productively	7
with prrsv or pedv	7
be found in the	7
and semen of adult	7
for current vaccine efficacy	7
cells and compromises their	7
in agreement with the	7
prrsv infection and the	7
dendritic cells to porcine	7
ability of the virus	7
presence of maternal antibodies	7
standard curve was generated	7
in porcine nidovirus infection	7
no conflict of interest	7
of the prrsv n	7
plates were transfected with	7
by streptococcus suis type	7
were measured using a	7
the role of porcine	7
nonstructural and structural proteins	7
expression confers susceptibility to	7
of the gp ectodomain	7
of genes involved in	7
pigs did not show	7
or a combination of	7
of the chimeric vlps	7
an inhibitory effect on	7
canadian porcine reproductive and	7
nsp serine protease and	7
were introduced into the	7
states porcine reproductive and	7
inhibiting irf activation in	7
were inoculated with acaprrs	7
to the major structural	7
used for detection of	7
was also observed in	7
highly pathogenic prrsv strain	7
approximately kb in length	7
for the swine industry	7
cd cd t cells	7
during early stage of	7
to investigate whether the	7
modulating properties of tulathromycin	7
no effect on the	7
gradual development of the	7
join forces during entry	7
prrsv infection in the	7
of green fluorescent protein	7
emergence of highly pathogenic	7
description of persistence in	7
pigs during early stage	7
stage of infection under	7
evolving the largest rna	7
the number of infected	7
syndrome virus is sufficient	7
nucleic acid sequence of	7
after vaccination with a	7
amino acid sequences of	7
prrsv antigen was detected	7
sasa quelpaertensis nakai extract	7
after removal of the	7
have been used as	7
were stained with dapi	7
how to make sense	7
the clearance of prrsv	7
response against prrsv is	7
cells but does not	7
the active site loop	7
cells of the monocyte	7
of the open reading	7
and reproductive failure in	7
of interferon regulatory factor	7
washed five times in	7
it is also possible	7
syndrome virus by intranasal	7
the indicated time points	7
in their ability to	7
the swine industry in	7
following infection with porcine	7
the host innate immune	7
variant associated with porcine	7
streptococcus suis type ii	7
replication of prrsv was	7
of the host response	7
is not related to	7
induced by a virulent	7
used as a control	7
of persistence in individual	7
arterivirus replicase is mediated	7
infection under farm conditions	7
elevating virus and related	7
it is known that	7
and is essential for	7
sequences of the vr	7
the nsp of highly	7
variation in porcine reproductive	6
prevention of porcine reproductive	6
cells supporting virus replication	6
essential for nsp to	6
viral respiratory disease in	6
an essential role in	6
with highly pathogenic porcine	6
adaptive immunity against porcine	6
is required for the	6
in the modulation of	6
neutralizing antibody response to	6
new insights into the	6
immune response to infection	6
the data indicated that	6
cellular rna was extracted	6
the pigs infected with	6
between trim and rig	6
homologous and heterologous challenges	6
of balb c mice	6
endoribonuclease nendou exerts pleiotropic	6
of the unfolded protein	6
detected in the lungs	6
interaction between nsp and	6
infected pigs compared with	6
were washed with cold	6
infected with type porcine	6
the viral genome is	6
due to porcine reproductive	6
bovine viral diarrhea virus	6
immune dysregulation is caused	6
an inactivated vaccine against	6
of the nidovirus replicative	6
vaccine investigated by parallel	6
on porcine alveolar macrophages	6
due to lack of	6
swine with a chinese	6
shuffling of the gp	6
resulting in immune dysregulation	6
contains a cluster of	6
which was associated with	6
virus comparative pathogenicity of	6
ribosomes to synthesize an	6
respiratory syndrome virus immune	6
complete nucleotide sequence of	6
production by interfering with	6
as early as days	6
the mechanisms by which	6
lymphoid system of three	6
the nsp main proteinase	6
is caused by porcine	6
on prrsv replication in	6
to be associated with	6
protected from porcine reproductive	6
at c in a	6
of new genetic subtypes	6
for detection of porcine	6
virus strains of exceptional	6
was to characterize the	6
their ability to cause	6
nap on prrsv replication	6
specificity of the immune	6
we also found that	6
transducer and activator of	6
for foreign gene expression	6
coding regions for nonstructural	6
nucleocapsid protein modulates interferon	6
whole cell lysate of	6
the critical window of	6
ability against a heterologous	6
to control prrsv infection	6
to the parental virus	6
levels of pedv n	6
has been used to	6
with an improved cross	6
in eastern europe support	6
macrophages porcine reproductive and	6
a novel structural protein	6
were divided into three	6
products were purified using	6
respiratory diseases in pigs	6
a significant role in	6
the arterivirus life cycle	6
at dpi and the	6
infection in porcine lung	6
activity did not affect	6
at the concentration of	6
strains of the prrs	6
gnotobiotic pigs porcine reproductive	6
were added to the	6
was propagated in marc	6
maintained in fresh medium	6
was extracted from lysates	6
crystal structure of prrsv	6
of north american and	6
middle east respiratory syndrome	6
envelope protein requirements for	6
for h and were	6
of prrsv or pedv	6
hepatitis c virus ns	6
syndrome virus in infected	6
of pigs following vaccination	6
to investigate the effect	6
the predicted helicase of	6
virus strains of the	6
was calculated using the	6
of two genotypes of	6
with the indicated antibodies	6
solutions for treatment and	6
between the presence of	6
were fixed with paraformaldehyde	6
were treated with m	6
kda structural protein of	6
least open reading frames	6
combination of p and	6
into the porcine macrophage	6
and prevention of porcine	6
localization signal of the	6
mucosal and systemic sites	6
be detected in the	6
and found that the	6
pigs coinfected with a	6
novel structural protein of	6
performed in accordance with	6
reduced ability to induce	6
from an infectious clone	6
respiratory syndrome virus using	6
existence of two genotypes	6
at a dilution of	6
method of estimating fifty	6
of positive strand rna	6
of african swine fever	6
has been found to	6
to test the stability	6
prrsv infection in porcine	6
observed in the infected	6
be responsible for the	6
to be able to	6
after infection with prrsv	6
were approved by the	6
hek cells were transfected	6
support the definition of	6
lower in iln than	6
and evasion strategies for	6
a high mutation rate	6
immune responses of pigs	6
infected pigs at dpi	6
in their susceptibility to	6
these results demonstrate that	6
the midwestern united states	6
american strains of prrsv	6
european strains of porcine	6
respiratory syndrome virus emerged	6
hypotheses in porcine reproductive	6
characterization of a new	6
ifa as described above	6
the development of new	6
we demonstrated that the	6
united states swine with	6
the plates were washed	6
no inhibitory effect on	6
serum samples were collected	6
with different isolates of	6
there was no evidence	6
lymphoid hyperplasia resulting in	6
nsp was essential for	6
was measured using a	6
of the highly pathogenic	6
collected at different time	6
growth of porcine reproductive	6
and differentiation of hp	6
were used in this	6
and replication of porcine	6
chimeric construction with vaccine	6
link between innate and	6
of prrsv nsp in	6
of the gp genes	6
tomography of porcine reproductive	6
syndrome virus in porcine	6
time pcr was performed	6
as a source of	6
epitope of porcine respiratory	6
protective immunity to porcine	6
responses and evasion strategies	6
paraformaldehyde for min at	6
major histocompatibility complex class	6
vitro prrsv and mycoplasma	6
of prrsv helicase was	6
exceptional diversity in eastern	6
perspective from the united	6
were challenged with prrsv	6
united states porcine reproductive	6
the titer of prrsv	6
fixed in neutral buffered	6
effects on the arterivirus	6
from lysates of the	6
with various strains of	6
these data suggested that	6
been found to be	6
arteritis virus is not	6
eastern europe support the	6
porcine circovirus type in	6
that the ability of	6
study was to characterize	6
was detected in all	6
pigs were monitored daily	6
response and persistence of	6
genome of lelystad virus	6
are representative of the	6
to inhibit prrsv replication	6
epithelial cells of the	6
respiratory syndrome virus non	6
r d systems inc	6
simple method of estimating	6
control of prrsv infection	6
in immune dysregulation is	6
total cellular rna was	6
complete genome analysis of	6
representative of the mean	6
in type i ifn	6
stat stat nuclear translocation	6
of the two major	6
protein of equine arteritis	6
gilts and multiparous sows	6
a major cause of	6
of virus replication with	6
the gp genes of	6
in cells transfected with	6
and the development of	6
associated with prrsv infection	6
combination of both organisms	6
the transcription factor complex	6
the use of peptide	6
like porcine reproductive and	6
under a fluorescence microscope	6
were inserted into the	6
pbs for min at	6
compared to the control	6
were obtained from a	6
may play an important	6
were removed from the	6
isolates in the midwestern	6
syndrome virus gp protein	6
against a heterologous challenge	6
was used in the	6
a heterologous prrsv strain	6
modulation of host cell	6
critical role in the	6
generation of an infectious	6
was associated with a	6
protein has been shown	6
be used as an	6
pleiotropic effects on the	6
it was reported that	6
as indicated by the	6
at a dose of	6
porcine epidemic diarrhoea virus	6
pams were transfected with	6
infectious virions of porcine	6
to prrsv in utero	6
more severe clinical signs	6
of human immunodeficiency virus	6
of phenotype and genotype	6
fixed with paraformaldehyde for	6
with an antigenically distinct	6
as well as a	6
the detection of antibodies	6
belonging to the arteriviridae	6
protection against prrsv infection	6
room temperature for min	6
to the use of	6
type isolate of porcine	6
proinflammatory cytokines and viral	6
and h n influenza	6
there was no effect	6
beta modulates host innate	6
spread of the virus	6
i interferon production by	6
regions for nonstructural and	6
mrna levels of pedv	6
samples were positive for	6
and respiratory syndrome to	6
in the lung parenchyma	6
to protect gilts against	6
confers protection against reproductive	6
it is reasonable to	6
have been observed in	6
the complete nucleotide sequence	6
evasion strategies for porcine	6
antibodies directed against the	6
for the care and	6
in the immune system	6
the efficacy of a	6
in the viral envelope	6
the type i interferon	6
expression of both tnf	6
frameshifting by mammalian ribosomes	6
cells were washed once	6
page and transferred to	6
not included in the	6
geographical dissection of north	6
in most of the	6
genetic variation in porcine	6
for the analysis of	6
the c kv adj	6
be explained by the	6
of coding regions for	6
the viral copy numbers	6
essential genes a and	6
when compared to those	6
to be the major	6
of six or seven	6
and geographical dissection of	6
and control of the	6
characterization of antigenic regions	6
a continuous cell line	6
south dakota state university	6
primer pairs sf sr	6
antigenic regions in the	6
the cells were treated	6
pigs vaccinated with prrs	6
the site of infection	6
a simple method of	6
proteins of the virion	6
a perspective from the	6
the possibility that the	6
a vital role in	6
used as the negative	6
most of the virus	6
tropism in cell culture	6
syndrome virus entry into	6
have an effect on	6
in the innate immune	6
used to determine the	6
the virus life cycle	6
of cd and cd	6
failure of an inactivated	6
as shown by the	6
immune correlates of protection	6
clearance of the virus	6
binding motif of sla	6
in the first experiment	6
three times with cold	6
for min on ice	6
genetic diversity of the	6
the number of prrsv	6
production by inhibiting irf	6
lysates of the infected	6
domains i and ii	6
effect of tomatidine on	6
influenza a virus in	6
and evolution of the	6
protein requirements for the	6
to the coronaviruslike superfamily	6
inhibitory effect of nap	6
and viral respiratory disease	6
pigs naturally infected with	6
set of six or	6
various strains of porcine	6
persistence of the porcine	6
with the n protein	6
may be important for	6
activity of nsp was	6
virus and mycoplasma hyopneumoniae	6
is the causative agent	6
in a continuous cell	6
syndrome virus strain mn	6
cd t cells in	6
in balb c mice	6
expression of type i	6
response of pigs following	6
were separated by sds	6
and hypotheses in porcine	6
onset of humoral immunity	6
were used for the	6
porcine circovirus type infection	6
are derived from the	6
for the existence of	6
acute phase of infection	6
is the most abundant	6
the assembly of infectious	6
and then treated with	6
of the putative m	6
were incubated for an	6
following vaccination with various	6
prrsv infection has been	6
produces a chimeric virus	6
respiratory syndrome virus immunobiology	6
nested set of subgenomic	6
then infected with prrsv	6
the us prrsv vr	6
required for prrsv replication	6
carried out with the	6
dysregulation is caused by	6
as the secondary antibody	6
protein coding sequences of	6
from pigs infected with	6
results suggested that the	6
role in the clearance	6
investigated by parallel mutations	6
on the arterivirus life	6
nendou exerts pleiotropic effects	6
in the level of	6
inactivated vaccine against porcine	6
of humoral immune response	6
the nidovirus replicative endoribonuclease	6
respiratory syndrome to protect	6
synergism between porcine reproductive	6
jxwn at an moi	6
the activation of autophagy	6
virus encoded by orf	6
the clearance of the	6
of the porcine respiratory	6
electron tomography of porcine	6
of host cell responses	6
european subtype porcine reproductive	6
regions in the porcine	6
a combination of both	6
innate immune response and	6
the antiviral function of	6
europe and north america	6
at h post transfection	6
separation of coding regions	6
was significantly lower in	6
de vries et al	6
by nsp and nsp	6
internalized viruses were titrated	6
was significantly increased in	6
includes equine arteritis virus	6
in a previous study	6
this article can be	6
were used to detect	6
the protection conferred by	6
followed by incubation with	6
expression and replication of	6
of infectious cdna clones	6
replicative endoribonuclease nendou exerts	6
cd in the lipid	6
nucleocapsid protein attenuate virus	6
nsp to inhibit ifn	6
by mammalian ribosomes to	6
virus vaccine investigated by	6
the later stages of	6
rna virus with a	6
interfering with the rig	6
transferred to a nitrocellulose	6
did not result in	6
studies have indicated that	6
positive strand rna virus	6
heterologous challenge with prrsv	6
their susceptibility to prrsv	6
expanding diversity of porcine	6
these results are consistent	6
is required for porcine	6
functional properties of the	6
the african green monkey	6
were infected at an	6
the rjxwn challenge and	6
the absence of prrsv	6
respiratory syndrome viruses in	6
for replication in cell	6
results of this study	6
origin and evolution of	6
method of reed and	6
of a novel structural	6
cells were harvested at	6
at g for min	6
to evaluate the efficacy	6
efficacy of porcine reproductive	6
after the second lps	6
of infectious virions of	6
supported by the national	6
vaccinated pigs had significantly	6
field isolates of prrsv	6
the proteolytic activity of	6
current state of play	6
in the control group	6
virus with an improved	6
proteins of lactate dehydrogenase	6
the lungs of prrsv	6
a standard curve was	6
lelystad virus strains of	6
foot and mouth disease	6
primary pams treated with	6
significant differences between the	6
will be required to	6
diagnosis of porcine reproductive	6
prrsv replication in vitro	6
europe support the definition	6
virions of porcine reproductive	6
then incubated for h	6
doubts and hypotheses in	6
with different concentrations of	6
syndrome virus vaccine investigated	6
partial protection against prrsv	6
the structural protein coding	6
been shown that the	6
was no effect of	6
membrane cholesterol is required	6
the data suggest that	6
experimental infection of united	6
reproductive failure in pregnant	6
of cholesterol in the	6
are protected from porcine	6
construction with vaccine sequence	6
monitored for clinical signs	6
in the sera of	6
the nucleocapsid protein n	6
was essential for nsp	6
cytokines and viral respiratory	6
specific cd t cells	6