trigram

This is a table of type trigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

trigram	frequency
the presence of	769
mouse hepatitis virus	515
severe acute respiratory	474
acute respiratory syndrome	466
data not shown	408
in the presence	407
as well as	397
respiratory syndrome coronavirus	369
the cells were	320
analysis of the	307
were infected with	306
and respiratory syndrome	304
shown in fig	296
porcine reproductive and	294
reproductive and respiratory	294
for min at	281
the absence of	280
in this study	277
for h at	276
cells infected with	274
the expression of	264
according to the	260
respiratory syndrome virus	260
infectious bronchitis virus	252
of mouse hepatitis	245
end of the	243
the role of	241
hepatitis c virus	239
of the s	228
cells were infected	226
the s protein	222
a and b	221
of the viral	217
sequence of the	215
as shown in	207
been shown to	207
as previously described	201
in the absence	200
the number of	196
is required for	194
at room temperature	191
the e protein	191
expression of the	190
it has been	189
was used to	188
in order to	187
human immunodeficiency virus	185
region of the	183
of the virus	182
the cell surface	179
due to the	176
has been shown	169
of the coronavirus	168
to that of	168
of the genome	162
herpes simplex virus	157
transmissible gastroenteritis virus	155
cells were washed	155
of the protein	152
the amount of	151
present in the	148
cd t cells	148
of severe acute	148
as described previously	148
the replication of	148
to determine the	146
the ability of	145
in infected cells	142
based on the	142
consistent with the	142
the effect of	138
of the e	136
cells transfected with	136
the synthesis of	135
was used as	134
the n protein	133
we found that	132
in addition to	131
that of the	131
open reading frame	131
as described above	130
were incubated with	130
one of the	129
the case of	128
materials and methods	126
the level of	124
characterization of the	124
was carried out	124
in the case	123
immunodeficiency virus type	123
was detected in	123
involved in the	123
of the two	123
in contrast to	122
were transfected with	122
wang et al	122
was added to	121
structure of the	120
as compared to	120
the use of	119
amino acid sequence	119
was supported by	118
compared to the	118
at h post	118
of the n	118
the plasma membrane	118
the production of	117
cells were incubated	117
the amino acid	117
of viral rna	116
identification of a	115
of a novel	115
associated with the	114
was determined by	114
to determine if	114
were used to	114
the formation of	113
shown to be	113
li et al	113
in the s	113
derived from the	113
of porcine reproductive	112
in cells infected	112
central nervous system	112
were analyzed by	112
it is possible	111
detected in the	111
showed that the	111
the development of	110
to determine whether	110
porcine epidemic diarrhea	110
and analyzed by	110
suggest that the	110
western blot analysis	109
a role in	109
vesicular stomatitis virus	109
the present study	108
used as a	107
t rna polymerase	107
influenza a virus	107
the s gene	107
similar to that	107
rna was extracted	106
in the cytoplasm	105
this work was	105
found to be	105
of infected cells	104
were treated with	104
is possible that	103
each of the	103
by western blot	103
activity of the	102
the genomic rna	102
infected with the	101
the possibility that	101
in response to	101
of the sars	101
in which the	100
similar to the	100
infected cells and	100
east respiratory syndrome	100
cells were transfected	99
was performed using	99
the fact that	99
is essential for	99
middle east respiratory	98
epidemic diarrhea virus	98
amino acid residues	97
cells were then	97
the murine coronavirus	97
infected cells were	97
of murine coronavirus	97
sequence analysis of	96
results suggest that	96
and characterization of	96
of the human	96
found in the	96
role in the	95
washed three times	95
observed in the	94
members of the	94
in cell culture	94
murine hepatitis virus	94
coronavirus infectious bronchitis	94
a variety of	93
an important role	92
corresponding to the	92
and incubated for	91
demonstrated that the	91
downstream of the	91
for hr at	91
was found to	91
of the murine	90
the ability to	90
comparison of the	90
the majority of	90
added to the	90
the addition of	89
the detection of	89
nucleotide sequence of	89
the viral genome	89
incubated for h	89
results indicate that	89
suggesting that the	89
cells in the	88
liu et al	88
part of the	87
sequences of the	87
amino acid sequences	87
a number of	87
the lack of	86
has been reported	86
was observed in	86
of the mhv	86
three times with	86
of e protein	86
open reading frames	85
in the present	85
suggests that the	85
all of the	85
indicate that the	85
have been shown	84
have shown that	84
activation of the	84
responsible for the	84
characterization of a	83
mice infected with	83
regions of the	83
presence of the	83
the spike protein	83
feline infectious peritonitis	83
for min and	83
domain of the	83
work was supported	83
is associated with	82
is consistent with	82
data suggest that	82
in the same	82
cell lysates were	82
as described in	81
the sequence of	81
the activation of	81
in vitro translation	81
transmissible gastroenteritis coronavirus	80
has also been	80
in vitro and	80
vero cells were	79
fetal bovine serum	79
were detected in	78
the induction of	78
coronavirus spike protein	78
processing of the	78
most of the	78
cells were fixed	78
be due to	78
are shown in	78
in vero cells	78
a novel coronavirus	77
member of the	77
an moi of	77
multiplicity of infection	77
at an moi	77
was confirmed by	77
pcr products were	77
was used for	77
to be a	77
role of the	76
were used as	76
the in vitro	76
it is not	76
has not been	76
e protein is	76
and m proteins	75
cleavage of the	75
dependent rna polymerase	75
transfected with the	75
of the rna	75
the end of	75
appears to be	75
were obtained from	75
in the cns	74
hek t cells	74
were determined by	74
the central nervous	74
the nucleocapsid protein	74
e and m	73
mutations in the	73
is important for	73
were incubated for	73
the severe acute	73
protein in the	72
in the viral	72
indicated that the	72
between the two	72
the importance of	72
these results suggest	72
balb c mice	72
cloned into the	72
with pbs and	72
were washed with	71
presence of a	71
were subjected to	71
used in this	71
were able to	70
to interact with	70
the generation of	70
and subjected to	70
was extracted from	70
cov e protein	70
to the cell	70
differences in the	70
important role in	70
viral rna synthesis	70
min at room	70
h n pdm	69
was shown to	69
is involved in	69
terminus of the	69
of the spike	69
the e glycoprotein	69
when compared to	69
the surface of	69
with respect to	68
zhang et al	68
a member of	68
any of the	68
on the cell	68
resulted in a	68
the effects of	68
the levels of	68
of influenza virus	68
t cells were	68
the host cell	67
indicating that the	67
upstream of the	67
were carried out	67
infection of the	67
could not be	67
of hepatitis c	66
innate immune response	66
cells were treated	66
strand rna viruses	66
hepatitis b virus	66
a multiplicity of	66
coronavirus mouse hepatitis	65
and incubated with	65
by centrifugation at	65
on the other	65
the mechanism of	65
and incubated at	65
wild type and	65
the viral envelope	65
of type i	64
in tissue culture	64
the function of	64
protein is a	64
type i cells	64
s and s	64
chen et al	64
of the three	64
been shown that	64
g for min	63
the size of	63
be involved in	63
equine arteritis virus	63
crystal structure of	63
are required for	63
the course of	63
the pathogenesis of	63
such as the	63
were used for	63
but not in	62
infected with a	62
of viral proteins	62
a mixture of	62
type i interferon	62
the other hand	62
cells were harvested	62
were grown in	62
are involved in	62
the interaction between	62
the results of	61
and in the	61
were inoculated with	61
of the same	61
in cultured cells	61
as described by	61
the infected cells	61
at a multiplicity	61
reverse genetics system	60
in vitro transcription	60
play a role	60
used in the	60
in the nucleus	60
well as the	60
cells were lysed	60
this suggests that	60
c bl mice	60
have been identified	60
e protein in	60
three independent experiments	60
shown that the	60
of influenza a	60
infected cells was	60
of the genomic	59
the control of	59
avian infectious bronchitis	59
washed with pbs	59
than that of	59
cd t cell	59
supported by the	59
the structure of	59
function of the	59
of the gene	59
of the cell	59
lee et al	59
have not been	58
these data suggest	58
vero e cells	58
of the p	58
and in vivo	58
molecular weight of	58
there is a	58
makino et al	58
presence or absence	58
is responsible for	58
type i ifn	58
the m protein	58
s protein was	58
analyzed by sds	58
to study the	58
were incubated at	57
cd and cd	57
human coronavirus e	57
in agreement with	57
relative to the	57
the brains of	57
required for the	57
at c for	57
amino acid substitutions	57
properties of the	57
a series of	57
of transmissible gastroenteritis	57
with severe acute	57
b and c	56
by plaque assay	56
polymerase chain reaction	56
the cns of	56
the endoplasmic reticulum	56
residues in the	56
were found to	56
data indicate that	56
size of the	56
were labeled with	56
or absence of	56
viral rna replication	56
of the porcine	56
of the c	56
hepatitis virus strain	55
the context of	55
the identification of	55
for min in	55
to investigate the	55
of the sequence	55
the genome of	55
mammary tumor virus	55
the regulation of	55
at h p	55
these results indicate	55
similar to those	55
resulted in the	55
huang et al	55
results showed that	55
of human immunodeficiency	55
of n protein	54
is shown in	54
h at c	54
of the ga	54
n protein was	54
are known to	54
cells were grown	54
vitro and in	54
changes in the	54
p and p	54
were observed in	54
the percentage of	54
under materials and	54
in the cell	54
of these viruses	53
alb ts icv	53
for the presence	53
kindly provided by	53
were washed three	53
viral replication and	53
as a control	53
xu et al	53
cavv cl pro	53
the cleavage site	53
protein was detected	52
the sars coronavirus	52
washed twice with	52
not essential for	52
the coronavirus infectious	52
the loss of	52
agarose gel electrophoresis	52
with that of	52
revealed that the	52
of amino acids	52
contribute to the	51
virus in the	51
the s glycoprotein	51
of the influenza	51
found that the	51
were purchased from	51
were stained with	51
was detected by	51
virus replication in	51
interaction between the	51
there was no	51
at the same	51
described under materials	51
isolated from the	51
the presence or	51
analyzed by western	50
obtained from the	50
b and ab	50
suggested that the	50
the virus was	50
contrast to the	50
the active site	50
the s subunit	50
as a template	50
have also been	50
the infectivity of	50
a role for	50
monoclonal antibodies to	50
related to the	50
in the context	50
coronavirus nucleocapsid protein	49
as described under	49
incubated for min	49
not required for	49
used as the	49
the viral rna	49
at the cell	49
under the control	49
interacts with the	49
identification of the	49
west nile virus	49
of the recombinant	49
for h and	49
specific for the	48
were added to	48
porcine transmissible gastroenteritis	48
followed by a	48
subgenomic di rna	48
some of the	48
high levels of	48
t cell responses	48
total rna was	48
specificity of the	48
the interaction of	48
of herpes simplex	48
used for the	48
was obtained from	47
encoded by the	47
we examined the	47
recombinant vaccinia virus	47
replication of the	47
remains to be	47
on the surface	47
e protein was	47
s protein of	47
been reported to	47
located in the	47
the innate immune	47
c for min	47
of the orf	47
of a new	47
of the first	47
was performed with	47
simplex virus type	47
virus infection in	47
the assembly of	47
influenza c virus	47
times with pbs	46
of orf b	46
coronavirus e protein	46
increase in the	46
sequencing of the	46
the e gene	46
and cd t	46
of the a	46
and that the	46
led to the	46
a total of	46
is mediated by	46
snijder et al	46
infected with mhv	46
the wild type	46
virus strain a	46
the n gene	46
with the same	46
infectious peritonitis virus	46
synthesis of the	46
of amino acid	46
of the host	46
to those of	45
h n and	45
appear to be	45
the accumulation of	45
the influenza virus	45
northern blot analysis	45
formation of the	45
gene of the	45
described in the	45
green fluorescent protein	45
in mammalian cells	45
pcr was performed	45
proteins of the	45
cells expressing the	45
was analyzed by	45
in the infected	45
supplemented with fetal	45
the orf b	45
from the national	45
their ability to	45
s glycoprotein gene	45
viral rna was	45
strand rna synthesis	44
virus replication and	44
the result of	44
performed as described	44
protein of the	44
was able to	44
the supernatant was	44
mouse mammary tumor	44
is not known	44
was performed by	44
of porcine epidemic	44
and expression of	44
i i i	44
of the putative	44
s protein is	44
the construction of	44
is not essential	44
addition to the	44
is critical for	44
h at room	44
of the other	44
kim et al	44
were detected by	44
interact with the	44
were maintained in	44
were digested with	44
polyacrylamide gel electrophoresis	44
indicates that the	44
has been described	43
to the nucleus	43
immune response to	43
the extent of	43
the di rna	43
site of the	43
the activity of	43
were immunoprecipitated with	43
for viral replication	43
has been suggested	43
nsp and nsp	43
of the cleavage	43
compared to wild	43
localization of the	43
c and d	43
and in vitro	43
in this paper	43
acid sequence of	43
the golgi complex	43
the observation that	43
the ratio of	43
we show that	43
cells and the	43
due to a	43
for virus replication	43
the release of	43
mhv e protein	42
of s protein	42
likely to be	42
determined by the	42
are consistent with	42
analysis was performed	42
inserted into the	42
to evaluate the	42
that the e	42
rna and rna	42
of the membrane	42
was similar to	42
that it is	42
african swine fever	42
determined by plaque	42
should be addressed	42
is known to	42
to confirm the	42
in viral replication	42
were isolated from	42
be detected in	42
yount et al	42
cells were seeded	42
influenza a viruses	42
is present in	42
is capable of	42
molecular biology of	42
it is likely	42
a result of	42
is the first	42
swine fever virus	42
is likely that	42
did not affect	41
mhc class i	41
was incubated with	41
at h posttransfection	41
the cytoplasm of	41
dbt cells were	41
could be detected	41
the study of	41
proteolytic processing of	41
essential for the	41
portion of the	41
dmem supplemented with	41
were harvested and	41
demonstrate that the	41
interaction with the	41
was performed as	41
and transferred to	41
were then washed	41
and the cells	41
fetal calf serum	41
cells were labeled	41
lu et al	41
referred to as	41
were washed twice	41
is thought to	41
as determined by	41
yang et al	41
of the nucleocapsid	41
jemielity et al	41
binds to the	40
with ml of	40
translation of the	40
position of the	40
infection in the	40
with fetal bovine	40
in the replication	40
were collected and	40
and cloned into	40
evidence for the	40
of viral replication	40
possible that the	40
are associated with	40
of the cells	40
ion channel activity	40
have been reported	40
ability of the	40
the human coronavirus	40
to confirm that	40
the existence of	40
institutes of health	40
was isolated from	40
known to be	40
is dependent on	40
the golgi apparatus	40
blot analysis of	40
has been demonstrated	40
replication in the	40
understanding of the	40
the samples were	40
in the regulation	40
washed once with	39
di rna species	39
the structural proteins	39
the he protein	39
required for efficient	39
ibv e protein	39
were fixed with	39
play an important	39
by the addition	39
of orf a	39
the complement system	39
lin et al	39
final concentration of	39
were cloned into	39
in the first	39
c and c	39
the concentration of	39
of cd t	39
of the predicted	39
because of the	39
as a result	39
control of the	39
in the er	39
of murine hepatitis	39
conjugated goat anti	39
ends of the	39
were incubated in	39
the respiratory tract	39
the leader rna	39
and resuspended in	38
the results showed	38
the leader sequence	38
protein of sars	38
of the sgrna	38
of t cells	38
development of a	38
corse and machamer	38
mhc class ii	38
the molecular biology	38
specific cd t	38
in this report	38
e protein ion	38
gene encoding the	38
have been described	38
the immune response	38
closely related to	38
with wild type	38
on the left	38
the difference in	38
have demonstrated that	38
those of the	38
supernatants were collected	38
the specificity of	38
none of the	38
is able to	38
it is also	38
at for min	38
as a receptor	38
the infected cell	38
together with the	38
provided by dr	38
national institutes of	38
sites of the	37
from infected cells	37
bean mosaic virus	37
the human immunodeficiency	37
protein from the	37
murine coronavirus spike	37
the appearance of	37
to the golgi	37
component of the	37
be important for	37
a receptor for	37
the pcr products	37
of infectious bronchitis	37
assembly of the	37
at an m	37
is not clear	37
in ml of	37
the inhibition of	37
protein with the	37
strand rna virus	37
semliki forest virus	37
components of the	37
transferred to a	37
may be a	37
and stained with	37
is supported by	37
were separated by	37
were found in	37
determine whether the	37
amino acids of	37
protein and the	37
were used in	37
proteins in the	37
to be the	37
the binding of	37
effect on the	37
cell monolayers were	37
by western blotting	37
there is no	37
stranded rna viruses	37
and of the	37
we showed that	37
the a and	37
of infectious virus	37
of at least	37
and cells were	37
rna in the	37
terminal region of	36
were performed using	36
cells were cultured	36
lai et al	36
was found in	36
sg start site	36
terminal domain of	36
in the virus	36
for the first	36
infected vero cells	36
in the lungs	36
of the leader	36
supplemented with fbs	36
infected cells at	36
southern bean mosaic	36
cl pro s	36
by electrophoresis on	36
acid sequences of	36
type and mutant	36
rpm for min	36
of the cns	36
cdna clone of	36
strain of mhv	36
mutational analysis of	36
expression levels of	36
the frequency of	36
a final concentration	36
depending on the	36
to assess the	36
mechanism by which	36
gorbalenya et al	36
this is the	36
deduced amino acid	36
proteolytic cleavage of	36
was digested with	36
of the nsp	36
was performed on	36
simmons et al	36
of human coronavirus	36
of the polymerase	36
cells treated with	36
were performed in	35
the region of	35
transcription start site	35
were present in	35
the efficiency of	35
in this region	35
these data indicate	35
a and c	35
be used to	35
to generate the	35
serves as a	35
rna extracted from	35
the basis of	35
likely due to	35
in virus replication	35
for h with	35
cells were co	35
bovine serum albumin	35
of vero cells	35
show that the	35
terminal half of	35
transfected cells were	35
of sindbis virus	35
expression of a	35
and stored at	35
located at the	35
infected cells in	35
a concentration of	35
then incubated with	35
of wild type	35
was removed and	35
a combination of	35
chang et al	35
we have previously	35
the sg start	35
the cytoplasmic tail	35
cov n proteins	35
were introduced into	35
deletion in the	35
of sars coronavirus	35
of the he	35
be required for	35
the viral replication	35
the location of	35
a wide range	34
was not detected	34
is a member	34
may be due	34
of a single	34
amplified by pcr	34
at h postinfection	34
at the indicated	34
were performed as	34
and the other	34
of these proteins	34
the transmembrane domain	34
the site of	34
of the wild	34
m and n	34
of genomic rna	34
it may be	34
with s methionine	34
were unable to	34
pandemic h n	34
on the basis	34
in the brain	34
wide range of	34
resulting in the	34
to identify the	34
were collected from	34
tank et al	34
added to each	34
performed using the	34
the orf a	34
precipitated with ethanol	34
there was a	34
of the parental	34
were harvested at	34
in the n	34
by using a	34
compared to that	34
is likely to	34
of a coronavirus	34
induced by the	34
the spike glycoprotein	34
in pbs for	34
mutation in the	34
genomic rna of	34
of the mouse	34
majority of the	34
rna was isolated	34
in materials and	34
dependent on the	34
associated with a	34
the ibv e	34
appeared to be	34
all of these	34
innate immune responses	34
by agarose gel	34
wu et al	34
the stability of	34
for detection of	34
it was found	34
results in a	34
at least three	33
the nucleus and	33
h n influenza	33
in cells transfected	33
mouth disease virus	33
may not be	33
of the hiv	33
difference in the	33
with the viral	33
the crystal structure	33
coronavirus associated with	33
hepatitis virus a	33
is similar to	33
spike protein of	33
de groot et	33
surface expression of	33
c for h	33
obtained from dr	33
which is a	33
complete nucleotide sequence	33
was derived from	33
was performed in	33
the fusion peptide	33
of the t	33
subgenomic mrna synthesis	33
the rna was	33
assays were performed	33
zhou et al	33
is necessary for	33
differences between the	33
n pdm infection	33
location of the	33
from that of	33
of the fusion	33
rna and the	33
cells were maintained	33
the membranes were	33
results indicated that	33
in the mhv	33
lysates were prepared	33
subcellular localization of	33
not detected in	33
was resuspended in	33
ibv n protein	33
as in the	33
of this study	33
was present in	33
results demonstrate that	33
been identified in	33
inhibition of the	33
the membrane was	33
japanese encephalitis virus	33
into the cns	33
not affect the	33
the possibility of	33
essential for virus	32
the porcine reproductive	32
was replaced with	32
meulenberg et al	32
mosaic virus rna	32
of these two	32
detection of the	32
the secretory pathway	32
at day p	32
t cell activation	32
a template for	32
infectious cdna clone	32
during the course	32
half of the	32
protein ion channel	32
the nucleotide sequence	32
can be used	32
described in materials	32
studies on the	32
the jhm strain	32
by flow cytometry	32
the significance of	32
possibility that the	32
the mixture was	32
introduced into the	32
of the infectious	32
at hr postinfection	32
viral gene expression	32
that had been	32
for min with	32
the united states	32
to examine the	32
and used for	32
genome sequence of	32
at the time	32
the s and	32
of the family	32
shown in table	32
to investigate whether	32
spaan et al	32
in the central	32
murine leukemia virus	32
to viral infection	32
at least two	32
of sg rna	31
fleming et al	31
of the full	31
of the cellular	31
jhm strain of	31
were washed once	31
expression in the	31
binding to the	31
i and ii	31
to detect the	31
the incorporation of	31
proteins were detected	31
sequences in the	31
may play a	31
of simian virus	31
in patients with	31
are representative of	31
can be found	31
we were able	31
residues of the	31
at and h	31
mediated by the	31
this study was	31
of the infected	31
liao et al	31
carried out in	31
thought to be	31
incubated with the	31
untranslated region of	31
of the severe	31
of virus replication	31
resulting in a	31
u mg cells	31
used to infect	31
were obtained by	31
santa cruz biotechnology	31
h n f	31
by use of	31
of the m	31
a consequence of	31
was amplified by	31
and identification of	31
peste des petits	31
a protein of	31
t cells in	31
compared with the	31
associated with severe	31
reduction in the	31
no effect on	31
studies have shown	31
was due to	31
was cloned into	31
infected with pedv	31
the supernatants were	31
of three independent	31
is the most	31
of the proteins	31
of both the	31
respiratory syncytial virus	31
the differences in	31
binding of the	31
after infection with	31
the nonpermissive temperature	31
of the hemagglutinin	31
experiments were performed	30
human coronavirus oc	30
infected with wild	30
targeted to the	30
not appear to	30
viruses in the	30
and orf b	30
haan et al	30
leading to the	30
in vitro transcripts	30
probably due to	30
infection with the	30
of the immune	30
orf a and	30
with the n	30
in viral rna	30
the involvement of	30
the er and	30
the consensus sequence	30
des petits ruminants	30
not present in	30
n f l	30
of vesicular stomatitis	30
carried out by	30
incubated for hr	30
were transferred to	30
determination of the	30
de haan et	30
not due to	30
of the jhm	30
generation of a	30
the university of	30
biology of coronaviruses	30
that the virus	30
at this time	30
likely that the	30
and h n	30
infected with wt	30
in the genome	30
n protein is	30
room temperature for	30
it would be	30
one or more	30
with an anti	30
that in the	30
the analysis of	30
alveolar type i	30
we have shown	30
and replication of	30
specific t cells	30
k and k	30
required for virus	30
subgenomic mrna transcription	30
at the end	30
is known about	30
at the nonpermissive	30
in transfected cells	30
data indicated that	30
cov s protein	30
d and e	30
transcription initiation site	30
or infected with	30
of the virion	30
b protein is	30
been reported for	30
be associated with	29
in the spike	29
tobacco mosaic virus	29
with a mixture	29
in the c	29
absence of e	29
viruses have been	29
tissue culture cells	29
pcr product was	29
fused to the	29
listed in table	29
were resuspended in	29
results in the	29
evidence for a	29
in terms of	29
the finding that	29
in accordance with	29
of dbt cells	29
of b protein	29
by the presence	29
at days post	29
as a negative	29
we investigated the	29
in the two	29
the time of	29
mice immunized with	29
primary cultures of	29
extracted from the	29
complementary to the	29
the medium was	29
to analyze the	29
released into the	29
when cells were	29
reported that the	29
orf and orf	29
to the nucleolus	29
the processing of	29
virus from the	29
fixed with paraformaldehyde	29
we demonstrated that	29
to understand the	29
we did not	29
used to detect	29
respect to the	29
to test the	29
low levels of	29
effect of the	29
were pretreated with	29
identical to the	29
in the human	29
to bind to	29
these results demonstrate	29
at the n	29
h at rt	29
of dengue virus	29
amino acids in	29
which is the	29
genomic rna and	29
the same as	29
hela cells were	29
substitutions in the	28
that the expression	28
product of the	28
plays a role	28
role in viral	28
has been observed	28
of the di	28
the order nidovirales	28
transcription and translation	28
of feline infectious	28
levels of the	28
within the cns	28
its role in	28
the parental jhm	28
to of the	28
three times in	28
observed in cells	28
but not the	28
gene expression in	28
may contribute to	28
could be due	28
is not required	28
cytoplasmic tail of	28
the cleavage of	28
virus nucleocapsid protein	28
nature of the	28
is a functional	28
from the cell	28
infected cells by	28
were generated by	28
similar results were	28
was mixed with	28
luytjes et al	28
of the mutant	28
and h p	28
rna genome of	28
functions of the	28
fusion activity of	28
the h n	28
the p position	28
at a moi	28
of the disease	28
carried out as	28
integral membrane protein	28
was used in	28
downstream from the	28
the recovery of	28
boiled for min	28
in the formation	28
has been proposed	28
the sequences of	28
were seeded in	28
is an important	28
of murine coronaviruses	28
we observed that	28
with or without	28
e protein ic	28
for the synthesis	28
of di rnas	28
identification and characterization	28
shen et al	28
to be important	28
account for the	28
by the manufacturer	28
were prepared and	28
n protein and	28
entry into the	28
we conclude that	28
were collected at	28
it will be	28
virus titers were	28
only in the	28
the kinetics of	28
of the replication	28
protein has been	28
genbank accession no	28
by in vitro	28
to have a	28
released from the	28
apoptotic cell death	28
structural proteins of	28
after h of	28
a and d	27
length cdna clone	27
and transfected with	27
to be involved	27
at the plasma	27
positive strand rna	27
were confirmed by	27
the amounts of	27
pasternak et al	27
been implicated in	27
titers were determined	27
difference between the	27
min at c	27
synthesis of a	27
the localization of	27
order to determine	27
and it is	27
equine encephalitis virus	27
expressed on the	27
were expressed as	27
both of which	27
analysis revealed that	27
with a single	27
were shown to	27
the northern territory	27
in both the	27
a density of	27
that inhibition of	27
makino et a	27
the same time	27
of the g	27
was measured by	27
and amino acid	27
assay was performed	27
dediego et al	27
a decrease in	27
with h n	27
the functions of	27
which has been	27
of a protein	27
are presented as	27
to the cytoplasm	27
type culture collection	27
and e proteins	27
infection of cells	27
is an enveloped	27
structural and functional	27
wild type virus	27
virus and the	27
the middle east	27
sun et al	27
into the cytoplasm	27
incubated at c	27
surface of the	27
may be important	27
of spike protein	27
cells at h	27
by incubation with	27
baric et al	27
present within the	27
were analyzed for	27
results show that	27
genetics system for	27
cells within the	27
the position of	27
was found that	27
in pbs and	27
are indicated by	27
viral replication in	27
at a concentration	27
was obtained by	27
in vitro transcribed	27
total number of	27
were separated on	27
of the arterivirus	27
of the mrna	27
of interest to	27
mapping of the	27
incorporated into the	27
molecular basis of	27
has been identified	27
ziebuhr et al	27
rna synthesis and	27
infectious virus was	27
in a cell	27
gene expression and	27
terminal amino acids	27
in the second	27
well as in	27
of this protein	26
b and b	26
to wild type	26
ns and ns	26
internal ribosome entry	26
in all cases	26
vennema et al	26
virus entry into	26
type i ifns	26
red clover necrotic	26
receptor for the	26
modification of the	26
gp and gp	26
a functional receptor	26
the initiation of	26
evolution of the	26
by electron microscopy	26
previous studies have	26
were then incubated	26
avian coronavirus infectious	26
also been reported	26
infection with a	26
twice with pbs	26
a previous study	26
clover necrotic mosaic	26
infected with either	26
the hypothesis that	26
a region of	26
ortego et al	26
ph gradient electrophoresis	26
mutant s protein	26
then infected with	26
in this case	26
supernatants were harvested	26
viral rna and	26
correlated with the	26
of the gradient	26
and then incubated	26
this indicates that	26
been identified as	26
to the plasma	26
in balb c	26
carried out using	26
were mixed with	26
type ii cells	26
african green monkey	26
western blot with	26
within the brains	26
in a dose	26
the cell membrane	26
of the envelope	26
a range of	26
regulation of the	26
of the gel	26
influenza virus hemagglutinin	26
to be an	26
yuan et al	26
a critical role	26
to be determined	26
rule out the	26
the sizes of	26
epidermal growth factor	26
meertens et al	26
in human cells	26
site in the	26
by pcr using	26
of the capsid	26
acts as a	26
n protein in	26
is that the	26
the gene encoding	26
for h in	26
copy of the	26
confirm that the	26
followed by the	26
that the two	26
is required to	26
the method of	26
and the cell	26
a negative control	26
the two proteins	26
one of these	26
that the presence	26
essential for viral	26
be able to	26
stages of the	26
functional receptor for	26
collected from the	26
that have been	26
t cells and	26
american type culture	26
the infection of	26
viruses such as	26
the virion envelope	26
length of the	26
lower than that	26
of the amino	26
it was not	25
in cells expressing	25
protein encoded by	25
analyzed by electrophoresis	25
but not with	25
oc e protein	25
shi et al	25
the coronavirus mouse	25
cd c cd	25
phylogenetic analysis of	25
a highly conserved	25
result in the	25
subjected to immunoprecipitation	25
were fixed in	25
that both the	25
some of these	25
negative strand rna	25
ye et al	25
in the lung	25
atllf cord virus	25
cloning of the	25
to form a	25
the s proteins	25
that the s	25
is one of	25
the large di	25
in the virion	25
of monoclonal antibodies	25
transfected with plasmids	25
a moi of	25
levels of viral	25
confluent monolayers of	25
kuo et al	25
mice at day	25
wilde et al	25
of infection of	25
see materials and	25
cdna clones were	25
in the expression	25
the virus to	25
transported to the	25
g to s	25
recent studies have	25
acids of the	25
at the c	25
a portion of	25
and treated with	25
are needed to	25
cells using the	25
of this virus	25
the open reading	25
the current study	25
cell surface expression	25
the immune system	25
large number of	25
viral life cycle	25
removed and the	25
cells were plated	25
expressed in the	25
virus infection of	25
plays an important	25
to define the	25
but not by	25
the virus in	25
on the right	25
of viral infection	25
based on a	25
were cultured in	25
organization of the	25
was required for	25
c virus infection	25
to s phase	25
determine if the	25
of influenza c	25
novel coronavirus associated	25
as early as	25
it should be	25
subunit of the	25
clarified by centrifugation	25
at least one	25
cells with the	25
rous sarcoma virus	25
to the n	25
the first time	25
and centrifuged at	25
that the n	25
of the structural	25
the distribution of	25
to generate a	25
innate and adaptive	25
we were unable	25
transcription of the	25
following mhv infection	25
the capsid protein	25
agreement with the	25
cov spike protein	25
we compared the	25
bound to the	25
cells of the	25
in the respiratory	25
of the most	25
at hr p	25
patients with severe	25
of the central	25
sg rna synthesis	25
a group of	25
in these cells	25
the t promoter	24
amount of the	24
at a density	24
these results are	24
and ns b	24
sequence in the	24
the plates were	24
was performed at	24
expressed from the	24
not result in	24
labeled with s	24
the transcription initiation	24
in the murine	24
is based on	24
of porcine circovirus	24
at least in	24
that expression of	24
of the four	24
that did not	24
complete genome sequence	24
article can be	24
were prepared by	24
of the ifn	24
blot analysis using	24
of southern bean	24
of neutralizing antibodies	24
viral genomic rna	24
prior to infection	24
the treatment of	24
to the viral	24
incubated with a	24
produced by the	24
adaptive immune responses	24
of the helicase	24
nucleotides in length	24
to this end	24
strains of mhv	24
proteins have been	24
balb cv tumor	24
are essential for	24
was associated with	24
deng et al	24
of the replicase	24
pbs for min	24
seen in the	24
products were analyzed	24
were determined using	24
the coat protein	24
and analysis of	24
the potential of	24
of the purified	24
viral rna in	24
c for s	24
cd c cells	24
viral protein expression	24
de wilde et	24
in the legend	24
in some cases	24
hcv core protein	24
the spinal cord	24
it is conceivable	24
and processing of	24
an in vitro	24
the polymerase gene	24
the type i	24
our understanding of	24
other viral proteins	24
virus e protein	24
of the large	24
in a previous	24
equal volume of	24
morizono and chen	24
insights into the	24
of the innate	24
is believed to	24
important for the	24
of vaccinia virus	24
for min to	24
were visualized by	24
hu et al	24
of mice infected	24
stability of the	24
the rv g	24
nucleocapsid protein of	24
we analyzed the	24
it is unlikely	24
potato virus x	24
in comparison to	24
in hela cells	24
to note that	24
to replicate in	24
the nature of	24
at different time	24
it is interesting	24
infected cells with	24
sodium dodecyl sulfate	24
for s and	24
stohlman et al	24
of lipid rafts	24
for the expression	24
petits ruminants virus	24
this article can	24
h prior to	24
fractions were collected	24
sequence of a	24
the viral replicase	24
supported by a	24
in dbt cells	24
been detected in	24
identified in the	24
the transmissible gastroenteritis	24
in the united	24
syndrome coronavirus spike	24
defective interfering rna	24
importance of the	24
form of the	24
at h after	24
of the in	24
coronavirus envelope protein	24
been observed in	24
of the ibv	24
protein is the	24
had no effect	24
for in vitro	24
a strain of	24
is due to	24
remain to be	24
cells as described	24
culture supernatants were	24
with endo h	24
ibv e and	24
on vero cells	24
is composed of	24
the pcr product	24
and the c	23
the entire genome	23
isolation of a	23
the impact of	23
has been previously	23
is dispensable for	23
bacteriophage t rna	23
from the nucleus	23
zhao et al	23
the hepatitis c	23
cells were stained	23
the roles of	23
evidence that the	23
production of infectious	23
in the brains	23
protein that is	23
a panel of	23
the lungs of	23
replication in cell	23
proteins from the	23
cd t lymphocytes	23
removed from the	23
on ice for	23
were derived from	23
little is known	23
minimal essential medium	23
study of the	23
which had been	23
effects of the	23
an equal volume	23
tumor necrosis factor	23
positions of the	23
tgbp and tgbp	23
an increase in	23
plaque assay on	23
the p mapk	23
the amino acids	23
the isolation of	23
small amount of	23
the entry of	23
coronavirus s protein	23
necrotic mosaic virus	23
the pathogenicity of	23
to form the	23
that the protein	23
protein was also	23
association of the	23
bind to the	23
to infection with	23
amino acids to	23
were centrifuged at	23
antibodies specific for	23
belongs to the	23
inhibitory effect of	23
been associated with	23
infectivity of the	23
and the s	23
expression of viral	23
and days post	23
cells in vitro	23
is conceivable that	23
of peptide h	23
the cell lysates	23
subgenomic rna synthesis	23
not in the	23
functional analysis of	23
also been shown	23
been suggested that	23
alignment of the	23
of cells with	23
molecular mass of	23
deletion of the	23
activation of nf	23
with the recombinant	23
gene in the	23
cell lines and	23
different time points	23
genomic and subgenomic	23
n a icv	23
results were obtained	23
correspond to the	23
we demonstrate that	23
and precipitated with	23
of the peplomer	23
of the total	23
a sucrose cushion	23
cells and in	23
we also thank	23
which contains the	23
to the same	23
belong to the	23
multiple sequence alignment	23
followed by cycles	23
thermo fisher scientific	23
and the resulting	23
been reported that	23
the top of	23
interferon regulatory factor	23
be responsible for	23
the parental virus	23
in the supernatant	23
in our study	23
sites in the	23
in part by	23
an integral membrane	23
the expected size	23
following infection with	23
consistent with our	23
of bovine coronavirus	23
and viral rna	23
identified as a	23
medium supplemented with	23
and function of	23
lucas et al	23
for the replication	23
of the small	23
in the pathogenesis	23
of t cell	23
in each of	23
adjacent to the	23
ice for min	23
of middle east	23
the origin of	23
spike protein in	23
of p and	23
the deduced amino	23
the atpase activity	23
low level of	23
is a common	23
used to generate	23
illustrated in fig	23
out the possibility	23
of the glycoprotein	23
c cd b	23
collected by centrifugation	23
the emergence of	23
of the brain	23
included in the	23
a mouse model	23
possibility is that	23
isolation and characterization	23
associated with this	23
to a final	22
were lysed in	22
may also be	22
cells were pretreated	22
coronavirus spike proteins	22
laemmli sample buffer	22
amino acid identity	22
dveksler et al	22
at the permissive	22
the viral nucleocapsid	22
reading frame of	22
by using the	22
reported to be	22
detected in cells	22
h after infection	22
identification of two	22
due to its	22
sd and sk	22
considered to be	22
used to determine	22
in vivo and	22
may have been	22
the brain and	22
rna polymerase promoter	22
be essential for	22
extracted with phenol	22
venezuelan equine encephalitis	22
in any of	22
inoculum was removed	22
the pellet was	22
titer was determined	22
coronavirus rna synthesis	22
the t rna	22
of the coronaviridae	22
host innate immune	22
with the exception	22
mutants of mouse	22
on the virion	22
during viral infection	22
at for hr	22
leader sequence of	22
the cavv cl	22
separated by sds	22
and infected with	22
a murine coronavirus	22
molecular weights of	22
mcbride et al	22
in a beckman	22
may have a	22
wilson et al	22
green monkey kidney	22
and used as	22
are expressed as	22
for reprints should	22
of m protein	22
expressed as the	22
protein was not	22
the culture medium	22
was calculated as	22
a set of	22
the inhibitory effect	22
the data presented	22
well plates were	22
decrease in the	22
demonstrating that the	22
the reduction of	22
well plates and	22
marra et al	22
sequences flanking the	22
and activation of	22
which have been	22
and used to	22
the envelope glycoprotein	22
for viral rna	22
for the development	22
was amplified from	22
was examined by	22
a reduction in	22
of other viral	22
requests for reprints	22
in the spleen	22
infection in vitro	22
derived from a	22
hofmann et al	22
we have used	22
viral and cellular	22
and did not	22
to speculate that	22
were removed by	22
the heptad repeat	22
were amplified by	22
in the sequence	22
the virus is	22
the cell cycle	22
geisbert et al	22
fischer et al	22
and evolution of	22
grants from the	22
in coronavirus assembly	22
wong et al	22
of overlapping genes	22
intracellular rna was	22
at and hpi	22
can be detected	22
more than one	22
of the corresponding	22
was verified by	22
the di rnas	22
are present in	22
activated protein kinase	22
protein interacts with	22
of the gp	22
nucleocapsid protein and	22
were washed and	22
depends on the	22
when compared with	22
was identified in	22
rest of the	22
the translation of	22
rabbit reticulocyte lysate	22
the product of	22
t cells are	22
causative agent of	22
to play a	22
on viral replication	22
from the same	22
inhibition of erk	22
system for the	22
a laboratory manual	22
brome mosaic virus	22
it is known	22
antibodies to the	22
were expressed in	22
in mice and	22
and western blot	22
at and days	22
a model for	22
and adaptive immune	21
early in infection	21
that can be	21
of viral protein	21
transferred to nitrocellulose	21
m and e	21
by the viral	21
of a virus	21
infected or infected	21
structure and function	21
been previously described	21
the national institutes	21
strain of mouse	21
a large number	21
the occurrence of	21
the range of	21
performed as previously	21
van dinten et	21
length infectious cdna	21
the degree of	21
nucleotide sequences of	21
influenza virus infection	21
and functional characterization	21
protein is an	21
of virus infection	21
this could be	21
s protein in	21
are thought to	21
multisystemic wasting syndrome	21
was detected with	21
dinten et al	21
change in the	21
hingley et al	21
porcine circovirus type	21
interactions between the	21
the coding region	21
also present in	21
for analysis of	21
in the liver	21
of bamboo mosaic	21
for the detection	21
in the culture	21
of coronavirus rna	21
the cytoplasm and	21
of the hr	21
formation of a	21
a dilution of	21
the a strain	21
with those of	21
and the virus	21
caused by the	21
of rna viruses	21
it was also	21
in a similar	21
yu et al	21
at day post	21
involved in virus	21
explained by the	21
to each other	21
the causative agent	21
were detected with	21
was determined using	21
cell culture and	21
did not show	21
the rest of	21
carried out with	21
the intergenic region	21
excellent technical assistance	21
early stages of	21
by grants from	21
m and m	21
supplementary table s	21
wistar furth rats	21
stages of infection	21
interaction of the	21
the results obtained	21
h and then	21
c virus replication	21
be explained by	21
case of the	21
the process of	21
obtained with the	21
protein with a	21
the host immune	21
the notion that	21
been reported in	21
of nsp and	21
mhv infection of	21
of actinomycin d	21
bosch et al	21
higher levels of	21
at day pi	21
the mhv s	21
coronavirus infection in	21
a determinant of	21
binding domain of	21
of viral particles	21
ccl and ccl	21
were fixed and	21
other members of	21
believed to be	21
origin of the	21
cov n protein	21
reduction of the	21
detected with a	21
out of the	21
vitro transcription and	21
studies suggest that	21
a and a	21
at g for	21
to the sgrna	21
was treated with	21
evidence suggests that	21
incubated at for	21
african horse sickness	21
reprints should be	21
cell responses in	21
to whom requests	21
the center of	21
high degree of	21
use of a	21
coding region of	21
the infectious bronchitis	21
was observed for	21
was grown in	21
the family coronaviridae	21
of the viruses	21
directed against the	21
replication of rna	21
the association of	21
syndrome coronavirus infection	21
rna species of	21
functional characterization of	21
disruption of the	21
n and h	21
was purchased from	21
aspects of the	21
proteins b and	21
in the number	21
for h before	21
supported by grants	21
at days and	21
s proteins of	21
a subset of	21
e glycoprotein of	21
in escherichia coli	21
model for the	21
protein expression was	21
than those of	21
consistent with a	21
a component of	21
domains of the	21
whom requests for	21
degradation of the	21
the molecular basis	21
infected with prrsv	21
is a major	21
the reaction was	21
in orf b	21
with the corresponding	21
samples were incubated	21
were similar to	21
the mechanism by	21
the rub genome	21
rna replication and	21
as templates for	21
r n a	21
c virus rna	21
is incorporated into	21
yellow mosaic virus	21
have been isolated	20
we could not	20
between these two	20
conformation of the	20
yokomori et al	20
acid substitutions in	20
of the h	20
are likely to	20
has been studied	20
major histocompatibility complex	20
treatment of the	20
sanchez et al	20
willey et al	20
many of the	20
infection and the	20
to compare the	20
and then infected	20
genome of the	20
for subgenomic mrna	20
its ability to	20
molecular weight markers	20
bnip gene expression	20
at the p	20
roles in the	20
p mapk pathway	20
with fetal calf	20
were infected at	20
e protein and	20
linked to the	20
titers in the	20
viral titers were	20
have been detected	20
murine coronavirus mouse	20
triple gene block	20
been found to	20
in the amount	20
of the b	20
is also possible	20
to serve as	20
in mice infected	20
and the rna	20
deletions in the	20
known about the	20
proteins encoded by	20
of the infection	20
predicted to be	20
function as a	20
subjected to western	20
the properties of	20
harvested at h	20
cells that were	20
with the s	20
addition of the	20
the rate of	20
mice were infected	20
virus nonstructural protein	20
in the rna	20
confirmed that the	20
to the virus	20
could be used	20
ranging from to	20
transfected with a	20
incubated in the	20
amino acid changes	20
the complete sequence	20
also possible that	20
the ts mutant	20
with monoclonal antibodies	20
lavi et al	20
receptor binding domain	20
and found that	20
consistent with this	20
on the presence	20
the results are	20
the lipid rafts	20
enjuanes et al	20
is important in	20
could also be	20
has shown that	20
would be expected	20
to ensure that	20
able to replicate	20
the idea that	20
as the template	20
in these experiments	20
nelsen et al	20
infected cell lysates	20
was generated by	20
rather than the	20
were propagated in	20
was observed with	20
of cells expressing	20
respiratory tract of	20
the length of	20
and with the	20
serve as a	20
red blood cells	20
in the family	20
the first and	20
each sample was	20
the he gene	20
for interaction with	20
the influence of	20
was removed by	20
plus strand rna	20
of hepatitis b	20
role in virus	20
reverse genetics systems	20
the largest rna	20
leads to the	20
virus production was	20
t dna polymerase	20
the expression levels	20
groot et al	20
for the viral	20
equal amounts of	20
with mouse hepatitis	20
the spread of	20
yet to be	20
to the parental	20
in the early	20
to virus infection	20
structures of the	20
cellular and viral	20
replication and transcription	20
the phosphorylation of	20
are indicated in	20
the total number	20
a and orf	20
mhv s protein	20
and inserted into	20
in a single	20
bgp b ntd	20
accordance with the	20
nested set of	20
medium was replaced	20
the spike gene	20
affected by the	20
important roles in	20
forms of the	20
the sensitivity of	20
for the generation	20
of subgenomic mrnas	20
shown in the	20
this is consistent	20
was subjected to	20
located within the	20
amounts of the	20
data demonstrate that	20
in complex with	20
were identified in	20
can also be	20
cells were used	20
sawicki and sawicki	20
is not yet	20
to reduce the	20
in orf a	20
products of the	20
miller et al	20
the reduction in	20
of structural proteins	20
a amino acid	20
the beginning of	20
the vaccinia virus	20
in the host	20
basis for the	20
virus characterization of	20
amino acids and	20
incubated at the	20
the viral life	20
was also observed	20
a family of	20
purified as described	20
of atp b	20
mice were immunized	20
in blocking buffer	20
of a human	20
novel coronavirus in	20
performed using a	20
are required to	20
from the first	20
regulation of bnip	20
of gene expression	20
of the protease	19
as has been	19
proteins that are	19
as a possible	19
are responsible for	19
protein may be	19
virus entry and	19
may be involved	19
to infect cells	19
in the online	19
in insect cells	19
were isolated and	19
required for viral	19
were prepared from	19
as part of	19
genomic rna is	19
were positive for	19
in the golgi	19
from cells infected	19
paraformaldehyde for min	19
barley stripe mosaic	19
were lysed with	19
of p in	19
of coronavirus mhv	19
s gene of	19
findings suggest that	19
of plasmid dna	19
cv tumor cells	19
in the current	19
amplified by rt	19
and can be	19
min at rt	19
we report the	19
to test whether	19
is most likely	19
to be required	19
is the only	19
top of the	19
of the deletion	19
and release of	19
two of the	19
used in these	19