quadgram

This is a table of type quadgram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

quadgram	frequency
severe acute respiratory syndrome	464
in the presence of	346
porcine reproductive and respiratory	294
reproductive and respiratory syndrome	291
acute respiratory syndrome coronavirus	284
and respiratory syndrome virus	257
of mouse hepatitis virus	225
cells were infected with	196
in the absence of	181
as shown in fig	180
of severe acute respiratory	148
human immunodeficiency virus type	123
in the case of	122
of porcine reproductive and	112
in cells infected with	107
it is possible that	103
has been shown to	102
porcine epidemic diarrhea virus	98
middle east respiratory syndrome	97
coronavirus infectious bronchitis virus	93
cells were transfected with	90
east respiratory syndrome coronavirus	82
this work was supported	81
the presence of the	80
of the s protein	79
work was supported by	76
have been shown to	75
at an moi of	75
in the present study	74
of the murine coronavirus	73
the severe acute respiratory	73
the central nervous system	71
the presence of a	68
washed three times with	68
these results suggest that	67
similar to that of	67
min at room temperature	67
coronavirus mouse hepatitis virus	65
an important role in	65
rna was extracted from	63
it has been shown	62
on the other hand	62
for min at room	61
of hepatitis c virus	61
at a multiplicity of	60
used in this study	59
was used as a	59
as well as the	59
cells were treated with	58
with severe acute respiratory	57
of the e protein	57
incubated for h at	56
avian infectious bronchitis virus	56
presence or absence of	56
of human immunodeficiency virus	55
these data suggest that	55
the cells were washed	55
sequence analysis of the	55
on the cell surface	54
under materials and methods	54
has been shown that	54
a multiplicity of infection	54
a member of the	54
to the cell surface	53
play a role in	53
in vitro and in	52
been shown to be	52
e and m proteins	52
the coronavirus infectious bronchitis	51
were washed three times	51
these results indicate that	51
nucleotide sequence of the	51
described under materials and	51
of the viral genome	50
the expression of the	50
for the presence of	50
was found to be	50
under the control of	49
in the context of	49
to that of the	48
mouse hepatitis virus strain	48
vitro and in vivo	47
as described under materials	47
the sequence of the	47
in contrast to the	47
the presence or absence	47
of herpes simplex virus	47
feline infectious peritonitis virus	46
for h at c	46
herpes simplex virus type	46
the role of the	45
cells were washed with	45
in the presence or	45
on the surface of	45
the end of the	44
end of the genome	44
was added to the	44
hepatitis virus strain a	44
and incubated for h	44
h at room temperature	44
in addition to the	44
of the genomic rna	43
at the cell surface	43
of a novel coronavirus	42
cd and cd t	42
amino acid sequence of	42
is consistent with the	42
it is likely that	42
are shown in fig	40
african swine fever virus	40
with fetal bovine serum	40
cells transfected with the	40
for h at room	40
the s protein of	40
by the addition of	39
in the regulation of	39
national institutes of health	38
of the s gene	38
cells infected with the	38
mouse mammary tumor virus	38
analyzed by western blot	38
play an important role	37
cells were incubated with	37
the human immunodeficiency virus	37
the molecular biology of	37
the cells were incubated	36
the innate immune response	36
the amino acid sequence	36
a role in the	36
of murine hepatitis virus	36
of porcine epidemic diarrhea	36
southern bean mosaic virus	36
of the n protein	35
amino acid sequences of	35
is not essential for	35
the ability of the	35
a final concentration of	35
in materials and methods	34
was detected in the	34
were washed with pbs	34
a wide range of	34
to determine whether the	34
porcine transmissible gastroenteritis virus	34
the size of the	34
the cells were then	34
is a member of	34
the sg start site	34
by agarose gel electrophoresis	34
identification of a novel	34
and cd t cells	34
supplemented with fetal bovine	34
these data indicate that	33
were analyzed by sds	33
h n pdm infection	33
in cells transfected with	33
in the replication of	33
total rna was extracted	33
of infectious bronchitis virus	33
the results showed that	33
on the basis of	33
the s glycoprotein gene	32
and the cells were	32
was supported by the	32
washed with pbs and	32
of the spike protein	32
described in materials and	32
were incubated for h	32
mouse hepatitis virus a	32
by western blot analysis	32
during the course of	32
the porcine reproductive and	32
incubated for min at	32
as described in materials	32
were found to be	31
murine coronavirus spike protein	31
the control of the	31
of three independent experiments	31
three times with pbs	31
we were able to	31
of the severe acute	31
the majority of the	31
de haan et al	30
we found that the	30
h n f l	30
was shown to be	30
in the cns of	30
may be due to	30
at the nonpermissive temperature	30
proteolytic cleavage of the	30
in the cytoplasm of	30
of the coronavirus infectious	30
was used as the	30
peste des petits ruminants	30
of the e glycoprotein	29
with respect to the	29
at room temperature for	29
as a result of	29
results indicate that the	29
by the presence of	29
of vesicular stomatitis virus	29
cells were washed twice	29
with a mixture of	29
of transmissible gastroenteritis coronavirus	29
studies have shown that	29
is possible that the	29
results suggest that the	29
as a template for	28
is not required for	28
associated with severe acute	28
i i i i	28
proteolytic processing of the	28
for h at rt	28
could be due to	28
in the s protein	28
identification and characterization of	28
were washed twice with	28
the a and b	28
of feline infectious peritonitis	28
for the synthesis of	28
at the end of	28
in the central nervous	28
the possibility that the	28
is shown in fig	27
as well as in	27
of transmissible gastroenteritis virus	27
at the plasma membrane	27
were washed once with	27
cells were fixed with	27
as described in the	27
the cytoplasmic tail of	27
reverse genetics system for	27
the murine coronavirus spike	27
e protein ion channel	27
it was found that	27
in order to determine	27
at c for h	27
coronavirus associated with severe	26
the specificity of the	26
to be involved in	26
kindly provided by dr	26
at a concentration of	26
cells were grown in	26
were detected in the	26
of the influenza virus	26
red clover necrotic mosaic	26
the crystal structure of	26
absence of e protein	26
american type culture collection	26
cells were seeded in	26
vero cells were infected	26
to the plasma membrane	26
the formation of the	26
plays a role in	26
alveolar type i cells	26
of influenza a virus	26
be due to the	26
is likely to be	26
in the formation of	26
this is the first	26
avian coronavirus infectious bronchitis	26
the cells were fixed	26
the jhm strain of	26
was performed using the	25
western blot analysis of	25
cells were labeled with	25
pandemic h n influenza	25
see materials and methods	25
at a moi of	25
was performed as described	25
the coronavirus mouse hepatitis	25
novel coronavirus associated with	25
cell lysates were prepared	25
dbt cells were infected	25
that the presence of	25
these results demonstrate that	25
that the e protein	25
of the n gene	25
we were unable to	25
is required for efficient	25
it has been suggested	25
the activation of the	25
cells were maintained in	25
determined by plaque assay	25
for the first time	24
cells were incubated for	24
to determine if the	24
respiratory syndrome coronavirus spike	24
plays an important role	24
amino acids of the	24
the structure of the	24
for the expression of	24
g to s phase	24
at the same time	24
this article can be	24
g for min at	24
article can be found	24
a novel coronavirus associated	24
by western blot with	24
cells were incubated at	24
was added to each	24
the pcr products were	24
were infected with the	24
de wilde et al	24
complete nucleotide sequence of	24
of southern bean mosaic	24
cells were then washed	23
an equal volume of	23
as a receptor for	23
hepatitis c virus infection	23
was similar to that	23
in the united states	23
the gene encoding the	23
the absence of e	23
it has been reported	23
at the time of	23
mice infected with a	23
is one of the	23
in the pathogenesis of	23
the deduced amino acid	23
at a density of	23
des petits ruminants virus	23
cd c cd b	23
it is conceivable that	23
clover necrotic mosaic virus	23
isolation and characterization of	23
hek t cells were	23
patients with severe acute	23
infected with wild type	23
of middle east respiratory	23
as a negative control	23
for the development of	22
and analyzed by western	22
the cavv cl pro	22
in balb c mice	22
for min at c	22
deduced amino acid sequence	22
cells were incubated in	22
ibv e and m	22
infection of the cns	22
in pbs for min	22
rule out the possibility	22
an integral membrane protein	22
similar to those of	22
has been suggested that	22
and characterization of a	22
of the nucleocapsid protein	22
a functional receptor for	22
the spike protein of	22
requests for reprints should	22
bacteriophage t rna polymerase	22
of the human coronavirus	22
of the human immunodeficiency	22
the synthesis of a	22
of the sars coronavirus	22
of the cleavage site	22
the case of the	21
the mechanism by which	21
is associated with the	21
compared to that of	21
van dinten et al	21
for reprints should be	21
complete genome sequence of	21
h n and h	21
to whom requests for	21
acid sequence of the	21
the hepatitis c virus	21
for the detection of	21
hepatitis c virus replication	21
specific cd t cells	21
the e protein is	21
for h and then	21
analyzed by electrophoresis on	21
in vitro transcription and	21
on ice for min	21
the national institutes of	21
mutational analysis of the	21
data indicate that the	21
mutants of mouse hepatitis	21
washed twice with pbs	21
incubated at c for	21
cells were washed three	21
the nucleotide sequence of	21
whom requests for reprints	21
in agreement with the	21
be involved in the	21
of cd t cells	21
when compared to the	21
of mice infected with	21
are consistent with the	21
little is known about	21
in patients with severe	21
venezuelan equine encephalitis virus	21
a large number of	21
of influenza a viruses	21
the e glycoprotein of	21
n and h n	21
that the expression of	21
terminal region of the	21
of the innate immune	21
respiratory syndrome coronavirus infection	21
of the porcine reproductive	20
in the number of	20
african green monkey kidney	20
the nucleocapsid protein of	20
murine coronavirus mouse hepatitis	20
dmem supplemented with fbs	20
of the central nervous	20
reprints should be addressed	20
is involved in the	20
at different time points	20
as compared to the	20
within the brains of	20
a and orf b	20
of the amino acid	20
hepatitis c virus rna	20
the viral life cycle	20
is required for the	20
the rest of the	20
protein was detected in	20
to the golgi complex	20
were transfected with the	20
was observed in the	20
labeled with s methionine	20
de groot et al	20
in any of the	20
cells were stained with	20
this is consistent with	20
at g for min	20
orf a and orf	20
have shown that the	20
of influenza c virus	20
was not detected in	20
it is also possible	20
with fetal calf serum	20
is known to be	20
molecular biology of coronaviruses	20
the importance of the	20
the synthesis of the	20
than that of the	20
in the brains of	20
has been reported for	20
this suggests that the	20
the a strain of	20
described in the legend	20
is also possible that	20
the p mapk pathway	20
data suggest that the	20
did not affect the	20
strain of mouse hepatitis	20
the human coronavirus e	20
products were analyzed by	20
levels of viral rna	20
in accordance with the	19
cells in the presence	19
it is known that	19
were infected with mhv	19
had no effect on	19
and then infected with	19
the ibv e protein	19
incubated for hr at	19
also been shown to	19
cells were washed once	19
was kindly provided by	19
has been reported that	19
crystal structure of the	19
it is not clear	19
cells were cultured in	19
the position of the	19
requests should be addressed	19
were added to the	19
in the production of	19
rna was isolated from	19
in the amount of	19
were found in the	19
were determined by plaque	19
was carried out using	19
l m t m	19
were performed as described	19
s and s pn	19
may play a role	19
the surface of the	19
rna of mouse hepatitis	19
infectious bronchitis virus e	19
and precipitated with ethanol	19
reprint requests should be	19
to be required for	19
the infectious bronchitis virus	19
the t rna polymerase	19
of the viral envelope	19
in a variety of	19
of the spike glycoprotein	19
performed as previously described	19
innate and adaptive immune	19
the s and s	19
we have shown that	19
the total number of	19
in the respiratory tract	19
on the presence of	19
one of the most	19
similar results were obtained	19
are involved in the	19
the results indicate that	19
the open reading frame	19
is known about the	19
balb cv tumor cells	19
syndrome coronavirus spike protein	19
of ibv n protein	18
supplementary data associated with	18
for min at rt	18
the fact that the	18
for hepatitis c virus	18
associated with this article	18
washed three times in	18
cells were lysed in	18
and analyzed by sds	18
loss of enteric tropism	18
amino acid substitutions in	18
has also been reported	18
multiplicity of infection of	18
the stability of the	18
this result suggests that	18
with wild type and	18
of the gene encoding	18
in the online version	18
it is important to	18
clarified by centrifugation at	18
followed by cycles of	18
was carried out in	18
cells were harvested and	18
been shown that the	18
is likely that the	18
u mg cells were	18
carried out as described	18
were cloned into the	18
the interaction between the	18
from cells infected with	18
could be detected in	18
it is unlikely that	18
pcr was performed using	18
is a functional receptor	18
h n pdm influenza	18
used as a template	18
and cloned into the	18
in each of the	18
m and e proteins	18
internal ribosome entry site	18
the presence of tunicamycin	18
the s protein is	18
cells were pretreated with	18
of african swine fever	18
of infected cells was	18
with this article can	18
a novel coronavirus in	18
is essential for the	18
data associated with this	18
relative to the sgrna	18
it is interesting to	18
the location of the	18
t rna polymerase promoter	18
in vero e cells	18
remains to be determined	18
the avian coronavirus infectious	18
barley stripe mosaic virus	18
in a beckman sw	17
n a l p	17
proteins b and ab	17
wild type and mutant	17
important role in the	17
in the expression of	17
in the s gene	17
may be involved in	17
in the infected cells	17
terminal domain of the	17
for the replication of	17
was observed in cells	17
revealed the presence of	17
in the synthesis of	17
the function of the	17
in dmem supplemented with	17
tomato bushy stunt virus	17
the large di rna	17
o u r n	17
cells were fixed and	17
in this study we	17
was performed using a	17
the sequences of the	17
the amino acid sequences	17
is based on the	17
at room temperature with	17
cells infected with wild	17
in the current study	17
in the s glycoprotein	17
and incubated for min	17
lower than that of	17
to confirm that the	17
vitro transcription and translation	17
l p r e	17
of porcine transmissible gastroenteritis	17
is responsible for the	17
is a determinant of	17
by grants from the	17
r o o f	17
interesting to note that	17
is mediated by the	17
protein of severe acute	17
to be important for	17
with h n pdm	17
a critical role in	17
did not result in	17
at a dilution of	17
p r o o	17
identification of a new	17
r n a l	17
characterization of a novel	17
for the generation of	17
of viral rna in	17
the causative agent of	17
from the national institutes	17
to a final concentration	17
western blot analysis using	17
a l p r	17
was removed and the	17
other members of the	17
in the generation of	17
presence of actinomycin d	17
of the rub genome	17
u r n a	17
the pandemic h n	17
the prrsv e protein	17
j o u r	17
by in vitro translation	17
to interact with the	17
the presence of actinomycin	17
for their ability to	16
van marle et al	16
for min on ice	16
t cells were co	16
in the development of	16
in mice infected with	16
the infectivity of the	16
in vitro translation of	16
c bl mice were	16
was carried out with	16
a small amount of	16
of hepatitis b virus	16
of the in vitro	16
was supported by grants	16
on a agarose gel	16
shown to interact with	16
were obtained from the	16
was found in the	16
the pcr product was	16
it should be noted	16
at rpm for min	16
performed as described previously	16
added to each well	16
functional receptor for the	16
and transferred to a	16
the region of the	16
the cells were harvested	16
end of the leader	16
in the induction of	16
at the permissive temperature	16
of the e gene	16
with that of the	16
is interesting to note	16
in a previous study	16
in the lungs of	16
and the presence of	16
it has been demonstrated	16
of murine coronavirus gene	16
the assembly of the	16
may be important for	16
of type i ifn	16
data are presented as	16
the middle east respiratory	16
protein is essential for	16
of the virus in	16
a single amino acid	16
which is consistent with	16
novel coronavirus in patients	16
were collected from the	16
the detection of the	16
of the genome of	16
of japanese encephalitis virus	16
coronavirus in patients with	16
in planar lipid bilayers	16
to investigate whether the	16
the top of the	16
the herpes simplex virus	16
these results indicated that	16
the loss of enteric	16
be explained by the	16
the length of the	16
subgenomic di rna transcription	16
from cells transfected with	16
of the infectious bronchitis	16
that the majority of	16
the s subunit of	16
of the he protein	16
were kindly provided by	16
enhanced green fluorescent protein	16
in the infected cell	16
in porcine reproductive and	16
these data demonstrate that	16
infectious bronchitis virus a	16
of the coronavirus mouse	16
times with pbs and	16
can be found in	16
amino acids in the	16
at c for min	16
a and b orfs	16
replication in cell culture	16
in the spike protein	16
the american type culture	16
cells were then incubated	16
pellets were resuspended in	16
results showed that the	16
and adaptive immune responses	16
cells as well as	15
the molecular basis of	15
as well as a	15
rpm for h at	15
numbers on the left	15
open reading frame of	15
for the production of	15
after h of incubation	15
has been reported to	15
have been identified in	15
terminal amino acids of	15
virus replication in the	15
pp a and pp	15
at least in part	15
would like to thank	15
the s protein was	15
postweaning multisystemic wasting syndrome	15
the cell lysates were	15
incubated on ice for	15
of the coronavirus mhv	15
to the presence of	15
it is clear that	15
subjected to immunoprecipitation with	15
our results suggest that	15
in the er and	15
supported by grants from	15
innate immune response to	15
the atpase activity of	15
coronavirus transmissible gastroenteritis virus	15
functional characterization of the	15
as compared to wild	15
committee on taxonomy of	15
end of the viral	15
identical to that of	15
in the lipid rafts	15
the sam binding site	15
were fixed with paraformaldehyde	15
infected or infected with	15
the s gene of	15
the e protein in	15
and functional characterization of	15
the expression levels of	15
can be used to	15
gene encoding the putative	15
from that of the	15
infected cells in the	15
with mouse hepatitis virus	15
a molecular weight of	15
was carried out as	15
of the sequence of	15
the complete nucleotide sequence	15
nucleotide and amino acid	15
were separated by sds	15
for the study of	15
a portion of the	15
is thought to be	15
to s phase transition	15
has been demonstrated that	15
of cd c cells	15
bronchitis virus e protein	15
for excellent technical assistance	15
of the infected cells	15
for h prior to	15
the inhibitory effect of	15
were infected with wt	15
of the peplomer gene	15
the structural proteins of	15
at and days post	15
in the form of	15
out the possibility that	15
by the method of	15
in tissue culture cells	15
protein has been shown	15
the n protein of	15
by the use of	15
essential for viral replication	15
pellet was resuspended in	15
in a mouse model	15
a strain of mhv	15
amplified by pcr using	15
of west nile virus	15
alveolar type ii cells	15
the s proteins of	15
the mhv e protein	15
removed and the cells	15
international committee on taxonomy	15
acid sequences of the	15
of subgenomic di rna	15
after h n pdm	15
of the s glycoprotein	15
of equine arteritis virus	15
not present in the	15
respiratory syndrome coronavirus in	15
cells infected with ribv	15
incubated at room temperature	14
virus titers were determined	14
on taxonomy of viruses	14
was determined by plaque	14
should be noted that	14
in a volume of	14
whom reprint requests should	14
ts mutant s protein	14
were observed in the	14
be found in the	14
in infected cells and	14
the development of a	14
then washed three times	14
a and pp ab	14
used as a negative	14
cells were inoculated with	14
followed by incubation with	14
on the left indicate	14
with the exception of	14
h n influenza virus	14
of mouse mammary tumor	14
permeabilizing activity of the	14
phosphorylation of ibv n	14
of the s subunit	14
of type i interferon	14
vero cells infected with	14
coronavirus avian infectious bronchitis	14
products were cloned into	14
was supported by a	14
room temperature for min	14
to be associated with	14
has also been shown	14
but not in the	14
plates were infected with	14
been reported to be	14
infection of the central	14
the presence of tm	14
extracted with phenol chloroform	14
a component of the	14
for subgenomic mrna synthesis	14
e protein in coronavirus	14
for min and then	14
to whom reprint requests	14
cells were fixed in	14
the largest rna virus	14
was obtained from the	14
pbs for min at	14
positive strand rna viruses	14
n protein was detected	14
the effect of the	14
these findings suggest that	14
an infectious cdna clone	14
respiratory syndrome virus infection	14
to the absence of	14
intracellular rna was extracted	14
the genomic rna of	14
results indicated that the	14
the entire genome of	14
of avian infectious bronchitis	14
the international committee on	14
by the fact that	14
the respiratory tract of	14
atpase activity of prrsv	14
our understanding of the	14
of the immune system	14
by plaque assay on	14
mutations in the s	14
tempting to speculate that	14
and analysis of the	14
the coding region of	14
at a final concentration	14
functional analysis of the	14
protein ion channel activity	14
these results suggested that	14
protein was detected by	14
we have previously shown	14
monolayers of vero cells	14
representative of three independent	14
in the absence or	14
were incubated in the	14
is in agreement with	14
in the legend to	14
of the polymerase gene	14
of viral rna synthesis	14
cells were washed in	14
deduced amino acid sequences	14
of amino acid residues	14
is supported by the	14
titers were determined by	14
and characterization of the	14
it was shown that	14
was extracted from the	14
were labeled with s	14
at least three independent	14
the bottom of the	14
and the number of	14
the m protein of	14
have been reported to	14
a beckman sw rotor	14
containing fetal bovine serum	14
the murine coronavirus mhv	14
de vries et al	14
compared to wild type	14
of a murine coronavirus	14
infected with mouse hepatitis	14
with t dna polymerase	14
supported in part by	14
was used to determine	14
fragmentation of the ga	14
from the american type	14
for western blot analysis	14
the generation of a	14
the cells were infected	14
by electrophoresis on a	14
terminal half of the	14
and the role of	14
and expression of the	14
between amino acids and	14
major histocompatibility complex class	14
hepatitis c virus core	14
medium was replaced with	13
c h hen background	13
were infected with a	13
were extracted with phenol	13
vivo and in vitro	13
members of the family	13
well as in the	13
hepatitis b surface antigen	13
the replication of rna	13
to further investigate the	13
internal entry of ribosomes	13
of red clover necrotic	13
genome and proteome of	13
lysates were subjected to	13
to western blot analysis	13
leader rna sequences on	13
was performed as previously	13
jhm strain of mouse	13
was determined by the	13
it was demonstrated that	13
ns and ns proteins	13
african horse sickness virus	13
this region of the	13
a deletion in the	13
of semliki forest virus	13
be due to a	13
cov s and s	13
of the parental jhm	13
of the host cell	13
comparable to that of	13
are representative of three	13
unique and conserved features	13
on the expression of	13
flanking sequences of the	13
the activity of the	13
released into the supernatant	13
of cd and cd	13
at and h p	13
of wild type and	13
was used to detect	13
and the expression of	13
as well as for	13
protein encoded by the	13
brain and spinal cord	13
detected in cells infected	13
features of genome and	13
respiratory syndrome coronavirus envelope	13
pathogenic porcine reproductive and	13
the course of the	13
through a sucrose cushion	13
clades a to d	13
probably due to the	13
the k and k	13
the relative amount of	13
not required for the	13
and subjected to immunoprecipitation	13
for each of the	13
the predicted amino acid	13
absence or presence of	13
the genome of the	13
to ensure that the	13
highly pathogenic porcine reproductive	13
in vivo and in	13
pcr products were analyzed	13
of genome and proteome	13
the endou activity of	13
wt jhmv and srr	13
likely due to the	13
cells infected with rtgev	13
of leader rna sequences	13
it is tempting to	13
reading frame of the	13
with the plasma membrane	13
balb c mice were	13
were shown to be	13
porcine transmissible gastroenteritis coronavirus	13
is similar to that	13
the e and m	13
from the coronavirus group	13
c virus core protein	13
have demonstrated that the	13
acid substitutions in the	13
the ts mutant s	13
in hepatitis c virus	13
well tissue culture plates	13
and data not shown	13
analyzed by agarose gel	13
t cells were transfected	13
found in the online	13
it is not known	13
conserved features of genome	13
assembly of the head	13
the absence or presence	13
supernatants were collected and	13
the transmissible gastroenteritis virus	13
play important roles in	13
the transcription initiation site	13
it is difficult to	13
the coronavirus group lineage	13
this research was supported	13
mice infected with the	13
the cell surface and	13
of interferon regulatory factor	13
of e protein in	13
northern blot analysis of	13
of the virus to	13
as an internal control	13
the alb ts icv	13
and influenza c virus	13
proteins were detected by	13
for h followed by	13
were used to infect	13
have previously shown that	13
of the plasma membrane	13
cells were infected at	13
necrotic mosaic virus rna	13
rna was extracted and	13
of coronavirus mouse hepatitis	13
was inserted into the	13
in an in vitro	13
with the fact that	13
open reading frame a	13
off from the coronavirus	13
could not be detected	13
were introduced into the	13
cells were harvested at	13
cell surface expression of	13
the absence of a	13
carried out in a	13
mice at day p	13
and then incubated with	13
activity of prrsv helicase	13
of the head of	13
de groot et a	13
infectious bronchitis virus in	13
replication of mouse hepatitis	13
due to the absence	13
region of the genome	13
of the porcine epidemic	13
of peste des petits	13
washed once with pbs	13
sense rna genome of	13
the online version at	13
also been reported to	13
the nature of the	13
in cell culture and	13
subjected to western blot	13
and conserved features of	13
were carried out as	13
these results show that	13
most closely related to	13
in the sam binding	13
products were digested with	13
resuspended in ml of	13
a final volume of	13
of the jhm strain	13
amino acid sequence analysis	13
was supported in part	13
syndrome coronavirus envelope protein	13
at various time points	13
the subcellular localization of	13
clinical isolates of hcov	13
the helicase activity of	13
to be essential for	13
l a t a	12
that synthesizes bacteriophage t	12
incubated in the presence	12
we have demonstrated that	12
the porcine transmissible gastroenteritis	12
the identification of a	12
on recombinant vaccinia virus	12
a low level of	12
cells were washed and	12
this indicates that the	12
murine coronavirus gene encoding	12
differences between the two	12
at the indicated time	12
as a positive control	12
based on recombinant vaccinia	12
were then incubated with	12
cells were lysed and	12
fusion activity of the	12
of structural proteins during	12
shown on the left	12
in the spinal cord	12
sars coronavirus e protein	12
expressed on the cell	12
room temperature for h	12
of porcine circovirus type	12
vaccinia virus that synthesizes	12
mutations were introduced into	12
cells present within the	12
at the cleavage site	12
c virus rna replication	12
at the codon position	12
inactivated fetal bovine serum	12
for the formation of	12
in viral rna synthesis	12
cells were lysed with	12
it has also been	12
a consequence of the	12
was cloned into the	12
virus that synthesizes bacteriophage	12
the p position of	12
appears to be a	12
characterization of severe acute	12
of the protein in	12
is an enveloped virus	12
and sequence analysis of	12
the s genome segment	12
was due to the	12
the mechanisms by which	12
a t rna polymerase	12
used as templates for	12
at the beginning of	12
be responsible for the	12
titer was determined by	12
of the mouse hepatitis	12
the parental jhm virus	12
is tempting to speculate	12
was not due to	12
infected cells and in	12
the cytoplasm of infected	12
be the result of	12
the life cycle of	12
are indicated on the	12
amino acid residues in	12
were isolated from the	12
of the coronaviridae family	12
orfs a and b	12
den boon et al	12
of the role of	12
is believed to be	12
the expression level of	12
jhm strain of mhv	12
cleavage of the e	12
of nsp and nsp	12
of the m protein	12
the mhv s protein	12
cells at h post	12
a grant from the	12
respiratory syndrome coronavirus a	12
our results showed that	12
recombinant vaccinia virus that	12
that of the wild	12
were incubated with a	12
are listed in table	12
an important role for	12
we conclude that the	12
different regions of the	12
for the treatment of	12
for the induction of	12
predicted amino acid sequences	12
results demonstrate that the	12
is essential for virus	12
the in vitro translation	12
of the complement system	12
system based on recombinant	12
a nested set of	12
to note that the	12
icv at an moi	12
the difference in the	12
in the viral envelope	12
this could be due	12
that most of the	12
the viral genome and	12
glycoprotein of murine coronavirus	12
does not appear to	12
is probably due to	12
member of the coronaviridae	12
coronavirus gene encoding the	12
cleavage of structural proteins	12
pcr products were cloned	12
the rough endoplasmic reticulum	12
the absence of the	12
s or s pn	12
were incubated for min	12
can be detected in	12
cells were plated in	12
of a number of	12
e glycoprotein of murine	12
end of the genomic	12
the he protein of	12
the amino acid residues	12
with an equal volume	12
a mouse model of	12
cdna copy of the	12
was analyzed by western	12
a mouse hepatitis virus	12
rat alveolar type i	12
in view of the	12
the expression of a	12
the polymerase chain reaction	12
detected by western blot	12
synthesizes bacteriophage t rna	12
animal care and use	12
cells infected with wt	12
presence and absence of	12
at the indicated times	12
was added to a	12
we demonstrated that the	12
with fmdv o campos	12
normalized to that of	12
the course of infection	12
length cdna clone of	12
spike protein of sars	12
was similar to the	12
group f rna mutants	12
hepatitis c virus ns	12
the infected cells were	12
data indicated that the	12
mock infected or infected	12
of the expected size	12
of the recombinant viruses	12
one possibility is that	12
of the mhv genome	11
are likely to be	11
have been implicated in	11
structure and function of	11
are presented as the	11
enzyme is a functional	11
for the sars coronavirus	11
type i ifn signaling	11
for h in the	11
central nervous system of	11
and h n viruses	11
the presence of an	11
molecular weight of kda	11
type i ifn production	11
the protein encoded by	11
receptor for the sars	11
spike protein is a	11
the production of infectious	11
studies are needed to	11
western blot analysis with	11
of b and ab	11
of simian virus large	11
length infectious cdna of	11
has been studied in	11
a significant reduction in	11
may contribute to the	11
the n protein is	11
determine the role of	11
were used in the	11
of bamboo mosaic virus	11
of the porcine transmissible	11
of human coronavirus oc	11
an increase in the	11
viral titer was determined	11
as shown in table	11
the production of type	11
molecular weight of the	11
in the levels of	11
mrna was detected in	11
results show that the	11
the cells were treated	11
a region of the	11
is similar to the	11
involved in the regulation	11
were not detected in	11
were amplified by pcr	11
of each of the	11
more closely related to	11
these results are consistent	11
analyzed by western blotting	11
protein in coronavirus assembly	11
region of the coronavirus	11
is required for virus	11
the legend to fig	11
pcr was carried out	11
structural proteins during the	11
infectious bronchitis virus c	11
obtained from the american	11
not essential for viral	11
at the restrictive temperature	11
the center of the	11
genomic and subgenomic rnas	11
the first nucleotides of	11
to determine the effect	11
the presence and absence	11
previous studies have shown	11
regulation of bnip gene	11
the mouse hepatitis virus	11
control of the t	11
cytoplasm of infected cells	11
the etiological agent of	11
a significant role in	11
the n protein and	11
institute of allergy and	11
were incubated for hr	11
with porcine reproductive and	11
due to the presence	11
simian hemorrhagic fever virus	11
of the negative strand	11
studies are required to	11
fractions were collected from	11
aids research and reference	11
mhv e protein ion	11
were transfected with plasmids	11
have been associated with	11
the leader rna and	11
cells were obtained from	11
to those of the	11
cause of severe acute	11
early stages of infection	11
was measured at nm	11
at the surface of	11
epidermal growth factor receptor	11
immunodeficiency virus type envelope	11
and phylogenetic analysis of	11
genome sequence of a	11
the transmembrane domain of	11
were used as the	11
of bnip gene expression	11
investigate the role of	11
in the murine coronavirus	11
our data suggest that	11
and washed three times	11
treatment of cells with	11
determine the effect of	11
terminal end of the	11
s subunit of the	11
this study was supported	11
pbs and incubated with	11
at the site of	11
m and n proteins	11
has been reported in	11
of the wild type	11
the small di rna	11
and its role in	11
study was supported by	11
performed according to the	11
it has been proposed	11
replication in cultured cells	11
in vitro transcribed rna	11
high levels of viral	11
tomrsv rna and rna	11
respiratory syndrome virus in	11
we are grateful to	11
were subjected to immunoprecipitation	11
of balb c mice	11
in ergic golgi membranes	11
the formation of a	11
in response to infection	11
was carried out by	11
compared to the parental	11
and incubated for hr	11
national institute of allergy	11
are not essential for	11
results are consistent with	11
viral rna was extracted	11
a major role in	11
incubated at for min	11
moloney murine leukemia virus	11
were incubated with the	11
from the nucleus to	11
stained with ethidium bromide	11
of type i ifns	11
cells as compared to	11
in the presence and	11
hela cells expressing the	11
of the structural proteins	11
cells and pam cultures	11
and is required for	11
one set of infected	11
the copy number of	11
in the viral genome	11
centrifugation for min at	11
were present in the	11
for min in the	11
and herpes simplex virus	11
of p and p	11
entry into target cells	11
the coronavirus avian infectious	11
it remains to be	11
mhv infection of the	11
set of infected cells	11
the reaction was stopped	11
by pcr using the	11
paraformaldehyde for min at	11
to determine the role	11
as described in fig	11
helicase activity of ddx	11
in viral rna replication	11
of infected cells and	11
consistent with our previous	11
was present in the	11
have been identified as	11
viral rna synthesis and	11
analysis was performed using	11
the beginning of the	11
cd t cell responses	11
is not present in	11
and the absence of	11
cov s or s	11
an equal amount of	11
essential for virus replication	11
an essential role in	11
a novel coronavirus from	11
infection with h n	11
of barley stripe mosaic	11
at a ratio of	11
to play a role	11
by western blot using	11
the ratio of the	11
to the ability of	11
the pol zn site	11
targeted to the golgi	11
the differences in the	11
well plates were infected	11
lysates were prepared and	11
the head of bacteriophage	11
is present in the	11
infectious cdna clone of	11
proteins during the assembly	11
monoclonal antibodies specific for	11
top of the gradient	11
have been isolated from	11
at the level of	11
mhc class i and	11
the morphology of the	11
inoculum was removed and	11
plays a critical role	11
during the assembly of	11
the inoculum was removed	11
least three independent experiments	11
both ends of the	11
was amplified by rt	11
of allergy and infectious	11
of tobacco mosaic virus	11
of the coronavirus avian	11
confirmed by dna sequencing	10
in this study are	10
we did not observe	10
the plasma membrane of	10
the p and p	10
of two murine coronaviruses	10
the brain and spinal	10
the intensity of the	10
by mouse hepatitis virus	10
translation of orf b	10
has not yet been	10
studies have demonstrated that	10
in the northern territory	10
research and reference reagent	10
by polymerase chain reaction	10
type of viral glycoprotein	10
order to determine the	10
of lipid rafts in	10
and sequencing of the	10
results are shown in	10
on the day of	10
this result indicates that	10
exclude the possibility that	10
were incubated at c	10
in the endoplasmic reticulum	10
was derived from the	10
the golgi complex and	10
in the serum of	10
of bamboo mosaic potexvirus	10
have the potential to	10
is targeted to the	10
the presence of excess	10
the heptad repeat region	10
one or more of	10
with the virion envelope	10
twice with pbs and	10
membrane permeabilizing activity of	10
and replication of the	10
protein of influenza a	10
to murine hepatitis virus	10
characterization of the coronavirus	10
g a and rmv	10
head of bacteriophage t	10
in supplementary table s	10
to the number of	10
compared with that of	10
of the genome and	10
no significant difference in	10
the effects of the	10
a high degree of	10
showed the presence of	10
plasmid reverse genetics system	10
of the international committee	10
the cytoplasmic tails of	10
in the perinuclear region	10
to a lesser extent	10
of coronavirus e protein	10
the first and second	10
the host immune response	10
the loss of the	10
the pellet was resuspended	10
transcription and replication of	10
from the plasma membrane	10
we cannot rule out	10
between viral envelope and	10
the genomic rna and	10
the transfected cells were	10
of basic amino acids	10
of the active site	10
the replication of the	10
is a type i	10
by a grant from	10
could be used to	10
from santa cruz biotechnology	10
has been associated with	10
in vitro transcripts of	10
the complete sequence of	10
been shown to interact	10
and functional analysis of	10
the recombinant vaccinia virus	10
isolation of a novel	10
ha g packaging into	10
essential for virus infectivity	10
proteases and rna polymerase	10
the nucleus and the	10
of the presence of	10
to the detection of	10
sequence of the genomic	10
with the presence of	10
two sets of dbt	10
college of veterinary medicine	10
and inserted into the	10
these data suggested that	10
the sizes of the	10
to rule out the	10
the transmissible gastroenteritis coronavirus	10
type i interferon production	10
response to viral infection	10
infected with the recombinant	10
were removed from the	10
the early stages of	10
subgenomic mrna transcription in	10
used as a positive	10
cd c cells within	10
are known to be	10
hydrogen bond to the	10
the hcv core protein	10
has been observed in	10
of african horse sickness	10
of the coronavirus ibv	10
interaction with the viral	10
species of two murine	10
incubated at the non	10
the number of cells	10
to the instructions of	10
carrying mutations in the	10
was performed according to	10
of e and m	10
the national institute of	10
compared to that in	10
was calculated as the	10
from the central nervous	10
from the endoplasmic reticulum	10
the cns of mice	10
like particles by co	10
type i cell cultures	10
genetics system for the	10
mouse cell line dbt	10
the transcription start site	10
did not bind to	10
the erk signaling pathway	10
a recombinant vaccinia virus	10
d n and e	10
were infected with jev	10
in this study were	10
preferential requirement of p	10
with a molecular weight	10
of the overlapping genes	10
in the range of	10
part of the protein	10
analysis showed that the	10
is not yet known	10
mouse hepatitis virus infection	10
human enteroviruses and rhinoviruses	10
apparent molecular mass of	10
was performed in a	10
proteins were analyzed by	10
has been demonstrated to	10
from the brains of	10
that the s protein	10
was not affected by	10
the results indicated that	10
fusion of the viral	10
confirmed the presence of	10
was dependent on the	10
the result of a	10
infected with fmdv o	10
our results indicate that	10
after the addition of	10
blot analysis of the	10
were purchased from the	10
by public health service	10
total rna extracted from	10
in the loss of	10
has been previously described	10
sequence of the genome	10
it is noteworthy that	10
receptor for advanced glycation	10
has been detected in	10
the n protein was	10
and analyzed by electrophoresis	10
the presence of two	10
there was no detectable	10
of the rv g	10
the brains of infected	10
has been suggested to	10
the data presented in	10
and the mixture was	10
in the process of	10
the influenza virus hemagglutinin	10
the lack of a	10
passages in tissue culture	10
then added to the	10
and reference reagent program	10
supplemented with fetal calf	10
has also been observed	10
in the inhibition of	10
the first time that	10
used as template for	10
reverse transcription was performed	10
supernatants were collected at	10
of at least three	10
the absence of selection	10
pcr products were digested	10
is in contrast to	10
n pdm influenza infection	10
n and e q	10
largest rna virus genome	10
strains of porcine reproductive	10
the side chain of	10
both the a and	10
page and transferred to	10
the results of the	10
in this paper we	10
large di rna species	10
has been implicated in	10
putative proteases and rna	10
cloning and sequencing of	10
to play an important	10
cd t cells in	10
the significance of the	10
influenza a virus m	10
c cells within the	10
of the order nidovirales	10
our data indicated that	10
the presence of rna	10
respiratory syndrome coronavirus nucleocapsid	10
in the level of	10
atg and ns b	10
the group f rna	10
using the rneasy mini	10
with hematoxylin and eosin	10
all three p proteins	10
with pbs and incubated	10
the medium was replaced	10
t cell responses in	10
used to determine the	10
it was of interest	10
were then washed three	10
translation to rna replication	10
of the envelope glycoprotein	10
was amplified by pcr	10
these data demonstrated that	10
infectious virus could be	10
the mutant s protein	10
and subcellular localization of	10
were analyzed by electrophoresis	10
murine mammary tumor virus	10
in order to evaluate	10
the fusion activity of	10
with paraformaldehyde for min	10
fold molar excess of	10
the small envelope protein	10
did not appear to	10
leader rna and the	10
the number of the	10
antibody was used to	10
care and use committee	10
agarose gel electrophoresis and	10
cov spike protein in	10
mutations in the sam	10
according to the instructions	10
is the first report	10
infected at an moi	10
the viral replication complex	10
cells in the absence	10
the level of virus	10
of cells infected with	10
with paraformaldehyde in pbs	10
the extent of the	10
terminal half of nsp	10
a wide variety of	10
vero cells were grown	10
and boiled for min	10
of a neurotropic coronavirus	10
were washed with phosphate	10
of the coat protein	10
play critical roles in	10
to the lack of	10
expression of the he	10
is a receptor for	10
has been identified as	10
plaque assays were performed	10
was detected with a	10
in the rbd of	10
protein with the virion	10
cells were grown to	10
of rna and rna	10
is derived from the	10
of the orf a	10
extracts were immunoprecipitated with	10
in the coding region	10
lysed in ripa buffer	10
was confirmed by western	10
a reverse genetics system	10
of citrus tristeza virus	10
were transferred to a	10
the porcine epidemic diarrhea	10
cleavage sites of the	10
multifunctional regulator of expression	10
of peptide h on	10
a novel type of	10
the rneasy mini kit	10
the plates were incubated	10
according to the method	10
the instructions of the	10
are summarized in table	10
following mhv infection of	10
be related to the	10
these data indicated that	10
of the t promoter	10
s glycoprotein gene of	10
protein of porcine reproductive	10
the molecular weight of	10
than those of the	10
the ibv e and	10
of coronavirus infectious bronchitis	10
define the viral glycoprotein	9
a man with pneumonia	9
structure and genome expression	9
intracellular rna species of	9
the mrna of the	9
turnip yellow mosaic virus	9
characterization of a coronavirus	9
were subjected to sds	9
and identification of the	9
was consistent with the	9
by hepatitis c virus	9
both in vitro and	9
studies suggest that the	9
centrifuged at g for	9
the rous sarcoma virus	9
and proteome of sars	9
followed by a secondary	9
used in these experiments	9
it is unclear whether	9
of mutations in the	9
total cellular rna was	9
of virus replication in	9
rnas were extracted from	9
the absence of rna	9
of the heptad repeat	9
and the ability to	9
membrane fusion activity of	9
in most of the	9
the right of the	9
were analyzed by agarose	9
dmem supplemented with fcs	9
initiation site and the	9
by western blotting with	9
in viral entry and	9
a conformational change in	9
with pneumonia in saudi	9
as the reciprocal of	9
class i viral fusion	9
of c for s	9
data presented in this	9
under the same conditions	9
in the brain of	9
to a nitrocellulose membrane	9
respiratory syndrome coronavirus nsp	9
e gene mutants of	9
centrifuged for min at	9
in the di rna	9
of the viral proteins	9
stained with hematoxylin and	9
from a man with	9
gl and g glycoproteins	9
a and b are	9
not essential for virus	9
to the activation of	9
protein is critical for	9
the spike gene of	9
virus genome as an	9
novel coronavirus from a	9
would be expected to	9
cells were overlaid with	9
that the amino acid	9
the results of this	9
the limit of detection	9
the presence of virus	9
is an integral membrane	9
gene mutants of mouse	9
on the plasma membrane	9
were then transferred to	9
was performed by the	9
in a co incubator	9
virus confirms a pivotal	9
are shown on the	9
the observation that the	9
were obtained from dr	9
the phosphorylation status of	9
of entry into the	9
inhibition of erk activation	9
were similar to those	9
supported by the national	9
the cbp degradation by	9
analysis was carried out	9
molecular mass of the	9
treated with dnase i	9
is not necessary for	9
of brome mosaic virus	9
the viral glycoprotein responsible	9
cells as previously described	9
ribv b and ribv	9
at any time point	9
the ability of these	9
determined by plaque assays	9
that b protein is	9
is capable of inducing	9
production of type i	9
with dmem supplemented with	9
suppression of type i	9
mice were infected with	9
and the supernatants were	9
of the viral replicase	9
we were interested in	9
the left indicate molecular	9
for severe acute respiratory	9
has not been determined	9
of the complete nucleotide	9
enveloped virus with a	9
infected cells was shifted	9
fixed with paraformaldehyde in	9
cl pro inhibitor complex	9
the host innate immune	9
virus in cultured cells	9
used as a control	9
despite the fact that	9
from the sg start	9
for attachment and cell	9
encoding the putative proteases	9
in vitro models of	9
between the nucleus and	9
were analyzed by western	9
there was no significant	9
no effect on the	9
have been used to	9
in c bl mice	9
genome of the coronavirus	9
is illustrated in fig	9
e protein in the	9
cells were fixed at	9
of the helicase protein	9
as compared to ccl	9
protein is responsible for	9
at the top of	9
to be able to	9
incubated for h with	9
of the arterivirus replicase	9
t cell responses to	9
are present in the	9
of a new human	9
was supported by national	9
of vero e cells	9
by use of a	9
coronavirus ribosomal frameshifting signal	9
for the s protein	9
t cells in the	9
centrifuged at rpm for	9
of this study was	9
class i fusion proteins	9
bronchitis virus a polyprotein	9
rounds of plaque purification	9
the amino acid diversity	9
mouse hepatitis virus confirms	9
by inhibition of the	9
of the hepatitis c	9
of puuv and tulv	9
the study of the	9
from three independent experiments	9
infectious bronchitis virus and	9
of constructed e gene	9
presented as the mean	9
infected with h n	9
the authors would like	9
expressed on the surface	9
the early stage of	9
is conserved in all	9
in the viral life	9
able to replicate in	9
an alignment of the	9
is dependent on the	9
attenuated in vitro and	9
subcellular localization of the	9
and c for min	9
of the immune response	9
the increase in the	9
b and ribv ab	9
independent assembly of coronavirus	9
viral glycoprotein responsible for	9
in the genomic rna	9
a small number of	9
immediately upstream of the	9
required for virus replication	9
monoclonal antibodies to jhm	9
were inserted into the	9
under a fluorescence microscope	9
cv y cdna clones	9
the genome of mhv	9
ml of s methionine	9
s and s subunits	9
the coronavirus e protein	9
the first orf of	9
man with pneumonia in	9
for e protein in	9
the synthesis of sg	9
allergy and infectious diseases	9
the standard jhm virus	9
reaction was stopped by	9
that it may be	9
the reciprocal of the	9
replication of porcine reproductive	9
the construction of the	9
results shown in fig	9
of amino acid substitutions	9
mice were immunized with	9
compared with mock infected	9
the ha stem region	9
of the avian coronavirus	9
responsible for attachment and	9
in vitro translation products	9
were derived from the	9
rpm for min at	9
the putative proteases and	9
and the amount of	9
rna and proteins from	9
research was supported by	9
cells infected with mhv	9
model of multiple sclerosis	9
media were replaced with	9
products were extracted with	9
order to determine whether	9
fusion between viral envelope	9
of the nlrp inflammasome	9
when compared with the	9
respiratory syndrome coronavirus replicase	9
constructed e gene mutants	9
the nucleotide sequences of	9
wild type and ccl	9
added to the culture	9
between tgbp and tgbp	9
the mixture was incubated	9
the rbd of s	9
culture supernatants were collected	9
coding region of the	9
was supported by public	9
acute respiratory syndrome the	9
and proteolytic processing in	9
been shown to have	9
were infected at a	9
will be interesting to	9
and nomenclature of viruses	9
in the secretory pathway	9
cells was shifted to	9
substitutions at the codon	9
cells transfected with pmh	9
and in vitro models	9
t cells transfected with	9
of the di rna	9
national multiple sclerosis society	9
did not show any	9
cycles of c for	9
the presence of both	9
were washed in pbs	9
the number of amino	9
influenza virus m protein	9
a comparison of the	9
bovine viral diarrhea virus	9
absence of selection pressure	9
has been proposed that	9
advanced glycation end products	9
confirms a pivotal role	9
in viral replication and	9
be used as a	9
the e protein ic	9
of nsp with nsp	9
of the p proteins	9
the influenza a virus	9
was carried out for	9
supported by public health	9
total rna was isolated	9
transferred to a nitrocellulose	9
antibodies to murine hepatitis	9
is not due to	9
have a role in	9
type i cells were	9
by the coronavirus infectious	9
moi of pfu cell	9
of n protein was	9
to the formation of	9
in the lung tissue	9
analysis of constructed e	9
the hepatitis b virus	9
recombinant viruses derived from	9
of the interaction between	9
our data indicate that	9
nested set of subgenomic	9
at the p position	9
be detected in the	9
demonstrates that the spike	9
have been previously described	9
monoclonal antibodies to murine	9
in the cytoplasm and	9
binding domain of the	9
by porcine epidemic diarrhea	9
infected cells at h	9
for h at jc	9
response to infection with	9
glycoprotein responsible for attachment	9
and subjected to western	9
by centrifugation at rpm	9
hepatitis virus confirms a	9
the overlapping genes under	9
the nucleocapsid protein n	9
the regulation of the	9
it was noted that	9
role for e protein	9
the hr main chain	9
the cells were stained	9
the murine coronavirus mouse	9
primary structure of the	9
in vitro assembly of	9
porcine epidemic diarrhoea virus	9
expression of viral envelope	9
have been detected in	9
of the viral rna	9
the active site of	9
to each of the	9
with extensive destruction of	9
using monoclonal antibodies to	9
in g g phase	9
cleavage of the s	9
to the golgi apparatus	9
type i fipv black	9
and washed twice with	9
to the loss of	9
this study was to	9
genomic and subgenomic rna	9
was performed by using	9
for min to remove	9
institutional animal care and	9
buffer for h at	9
analysis of the porcine	9
at the concentration of	9
pelleted by centrifugation at	9
for virus replication in	9
the number of infected	9
infection of vero cells	9
extensive destruction of myelin	9
to the identification of	9
and simian hemorrhagic fever	9
were clarified by centrifugation	9
on the ability of	9
the substrate specificity of	9
the results shown in	9
has been described for	9
to further characterize the	9
transfected with plasmids encoding	9
might be involved in	9
entry into the kennel	9
it is not surprising	9
infected cells were labeled	9
was carried out at	9
translation and rna replication	9
proteins were transferred to	9
were washed twice in	9
the coronavirus spike protein	9
was used as template	9
the development of an	9
cell lysates were subjected	9
cytoplasmic tail of the	9
the total amount of	9
as a consequence of	9
the nucleic acid binding	9
fulfilled for sars virus	9
centrifugation at g for	9
that corresponds to the	9
coronavirus cl pro s	9
a better understanding of	9
and incubated at for	9
in severe acute respiratory	9
have also been shown	9
representative of at least	9
pivotal role for e	9
of the structure of	9
three times in pbs	9
a final extension at	9
have not yet been	9
with pbs and fixed	9
proteolytic processing in the	9
postulates fulfilled for sars	9
in blocking buffer for	9
that the prrsv e	9
a role in viral	9
with icwt or nsp	9
the amount of viral	9
as compared to that	9
was of interest to	9
samples were subjected to	9
analysis revealed that the	9
were washed five times	9
a high level of	9
pneumonia in saudi arabia	9
rna species of two	9
nucleus and the cytoplasm	9
detected in the cns	9
was used in the	9
have an effect on	9
sets of dbt cells	9
membrane was incubated with	9
clearance of mouse hepatitis	9
recombinant vesicular stomatitis virus	9
that the n protein	9
analysis using monoclonal antibodies	9
of the s and	9
syndrome coronavirus nucleocapsid protein	9
a pivotal role for	9
the lumen of the	9
were used in this	9
authors would like to	9
replication of hepatitis c	9
coronavirus from a man	9
may account for the	9
of the orf b	9
were fixed and stained	9
c protein with the	9
simian virus large tumor	9
an important role of	9
agent of severe acute	9
not appear to be	9
has been proposed to	9
results are representative of	9
to the fact that	9
by a mouse hepatitis	9
are shown in table	9
were generated using the	9
the positions of the	9
the s protein and	9
the absence of other	9
plates were incubated at	9
showed the detection of	9
were unable to detect	9
in the p position	9
the upper respiratory tract	9
monoclonal antibodies to the	9
a severe acute respiratory	9
transmissible gastroenteritis coronavirus genome	9
the growth properties of	9
surface expression of the	9
the integrity of the	9
of viral envelope protein	9
viral envelope protein genes	9
coronavirus e protein forms	9
may play an important	8
early stages of the	8
rna was detected in	8
were not able to	8
rnase a treatment of	8
results are presented as	8
molecular mass of kda	8
number of amino acid	8
template for subgenomic mrna	8
to assess the role	8
is due to the	8
uranyl acetate and lead	8
host innate immune responses	8
in the order nidovirales	8
for in vitro transcription	8
localization of the sequence	8
proteins encoded by the	8
peripheral blood mononuclear cells	8
cd t cells and	8
the brains of mhv	8
specific intracellular rna species	8
cells were cotransfected with	8
described in this report	8
b region of the	8
once with pbs and	8
in agreement with our	8
other cov n proteins	8
host innate immune response	8
we showed that the	8
is the first study	8
domain of bamv replicase	8
in minimal essential medium	8
cd t cells are	8
detected in infected cells	8
the interface between and	8
regions of secondary structure	8
cells infected with a	8
been observed in the	8
as the percentage of	8
the early phase of	8
on the synthesis of	8
causing disseminated encephalomyelitis with	8
whole cell lysates were	8
dependent rna polymerase activity	8
no infectious virus was	8
disseminated encephalomyelitis with extensive	8
p and p positions	8
for h before inoculating	8
with the viral nucleocapsid	8
the host range of	8
would be interesting to	8
hepatitis virus strain jhm	8
viruses and their replication	8
has been demonstrated in	8
before inoculating with fipv	8
is unlikely to be	8
results strongly suggest that	8
was found to have	8
the leader sequence of	8
to determine if there	8
the biological significance of	8
from the rer to	8
e protein ic activity	8
of the standard jhm	8
a mouse monoclonal antibody	8
genetic localization of the	8
cells that had been	8
sequences of the two	8
with various concentrations of	8
three bsmv sgrna promoters	8
findings are consistent with	8
the high degree of	8
the relationship between the	8
were maintained in dmem	8
a kind gift from	8
for the activation of	8
visualized by staining with	8
samples were collected from	8
of the p mapk	8
the murine coronavirus genome	8
to the development of	8
identified in infected cells	8
and cd t cell	8
cloning and sequence analysis	8
as in the case	8
cells were subjected to	8
is likely due to	8
type i cells in	8
the protein in the	8
of other viral proteins	8
human coronavirus strain oc	8
sequence of mouse hepatitis	8
it appears that the	8
the cells were lysed	8
s and c for	8
a murine coronavirus in	8
coronavirus spike protein is	8
wild type and ribv	8
for s and c	8
supported by a grant	8
two different k cleavage	8
to investigate the role	8
protein in infected cells	8
ion channels in planar	8
has been found to	8
association of the infectious	8
to the expression of	8
cannot rule out the	8
the pathogenicity of the	8
of potato virus x	8
r l mutant viruses	8
the membranes were washed	8
influenza a and b	8
by targeted rna recombination	8
aminopeptidase n is a	8
activation of the p	8
an enveloped virus with	8
der hoek et al	8
from the northern territory	8
in order to identify	8
as the template for	8
cov e protein is	8
the membrane was washed	8
the number of plaques	8
glycoproteins and interaction with	8
in influenza a virus	8
is considered to be	8
has led to the	8
product was digested with	8
van der hoek et	8
from translation to rna	8
were separated on a	8
the rnase protection assay	8
the surface expression of	8
viral infection of the	8
pathogenic avian influenza viruses	8
the level of the	8
were rinsed three times	8
a decrease in the	8
old c bl mice	8
effect on viral replication	8
for mouse hepatitis virus	8
mouse hepatitis virus the	8
porcine hemagglutinating encephalomyelitis virus	8
do not appear to	8
in the mhv s	8
binding and membrane fusion	8
mrna of mouse hepatitis	8
mapping of the coronavirus	8
were collected by centrifugation	8
were performed using the	8
the green fluorescent protein	8
were probed with a	8
immunoprecipitates were analyzed by	8
of two different k	8
were infected with ibv	8
mediated cleavage of c	8
an in vitro system	8
the properties of the	8
replication of viral rna	8
the membrane was incubated	8
described in the text	8
enhanced by pveer expression	8
for the evaluation of	8
activity of virions by	8
with the exception that	8
which has been shown	8
the three bsmv sgrna	8
systematic assembly of a	8
into the cns of	8
an early stage of	8
the interaction of the	8
similar to those observed	8
out the possibility of	8
virus titers in the	8
of demyelination induced by	8
are members of the	8
different k cleavage fragments	8
was also observed in	8
data are representative of	8
cells were extensively washed	8
and the fact that	8
assess the role of	8
cv tumor cells were	8
the genomic rna is	8
epidemic diarrhea virus in	8
critical reading of the	8
were then used to	8
have the ability to	8
with bovine serum albumin	8
be interesting to determine	8
was responsible for the	8
experiments were carried out	8
be of interest to	8
c cl pro s	8
the large di rnas	8
induced p mapk activation	8
membranes were incubated with	8
of the leader rna	8
has not been identified	8
respiratory syndrome virus and	8
been suggested to be	8
virus e protein is	8
region of the a	8
the amount of the	8
does not contain a	8
and r l mutant	8
the first nt of	8
transported to the cell	8
infected with pedv at	8
processing in the nidovirales	8
completion of the sequence	8
encephalomyelitis with extensive destruction	8
was not present in	8
we would like to	8
s and s genome	8
to be responsible for	8
and severe acute respiratory	8
were approved by the	8
fixed and stained with	8
separation of two different	8
cells incubated at the	8
the coronavirus s protein	8
the viruses and their	8
the origin of the	8
amino acid substitution in	8
and separation of two	8
were added to each	8
were infected with fmdv	8
the release of the	8
over a period of	8
h before inoculating with	8
cells were trypsinized and	8
entry into host cells	8
the presence of viral	8
nih aids research and	8
as the negative control	8
cavv cl pro inhibitor	8
length infectious cdna clone	8
could be explained by	8
of cells were infected	8
out as described previously	8
a consequence of a	8
tissue culture infectious dose	8
infected cells incubated at	8
added to a final	8
was normalized to that	8
of the di rnas	8
untranslated region of the	8
the day of entry	8
the mmessage mmachine t	8
plus strand rna viruses	8
the viral replication cycle	8
dependent on the presence	8
and determination of its	8
from the gene polyprotein	8
on the virion surface	8
within the cln of	8
were infected with pedv	8
t cell activation and	8
antibodies against ibv n	8
the cell monolayers were	8
of the leader sequence	8
was supported by nih	8
to be important in	8
for the loss of	8
of the replication of	8
infected cells were incubated	8
pellets were suspended in	8
was not required for	8
not due to the	8
prior to incubation with	8
of the fusion peptide	8
by northern blot analysis	8
replication and expression of	8
and function of the	8
reading of the manuscript	8
each of the four	8
the antiviral activity of	8
at room temperature and	8
was also detected in	8
grown in the presence	8
for the role of	8
indicate molecular masses in	8
cells was determined by	8
interaction of nsp with	8
on the observation that	8
in the s subunit	8
of cells expressing the	8
the he genes of	8
channels in planar lipid	8
assay was performed using	8