quadgram

This is a table of type quadgram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

quadgram	frequency
severe acute respiratory syndrome	212
porcine epidemic diarrhea virus	144
acute respiratory syndrome coronavirus	113
in the presence of	91
in vero e cells	88
cells were infected with	80
of severe acute respiratory	66
of porcine epidemic diarrhea	58
infected vero e cells	47
as shown in fig	43
for the treatment of	42
vero cells were infected	40
in vitro and in	38
in the present study	38
at an moi of	38
in the absence of	38
the antiviral activity of	37
cells were treated with	37
vitro and in vivo	36
african green monkey kidney	35
on vero e cells	35
coronavirus infectious bronchitis virus	35
of the s protein	34
the severe acute respiratory	32
washed three times with	32
herpes simplex virus type	31
of vero e cells	30
the cells were fixed	30
the intracellular extracts of	29
cells were fixed with	29
cells were incubated with	29
gds and gds strains	28
with severe acute respiratory	28
with fetal bovine serum	27
a and icpc a	27
icpc a and icpc	27
cells were washed twice	27
rna was extracted from	27
middle east respiratory syndrome	27
vero cells infected with	26
in the united states	25
on the other hand	25
were washed twice with	25
the cells were washed	25
was used as a	25
as well as the	24
the vero cell line	24
has been shown to	24
the gds and gds	23
for the production of	23
at c for h	23
in cells infected with	23
after h of incubation	23
porcine reproductive and respiratory	22
as a negative control	22
in the case of	22
determined by plaque assay	22
supplemented with fetal bovine	22
epidemic diarrhea virus in	22
reproductive and respiratory syndrome	22
the vero cells and	21
was added to the	21
effect on vero e	21
used in this study	21
the recombinant ibvs and	21
of the viral genome	20
and respiratory syndrome virus	20
s s cleavage site	20
washed twice with pbs	20
at a moi of	20
total rna was extracted	19
an important role in	19
incubated for h at	19
pcr and western blotting	19
vero e cells were	19
cells were inoculated with	19
east respiratory syndrome coronavirus	19
presence or absence of	19
at the plasma membrane	19
and vero cells were	19
at a multiplicity of	19
a multiplicity of infection	19
intracellular extracts of ln	18
in vero cells and	18
added to each well	18
were washed three times	18
cells were washed with	18
cells were seeded in	18
cells were fixed and	18
the presence or absence	18
h at room temperature	18
of blfp crude extract	18
recombinant ibvs and beaudette	18
three times with pbs	18
vero e cells exposed	17
for the detection of	17
the coronavirus infectious bronchitis	17
avian infectious bronchitis virus	17
vero cells were seeded	17
isolation and characterization of	17
in the vero cells	17
cell fusion activity of	17
e cells exposed to	17
of a novel coronavirus	17
at different time points	17
in the presence or	17
were washed with pbs	17
the s s cleavage	16
propagated in vero cells	16
the cells were incubated	16
vero cells and ipec	16
pcd and vero cells	16
for h at room	16
infectivity of icpc a	16
for the development of	16
the pedv s protein	16
the vero e cells	16
cells were transfected with	16
cells infected with the	16
pedv in vero cells	16
african swine fever virus	16
was added to each	15
plates were infected with	15
the s protein of	15
these results indicated that	15
min at room temperature	15
than that of the	15
egfr pi k akt	15
at a concentration of	15
the inoculum was removed	15
the porcine epidemic diarrhea	15
these results suggest that	15
vero e and ma	15
in the s protein	15
cells in the presence	15
was used as the	15
vero and a cells	15
it is important to	15
the spike protein of	15
the sam binding site	15
the expression levels of	15
in pbs for min	15
vero e cells in	14
at room temperature for	14
and the cells were	14
infected with wild type	14
vero e cells and	14
used as a negative	14
a member of the	14
the antiviral effect of	14
was performed as described	14
with different concentrations of	14
was removed and the	14
were fixed and stained	14
incubated at c for	14
in the treatment of	14
acute respiratory syndrome in	14
vero cells treated with	14
the results showed that	14
treated with or without	14
in cells transfected with	14
there was no significant	13
in the sam binding	13
one of the most	13
the infectivity of icpc	13
in the vero cell	13
cytopathic effect on vero	13
of herpes simplex virus	13
and characterization of a	13
the cells were collected	13
for min at room	13
patients with severe acute	13
is one of the	13
the invasion rates of	13
at hpi and hpi	13
at the same time	13
of the mutant virus	13
vero cells were treated	13
were incubated for h	13
c in a co	13
viral rna was extracted	13
were infected with pedv	13
inoculum was removed and	13
infection of vero cells	13
green monkey kidney cells	12
infection in vero cells	12
with pedv strains at	12
cells were grown in	12
with the recombinant ibvs	12
porcine epidemic diarrhoea virus	12
cells were incubated for	12
is a member of	12
in vero cells infected	12
in vero e and	12
the infectivity of the	12
of the gds and	12
vero cells were inoculated	12
were infected with hsv	12
at rt for min	12
had no effect on	12
regulation of cell death	12
in the number of	12
associated with severe acute	12
been shown to be	12
characterization of a novel	12
monolayers of vero cells	12
well plates were infected	12
with wild type and	12
the in vitro prophylactic	12
the expression of the	12
the authors declare that	12
with the intracellular extracts	12
these results suggested that	12
were determined by plaque	12
cells transfected with the	11
avian coronavirus infectious bronchitis	11
the presence of increasing	11
this work was supported	11
the vero e and	11
of the coronavirus infectious	11
of the rescued virus	11
d d d d	11
a fixed concentration of	11
cells were cultured in	11
for min at rt	11
a novel coronavirus associated	11
infected vero cells were	11
compared to those of	11
on the surface of	11
of infectious bronchitis virus	11
as compared to the	11
fixed concentration of sars	11
cells were maintained in	11
the treatment of sars	11
the development of a	11
the production of viral	11
coronavirus associated with severe	11
cells were pretreated with	11
to those of the	11
to a fixed concentration	11
fusion activity of s	11
was observed in the	11
treated with different concentrations	11
of vero cells with	11
compared to the parental	11
was determined using the	11
porcine epidemic diarrhea in	11
monkey kidney cell line	11
cells were collected at	11
against pedv in vero	11
the invasion efficiency of	11
novel coronavirus associated with	11
infected with pedv strains	11
were infected with the	11
length cdna clone of	11
were detected in the	11
cells infected with wild	11
pi k akt pathway	11
and incubated for h	11
the vero and ipec	11
fusion activity of the	11
propagation in vero cells	11
in vero cells in	11
at a density of	11
our results suggest that	11
as a result of	11
were collected at hpi	11
cells exposed to a	11
exposed to a fixed	11
the vero cells were	11
lower than that of	11
were fixed with paraformaldehyde	11
the presence of ribavirin	10
presence of increasing concentrations	10
carrying mutations in the	10
the vero cell lineage	10
a concentration ranging from	10
centers for disease control	10
the end of the	10
at a concentration ranging	10
the plasma membrane and	10
rates of the gds	10
pseudovirions with uncleaved s	10
it is possible that	10
washed with pbs and	10
results showed that the	10
m f and c	10
used at a concentration	10
american type culture collection	10
of the recombinant ibvs	10
against herpes simplex virus	10
mutations in the sam	10
and then incubated with	10
an equal volume of	10
of peptide h on	10
the vero e cell	10
in vitro prophylactic effects	10
the study of the	10
of increasing concentrations of	10
the early stages of	10
were observed in the	10
replication in vero e	10
was carried out by	10
was extracted from the	10
of the murine coronavirus	10
g a and rmv	10
of the spike protein	10
g for min at	10
the presence of trypsin	10
fixed and stained with	10
in patients with severe	10
with ck ch ldl	10
have been shown to	10
of prrsv and pedv	10
supernatants were collected at	10
and in the ipec	10
propagation of the virus	10
and in the vero	10
at h post infection	10
were inoculated with kpedv	10
mechanism of action of	10
cells were washed three	10
work was supported by	10
vero cells were cultured	10
at the end of	10
these data suggest that	10
containing the empty vector	10
identification of a novel	10
were inoculated with the	10
in the footpad with	10
bile and bile acids	10
of the porcine epidemic	10
culture supernatants were collected	10
proteolytic cleavage of the	10
room temperature for h	10
vero cells were grown	10
of the in vitro	10
in the culture medium	10
at h after the	10
the inhibitory effect of	10
infectious cdna clone of	10
studies have shown that	10
be due to the	9
in vitro antiviral activity	9
and then infected with	9
apoptosis in vero e	9
p mapk signaling pathway	9
the cells were then	9
the overexpression of the	9
at the time of	9
was amplified by rt	9
cells containing the empty	9
after washing three times	9
of vero cells were	9
of sabin poliovirus type	9
production of viral vaccines	9
of the viral rna	9
of the virus of	9
was observed in cells	9
epidemic diarrhea in cell	9
strains at h after	9
when compared to the	9
from patients recovered from	9
to that of the	9
rna was isolated from	9
for disease control and	9
it was found that	9
the p mapk signaling	9
by plaque assay and	9
of the helicase protein	9
and gds strains were	9
the regulation of cell	9
fusion of the viral	9
of pedv icpc a	9
ns a and ns	9
antiviral activity of ribavirin	9
of mouse hepatitis virus	9
invasion efficiency of pedv	9
error bars represent standard	9
e protein mab g	9
without blfp crude extract	9
the results of the	9
al kassaa et al	9
cells were overlaid with	9
sialic acid binding activity	9
pedv icpc a and	9
epidemic diarrhea virus strains	9
of the sars coronavirus	9
the virus of porcine	9
may be due to	9
of icpc a and	9
crude extract against pedv	9
by the addition of	9
was found to be	9
of cell death during	9
suggesting that myricetin may	9
activation of p mapk	9
the growth properties of	9
as described above and	9
diarrhea in cell culture	9
blfp crude extract against	9
the ns a protein	9
the focus forming assay	9
and vero e cells	9
well tissue culture plates	9
vero e cell line	9
virus of porcine epidemic	9
we determined that the	9
the results indicated that	9
added to the cells	9
the cells were infected	9
infection in vero e	9
incubated at room temperature	9
flavivirus e protein mab	9
the infected cells were	9
mers pseudovirions with uncleaved	9
vero e cells are	9
at the indicated times	9
associated with diarrhea in	8
disease control and prevention	8
green monkey kidney cell	8
by the severe acute	8
infected with an moi	8
vero cells and and	8
and r l mutant	8
of african green monkey	8
cells and and in	8
epidemic diarrhea virus infection	8
was significantly lower than	8
fixed with paraformaldehyde in	8
the total number of	8
it was shown that	8
in cell cultures and	8
blood samples were collected	8
type i and type	8
washing three times with	8
feline infectious peritonitis virus	8
were observed in cells	8
of the viral envelope	8
the two cell lines	8
results showed that pedv	8
an african green monkey	8
absence of growth factors	8
syndrome coronavirus spike protein	8
myricetin may interact with	8
s protein of pedv	8
in these cell lines	8
and hpi for qrt	8
a novel coronavirus from	8
cells were stained with	8
for h at c	8
cells were incubated at	8
the african green monkey	8
results indicated that the	8
and all of them	8
aminopeptidase n is a	8
e and huh cells	8
the absence of growth	8
passages in cell culture	8
was no significant difference	8
a and ns b	8
line of african green	8
similar to that of	8
induction of apoptosis by	8
is thought to be	8
pedv strains at h	8
medium was removed and	8
collected at hpi and	8
the induction of apoptosis	8
of three independent experiments	8
for the inhibition of	8
were considered statistically significant	8
abortus and chlamydia pecorum	8
syndrome in hong kong	8
institutional animal care and	8
experimental infection of pigs	8
were infected with an	8
vero cells grown in	8
protein of severe acute	8
was determined relative to	8
in each group were	8
in vero and ipec	8
acute respiratory distress syndrome	8
care and use committee	8
respiratory syndrome in hong	8
were infected with a	8
on the infection of	8
respiratory syndrome coronavirus spike	8
the viral titers in	8
the world health organization	8
r l mutant viruses	8
authors declare that they	8
the plates were incubated	8
and and in the	8
intracellular extracts of ypk	8
animal care and use	8
culture supernatants were harvested	8
a functional receptor for	8
viral rna synthesis and	8
the medium was removed	8
at various time points	8
glycoprotein of murine coronavirus	8
and infected with pedv	8
was determined by plaque	8
the international committee on	8
variants of porcine epidemic	8
fold serial dilutions of	8
were determined using the	8
e cells in the	8
with an moi of	8
chlamydia abortus and chlamydia	8
twice with pbs and	8
phosphorylation of p mapk	8
acquisition of the cell	8
i and type iii	8
in vero cells at	8
infected vero cells treated	8
the detection limit of	8
no significant difference between	8
vero e and huh	8
cells and incubated at	8
functional receptor for the	8
invasion rates of the	8
with vero e cells	8
is not essential for	8
as described in the	8
with or without myricetin	8
the s gene of	8
genes in vero cells	8
for h and then	8
in vero cells were	8
h of incubation at	8
hpi and hpi for	8
in cell culture and	8
isolated from kefir grains	8
is a functional receptor	8
virus entry and syncytia	8
entry and syncytia formation	8
an infectious cdna clone	8
spike protein of sars	8
that they have no	8
are shown in fig	8
for the first time	8
for h at rt	8
genome sequence of the	8
pbs for min at	8
of porcine epidemic diarrhoea	8
infected cells were treated	8
the virus life cycle	8
significantly lower than that	8
incubated with pedv at	8
declare that they have	8
there is an urgent	8
at the s s	8
was propagated in vero	7
the growth kinetics of	7
these results demonstrate that	7
and western blotting at	7
ic value of this	7
no effect on the	7
treatment of zikv infection	7
infected cells were detected	7
the virus inoculum was	7
at least three independent	7
genomic rna and sg	7
pretreated with the intracellular	7
in lung epithelial cells	7
in cells expressing s	7
of no and peroxynitrite	7
cell infection by pedv	7
to each well and	7
that of the parental	7
j cells were transfected	7
infected cells were fixed	7
of the virus in	7
cell lines were infected	7
on taxonomy of viruses	7
were significantly higher than	7
infected with chikv ls	7
the viral spike protein	7
vero cells with the	7
in a variety of	7
vero e cells infected	7
an urgent need to	7
a single amino acid	7
of vero cells on	7
proteolytic processing of the	7
of the virus to	7
they have no competing	7
on the expression of	7
cell cycle progression at	7
is consistent with the	7
as shown in figure	7
were incubated with pedv	7
have no competing interests	7
tcid ml at hpi	7
was used for the	7
with virus gd protein	7
test the invasion efficiency	7
the cell monolayers were	7
within the helicase protein	7
novel coronavirus in patients	7
with gds or gds	7
is an urgent need	7
the replication of the	7
of the icpc a	7
observed in cells expressing	7
is known to be	7
and western blotting assay	7
rna was extracted using	7
in a co atmosphere	7
days two and four	7
international committee on taxonomy	7
well plates until confluence	7
functional characterization of the	7
to the wt sequence	7
as well as to	7
was performed using the	7
stage of zikv infection	7
in the s region	7
mutations in the s	7
epidemic diarrhea virus strain	7
of vesicular stomatitis virus	7
in both vero and	7
a high level of	7
of the expression of	7
at days two and	7
results suggest that the	7
in contrast to the	7
with pfu of rzikv	7
more than of the	7
can be used for	7
and porcine epidemic diarrhea	7
a novel coronavirus in	7
expression of the viral	7
in addition to the	7
the identity of the	7
compared to the control	7
the replication of sars	7
similar to those of	7
cells in the absence	7
titer was determined by	7
a wide range of	7
was carried out using	7
in both cell types	7
identification and characterization of	7
as severe acute respiratory	7
used to infect vero	7
the neutralization capacity of	7
coronavirus in patients with	7
with acute respiratory distress	7
wild type and ribv	7
for min and then	7
confluent monolayers of vero	7
at nm was measured	7
mode of action of	7
the absence of trypsin	7
higher than that in	7
cell death during infection	7
as well as in	7
entry into host cells	7
significant difference between the	7
of these cell lines	7
data are shown as	7
during its propagation in	7
monolayers of vero e	7
was detected in the	7
play an important role	7
the antiviral efficacy of	7
in severe acute respiratory	7
in vero cells as	7
were compared to those	7
which is consistent with	7
in both cell lines	7
the percentage of infected	7
viral titers in the	7
to vero e cells	7
in vero cells was	7
moi of pfu cell	7
associated with acute respiratory	7
the g c mutation	7
vero cells were pretreated	7
new variants of porcine	7
the effects of the	7
with paraformaldehyde in pbs	7
cell fusion activities of	7
to test the invasion	7
h before virus infection	7
cells were then washed	7
observed in vero cells	7
analysis of the growth	7
human airway epithelial cells	7
committee on taxonomy of	7
complete genome sequence of	7
lines were infected with	7
the presence of the	7
reverse genetics system for	7
of the intracellular extracts	7
the absence of virus	7
cells were resuspended in	7
early stages of infection	7
cells were seeded at	7
in the spike protein	7
then infected with pedv	7
the early stage of	7
in different cell lines	7
alone or in combination	7
pi k akt signaling	7
plates were incubated at	7
for vero e cells	7
we found that the	7
well plates at a	7
titers were determined by	7
ns a a v	7
on the plasma membrane	7
the s and s	7
test was used to	7
in vitro prophylactic and	7
entry into vero cells	7
the genome of the	7
were used at a	7
the viability of the	7
vero e cell culture	7
between the gds and	7
vitro prophylactic and therapeutic	7
in the cytoplasm of	7
with uncleaved s protein	7
no conflict of interest	7
vitro prophylactic effects of	7
the culture supernatants were	7
the replication cycle of	7
in this study were	7
in the vero e	7
of viral rna in	7
supplemented with fbs and	6
the nucleotide sequence of	6
were infected with chikv	6
the arg residue to	6
experiment with two replicates	6
incubated with gds or	6
studies have suggested that	6
sequence of the sars	6
herpes simplex virus glycoprotein	6
the etiological agent of	6
of the virus was	6
the authors declare no	6
s and s subunits	6
in vero cells with	6
various time points post	6
water was used as	6
activity of the s	6
in the coinfection group	6
was used in the	6
species severe acute respiratory	6
total rna extracted from	6
the species severe acute	6
respiratory syndrome coronavirus infection	6
tissue culture infectious dose	6
at h after transfection	6
the addition of trypsin	6
antiviral activity against sars	6
this is the first	6
epidermal growth factor receptor	6
the institutional animal care	6
cov infection in vero	6
infectious bronchitis virus a	6
plays an important role	6
sirna and infected with	6
fit to a variable	6
virus is also shown	6
lungs and spinal cord	6
approximately and in the	6
performed as described previously	6
both in vitro and	6
activities of these s	6
were removed by washing	6
of these s constructs	6
in the beaudette strain	6
van dinten et al	6
with an equal volume	6
caused by a novel	6
each well and incubated	6
in an atmosphere of	6
in vero cells but	6
of the international committee	6
in the viral genome	6
samples were collected from	6
entry into vero e	6
western blot analysis of	6
absence of virus is	6
virus entry at the	6
the viral life cycle	6
three times with pbst	6
virus titers in supernatants	6
the genome sequence of	6
were fixed at hpi	6
cells were detected by	6
binding and entry process	6
were purchased from the	6
involved in pedv entry	6
of mx and isg	6
the footpad with pfu	6
a reverse genetics system	6
times with pbs and	6
of viral rna synthesis	6
a new porcine enteric	6
vero cells were transfected	6
protein has been shown	6
in vero cells is	6
the results of this	6
was also observed in	6
j cells were pre	6
inhibit the replication of	6
that the vero e	6
distilled water was used	6
by the institutional animal	6
to increasing concentrations of	6
with paraformaldehyde for min	6
of the virus and	6
were performed in triplicate	6
of the ns a	6
vero e cells at	6
reverse transcription was performed	6
the mutant virus was	6
of the severe acute	6
in group were inoculated	6
were mapped to the	6
the treatment of zikv	6
new porcine enteric coronavirus	6
a potent inhibitor of	6
hu carrying mutations in	6
inhibition was determined relative	6
p p p p	6
respiratory syndrome coronavirus in	6
cells exposed to increasing	6
were carried out in	6
of the virus life	6
of virus is also	6
fusion activities of these	6
of the sars vaccine	6
bars represent standard deviations	6
to test this hypothesis	6
experiments were performed in	6
with the exception of	6
at later time points	6
footpad with pfu of	6
two replicates is shown	6
variable response curve from	6
cell viability was measured	6
of intracellular viral rna	6
and cells were washed	6
we did not observe	6
cut fragment of p	6
was significantly higher than	6
stained with crystal violet	6
in one of the	6
transducer and activator of	6
virus inoculum was removed	6
with diarrhea in swine	6
of the vero cells	6
food and drug administration	6
nucleotide sequence of the	6
hepatitis c virus infection	6
of the antiviral activity	6
viability of vero cells	6
passaged in vero cells	6
analysis was performed using	6
confirmed by western blotting	6
from one representative experiment	6
drugs in the absence	6
did not affect the	6
birds in each group	6
of vero cells was	6
levels of zikv rna	6
percentage of infected cells	6
a variable response curve	6
were similar to those	6
be involved in the	6
our data indicated that	6
rna and sg mrna	6
human immunodeficiency virus type	6
and incubated with gds	6
cytotoxic effect on vero	6
also been shown to	6
for the presence of	6
then incubated for h	6
of at least three	6
e cells infected with	6
of avian infectious bronchitis	6
isolation of a novel	6
and infected with junv	6
c for h then	6
the three cell lines	6
single amino acid substitution	6
has been reported that	6
of the two viruses	6
were then incubated with	6
like particle associated with	6
after h of adsorption	6
least three independent experiments	6
against porcine epidemic diarrhea	6
approved by the institutional	6
infected cells treated with	6
possessed an in vitro	6
inhibition of viral rna	6
by severe acute respiratory	6
were orally inoculated with	6
of hepatitis c virus	6
replication of sabin poliovirus	6
cells were maintained at	6
of the parental virus	6
of the cell lines	6
cleavage of the s	6
induces apoptosis in vero	6
female balb c mice	6
were approved by the	6
immunostaining using the pan	6
vero cell monolayers in	6
total rna was isolated	6
in the icpc a	6
particle associated with diarrhea	6
prrsv and pedv were	6
the cell lysates were	6
but there was no	6
titrated by plaque assay	6
the a v mutation	6
all cell lines were	6
exposed to increasing concentrations	6
containing trypsin for h	6
it has been reported	6
to be able to	6
virus in porcine cell	6
titers in the supernatant	6
in a volume of	6
were infected with sars	6
cdna clone of mv	6
cells as well as	6
for prrsv and pedv	6
followed by cycles of	6
cells were then incubated	6
vero cells in the	6
statistical analysis was performed	6
increasing concentrations of drugs	6
institute of infectious diseases	6
fixed with paraformaldehyde for	6
with a moi of	6
has also been reported	6
for h prior to	6
a a v mutation	6
a moi of for	6
sequence analysis of the	6
were treated with different	6
clarified by centrifugation at	6
at a dosage of	6
isolation of porcine epidemic	6
coronavirus spike protein by	6
inhibits the replication of	6
to be involved in	6
and titrated by plaque	6
with two replicates is	6
from vero cells infected	6
cells as described previously	6
been shown to induce	6
to a variable response	6
the expression of a	6
and the supernatants were	6
body weight loss and	6
introduced into vero cells	6
the spike glycoprotein of	6
terminal domain of the	6
can be used to	6
gds or gds strains	6
our results showed that	6
in cells treated with	6
pcr was performed using	6
the s domain of	6
removed and the cells	6
in the context of	6
with or without blfp	6
of drugs in the	6
in the form of	6
log s gene copies	6
by western blot analysis	6
response curve from one	6
antiviral activity of the	6
cells were fixed at	6
has the potential to	6
virus gd protein to	6
s region of the	6
of egfr pi k	6
fragment of p tl	6
from three independent experiments	6
antiviral activity of potential	6
of the mock group	6
concentrations of peptide h	6
were obtained from the	6
were infected with zikv	6
results confirmed that the	6
signal transducer and activator	6
expression levels of the	6
dephosphorylation of stat in	6
group were inoculated with	6
is shown in figure	6
moi of for h	6
infected or infected with	6
the presence of mm	6
and activator of transcription	6
for porcine epidemic diarrhea	6
of a variety of	6
cells infected with ribv	6
for the generation of	6
s rrna and rpoa	6
the presence of a	6
added to vero cells	6
the blfp crude extract	6
using graphpad prism software	6
concentrations of drugs in	6
isolates of sars coronavirus	6
of the arg residue	6
in vero cell culture	6
in this study are	6
was similar to that	6
in dmem supplemented with	6
p in vero cells	6
s gene copies ml	6
infected vero cells with	6
the cells three times	6
from cells transfected with	6
cells were fixed in	6
dmem supplemented with fbs	6
mutation of the arg	6
supernatants were harvested at	6
that myricetin may interact	6
there were no significant	6
activity of peptide h	6
conjugated to alexa fluor	6
significant difference between groups	6
the murine coronavirus spike	6
did not show any	6
be explained by the	6
as early as hpi	5
the fusion activity of	5
different concentrations of each	5
entry at the plasma	5
the centers for disease	5
followed by staining with	5
mechanisms of action of	5
effectively inhibit the recently	5
which was significantly lower	5
and chloroquine effectively inhibit	5
as a receptor for	5
peripheral blood mononuclear cells	5
bands were visualized using	5
after the second transfection	5
is known about the	5
the fact that the	5
expression in vero cells	5
of pfu per cell	5
and clarified by centrifugation	5
respiratory syndrome coronavirus replication	5
cell lines were maintained	5
plaque assay in vero	5
by a novel coronavirus	5
by plaque assay in	5
in order to determine	5
depends on ace and	5
virus titers were determined	5
signal transduction in sars	5
entry depends on ace	5
belongs to the genus	5
diarrhea virus spike protein	5
of mm ammonium chloride	5
effects against pedv in	5
the american type culture	5
in vero and huh	5
it was reported that	5
it will be interesting	5
the cells were treated	5
to inoculate a cm	5
nucleotides to of the	5
pcr products were purified	5
treatment of vero cells	5
fig a and b	5
epidemic diarrhea virus spike	5
dmso was used as	5
cell fusion activity by	5
coronavirus induces apoptosis in	5
the plaque reduction assay	5
to the parental pedvpt	5
we also found that	5
of pigs with a	5
were detected by immunocytochemistry	5
cells pretreated with the	5
of harringtonine for h	5
were added to the	5
viral rna mini kit	5
in porcine cell cultures	5
is lethal to the	5
cells infected with sars	5
a line of african	5
to the cell surface	5
viral titers were determined	5
extracted from the infected	5
data were analyzed by	5
blotting at hpi and	5
mutant virus from p	5
of the mutant rzikv	5
the lab isolates were	5
at days after challenge	5
to further confirm the	5
infection of vero e	5
the specificity of the	5
were significantly more attenuated	5
turkey with confirmed covid	5
is blocked by a	5
drugs were used at	5
significance was analyzed from	5
of action of ribavirin	5
to the research community	5
the viral envelope with	5
the recombinant ibvs were	5
cells treated with the	5
and stained with anti	5
in dmem containing fbs	5
swine pedv positive serum	5
sars coronavirus infection in	5
as a loading control	5
were fixed with formaldehyde	5
analyzed by western blotting	5
to cells and incubated	5
strains were and in	5
plaque assays were performed	5
patient in turkey with	5
signaling pathways are activated	5
facilitate the development of	5
for replication in cell	5
and is blocked by	5
or gds strains for	5
natural science foundation of	5
the s s region	5
the data presented as	5
on cell infection by	5
after days of incubation	5
chloroquine effectively inhibit the	5
were introduced into vero	5
previous studies have shown	5
cell entry depends on	5
blocks cell cycle progression	5
of each viral stock	5
by interfering with the	5
a or icpc a	5
used to determine the	5
national institute of infectious	5
read and approved the	5
to the results of	5
may be involved in	5
were removed and the	5
at each time point	5
functions within the helicase	5
treated and untreated cells	5
via the cyclin d	5
vero cells were co	5
extracted from vero cells	5
mem containing trypsin for	5
pedv s protein was	5
used to inoculate a	5
cell substrates for the	5
a better understanding of	5
vero cells transfected with	5
the mode of action	5
a variety of viruses	5
an moi of pfu	5
followed by incubation with	5
the most commonly used	5
and approved the final	5
cells treated with or	5
on the left indicate	5
in vitro assembly of	5
the e glycoprotein of	5
the number of cells	5
and virus titers were	5
phosphorylation and activation of	5
expression of s protein	5
the s rrna and	5
piglets in group were	5
were collected from the	5
was analyzed from t	5
data presented as mean	5
was used to compare	5
from the ministry of	5
the infection of pedv	5
as well as other	5
of this ns a	5
the wt sequence during	5
during the course of	5
plates were incubated for	5
an atmosphere of co	5
pcr was carried out	5
wt sequence during its	5
and the presence of	5
of the subgenomic mrna	5
of infected vero e	5
of s gene mutants	5
end of the genome	5
up to days post	5
the effect of trypsin	5
cov in vero e	5
cleavage of the e	5
in vitro prophylactic effect	5
the groups treated with	5
that pedv entry followed	5
binding site in the	5
groups of five female	5
dna and rna viruses	5
and western blotting analysis	5
vero e cells by	5
were propagated in vero	5
virus in cultured cells	5
fixed at hpi and	5
clinically proven protease inhibitor	5
was allowed to proceed	5
then washed three times	5
of the s gene	5
tissue culture supernatants were	5
a patient in turkey	5
the s subunit of	5
infection of pigs with	5
factors that influence ebola	5
tmprss and is blocked	5
to of the viral	5
no significant differences in	5
blfp crude extract were	5
of transmissible gastroenteritis coronavirus	5
viral titer in the	5
and immunostaining using the	5
treated with proteinase k	5
allowed to proceed for	5
the amount of virus	5
than those of the	5
vero e cells to	5
supernatants were determined by	5
we found that pedv	5
cellulose in mem containing	5
have been used to	5
of the ibv genome	5
in vitro transcribed rna	5
diarrhea virus in porcine	5
the culture of vero	5
was considered to be	5
serial dilutions of the	5
particular ic value of	5
were monitored daily for	5
adapted ibv beaudette strain	5
the recovery of infectious	5
that influence ebola antiviral	5
fold serial dilution of	5
at day after infection	5
together with the n	5
h and incubated with	5
the feasibility of generating	5
was used to detect	5
spike protein is a	5
expressed as the mean	5
possessed in vitro prophylactic	5
the vero jcrb cell	5
coronavirus associated with human	5
time course analysis of	5
a duplicated subgenomic promoter	5
these results indicate that	5
jejunum and ileum of	5
c bl j mice	5
the entire genome of	5
in hek t cells	5
linear fit to a	5
ebola antiviral activities in	5
of viral protein expression	5
twice with sterile pbs	5
on the cell surface	5
member of the genus	5
control cells containing the	5
institute of biomedical sciences	5
cell lines such as	5
cotton rats in groups	5
virus in vero cells	5
did not observe a	5
cycle progression at g	5
the qiaamp viral rna	5
it should be noted	5
from a patient with	5
with the n transcripts	5
identity of the rescued	5
of the parental pedvpt	5
harvested at h post	5
carried out in the	5
stages of viral infection	5
the recently emerged novel	5
we were able to	5
with respect to the	5
of african swine fever	5
as a positive control	5
that the inhibition of	5
apoptosis induced by sars	5
compared with the zikv	5
us porcine epidemic diarrhea	5
with methylcellulose in dmem	5
pfu ml at hpi	5
to the method described	5
the drug was added	5
of the e glycoprotein	5
confluent vero cells in	5
science foundation of china	5
these data indicate that	5
its propagation in vero	5
the membrane was incubated	5
detection limit of the	5
cdna clone of the	5
supernatants were collected and	5
the virus titer in	5
the heptad repeat region	5
significantly higher than that	5
acidophilus cmul and l	5
removed and the monolayers	5
be attributed to the	5
a wide variety of	5
in turkey with confirmed	5
treated with lm snap	5
by grants from the	5
vero cells were prepared	5
the cyclin d prb	5
treated with the recombinant	5
conformational changes in the	5
were found to be	5
was determined by a	5
cells but not in	5
the treatment of covid	5
qiaamp viral rna mini	5
with increasing concentrations of	5
into vero e cells	5
order genetic assembly system	5
to determine whether the	5
provided in supplementary file	5
the seven recombinant ibvs	5
assay was performed to	5
was used at a	5
with a new porcine	5
we are grateful to	5
mutation in a domain	5
to further investigate the	5
a clinically proven protease	5
standard deviations of the	5
in a line of	5
of the bacterial intracellular	5
was measured using a	5
growth properties of the	5
supported by grants from	5
plates at a density	5
were incubated for min	5
and tmprss and is	5
for h followed by	5
hsv binding and entry	5
numbers on the left	5
drugs for treatment of	5
icpc a or icpc	5
cellular egfr pi k	5
of an antisense oligonucleotide	5
infectious bronchitis virus in	5
the infectious bronchitis virus	5
the infected vero cells	5
e glycoprotein of murine	5
h prior to infection	5
progression at g g	5
vero cells pretreated with	5
vero e and llcmk	5
our understanding of the	5
the particular ic value	5
results were confirmed by	5
were used to infect	5
of an avian coronavirus	5
of human coronavirus e	5
cyclin d prb pathway	5
at room temperature with	5
pigs with a new	5
antiviral activities in cell	5
from the american type	5
protein to interfere with	5
are listed in table	5
of icpc a was	5
the viral rna was	5
could be due to	5
at rt for h	5
the bacterial intracellular extracts	5
patients recovered from covid	5
the bac cdna clone	5
at room temperature and	5
the generation of a	5
and u cell lines	5
inhibit the recently emerged	5
and naming it sars	5
genomic characterization of a	5
of the infected cells	5
when the drug was	5
concentrations of each compound	5
significant difference in the	5
the activation of egfr	5
was approved by the	5
all animal experiments were	5
little is known about	5
of the viral life	5
further characterization of the	5
at this time point	5
transcripts containing the g	5
antiviral activity in vitro	5
antiviral drug candidates against	5
were then overlaid with	5
the viability of vero	5
the mechanism of action	5
and then shifted to	5
nucleotide sequences of the	5
stocks were prepared by	5
a broad range of	5
of west nile virus	5
the detection of icpc	5
resuspended in ml of	5
results were expressed as	5
the detection of the	5
higher than those of	5
with porcine epidemic diarrhea	5
was added at the	5
of the vero cell	5
in a similar manner	5
was carried out with	5
with the zikv group	5
weight loss and survival	5
cells infected with chikv	5
human respiratory disease in	5
recently emerged novel coronavirus	5
ace and tmprss and	5
the entry mechanism of	5
in vitro inhibition of	5
well and incubated at	5
western blotting at hpi	5
demonstrate the feasibility of	5
added to cells and	5
infected with pedv at	5
the egfp reporter gene	5
in the pathogenesis of	5
in accordance with the	5
and foetal calf serum	5
and hepatitis c virus	5
invasion rates of pedv	5
gd protein to interfere	5
allowed to incubate for	5
in mem containing trypsin	5
removed and cells were	5
was not observed in	5
of a number of	5
swine acute diarrhea syndrome	5
into the host cell	5
inoculated with ml of	5
these results confirm that	5
h after the first	5
of the cell monolayer	5
the vero cell monolayers	5
remdesivir and chloroquine effectively	5
the consensus sequence of	5
adsorption and membrane fusion	5
were incubated at room	5
methyl cellulose in mem	5
treatment of cells with	5
assay was performed as	5
a high degree of	5
an essential role in	5
approved for clinical use	5
plaque assay and immunostaining	5
in cells electroporated with	5
in a domain with	5
the plasma membrane of	5
the pcr products were	5
of virus to the	5
stock was determined by	5
will be interesting to	5
and incubated for min	5
groups treated with the	5
drug candidates against sars	5
tcid ml of the	5
new coronavirus associated with	5
log rna copies ml	5
at different stages of	5
the oxygen atom of	5
after the first transfection	5
the pedv blfp group	5
gds strains for h	5
those of the parental	5
rrna and rpoa gene	5
were treated with or	5
myricetin may block hsv	5
the sialic acid binding	5
be used for the	5
were fixed with acetone	5
the parental vaccine strain	5
these results demonstrate the	5
pedv entry into vero	5
substrates for the production	5
were analyzed using the	5
error bars represent the	5
and mechanism of action	5
were added to each	5
effect of trypsin on	5
rna was detected in	5
of middle east respiratory	5
were and in the	5
with human respiratory disease	5
authors declare no conflict	5
were added to cells	5
in the perinuclear region	5
between the two cell	5
vero cells as described	5
influence ebola antiviral activities	5
of the pedv s	5
statistical significance was analyzed	5
paraformaldehyde in pbs for	5
cell lines for the	5
in vitro models of	5
the difference in the	5
virus infection in vitro	5
was kindly provided by	5
the induction of cell	5
wild type and mutant	5
the cell monolayer was	5
morbidity and mortality rates	5
declare no conflict of	5
the molecular biology of	5
sam binding site in	5
in the pedv blfp	5
for h and incubated	5
at g g phase	5
with various concentrations of	5
blocked by a clinically	5
the number of foci	5
study of the antiviral	5
results of this study	5
the icpc a and	5
and the virus titer	5
was washed three times	5
in a co incubator	5
the virus titer was	5
g c mutant transcripts	5
on ace and tmprss	5
assay and immunostaining using	5
commercially available antiviral agents	5
the ministry of health	5
in different cell types	5
significantly higher than those	5
infected with the recombinant	5
was removed and cells	5
test compared with the	5
it is known that	5
as the primary antibody	5
in order to evaluate	5
was carried out in	5
and rpoa gene sequence	5
sequence during its propagation	5
were found in the	5
peptide h and pedv	5
would like to thank	5
is a receptor for	5
or absence of ribavirin	5
detection of icpc a	5
added into each well	5
on the basis of	5
were no significant differences	5
to the end of	5
cultured in the presence	5
infection of pedv icpc	5
gds strains were and	5
the clinical management of	5
infected with the mutant	5
jnk and pi k	5
infectivity of the in	5
time points post infection	5
according to the method	5
porcine transmissible gastroenteritis virus	5
replication in cell culture	5
virus into vero cells	5
associated with human respiratory	5
and ck ch ldl	5
the rescued virus was	5
these s constructs are	5
a new coronavirus associated	5
and vero cell lines	5
crude extract were determined	5
or without blfp crude	5
performed as described above	5
of porcine reproductive and	5
after washing twice with	5
were cultured overnight in	5
and the plaques were	5
are thought to be	5
ncov and naming it	5
were separated by sds	5
might be due to	5
and the data presented	5
was added to vero	5
respiratory disease in china	5
by a clinically proven	5
in the heptad repeat	5
and swine pedv positive	5
difference between the gds	4
the hpi time point	4
porcine epidemic diarrhea coronavirus	4
treated with various concentrations	4
in mammalian cell lines	4
is a class i	4
for the study of	4
avian infectious bronchitis coronavirus	4
terminal region of the	4
results indicated that pedv	4
r k and r	4
inoculum was removed followed	4
the amount of gd	4
for h in the	4
fusion and syncytia formation	4
a one sample t	4
vero cell monolayers were	4
cell cultures and mice	4
step growth curves of	4
respiratory syncytial virus infection	4
new coronavirus of probable	4
in the release of	4
a new coronavirus of	4
coronavirus of probable bat	4
the expression of s	4
icpc a replicated to	4
the different cell lines	4
of vero cells vol	4
detection and quantification of	4
and hela cell lines	4
that the differences between	4
grew to high titer	4
at c followed by	4
diarrhea virus into vero	4
results demonstrate that the	4
control by the humoral	4
k akt signaling pathway	4
amplification of cdna ends	4
virions interacted with vero	4
from a vero cell	4
play important roles in	4
mapk and its downstream	4
in this report we	4
murine coronavirus spike protein	4
onset of clinical signs	4
for min to remove	4
to the s protein	4
the percentage inhibition was	4
for the clinical management	4
lethal to the coronavirus	4
transfected with the mutant	4
seeded with vero e	4
reduction of the viral	4
vero cells were incubated	4
cell spread of hsv	4
the reason for the	4
newly discovered coronavirus associated	4
an increase in the	4
with prrsv or pedv	4
porcine aminopeptidase n is	4
figure a and b	4
or in combination with	4
coronavirus of bat origin	4
that the intracellular extracts	4
inhibitory effects against pedv	4
prevention and control of	4
efficacy of m f	4
percentage inhibition was determined	4
outbreak associated with a	4
data were analyzed using	4
the type i interferon	4
viral replication in the	4
u ml penicillin and	4
based on the sequence	4
prepared by freezing thawing	4
representative of three separate	4
with an excess of	4
on ice for min	4
human suspension cell lines	4
to infection by sars	4
at the amino acid	4
during the early stages	4
a man with pneumonia	4
protein was detected by	4
characterization of a newly	4
replication of prrsv and	4
that pedv enters vero	4
using the mmessage mmachine	4
the central nervous system	4
used in the pcr	4
coronavirus severe acute respiratory	4
activity of toremifene citrate	4
as early as h	4
analyzed by western blot	4
with error bars represent	4
in vero cells by	4
with pedv at moi	4
is due to the	4
pedv entry relies on	4
and added to the	4
moi of pfu per	4
the number of viral	4
at concentrations ranging from	4
when data are compared	4
of the small intestine	4
vero cells in a	4
the time of infection	4
with sabin poliovirus type	4
by the world health	4
viral control by the	4
important role in the	4
than the gds strain	4
the presence of rounded	4
to the best of	4
p mapk and its	4
attenuated in vitro and	4
its interaction with the	4
associated with porcine epidemic	4
species as well as	4
identification of antiviral drug	4
d the vero cells	4
similar results were obtained	4
petrus jansen van vuren	4
by the humoral immune	4
the selection of the	4
from a man with	4
entire genome of each	4
values are representative of	4
myricetin may be able	4
it is necessary to	4
fold dilutions of the	4
in the vero and	4
the result shown is	4
mixture of two viruses	4
is a major receptor	4
biology and pathogenesis of	4
and isg genes in	4
were used as a	4
were incubated with rabbit	4
the large polymerase protein	4
to this article can	4
emergence of resistant viruses	4
time of infection or	4
knockdown of the expression	4
detected in vero cells	4
in human lung cells	4
entry mechanism of pedv	4
in the s s	4
the culture supernatant was	4
those of the mock	4
do not appear to	4
targeting the n gene	4
related coronavirus of bat	4
the genetic stability of	4
did not exhibit any	4
sabin poliovirus type in	4
to the fact that	4
simplex virus type infection	4
antigens targeting the s	4
replication in vero cells	4
are statistically significant when	4
represent standard deviations of	4
effects of chloroquine on	4
this article can be	4
that some of the	4
electroporated into vero cells	4
more insight into the	4
both icpc a and	4
to investigate the effect	4
inoculated with ped virus	4
a betacoronavirus in group	4
were more susceptible to	4
derived vero cell line	4
data are expressed as	4
to the coronavirus infectious	4
pedv enters vero cells	4
sequence derived from the	4
vero cells were first	4
virus titer was determined	4
duration of the experiment	4
the peak viral titer	4
like particles associated with	4
type and mutant viruses	4
amount of gd protein	4
member of the family	4
the use of a	4
on the consensus sequence	4
in vero jcrb cells	4
constitutive activation of stat	4
effect of ribavirin on	4
amount of viral rna	4
the phosphorylation of egfr	4
duplicated subgenomic promoter and	4
located in the c	4
crude extract of blfp	4
pellet was resuspended in	4
a bacterial artificial chromosome	4
rna was extracted and	4
were conducted based on	4
with the seven recombinant	4
as a percentage of	4
active in vero cells	4
cells were lysed with	4
apoptosis and necrosis have	4
frames encode nonessential functions	4
number of asterisks indicate	4
on porcine nidovirus replication	4
reading frames encode nonessential	4
were assessed with a	4
for severe acute respiratory	4
inhibited at a concentration	4
pedv rna was detected	4
at and h post	4
produced by escherichia coli	4
the differentially expressed proteins	4
intranasal and ocular routes	4
with phosphate buffered saline	4
based on the consensus	4
to the negative control	4
signaling pathways in sars	4
assess the antiviral activity	4
gene was amplified by	4
adsorbed to hela cells	4
both apoptosis and necrosis	4
blfp as a feed	4
the total amount of	4
was used in all	4
is a potent inhibitor	4
the development of new	4
the polybasic cleavage motif	4
titer in vero cells	4
each well and the	4
s protein by trypsin	4
virus produced in c	4
indicate that the differences	4
of p mapk and	4
compared the effects of	4
virus strains in the	4
as a feed additive	4
of influenza a viruses	4
and extracellular supernatants of	4
through direct interaction with	4
were treated with proteinase	4
the small intestine of	4
by the duplex rt	4
protection against challenge with	4
for the sars coronavirus	4
are expressed as the	4
to evaluate the viral	4
receptor for the sars	4
vero cells have been	4
cme to enter cells	4
has also been shown	4
the production of vaccines	4
conducted based on three	4
vero cells could be	4
well plates were inoculated	4
a major receptor for	4
with virus adsorption and	4
have been deposited in	4
well plates were washed	4
higher than that of	4
heptad repeat region of	4
in the nearby clusters	4
asterisks indicate that the	4
caused by an hku	4
cell death and survival	4
not human coronavirus nl	4
addition of guanosine to	4
for the recombinant ibvs	4
bars represent the mean	4
on the viability of	4
shown to induce apoptosis	4
interaction with virus gd	4
arg residue to a	4
used as the primary	4
of this graph is	4
has not yet been	4
nonstructural protein ns a	4
to block viral entry	4
nucleocapsid protein of sars	4
s s region of	4
differences were considered significant	4
analyzed by flow cytometry	4
man with pneumonia in	4
in virus growth medium	4
are shown as m	4
in virus entry and	4
in or near the	4
for the prevention of	4
the importance of this	4
jev production in huh	4
to replicate in these	4
hepatitis virus strain a	4
and middle east respiratory	4
design of the study	4
respiratory distress syndrome in	4
infection with chlamydia abortus	4
at c for min	4
binding of gd to	4
k and r l	4
revealed that pedv entry	4
passages in vero cells	4
in vitro using the	4
a final concentration of	4
viral stocks were prepared	4
entry step of sars	4
and used to infect	4
is possible that the	4
kit according to the	4
with a lethal dose	4
of the family coronaviridae	4
asterisks indicate significant difference	4
we compared the effects	4
productivity and susceptibility to	4
allergy and infectious diseases	4
at the beginning of	4
to determine the effect	4
the sequence of the	4
can enter cells through	4
to further assess the	4
cells were then overlaid	4
representative of the mean	4
an increase in virus	4
in blocking buffer for	4
mmessage mmachine t kit	4
not appear to be	4
cm tissue culture flask	4
against the pedv s	4
cell lines can support	4
reversion of the mutant	4
with the mutant virus	4
interacted with vero cells	4
mm nh cl or	4
after washing with pbs	4
and characterization of the	4
was performed according to	4
mutation at nucleotide position	4
the ministry of education	4
mixed infections with chlamydia	4
assay for detection and	4
can be found in	4
diarrhea virus strain pc	4
that acquisition of the	4
performed to determine the	4
membrane fusion and reduces	4
the causative agent of	4
and monitored for cpe	4
observed in cells infected	4
expression of viral proteins	4
was detected by qrt	4
and used to inoculate	4
statistical significance was determined	4
point mutation in the	4
we next sought to	4
a positively charged amino	4
shown is a representative	4
of the number of	4
targets of p mapk	4
of ck ch ldl	4
the mean from three	4
positively charged amino acid	4
vero e cells was	4
due to the low	4
the potential emergence of	4
a of the coronavirus	4
to further evaluate the	4
and the overexpression of	4
landscape of vero cells	4
to ensure that the	4
human and animal cells	4
were aligned using the	4
in vivo compared to	4
characterization of severe acute	4
virus to the cells	4
below the detection limit	4
by deka et al	4
or near the catalytic	4
the inhibitory activities of	4
g for min to	4
and the lack of	4
plaques were visualized by	4
in the spike glycoprotein	4
scrofa and chlorocebus sabaeus	4
mice infected with pfu	4
detection and titration of	4
cells and incubated for	4
activity against both hsv	4
in cultured cells and	4
vero cells via clathrin	4
for the duration of	4
prior to incubation with	4
most commonly used cell	4
as a measure of	4
in multiple cell types	4
determine the effect of	4
travel history to iran	4
using the unpaired t	4
the replication of porcine	4
are summarized in table	4
the most effective inhibitor	4
been tested in humans	4
observed in cells transfected	4
the expression level of	4
these two cell lines	4
from the first patient	4
cells transfected with z	4
coronavirus spike protein for	4
for the use of	4
spike protein for membrane	4
which belongs to the	4
was removed followed by	4
for membrane fusion and	4
on sv in tissue	4
the addition of guanosine	4
for the ratio of	4
absorbance at nm was	4
supplemented with fetal calf	4
was cloned by three	4
a discontinuous transcription mechanism	4
length transcripts containing the	4
plaque assay on vero	4
detected as early as	4
infected with a moi	4
have shown that the	4
the virus passage virus	4
activity of s protein	4
the unpaired t test	4
of cell death in	4
strain was more sensitive	4
were reported to have	4
the limit of detection	4
coronavirus replication in cell	4
the morbidity and mortality	4
at the institute of	4
that vero e cells	4
coronavirus in vero cells	4
the cells were harvested	4
were washed with phosphate	4
were grown in dmem	4
proteins were separated by	4
enters vero cells via	4
in the groups treated	4
related to the sars	4
cell lines infected with	4
rapid amplification of cdna	4
chikv ls and ls	4
were used in this	4
cells was determined using	4
a role in the	4
in both lungs and	4
necrosis have been observed	4
fold diluted virus stock	4
for the staining of	4
and found that the	4
titers in supernatants were	4
coronavirus associated with acute	4
of the amino acid	4
genetic stability of the	4
viral rna in the	4
calculated and expressed as	4
emergent porcine epidemic diarrhea	4
domain with undefined functions	4
were incubated for days	4
assay on vero cells	4
enhancement of the cell	4
for the induction of	4
of infected cells was	4
cases of severe acute	4
coronavirus mouse hepatitis virus	4
of lactic acid bacteria	4
that the replication of	4
nonessential functions for replication	4
a pneumonia outbreak associated	4
after the addition of	4
and activation of p	4
was able to induce	4
before washing with pbs	4
major receptor for the	4
of the m protein	4
based on the use	4
when it was added	4
the other cell lines	4
and cells were incubated	4
for h and were	4
isolation in cell culture	4
the correlation between the	4
of antiviral activity of	4
the ability of the	4
infectious bronchitis virus is	4
the coronavirus spike protein	4
that the recombinant ibvs	4
infectious virus from the	4
virus strain pc a	4
the production of virions	4
successful entry into cells	4
were washed once with	4
a cm tissue culture	4
tissue culture infective dose	4
genotyping of emergent porcine	4
by means of the	4
in the s gene	4
infection was allowed to	4
cells were then infected	4
pneumonia in saudi arabia	4
replication in cell cultures	4
of the novel human	4
were intranasally inoculated with	4
antiviral activity of ata	4
cultured in vero cells	4
prophylactic effects of the	4
authors read and approved	4
mesenteroides ypk against pedv	4
vero jcrb cell line	4
respiratory syndrome coronavirus entry	4
was significantly inhibited by	4
was quantified by real	4
could be inhibited by	4
for human coronavirus e	4
e cells were treated	4
in balb c mice	4
were significantly lower than	4
cathepsin l to infect	4
the number of infected	4
washed for three times	4
on the replication of	4
strain of porcine epidemic	4
the s protein gene	4
of infectious viral progeny	4
samples was determined by	4
the mutant virus from	4
compared using the unpaired	4
calpain and cathepsin inhibitors	4
culture of vero e	4
all of them survived	4
important insights into the	4
an inverted fluorescence microscope	4
incubated for min at	4
were approximately and in	4
the method described by	4
the igg and igm	4
the species rhinolophus bat	4
a percentage of the	4
cell culture supernatants were	4
of the mean from	4
the duration of the	4
but not human coronavirus	4
late stage of zikv	4
their effects on the	4
to induce apoptosis in	4
the s protein on	4
the mmessage mmachine t	4
which suggested that the	4
incubated for h with	4
guanosine to the ribavirin	4
obtained from the american	4
apoptosis via a caspase	4
and iu ml for	4
the concentration of the	4
was supported by grants	4
with pneumonia in saudi	4
of the plasma membrane	4
the internalization rates of	4
protein similar to p	4
of the synthetic viruses	4
of the negative strand	4
of stat in sars	4
has been reported to	4
strains in the united	4
membrane fusion activity of	4
for the propagation of	4
the phosphorylation and activation	4
both levels of zikv	4
to the presence of	4
between virus and host	4
then the amount of	4
with the plasma membrane	4
of the antisense oligonucleotide	4
visualized by staining with	4
viral protein expression in	4
encode nonessential functions for	4
sv in tissue culture	4
viruses at a multiplicity	4
in agreement with these	4
pedv entry followed clathrin	4
and analyzed by flow	4
and human leukocyte interferon	4
gds strain was more	4
it was observed that	4
in vero cells for	4
the second passage of	4
vitro prophylactic effect against	4
in the blfp crude	4
the enzymatic activities of	4
carried out using the	4
that of the single	4
either mm nh cl	4
then washed with pbs	4
mouse hepatitis virus strain	4
genomic and subgenomic rna	4
type i interferon in	4
prophylactic effect against pedv	4
respiratory syndrome coronavirus by	4
the genomic and subgenomic	4
entry of mers pseudovirions	4
by western blot with	4
virus binding and entry	4
on the use of	4
pck and cho cells	4
of antiviral drug candidates	4
room temperature for min	4
bronchitis virus a polyprotein	4
may be related to	4
plays important roles in	4
with fetal calf serum	4
was performed using a	4
staining with crystal violet	4
samples were incubated for	4
zika virus infection in	4
to hepatitis c virus	4
sus scrofa and chlorocebus	4
porcine intestinal epithelial cells	4
to further analyze the	4
all authors read and	4
associated with a new	4
to the ribavirin treatment	4
were then washed with	4
of wild type and	4
used in the treatment	4
in order to test	4
mice infected with the	4
of guanosine to the	4
treatment of vero e	4
value of this graph	4
of probable bat origin	4
the percentage of ebov	4
used for the production	4
polyclonal antibody to the	4
cells were cultured overnight	4
pneumonia outbreak associated with	4
of the african green	4
simplex virus glycoprotein d	4
at the same position	4
infected with junv at	4
were randomly assigned to	4
virus infection and the	4
was assigned values of	4
been shown to have	4
acute respiratory syndrome a	4
the development of vaccine	4
and hamster cell lines	4
could be attributed to	4
and pi k akt	4
the activity of cyclin	4
the outer capsid layer	4
removed followed by three	4
the infectivity of ibv	4
in vitro inhibitory activity	4
inhibitory activity of peptide	4
were performed using the	4
no significant difference in	4
by staining with crystal	4
of the s subunit	4
transmission of zika virus	4
japanese encephalitis reporter virus	4
the cells were stained	4
inhibitor of chikv replication	4
both lungs and spinal	4
plates were inoculated with	4
that the compound may	4
and then permeabilized with	4
no and peroxynitrite on	4
monolayer of vero e	4
induced cell death than	4
the humoral immune response	4
pensaert and de bouck	4
were inoculated with pfu	4
statistically significant when data	4
were used to amplify	4
was effective against chikv	4
and approved by the	4
in the cell culture	4
viral titer was determined	4
observed for other alphaviruses	4
cells were compared to	4
changes in the viral	4
cell culture model of	4
cell fusion and syncytia	4
containing the g c	4
it has been shown	4
approved the final manuscript	4
the nucleocapsid protein of	4
higher cell metabolic activity	4
with a new coronavirus	4
a part of the	4
viral protein expression was	4
from cells treated with	4
was used to identify	4
were fixed with methanol	4
and the development of	4
that the isolated virus	4
error of the mean	4
of virus yield inhibition	4
of emergent porcine epidemic	4
a result of the	4
which recognizes residues to	4
and infected with the	4
with the addition of	4
been used to treat	4
belong to the species	4
the majority of the	4
isg genes in vero	4
buffer for h at	4
probably due to the	4
passaged sabin poliovirus type	4
the fact that they	4
are available upon request	4
cells for h at	4
was shown to be	4
in supernatants were determined	4
g g phase via	4
of a newly discovered	4
death during infection by	4
challenge with the parental	4
in all groups were	4
authors declare that there	4
fusion and reduces viral	4
virus adsorption and membrane	4
swollen head syndrome in	4
to vero cell monolayers	4
enhanced the infectivity of	4
the avian coronavirus infectious	4
distress syndrome in humans	4
are compared using the	4
the plaques were counted	4
region of the s	4
in the ns a	4
a crucial role in	4
of prrsv or pedv	4
with undefined functions within	4
the ck ch ldl	4
utilizes cathepsin l to	4
bronchitis virus in cultured	4
a domain with undefined	4
to various types of	4
used as a template	4
we found that a	4
and the number of	4
the effects of chloroquine	4
genome landscape of vero	4
the vero cell culture	4
result shown is a	4
the mixture of two	4
of vero cells is	4
later stages of infection	4
of the genome of	4
based on three replicates	4
preliminary step for cancer	4
and genotyping of emergent	4
and those less than	4
the upper and lower	4
a region of the	4
were incubated at c	4
s domain of mers	4
indicate significant difference between	4
compared to infected cells	4
taiwan porcine epidemic diarrhea	4
the number of plaques	4
pellets were resuspended in	4
the n transcripts were	4
inoculate a cm tissue	4
was reported to be	4
downstream targets of p	4
have been observed in	4
the s protein was	4
we set out to	4
grown on glass coverslips	4
with the mutant transcripts	4
lungs were collected for	4
antibody conjugated to alexa	4
as shown in table	4
g a grew to	4
were added into each	4
swine acute diarrhoea syndrome	4
were harvested at h	4
in a mouse model	4
for h before infection	4
inactivated fetal bovine serum	4
adsorbed but not internalized	4
the novel human betacoronavirus	4
vero cells were pre	4
calculated based on the	4
the genome landscape of	4
a newly discovered coronavirus	4
virus titers were calculated	4
s and s species	4
is a representative of	4
the nucleotide sequences of	4
were prepared by freezing	4
of pedv n proteins	4
the same results were	4
clone was fully stable	4
and akt proteins in	4
compared to the non	4
or infected with prrsv	4
the gds strain was	4
than of the cells	4
the annotations in the	4
infection through direct interaction	4
carried out using a	4
a monolayer of vero	4
kda protein similar to	4
effects on the infection	4
did not differ significantly	4
the absence of serum	4
suggested that the compound	4
levels of intracellular viral	4
cell monolayers were washed	4
leader sequence derived from	4
ypk possessed in vitro	4
levels of viral rna	4
antiviral effect of ribavirin	4
diarrhea virus strains in	4
is a determinant of	4
that the s protein	4
novel coronavirus from a	4
and the monolayers were	4
and the supernatant was	4
antibody to the sars	4
of asterisks indicate significant	4
were expressed as the	4
drug was added at	4
of an african green	4
activity of blfp crude	4
the levels of phosphorylated	4
extract were determined by	4
studies on sv in	4
the replication of prrsv	4
and necrosis have been	4
the supernatants were harvested	4
that myricetin possessed anti	4
in the supernatants of	4
with the vero cell	4
of the nucleocapsid protein	4
l to infect ace	4
of action of chloroquine	4
cell infection by tgev	4
near the catalytic centers	4
in the design of	4
inhibitors of cathepsin l	4
were then infected with	4
evaluated by western blot	4
gene sequences of the	4
a limited number of	4
were confirmed by western	4
recovery of infectious virus	4
a dosage of mg	4
with pbs and then	4
ml per well of	4
performed according to the	4
produced in vero cells	4
infectious virus could be	4
the bac plasmid pbelobac	4
a porcine epidemic diarrhea	4
enzyme is a functional	4
during serial passages in	4
of pedv in vero	4
and the infected cells	4
for mouse hepatitis virus	4
h prior to incubation	4
two and four after	4
potential emergence of resistant	4
direct interaction with virus	4
data are compared using	4
hepatitis c virus rna	4
infectious virus was recovered	4
icpc a and coinfection	4
of type i interferons	4
rpoa gene sequence analysis	4
the a protein of	4
intracellular viral rna levels	4
were washed twice in	4
epidemic diarrhea virus into	4
this graph is indicated	4
infectious bronchitis virus and	4
significant when data are	4
into each well and	4
blfp crude extract was	4
of sars coronavirus infection	4
growth kinetics of sars	4
positive for both viruses	4
apoptosis was observed in	4
cell lines that are	4