trigram

This is a table of type trigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

trigram	frequency
vero e cells	307
in vero cells	289
severe acute respiratory	219
acute respiratory syndrome	214
vero cells were	187
porcine epidemic diarrhea	178
the presence of	156
the cells were	154
epidemic diarrhea virus	144
respiratory syndrome coronavirus	132
were infected with	130
in the presence	114
in vero e	106
in this study	106
of vero cells	100
as well as	98
cells were infected	90
according to the	89
of the virus	88
of the viral	82
for h at	82
the s protein	82
infectious bronchitis virus	81
infected vero cells	80
antiviral activity of	75
the expression of	75
cells infected with	73
data not shown	72
herpes simplex virus	70
based on the	69
compared to the	69
cells were washed	69
vero cells and	67
of severe acute	66
of porcine epidemic	66
at room temperature	65
to determine the	64
the number of	64
as described above	64
cells were fixed	63
was used to	61
of the s	60
the treatment of	60
as shown in	59
for min at	59
of the sars	59
we found that	58
the effect of	58
in order to	58
shown in fig	57
was added to	56
were treated with	54
the vero cell	54
cells were incubated	54
the development of	54
in cell culture	53
the vero cells	53
the present study	53
as described previously	53
were incubated with	52
an moi of	52
the replication of	50
infected cells were	50
analysis of the	50
infected vero e	50
was used as	50
were inoculated with	49
of icpc a	49
in the vero	48
the production of	48
was determined by	48
cells were treated	47
c for h	47
in vitro and	46
the absence of	46
one of the	46
due to the	46
of a novel	46
african green monkey	46
on vero e	45
in the s	45
and incubated at	45
rna was extracted	45
infected with the	44
hepatitis c virus	44
of the cell	44
cells treated with	44
fetal bovine serum	43
blfp crude extract	43
expression of the	42
the recombinant ibvs	42
been shown to	42
three times with	42
the antiviral activity	42
for the treatment	42
was carried out	41
at h post	41
the in vitro	41
the plasma membrane	41
in the absence	41
the vero e	41
after h of	41
a variety of	40
of vero e	40
used as a	40
showed that the	40
the effects of	40
in the present	40
on vero cells	39
the use of	39
were determined by	39
by plaque assay	39
the infectivity of	39
of p mapk	39
vero e and	38
vitro and in	38
ck ch ldl	38
and in vivo	38
a novel coronavirus	38
characterization of the	38
at an moi	38
of viral rna	38
cell lines were	37
were fixed with	37
cells were seeded	36
green monkey kidney	36
the role of	36
the inhibition of	36
coronavirus infectious bronchitis	36
cells in the	36
viral rna was	36
vero cell line	36
washed three times	35
icpc a and	35
and vero cells	35
incubated for h	35
a and b	35
added to the	35
and characterization of	35
was observed in	35
washed twice with	35
the results of	34
intracellular extracts of	34
results showed that	34
the addition of	34
sequence of the	34
at hpi and	34
mouse hepatitis virus	34
into vero cells	34
and icpc a	34
vero and ipec	34
the intracellular extracts	33
were observed in	33
that of the	33
characterization of a	33
were used to	33
with or without	33
cells transfected with	32
the severe acute	32
used in the	32
to evaluate the	32
infected with pedv	32
sabin poliovirus type	32
of infected cells	31
at c for	31
as previously described	31
simplex virus type	31
the detection of	31
transmissible gastroenteritis virus	31
fusion activity of	31
is required for	31
were washed twice	31
of cell death	30
was performed using	30
at h p	30
in addition to	30
well plates were	30
the viral genome	30
region of the	30
and in the	30
cells were then	30
observed in the	29
cell fusion activity	29
multiplicity of infection	29
antiviral activity against	29
washed with pbs	29
has been shown	29
was detected in	29
results suggest that	29
the fact that	29
vero cells in	29
were washed with	29
as compared to	29
the mutant virus	29
with severe acute	28
gds and gds	28
total rna was	28
and gds strains	28
in cultured cells	28
the spike protein	28
prrsv and pedv	28
was removed and	28
at the same	28
a total of	28
was extracted from	28
the s gene	28
activity of the	28
pedv s protein	28
was used for	28
mutations in the	28
with fetal bovine	28
were incubated for	27
vero cells infected	27
a and icpc	27
middle east respiratory	27
were added to	27
with pbs and	27
a moi of	27
supernatants were collected	27
the united states	27
east respiratory syndrome	27
for h and	27
e cells were	27
of zikv infection	26
were incubated at	26
west nile virus	26
were collected at	26
vero cells with	26
than that of	26
of the two	26
used for the	26
supplemented with fetal	26
were detected in	26
in the united	26
the percentage of	26
vero e cell	26
cells were grown	25
different concentrations of	25
to determine whether	25
identification of a	25
of m f	25
of the coronavirus	25
these cell lines	25
was analyzed by	25
in vitro prophylactic	25
the amount of	25
and infected with	25
the activity of	25
results indicated that	25
viral rna synthesis	25
the other hand	25
g for min	25
on the other	25
at a concentration	25
the level of	25
were cultured in	24
of action of	24
infectious cdna clone	24
vero cell monolayers	24
used in this	24
of sars coronavirus	24
increasing concentrations of	24
well as the	24
there was no	24
western blot analysis	24
end of the	24
h of incubation	24
cdna clone of	24
in cells infected	24
consistent with the	24
determined by plaque	24
the case of	24
detected in the	24
to vero cells	24
cells were pre	24
indicated that the	24
of the cells	24
to those of	23
different cell lines	23
of peptide h	23
expression levels of	23
liu et al	23
associated with the	23
suggesting that the	23
the infected cells	23
protein of sars	23
to investigate the	23
three independent experiments	23
infection in vero	23
member of the	23
those of the	23
diarrhea virus in	23
in contrast to	23
a number of	23
shown to be	23
infected with sars	23
the end of	23
to each well	23
for the production	23
the gds and	23
inoculum was removed	23
length cdna clone	23
coronavirus spike protein	23
the viral rna	23
recombinant ibvs and	22
porcine reproductive and	22
were used for	22
and incubated for	22
of zika virus	22
involved in the	22
the virus was	22
as a negative	22
conjugated goat anti	22
j cells were	22
compared with the	22
of the genome	22
infection in vitro	22
at a moi	22
s gene mutants	22
development of a	22
and respiratory syndrome	22
as described in	22
reproductive and respiratory	22
were transfected with	22
vero cells at	22
vero cells was	22
h post infection	22
were fixed and	22
by western blot	22
the levels of	22
a multiplicity of	22
log copies ml	22
in the case	22
mechanism of action	22
the overexpression of	22
avian infectious bronchitis	22
a negative control	22
the mechanism of	22
times with pbs	22
mizutani et al	22
in our study	21
an inhibitor of	21
found to be	21
the induction of	21
culture supernatants were	21
be due to	21
pi k akt	21
viral titers in	21
of s protein	21
the plates were	21
in the same	21
characteristics of the	21
the host cell	21
or absence of	21
monolayers of vero	21
and then incubated	21
li et al	21
added to each	21
cells were inoculated	21
and western blotting	21
of the pedv	21
coronavirus associated with	21
s s cleavage	21
was detected by	21
antiviral effect of	21
cell monolayers were	21
and stained with	21
the parental virus	21
the s s	21
at h after	21
the virus titer	21
effect on vero	21
to that of	21
thermo fisher scientific	21
were used as	20
present in the	20
cov s protein	20
important role in	20
the beaudette strain	20
pcr and western	20
removed and the	20
twice with pbs	20
at the plasma	20
in response to	20
the rescued virus	20
and cells were	20
the supernatant was	20
in each group	20
propagated in vero	20
virus in the	20
induced cell death	20
derived from the	20
the culture medium	20
the n protein	20
the presence or	20
the growth kinetics	20
dependent rna polymerase	20
the lack of	20
cells were transfected	20
a concentration of	20
respiratory syndrome virus	20
in pbs for	20
s cleavage site	20
for the detection	20
determined using the	19
the synthetic viruses	19
observed in cells	19
were performed in	19
of the drug	19
zika virus infection	19
from vero cells	19
revealed that the	19
was determined using	19
transfected with the	19
have shown that	19
of the vero	19
were analyzed by	19
the sars coronavirus	19
were grown in	19
cells were cultured	19
at different time	19
in the culture	19
african swine fever	19
the study of	19
with wild type	19
the concentration of	19
in vero and	19
s and s	19
effect on the	19
vero cell lines	19
presence or absence	19
at a multiplicity	19
it has been	19
were seeded in	19
zhang et al	19
p and p	19
were stained with	19
an important role	19
reverse genetics system	19
a member of	19
and incubated with	19
the importance of	19
h at room	18
were washed three	18
the surface of	18
ibvs and beaudette	18
the generation of	18
cells were collected	18
ns a protein	18
were obtained from	18
to the cells	18
viral protein expression	18
human coronavirus e	18
different time points	18
assay was performed	18
spike protein of	18
the formation of	18
wild type and	18
activation of the	18
virus titers were	18
treatment of sars	18
the pedv s	18
the authors declare	18
can be used	18
infection of vero	18
cell lines and	18
but not in	18
balb c mice	18
has been reported	18
of the most	18
extracts of ln	18
two cell lines	18
ren et al	18
demonstrated that the	18
of blfp crude	18
for min and	18
of the mutant	18
the inoculum was	18
respiratory syncytial virus	18
virus titers in	18
that pedv entry	18
in the ipec	18
an in vitro	18
sequences of the	18
with vero cells	18
genome sequence of	18
fixed with paraformaldehyde	18
corresponding to the	17
found that the	17
supernatants were harvested	17
inhibitory effect of	17
e cells exposed	17
approved by the	17
cells were maintained	17
used as the	17
in terms of	17
monkey kidney cells	17
is thought to	17
effects of the	17
swine fever virus	17
then incubated with	17
in the viral	17
the emergence of	17
together with the	17
all of the	17
c and d	17
pcd and vero	17
isolation and characterization	17
u ml penicillin	17
and the cells	17
s protein of	17
wang et al	17
cell line was	17
was performed as	17
cells exposed to	17
patients with severe	17
of the protein	17
at rt for	17
were harvested at	17
the invasion rates	17
in the spike	17
chen et al	17
the icpc a	17
these results suggest	17
that can be	17
performed as described	17
human immunodeficiency virus	17
replication of sars	17
of chikungunya virus	17
kindly provided by	17
was performed to	17
were carried out	17
the coronavirus infectious	17
cleavage of the	17
activated protein kinase	17
the results showed	16
sam binding site	16
titers in the	16
the samples were	16
was amplified by	16
of viral infection	16
for h before	16
replication of the	16
lower than that	16
to the cell	16
were maintained in	16
inhibition of viral	16
and stored at	16
incubated at c	16
medium was removed	16
in the treatment	16
the ability of	16
a new coronavirus	16
of the antiviral	16
the helicase protein	16
in patients with	16
cell lines are	16
of herpes simplex	16
inoculated with the	16
the efficacy of	16
was evaluated by	16
was confirmed by	16
of the spike	16
vero cells by	16
was added at	16
the activation of	16
has also been	16
in the pedv	16
infectivity of icpc	16
a v mutation	16
suggest that the	16
influenza a virus	16
type i interferon	16
no significant difference	16
to assess the	16
and huh cells	16
vero cell culture	16
propagation of the	16
and cultured in	16
in combination with	16
as a result	16
porcine epidemic diarrhoea	16
the a protein	16
with pedv at	16
significantly higher than	16
virus infection in	16
for the development	16
pbs for min	16
is important to	16
were purchased from	16
cell lines for	16
cells and ipec	16
by using the	16
was isolated from	16
to test the	16
by western blotting	16
the porcine epidemic	16
did not show	16
pedv in vero	16
vesicular stomatitis virus	16
it is important	16
of dengue virus	16
the genome of	16
early stages of	16
in the future	16
with pedv strains	16
e and ma	16
have been used	15
in the supernatant	15
related to the	15
the virus in	15
min at room	15
e cells and	15
hpi and hpi	15
production of viral	15
the sam binding	15
virus replication in	15
were detected by	15
of the recombinant	15
on the cell	15
these results indicated	15
in sars patients	15
that m f	15
of the in	15
role in the	15
mutation in the	15
inhibition of the	15
incubated with pedv	15
prior to infection	15
vero and a	15
of the parental	15
c in a	15
the s domain	15
and a cells	15
shown in figure	15
was performed with	15
suggested that the	15
and vero e	15
egfr pi k	15
at the indicated	15
the expression levels	15
dengue virus type	15
to the parental	15
then infected with	15
the viability of	15
results suggested that	15
the ns a	15
the supernatants were	15
as early as	15
with different concentrations	15
was used in	15
c for min	15
in a co	15
the viral titers	15
obtained from the	15
is known to	15
were determined using	15
plates were infected	15
in vitro antiviral	15
surjit et al	15
and the virus	15
between the two	15
in accordance with	15
virus infection and	15
similar to the	15
significant difference between	15
of the porcine	15
differences in the	15
ifitm and ifitm	15
in cells expressing	15
the antiviral effect	15
virus entry and	15
showed that pedv	15
ml at hpi	15
and syncytia formation	15
study of the	15
the same time	15
are required for	15
of pedv in	15
changes in the	15
in the cell	15
no effect on	15
of the rescued	15
e cells in	15
was observed for	14
the impact of	14
these results demonstrate	14
were able to	14
the cell surface	14
were overlaid with	14
the ability to	14
to the sars	14
per well of	14
fixed and stained	14
in infected cells	14
for min to	14
experiments were performed	14
were resuspended in	14
antiviral activity in	14
infected with wild	14
be involved in	14
the inhibitory effect	14
the regulation of	14
cell culture and	14
h after the	14
vero who cells	14
tumor cell lines	14
genome copy number	14
such as the	14
in cells transfected	14
cells with the	14
the release of	14
of infectious bronchitis	14
of chikv ls	14
dmem supplemented with	14
intracellular viral rna	14
entry into vero	14
cov infection in	14
room temperature for	14
cell cultures and	14
used to infect	14
this work was	14
in the cytoplasm	14
porcine aminopeptidase n	14
treated with or	14
prophylactic and therapeutic	14
error bars represent	14
found in the	14
at days post	14
well plates at	14
is the first	14
the cytopathic effect	14
it is not	14
treated with the	14
the s subunit	14
vero cells treated	14
cotton rats were	14
in cell cultures	14
sequencing of the	14
respiratory syndrome in	14
that myricetin may	14
replication in vero	14
induced cytopathic effect	14
difference in the	14
extracted from the	14
cells per well	14
infected with hsv	14
was found to	14
s protein was	14
all cell lines	14
induction of apoptosis	14
in both cell	14
are shown in	14
membrane fusion and	14
stages of infection	14
of the study	14
the medium was	14
kidney cell line	14
cell infection by	14
the entry of	14
apoptosis in vero	14
expressed as the	14
lee et al	14
ml of the	14
of cells with	13
to induce apoptosis	13
understanding of the	13
was also observed	13
to generate the	13
which is a	13
japanese encephalitis virus	13
infected with chikv	13
was supported by	13
activity of s	13
be used for	13
associated with severe	13
of type i	13
in agreement with	13
is essential for	13
different cell types	13
samples were collected	13
a panel of	13
a result of	13
old a mice	13
similar to those	13
and chlamydia pecorum	13
epidemic diarrhoea virus	13
the infection of	13
cells and the	13
intestinal epithelial cells	13
is one of	13
mm nh cl	13
focus forming assay	13
could be detected	13
of the mean	13
against pedv in	13
and then the	13
both cell lines	13
when compared to	13
in the sam	13
fang et al	13
the infectious clone	13
rna was isolated	13
authors declare that	13
to this end	13
data suggest that	13
cytopathic effect on	13
vero cells for	13
hofmann and wyler	13
appeared to be	13
viral rna levels	13
serial dilutions of	13
lung epithelial cells	13
receptor for the	13
belongs to the	13
vero cells grown	13
invasion rates of	13
is able to	13
was no significant	13
the kinetics of	13
dilutions of the	13
by centrifugation at	13
the virus to	13
yount et al	13
virus control group	13
the pathogenesis of	13
by staining with	13
some of the	13
monkey kidney cell	13
pedv icpc a	13
for treatment of	13
were performed using	13
thought to be	13
mode of action	13
the detection limit	13
mice infected with	13
the possibility of	13
rna synthesis and	13
with the intracellular	13
and that the	13
the sequence of	13
cells and incubated	13
human coronavirus nl	13
at neutral ph	13
the membrane was	13
reduction in the	13
in different cell	13
activity against sars	13
pcr products were	13
and treated with	12
cell monolayers in	12
was able to	12
with the virus	12
nns rna viruses	12
is shown in	12
carried out by	12
at and h	12
is a member	12
of harringtonine was	12
emerging and re	12
is consistent with	12
rates of the	12
and h post	12
and pedv were	12
for h with	12
it is possible	12
carried out in	12
amino acid substitutions	12
inoculated with kpedv	12
the viral envelope	12
had no effect	12
may not be	12
the nucleocapsid protein	12
to the s	12
is necessary to	12
entry into cells	12
were collected from	12
genbank accession no	12
of a new	12
statistical significance was	12
significantly lower than	12
the difference in	12
open reading frame	12
the synthesis of	12
e cell line	12
effects on the	12
performed using the	12
genome of the	12
the two viruses	12
the nucleotide sequence	12
regulation of cell	12
the identification of	12
with ml of	12
overexpression of the	12
of this study	12
cells as described	12
the empty vector	12
was incubated with	12
dephosphorylation of stat	12
in vero cell	12
differences between the	12
have been reported	12
from kefir grains	12
were similar to	12
with the recombinant	12
cell death during	12
were compared to	12
binding and entry	12
growth kinetics of	12
the ratio of	12
compared to those	12
culture supernatant was	12
the cell lines	12
be able to	12
m f and	12
replication in vitro	12
were maintained at	12
well tissue culture	12
replication in cell	12
located in the	12
the plate was	12
belong to the	12
higher than that	12
proteolytic cleavage of	12
and in vitro	12
each well and	12
in the number	12
difference between the	12
of incubation at	12
d d d	12
lai et al	12
these results suggested	12
exposed to a	12
that the virus	12
cell lines to	12
the time of	12
in sars coronavirus	12
induce apoptosis in	12
lead to the	12
bovine serum albumin	12
of novel coronavirus	12
rt for min	12
viral replication in	12
were pretreated with	12
cells were pretreated	12
on the surface	12
to be a	12
in the cells	12
indicate that the	12
in pedv entry	12
of murine coronavirus	12
domain of the	12
plates were incubated	12
cells were harvested	12
effect of the	12
be used to	12
against zikv infection	12
able to induce	12
it will be	12
based on a	12
most of the	12
transmissible gastroenteritis coronavirus	12
open reading frames	12
infectivity of the	12
of viral proteins	12
were expressed as	12
used at a	12
each group were	12
the spike glycoprotein	12
cell lysates were	12
cell death in	12
viral rna in	12
that have been	12
in which the	12
pedv strains at	12
of the gds	12
cell lines that	11
days post infection	11
at least three	11
to study the	11
lactic acid bacteria	11
virus gd protein	11
activity of ribavirin	11
a receptor for	11
sars coronavirus infection	11
aminopeptidase n is	11
conformational changes in	11
titer was determined	11
depending on the	11
the vero and	11
vero cells are	11
at the time	11
of the ibv	11
for disease control	11
the most effective	11
antiviral activities of	11
h at c	11
min at rt	11
more sensitive to	11
type i and	11
the duplex rt	11
the gds strain	11
hou et al	11
of the e	11
was obtained from	11
to a fixed	11
there is an	11
k akt pathway	11
parts of the	11
we did not	11
and bile acids	11
and virus titers	11
titers were determined	11
cells did not	11
supplementary table s	11
in vitro transcription	11
targeting the s	11
than vero cells	11
amplified by rt	11
avian coronavirus infectious	11
propagation in vero	11
presence of trypsin	11
the design of	11
the mock group	11
care and use	11
concentration of sars	11
invasion efficiency of	11
a vero cell	11
comparison of the	11
with the mutant	11
sequence analysis of	11
vero cells as	11
resulted in a	11
at a density	11
treated with different	11
of virus infection	11
the complete genome	11
the invasion efficiency	11
of african green	11
the results were	11
induced by sars	11
considered statistically significant	11
to investigate whether	11
the m protein	11
in a dose	11
when cells were	11
also observed in	11
of viral vaccines	11
plaque reduction assay	11
the p mapk	11
infection and the	11
our results suggest	11
data were analyzed	11
effects of chloroquine	11
collected at hpi	11
analysis was performed	11
s protein is	11
world health organization	11
results of the	11
pedv n protein	11
more than of	11
protein of pedv	11
a density of	11
patients recovered from	11
viral replication and	11
green fluorescent protein	11
cells were observed	11
in l of	11
of the intracellular	11
h after infection	11
a fixed concentration	11
the n gene	11
there were no	11
in our laboratory	11
flavivirus e protein	11
minimum essential medium	11
fecal viral shedding	11
cells were stained	11
high level of	11
at the end	11
the s ntd	11
g a and	11
the g c	11
vero cell lineage	11
upstream of the	11
the mechanisms of	11
work was supported	11
against herpes simplex	11
cells were lysed	11
the feasibility of	11
with chlamydia abortus	11
was measured using	11
to of the	11
s protein and	11
were prepared by	11
in the virus	11
for use in	11
a set of	11
fixed concentration of	11
members of the	11
are shown as	11
no and peroxynitrite	11
the phosphorylation of	11
identity of the	11
and subjected to	11
washing with pbs	11
high levels of	11
with pfu of	11
isolation of sars	11
assays were performed	11
treated with trypsin	11
epidemic diarrhea in	11
the consensus sequence	11
of the murine	11
is the most	11
vero cells is	11
novel coronavirus associated	11
and maintained in	11
than those of	11
from the first	11
of the helicase	11
the ministry of	11
presence of increasing	11
be attributed to	11
experimental infection of	11
various concentrations of	11
has not been	11
was performed in	11
using graphpad prism	11
the drug was	11
sun et al	11
were then incubated	11
moi of pfu	11
and analyzed by	11
the murine coronavirus	11
the cell monolayer	11
mutation of the	11
the s and	11
tcid ml of	11
uncleaved s protein	11
associated with a	11
pcr was performed	11
none of the	11
of dynamin ii	11
of junin virus	11
was measured by	11
yellow fever virus	11
the culture supernatants	11
with crystal violet	11
with respect to	11
the indicated times	11
similar to that	11
fusion of the	11
the growth properties	11
hpi for qrt	11
described in the	11
in the footpad	10
mouse monoclonal antibody	10
previous studies have	10
confluent vero cells	10
responsible for the	10
and found that	10
was performed by	10
dengue virus infection	10
were then washed	10
ic value of	10
demonstrate that the	10
with the corresponding	10
and r l	10
prrsv or pedv	10
for the first	10
s protein on	10
and added to	10
vitro prophylactic effects	10
a plaque assay	10
determination of the	10
may also be	10
with acute respiratory	10
centers for disease	10
were also observed	10
cells were overlaid	10
and activation of	10
h prior to	10
virus from the	10
and expressed as	10
infection of the	10
nucleotide sequence of	10
antiviral efficacy of	10
containing the empty	10
comparable to the	10
the experiments were	10
to interfere with	10
into host cells	10
have been shown	10
were harvested and	10
were subjected to	10
caused by a	10
but not the	10
we determined that	10
cells grown in	10
and p mapk	10
virus is a	10
a concentration ranging	10
peptide h on	10
infection and replication	10
is possible that	10
rna was detected	10
the cell viability	10
virus growth medium	10
the amino acid	10
p mapk signaling	10
to analyze the	10
some of these	10
sialic acid binding	10
the small intestine	10
cpe was observed	10
type culture collection	10
equal volume of	10
significantly reduced the	10
the cell culture	10
the novel coronavirus	10
a functional receptor	10
were analyzed using	10
and transferred to	10
viral titer of	10
proteins in the	10
by using a	10
were removed by	10
cells were detected	10
vero cells have	10
entry at the	10
the replication cycle	10
transferred to a	10
deletion in the	10
of ammonium chloride	10
fold serial dilutions	10
of each compound	10
of stat in	10
of the p	10
single amino acid	10
cell culture supernatants	10
expression of s	10
activation of p	10
protein is a	10
the binding of	10
concentration ranging from	10
titer of the	10
cells at the	10
addition of trypsin	10
viral entry and	10
of transmissible gastroenteritis	10
to be the	10
a decrease in	10
signaling pathways are	10
f and c	10
of viral protein	10
with uncleaved s	10
in supplementary file	10
was quantified by	10
rna extracted from	10
american type culture	10
a review of	10
increase in the	10
were centrifuged at	10
and hpi for	10
used to treat	10
that they have	10
the footpad with	10
the early stages	10
proteolytic processing of	10
with ck ch	10
of the synthetic	10
was extracted using	10
after incubation for	10
carrying mutations in	10
inhibition of virus	10
morbidity and mortality	10
bile and bile	10
phosphorylation of p	10
a combination of	10
rna in the	10
presence of ribavirin	10
using vero cells	10
added to cells	10
apoptosis induced by	10
followed by a	10
pseudovirions with uncleaved	10
a and rmv	10
after washing with	10
derived from a	10
that the vero	10
of prrsv and	10
vero cells on	10
an equal volume	10
patients with covid	10
expression of viral	10
were euthanized at	10
cells in vitro	10
was calculated as	10
studies have shown	10
coronavirus infection in	10
for virus isolation	10
were monitored daily	10
were mixed with	10
feline infectious peritonitis	10
plasma membrane and	10
g c mutation	10
properties of the	10
of influenza a	10
of the human	10
used to detect	10
in the field	10
binding to the	10
may be due	10
weight loss and	10
of the full	10
known to be	10
s gene of	10
cells in a	10
and permeabilized with	10
of the first	10
supplemented with fbs	10
and the supernatant	10
to the control	10
temperature for h	10
in the two	10
important for the	10
and the s	10
determined by the	10
lin et al	10
virus to the	10
protein expression in	10
infectious virus was	10
determined by a	10
suggesting that myricetin	10
replication in the	10
infection by pedv	10
each of the	10
the potential to	10
in blocking buffer	10
amount of virus	10
amino acid sequences	10
reported to be	10
all of them	10
these data suggest	10
of increasing concentrations	10
of ebola virus	10
the parental pedvpt	10
tissue culture plates	10
than that in	10
an increase in	10
because of the	10
confirmed that the	10
and used to	10
of the viruses	10
there are no	10
we compared the	10
shown in table	10
such as sars	10
hepatitis b virus	10
in hong kong	10
of human coronavirus	10
mapk signaling pathway	10
incubated for min	10
cell viability was	10
with the s	10
infected cells was	10
in t cells	10
mediated by the	10
cells expressing s	10
there is no	10
cell death and	10
no significant differences	10
the s region	9
to confirm the	9
the virus life	9
infection of pigs	9
huang et al	9
enter cells through	9
treatment of zikv	9
porcine nidovirus replication	9
diarrhea in cell	9
virus infection of	9
virus inoculum was	9
inhibitory effect on	9
on the s	9
terminal domain of	9
of infectious virus	9
carried out using	9
ibv beaudette strain	9
as a control	9
part of the	9
gds strains were	9
h at rt	9
linked immunosorbent assay	9
in the family	9
treatment of vero	9
with a new	9
that there are	9
airway epithelial cells	9
the viral spike	9
and identification of	9
of the drugs	9
from patients recovered	9
both cell types	9
listed in table	9
of no and	9
be associated with	9
with the host	9
h before infection	9
with vero e	9
extract against pedv	9
an infectious cdna	9
days after challenge	9
growth properties of	9
were used at	9
i and type	9
data indicate that	9
were confirmed by	9
of mouse hepatitis	9
pigs in the	9
identification of the	9
length cdna clones	9
in vivo and	9
than of the	9
been reported to	9
cells containing the	9
of the genus	9
different stages of	9
of infected vero	9
elevated levels of	9
body weight loss	9
of antiviral drugs	9
there is a	9
isolation of the	9
by the addition	9
is a major	9
at the s	9
we tested the	9
is associated with	9
and sg mrna	9
virus in vitro	9
spike protein and	9
for both viruses	9
bars represent standard	9
e protein mab	9
piglets in the	9
for membrane fusion	9
in viral rna	9
crude extract against	9
of apoptosis by	9
for h in	9
protein has been	9
the virus titers	9
with chikv ls	9
of neutralizing antibodies	9
syndrome coronavirus spike	9
at the post	9
in vitro studies	9
by replacing the	9
the results indicated	9
of a sars	9
identified in the	9
e and f	9
of the antisense	9
trypsin for h	9
induction of cell	9
no conflict of	9
that vero cells	9
types of cells	9
concentrations of the	9
and it is	9
analyzed by western	9
the reduction of	9
described above and	9
infectious cdna clones	9
induced by the	9
lymphocytic choriomeningitis virus	9
also been reported	9
were visualized by	9
information about the	9
r l mutant	9
t cells expressing	9
results demonstrated that	9
was shown to	9
of the infected	9
plaque assay and	9
without blfp crude	9
western blotting assay	9
presence of the	9
human lung cells	9
pedv and mrv	9
study demonstrated that	9
vitro antiviral activity	9
on the virus	9
interaction with the	9
the majority of	9
determined that the	9
resulted in the	9
of the infectious	9
was found that	9
other cell lines	9
human airway epithelial	9
was allowed to	9
incubated with the	9
cell line is	9
was propagated in	9
amino acid substitution	9
incubated at room	9
appears to be	9
to inhibit the	9
a titer of	9
cell fusion and	9
the interaction of	9
virus life cycle	9
washing three times	9
the process of	9
is involved in	9
absence of trypsin	9
the function of	9
the primary antibody	9
may interact with	9
pfu of rzikv	9
be explained by	9
al kassaa et	9
and then infected	9
cells were co	9
but did not	9
the virus of	9
with an anti	9
could be a	9
recovery of infectious	9
complementary to the	9
were collected and	9
the focus forming	9
with fbs and	9
vero cells but	9
test was used	9
and all of	9
at d pi	9
against dengue virus	9
time points post	9
of sabin poliovirus	9
the onset of	9
to enter cells	9
did not induce	9
as an antiviral	9
infected with pfu	9
genomic rna and	9
strains at h	9
any of the	9
were fixed at	9
with an moi	9
infected with a	9
of growth factors	9
infected cells by	9
peptide h and	9
detection limit of	9
it was found	9
p p p	9
cells pretreated with	9
analysis showed that	9
effect of ribavirin	9
the isolation of	9
respiratory distress syndrome	9
is not essential	9
protein mab g	9
novel coronavirus in	9
of pedv icpc	9
kassaa et al	9
relative to the	9
to infect vero	9
considered to be	9
disease control and	9
syncytium formation was	9
a and ns	9
induces apoptosis in	9
in a cell	9
the specificity of	9
ns a and	9
identical to the	9
by flow cytometry	9
tryptose phosphate broth	9
of virus to	9
diarrhea virus strains	9
observed in vero	9
or medium alone	9
and type iii	9
the negative control	9
at this time	9
in vitro model	9
acid binding activity	9
virus of porcine	9
three cell lines	9
insights into the	9
after washing three	9
the most potent	9
indicating that the	9
mers pseudovirions with	9
by a novel	9
the capacity of	9
viral titers were	9
to replicate in	9
and pathogenesis of	9
efficiency of pedv	9
viral spike protein	9
e cells are	9
national institute of	9
site of the	9
is a potent	9
clarified by centrifugation	9
of the host	9
of cell lines	9
susceptible to infection	9
of viral replication	9
titer in the	9
virus produced in	9
important roles in	9
pedv entry into	9
was similar to	9
in both vero	9
h and then	9
insight into the	9
the data presented	9
of trypsin in	9
the tissue culture	9
are expressed as	9
did not affect	8
the respiratory tract	8
is responsible for	8
and use committee	8
specificity of the	8
genome sequences of	8
abortus and chlamydia	8
variants of porcine	8
institutional animal care	8
stage of zikv	8
passages in cell	8
as a potential	8
to infection with	8
were obtained by	8
in suckling piglets	8
the control of	8
swollen head syndrome	8
simmons et al	8
infected with junv	8
supernatants were determined	8
viral life cycle	8
virus titer in	8
added to vero	8
were performed to	8
using the pan	8
plates were washed	8
regulation of the	8
in the ipedvpt	8
the result of	8
is important for	8
infected with chlamydia	8
with diarrhea in	8
the cell monolayers	8
for in vitro	8
infectious peritonitis virus	8
with sterile pbs	8
virus replication by	8
for vaccine development	8
in the study	8
and immunogenicity of	8
functional receptor for	8
and h after	8
antiviral drug candidates	8
cell lines tested	8
the cells and	8
isolated from a	8
not show any	8
of the african	8
the international committee	8
and the resulting	8
urgent need to	8
final concentration of	8
did not significantly	8
to reduce the	8
the cell membrane	8
addition to the	8
to examine the	8
taxonomy of viruses	8
day after infection	8
reduction in viral	8
infected with ribv	8
the combination of	8
of the new	8
of the a	8
the world health	8
pedv infection was	8
was reported to	8
and ns b	8
porcine nidovirus infection	8
egfp reporter gene	8
could be used	8
removed by washing	8
entry and syncytia	8
various types of	8
and cloned into	8
results demonstrate that	8
genome landscape of	8
the titer of	8
e and huh	8
diluted in pbs	8
data are shown	8
not affect the	8
in the first	8
glycoprotein of murine	8
in a similar	8
mediated inhibition of	8
of different cell	8
associated with diarrhea	8
influenza a viruses	8
appear to be	8
are able to	8
is a functional	8
spike glycoprotein of	8
with paraformaldehyde in	8
from three independent	8
line of african	8
seven recombinant ibvs	8
viability of vero	8
monoclonal antibody against	8
shown to induce	8
we investigated the	8
was not observed	8
h after transfection	8
blood samples were	8
likely due to	8
acute respiratory distress	8
binding site in	8
strains for h	8
and evaluation of	8
is dependent on	8
international committee on	8
of chlamydial persistence	8
type and mutant	8
of intracellular viral	8
a high level	8
of hepatitis c	8
percentage of infected	8
pedv in the	8
cloned into the	8
gene expression of	8
graphpad prism software	8
nucleocapsid protein of	8
cells for h	8
in ml of	8
viral rna level	8
the establishment of	8
higher than those	8
infected cells and	8
minimal essential medium	8
with lm snap	8
apoptotic cell death	8
was found in	8
its antiviral activity	8
paraformaldehyde in pbs	8
was replaced with	8
of the vaccine	8
the infectious virus	8
using the reed	8
was shown that	8
oka et al	8
isolation of a	8
diarrhea virus infection	8
syndrome in hong	8
acquisition of the	8
cells and and	8
cell monolayer was	8
rather than the	8
to the virus	8
infectious bronchitis coronavirus	8
been used to	8
on the cellular	8
for min with	8
of the huh	8
fold dilutions of	8
is an important	8
of toremifene citrate	8
there have been	8
absence of growth	8
e cells infected	8
coronavirus a protein	8
protein of severe	8
and the cell	8
well plate format	8
wt and gfp	8
declare that they	8
pfu per cell	8
for vaccine production	8
transcripts containing the	8
the numbers of	8
with the same	8
of the three	8
was assessed by	8
detected in cells	8
plaques were counted	8
with two replicates	8
of mg ml	8
l mutant viruses	8
drug was added	8
the e protein	8
the recovery of	8
pretreated with the	8
shadrick et al	8
the occurrence of	8
n is a	8
the membranes were	8
to compare the	8
the quantification of	8
with the indicated	8
total number of	8
of this drug	8
ic values of	8
genes in vero	8
structural and functional	8
of virus yield	8
treatment of cells	8
generation of a	8
by incubation with	8
mechanism of pedv	8
yuan et al	8
control and prevention	8
in pi medium	8
respiratory disease in	8
log tcid ml	8
is independent of	8
was determined relative	8
the sars vaccine	8
activities of the	8
this study were	8
for hours at	8
not essential for	8
they have no	8
studies showed that	8
molecular biology of	8
genomic and subgenomic	8
of infectious diseases	8
percentage of ebov	8
infected with an	8
of the replication	8
the efficiency of	8
and the viral	8
monolayers were washed	8
were challenged with	8
to the viral	8
like cleavage site	8
or in combination	8
on the infection	8
the first time	8
infected and mock	8
an african green	8
tcid ml and	8
the entire genome	8
extracts of ypk	8
was treated with	8
lethal to the	8
the n transcripts	8
gene expression in	8
steps of the	8
infection in the	8
that peptide h	8
hantavirus replication in	8
virus entry at	8
then incubated at	8
the family coronaviridae	8
assay showed that	8
of an infectious	8
concentrations of drugs	8
strains in the	8
to further confirm	8
harringtonine was added	8
blot analysis of	8
cell spread of	8
a previous study	8
of the current	8
after h incubation	8
of s gene	8
in the early	8
were considered statistically	8
induced apoptosis in	8
antibody to the	8
described in this	8
expression in vero	8
fink et al	8
animal care and	8
mapped to the	8
during the first	8
viral rna and	8
was significantly lower	8
the course of	8
on the expression	8
inhibits the replication	8
park et al	8
new variants of	8
nm was measured	8
incubated with gds	8
caused by the	8
could not be	8
moi of for	8
for clinical use	8
the sensitivity of	8
of the rna	8
for minutes at	8
oh et al	8
of for h	8
with horseradish peroxidase	8
the cytoplasm of	8
the total number	8
a reverse genetics	8
the prevention of	8
of three independent	8
used to compare	8
it would be	8
associated with acute	8
by the severe	8
at various time	8
cell cycle progression	8
virus in porcine	8
with the parental	8
comparative analysis of	8
in lung epithelial	8
determined relative to	8
days of incubation	8
of the genomic	8
were approximately and	8
for h then	8
was observed when	8
infection of pedv	8
of gd protein	8
expression of a	8
required for the	8
we also assessed	8
the antiviral efficacy	8
to detect the	8
contrast to the	8
gene of the	8
amino acid sequence	8
the african green	8
and evolution of	8
we analyzed the	8
of the fusion	8
a monoclonal antibody	8
can be found	8
virus could be	8
the mutant rzikv	8
strand rna viruses	8
cells electroporated with	8
in this report	8
we demonstrated that	8
further characterization of	8
of viral particles	8
we developed a	8
various time points	8
have also been	8
for the inhibition	8
of sars patients	8
virus titer was	8
and and in	8
g g phase	8
were measured using	8
as the primary	8
able to inhibit	8
that the anti	8
novel coronavirus from	8
a lack of	8
of the five	8
it was shown	8
chlamydia abortus and	8
the virulent pedvpt	8
the a v	8
after removal of	8
is an urgent	8
in vitro transcribed	8
for the sars	8
p and pedvpt	8
of influenza virus	8
coronavirus nucleocapsid protein	8
have suggested that	8
or without myricetin	8
was observed at	8
in the lung	8
well plates and	8
be related to	8
apoptosis and necrosis	8
number of cells	8
viability of the	8
we hypothesized that	8
an urgent need	8
substitution in the	8
likely to be	8
in the lungs	8
based on these	8
for inhibition of	8
shown to have	8
we evaluated the	8
isolated from kefir	8
myricetin may interact	8
performed in triplicate	8
were found to	8
in these cell	8
s domain of	8
did not observe	8
viral stock was	8
a range of	8
activity against denv	8
remains to be	8
virus adsorption and	8
western blotting analysis	8
of the bacterial	8
levels of the	8
growth curves of	8
with porcine epidemic	8
of nitric oxide	8
vero cells to	8
of mers pseudovirions	8
by immunofluorescence assay	8
cells can be	8
were collected for	8
to pedv infection	8
are involved in	8
the two cell	8
to test this	8
spike protein is	8
is mediated by	8
viral and cellular	7
and progeny yield	7
can also be	7
isolated from the	7
the safety of	7
inhibitory activity of	7
later time points	7
of wild type	7
and human coronavirus	7
e cells at	7
c t values	7
rna replication and	7
to identify the	7
into the cytoplasm	7
of these s	7
vero jcrb cells	7
all animal experiments	7
as an internal	7
the receptor for	7
with proteinase k	7
su et al	7
the risk of	7
revealed that pedv	7
a co atmosphere	7
infected with zikv	7
infection with chlamydia	7
virus passage virus	7
gene was amplified	7
institute of biomedical	7
to characterize the	7
inhibition was determined	7
the most important	7
caused by sars	7
was added and	7
ammonium chloride was	7
in tissue culture	7
have been identified	7
from which the	7
with paraformaldehyde for	7
a dosage of	7
was performed on	7
in the coinfection	7
of pedv and	7
phosphorylated in virus	7
for this purpose	7
different types of	7
the peak viral	7
were mapped to	7
challenge with the	7
concentrations of chloroquine	7
cells stably expressing	7
with chlamydia and	7
be interesting to	7
prophylactic effects of	7
of the international	7
used to determine	7
the recently emerged	7
total rna extracted	7
mutant virus was	7
neutralization capacity of	7
then incubated for	7
infected mice were	7
with peptide h	7
concentrations of peptide	7
discontinuous transcription mechanism	7
contribute to the	7
data indicated that	7
were performed as	7
a wide range	7
well and incubated	7
was effective against	7
stocks were prepared	7
were blocked with	7
concentration of the	7
in southern china	7
in cell lines	7
day old pigs	7
virus stocks were	7
s protein by	7
of these cell	7
under an inverted	7
cell line vero	7
the existence of	7
and virus production	7
fig a and	7
fresh medium containing	7
there was a	7
with pneumonia in	7
a protein is	7
cells could be	7
pedv and chlamydia	7
correlation between the	7
alone or in	7
similar results were	7
were visualized using	7
of the icpc	7
production of infectious	7
to understand the	7
absence of virus	7
significant differences in	7
infected with either	7
as the mean	7
infection of human	7
infected cells treated	7
carried out with	7
titrated by plaque	7
s subunit of	7
early stage of	7
lu et al	7
the morphology of	7
committee on taxonomy	7
this study are	7
of ribavirin on	7
mediated endocytosis and	7
the transcription of	7
before virus infection	7
induced membrane fusion	7
rna copies ml	7
fixed with formaldehyde	7
cells was determined	7
in insect cells	7
k akt signaling	7
the mixture of	7
by the institutional	7
the identity of	7
cell line for	7
of cathepsin l	7
a a v	7
the zikv group	7
as severe acute	7
basis for the	7
polymerase chain reaction	7
and pi k	7
of ribavirin against	7
and vero cell	7
youn et al	7
in the spleen	7
the plaques were	7
of pedv entry	7
a single amino	7
least three independent	7
of these drugs	7
virus spike protein	7
the biology of	7
s region of	7
the heterogenous hvrs	7
the highest concentration	7
pfu of rmv	7
due to a	7
plates at a	7
more susceptible to	7
of the assay	7
site in the	7
in a cells	7
the percentages of	7
zhao et al	7
fresh vero cells	7
from a patient	7
a line of	7
the perinuclear region	7
to verify the	7
wide range of	7
in humans and	7
have no competing	7
etiological agent of	7
a cell line	7
was subjected to	7
of all the	7
effects against pedv	7
cell line and	7
p in vero	7
plos one doi	7
analysis of viral	7
of pfu cell	7
s ribosomal rna	7
were prepared in	7
be responsible for	7
ns a a	7
h before virus	7
virus stock was	7
extracellular viral rna	7
the early stage	7
death during infection	7
replication cycle of	7
on the plasma	7
temperature for min	7
of cyclophilin a	7
vitro prophylactic and	7
the mers pseudovirions	7
a role in	7
infected with each	7
cov a protein	7
was generated by	7
cells but not	7
the culture supernatant	7
infected with sads	7
the in vivo	7
with the control	7
numbers on the	7
coronavirus in patients	7
from sars patients	7
newcastle disease virus	7
we thank dr	7
as described elsewhere	7
into vero e	7
the roles of	7
removal of the	7
stages of the	7
processing of the	7
rna mini kit	7
to the results	7
value of the	7
phylogenetic analysis of	7
in the other	7
of vesicular stomatitis	7
tan et al	7
food and drug	7
a fluorescence microscope	7
its propagation in	7
a low ph	7
in each well	7
prior to the	7
vero cells from	7
entry into host	7
after the first	7
stained with anti	7
with the following	7
infected cells in	7
that the pedv	7
equal amounts of	7
into the host	7
fusion activities of	7
cov n protein	7
was purchased from	7
time course analysis	7
after days of	7
swine enteric alphacoronavirus	7
of clinical signs	7
cell metabolic activity	7
continuous cell lines	7
to elucidate the	7
passaged in vero	7
murine coronavirus spike	7
it should be	7
of cytopathic effect	7
in more than	7
may lead to	7
s protein to	7
to block the	7
and allowed to	7
pretreatment of cells	7
with a p	7
with virus gd	7
the second transfection	7
is characterized by	7
this may be	7
inhibition of sars	7
in cotton rats	7
myricetin may be	7
the virus and	7
to that end	7
were generated by	7
a protein of	7
inoculated with sads	7
does not inhibit	7
activity in vitro	7
borne encephalitis virus	7
yang et al	7
were significantly higher	7
of the ns	7
protein in the	7
and rpoa gene	7
decrease in the	7
cell fusion activities	7
of a single	7
t cells and	7
the virus inoculum	7
proteolytic activation of	7
the reason for	7
plates until confluence	7
were performed at	7
syndrome coronavirus in	7
the institutional animal	7
are needed to	7
of the l	7
inhibit the replication	7
into the cells	7
structural basis for	7
n protein was	7
these cells were	7
viruses such as	7
human cell lines	7
virus from p	7
bac cdna clone	7
western blotting at	7
of the growth	7
porcine enteric coronavirus	7
of chloroquine on	7
in the beaudette	7
with fresh medium	7
have already been	7
vt y was	7
is also shown	7
and isg genes	7
infection of sars	7
to the same	7
in severe acute	7
days two and	7
scale bars indicate	7
in the supernatants	7
ml of dmem	7
of coronavirus disease	7
we showed that	7
mns a was	7
like to thank	7
virus present in	7
with an equal	7
to vero e	7
min and then	7
for vero e	7
both vero and	7
titers in supernatants	7
determined to be	7
were seeded at	7
type and ribv	7
gds or gds	7
of innate immune	7
by interfering with	7
rna and sg	7
h incubation at	7
antiviral activity and	7
of the small	7
of n protein	7
value of this	7
and porcine epidemic	7
protein kinase b	7
at this position	7
of patients with	7
virus entry into	7
effective in inhibiting	7
that has been	7
hematopoietic cell lines	7
week old pigs	7
in more detail	7
a mixture of	7
reported to have	7
cell culture model	7
infection of cells	7
at days two	7
of the manuscript	7
gene copies ml	7
of the expression	7
novel human betacoronavirus	7
cleavage site of	7
virus into vero	7
interfere with the	7
of the gene	7
the wt sequence	7
were introduced into	7
female balb c	7
and determination of	7
of enveloped viruses	7
was normalized to	7
activities of these	7
was identified as	7
animal experiments were	7
to the wt	7
vero cells using	7
which recognizes residues	7
of the same	7
the coinfection group	7
measured using a	7
leading to the	7
was applied to	7
genetics system for	7
were fixed in	7
to vero cell	7
and replication of	7
mediated membrane fusion	7
into the cell	7
this suggests that	7
to days post	7
endosomes and lysosomes	7
fusion activity by	7
transcripts generated from	7
of the novel	7
confluent monolayers of	7
play an important	7
after the second	7
were normalized to	7
viral rna replication	7
efficacy of the	7
the virus is	7
and titration of	7
and viral rna	7
pedv infection in	7
of the other	7
to be effective	7
p mapk activation	7
effect on viral	7
and use of	7
were isolated from	7
cells were compared	7
closely related to	7
ml penicillin and	7
well as in	7
clinical management of	7
sample t test	7
e cell culture	7
and membrane fusion	7
cells were resuspended	7
c under co	7
late endosomes and	7
resulting in a	7
diarrhea virus strain	7
this study is	7
to our knowledge	7
cdna clones of	7
and apoptosis in	7
human and animal	7
genetic stability of	7
p mapk is	7
and n proteins	7
cyclophilin a is	7
one representative experiment	7
from patients with	7
well as to	7
has been used	7
of signaling pathways	7
the same virus	7
vero and huh	7
was significantly higher	7
of the family	7
in group were	7
which is consistent	7
between the pedv	7
similar to other	7
the first step	7
stability of the	7
s proteins of	7
chikv ls and	7
evaluation of the	7
were propagated in	7
ace and tmprss	7
separated by sds	7
of at least	7
the reduction in	7
involved in pedv	7
has been previously	7
explained by the	7
effect against pedv	7
medium was added	7
we also found	7
coronavirus spike proteins	7
could inhibit the	7
hoffmann et al	7
lines were infected	7
levels of viral	7
activator of transcription	7
when the cells	7
or in the	7
for the presence	7
of zikv rna	7
in escherichia coli	7
were removed and	7
of each virus	7
have been previously	7
levels of zikv	7
activity of ata	7
identification and characterization	7
within the helicase	7