trigram

This is a table of type trigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

trigram	frequency
the presence of	72
in this study	72
of the virus	58
analysis of the	55
according to the	53
amino acid sequences	52
h n viruses	48
as well as	47
based on the	47
amino acid sequence	45
of the s	44
porcine epidemic diarrhea	42
the s glycoprotein	40
virus genes doi	40
hpai h n	38
a g t	34
the s subunit	33
t t a	33
deduced amino acid	33
gray and jmk	32
g t t	32
codon usage bias	32
b e a	32
epidemic diarrhea virus	32
j m k	32
all of the	32
c a t	31
the gray and	30
of h n	30
a c a	30
and jmk strains	30
the s gene	29
sequence of the	29
transmissible gastroenteritis virus	29
was used to	28
the n gene	28
c t a	28
a g c	28
t a g	28
end of the	28
in order to	27
the amino acid	27
a a g	27
a c t	26
t c a	26
a t a	26
sequences of the	26
a t t	25
t t t	25
t g a	25
c a c	25
t t g	25
closely related to	24
t a t	24
the leader rna	24
t a a	24
region of the	24
of the viral	24
a c c	24
a g a	24
the nucleotide sequence	24
a a a	24
of the genome	23
c a a	23
amino acid changes	23
g a g	23
a a c	23
data not shown	21
g c t	21
t a c	21
c c a	21
was carried out	21
a t g	21
due to the	21
phylogenetic analysis of	21
a t c	21
the nucleotide sequences	21
the m gene	21
infectious bronchitis virus	21
c a g	21
downstream of the	21
t g g	21
the codon usage	20
m k b	20
the detection of	20
one of the	20
of porcine epidemic	20
t g t	20
nucleotide sequences of	20
and amino acid	20
a total of	20
showed that the	20
k b e	20
found to be	19
the s and	19
shown in fig	19
t c t	19
g c a	19
acid sequences of	19
upstream of the	19
of the rt	19
found in the	19
amino acid differences	18
g t a	18
h n virus	18
it has been	18
g a c	18
tgev and prcv	18
the n protein	18
nucleotide sequence of	18
c t g	18
members of the	18
deletion in the	18
were found to	17
was used for	17
c g a	17
the deduced amino	17
part of the	17
c t c	17
of the n	17
the entire genome	17
the s protein	17
g a a	17
sequence analysis of	17
as shown in	16
g g c	16
g t c	16
a role in	16
rna was extracted	16
the amount of	16
a c g	16
open reading frame	16
n gene of	16
used as a	16
associated with the	16
amino acid substitutions	16
was used as	16
dependent rna polymerase	16
as previously described	16
to be the	16
orf a and	16
the sequence of	15
c t t	15
g a t	15
of the viruses	15
leader rna sequences	15
the majority of	15
role in the	15
to determine the	15
the viral rna	15
highly pathogenic avian	15
was performed using	15
pathogenic avian influenza	15
of the pedv	15
the present study	15
type ii fcov	15
the sequences of	15
cv and br	15
g t g	15
was extracted from	15
a a t	15
observed in the	15
each of the	15
avian influenza a	15
a number of	15
the end of	15
avian influenza viruses	14
and d br	14
of the leader	14
involved in the	14
in the s	14
porcine transmissible gastroenteritis	14
nucleotide and amino	14
the online version	14
g g t	14
cl and d	14
g g a	14
which is available	14
a g g	14
most of the	14
pathogenic and attenuated	14
synonymous codon usage	14
in cell culture	14
t c g	14
for the s	14
online version of	14
pcr products were	14
the number of	14
of the same	14
avian influenza virus	14
of avian influenza	14
comparison of the	14
indicated that the	14
acid sequence of	14
play a role	13
contains supplementary material	13
were collected from	13
s and s	13
viral rna was	13
t t c	13
t g c	13
open reading frames	13
version of this	13
available to authorized	13
of amino acid	13
of this article	13
p and p	13
to authorized users	13
the complete genome	13
resulted in the	13
was detected in	13
that of the	13
of the two	13
for h at	13
the m protein	13
is available to	13
belong to the	13
the results of	13
for min at	13
in contrast to	12
differences in the	12
present in the	12
c g t	12
in addition to	12
gene of the	12
with that of	12
related to the	12
changes in the	12
lsu and ok	12
to investigate the	12
a member of	12
were used for	12
of the h	12
the formation of	12
the viral genome	12
case fatality rate	12
was found to	12
in the present	12
the pcr products	12
protein is a	12
in the field	12
codon usage in	12
similar to the	12
indicating that the	12
as described previously	12
member of the	12
h n influenza	12
shown to be	12
used in this	12
to that of	12
located in the	12
amino acid residues	12
for the detection	11
in the usa	11
been shown to	11
acid sequences were	11
at the end	11
and orf b	11
of these viruses	11
e a c	11
e a t	11
the h protein	11
effective codon number	11
isolated from the	11
regions of the	11
of orf a	11
of the gray	11
in our study	11
shown in table	11
were obtained from	11
was supported by	11
cells transfected with	11
the cells were	11
used for the	11
responsible for the	11
g c g	11
the f protein	11
the chinju n	11
usage bias in	11
methyl transferase activity	11
detection limit of	11
gc content of	11
structure of the	11
well as the	11
samples collected from	11
electronic supplementary material	11
the development of	11
m and n	11
between the s	11
has been shown	11
and n genes	11
of the virion	10
is the first	10
a and orf	10
s indel pedv	10
carried out using	10
to those of	10
s e i	10
c c t	10
strains of ibv	10
of codon usage	10
to study the	10
this work was	10
polymerase chain reaction	10
results showed that	10
different from the	10
for the presence	10
severe acute respiratory	10
of the m	10
influenza a virus	10
of influenza a	10
consistent with the	10
was isolated from	10
samples were collected	10
chicken and turkey	10
acute respiratory syndrome	10
leader rna sequence	10
the rd position	10
is an important	10
and phylogenetic analysis	10
this is the	10
extracted from the	10
influenza a viruses	10
the effective codon	10
to be a	10
of cdv strains	10
nucleotides downstream of	10
of cv and	10
for min and	10
were used to	10
of the disease	10
be involved in	10
was added to	10
and attenuated viruses	9
was observed in	9
complete genome sequences	9
of the tgev	9
the h gene	9
a final extension	9
gene of pedv	9
be due to	9
in south korea	9
been used to	9
except for the	9
presence of a	9
the analysis of	9
rpv l protein	9
of a novel	9
can be used	9
followed by a	9
the evolution of	9
the extent of	9
genetic diversity of	9
a h n	9
compared with the	9
of hpai h	9
usage and dinucleotide	9
derived from the	9
detected in the	9
with the exception	9
of the sequences	9
any of the	9
the genomic rna	9
of tgev and	9
a j m	9
the fact that	9
a and b	9
the virus in	9
the results showed	9
a variety of	9
were observed in	9
those of other	9
porcine epidemic diarrhoea	9
sequences were aligned	9
to amplify the	9
analyses of the	9
codon usage variation	9
type i fcov	9
the generation of	9
the m and	9
has also been	9
appeared to be	9
are shown in	9
primers were designed	9
were inoculated with	9
jmk strains had	9
differences between the	9
the exception of	9
genome sequences of	9
in st cells	9
of pedv in	9
this study was	9
acid deletion in	8
in which the	8
content of the	8
as described above	8
and the other	8
is the main	8
g j m	8
size of the	8
thought to be	8
performed using the	8
n avian influenza	8
was identified in	8
of the nucleotide	8
of the first	8
chinju n gene	8
genome consists of	8
analysis indicated that	8
veroe cells were	8
when compared to	8
c c c	8
there is a	8
the distance between	8
were also found	8
h n avian	8
the signal peptide	8
other members of	8
have shown that	8
characteristics of the	8
to compare the	8
amino acid identity	8
followed by cycles	8
t c c	8
of the vt	8
the first report	8
genbank accession numbers	8
a and ab	8
m and pgenesil	8
were used in	8
to each other	8
gene of tgev	8
appear to be	8
correlation between the	8
the nucleotide and	8
transfected with prnat	8
molecular detection of	8
in the presence	8
of the codon	8
m gene of	8
of the spike	8
sour orange seedlings	8
in the spike	8
to better understand	8
pedv n gene	8
codon usage and	8
have been detected	8
high case fatality	8
the production of	8
the gc content	8
casein kinase ii	8
the prevalence of	8
specific for the	8
between residues and	8
the secondary structure	8
in mammalian cells	8
detection of pedv	8
and stored at	8
characterization of the	8
sequences were analyzed	8
s subunit of	8
was propagated in	8
base usage and	8
genome sequence of	8
constructed using the	8
min at c	8
of the partial	8
compared to the	8
c g g	8
vp and vp	8
also found in	8
the sensitivity of	8
portion of the	8
targeting the m	8
different strains of	8
the virus was	8
and deduced amino	8
is shown in	8
there was no	8
agarose gel electrophoresis	8
were analyzed by	8
supported by the	8
of tgev in	8
been detected in	8
of the host	8
the ga viruses	7
the replication of	7
ll of the	7
tree was constructed	7
were used as	7
the receptor binding	7
the hypervariable region	7
n protein of	7
tgev tmk strain	7
we found that	7
signal peptide region	7
major structural proteins	7
the international committee	7
an important role	7
the beginning of	7
between the two	7
in the n	7
leading to the	7
base usage at	7
could not be	7
the ark and	7
and respiratory syndrome	7
is a major	7
transient expression of	7
the rt reaction	7
of the cdv	7
in the case	7
in this paper	7
with those of	7
mediated isothermal amplification	7
as can be	7
for the rt	7
some of the	7
showed the presence	7
to determine whether	7
located within the	7
important role in	7
coding for the	7
type ii fcovs	7
to identify the	7
international committee on	7
designed based on	7
specific monoclonal antibodies	7
by cycles of	7
amino acid deletion	7
strain was isolated	7
the occurrence of	7
more closely related	7
the amino acids	7
work was supported	7
epidemic diarrhoea virus	7
period of the	7
of the international	7
for detection of	7
of porcine deltacoronavirus	7
used in the	7
position of the	7
chk and trk	7
the absence of	7
is involved in	7
were designed based	7
protein kinase c	7
g c c	7
in codon usage	7
carried out in	7
pedv in the	7
of the rna	7
plays an important	7
the ark viruses	7
new england biolabs	7
n influenza virus	7
e and tgev	7
in the same	7
cave myotis adv	7
bias in csfv	7
the pl protease	7
is specific for	7
of the poxviridae	7
on the viral	7
included in the	7
acid substitutions in	7
the case of	7
of sour orange	7
suggests that the	7
is consistent with	7
was determined by	7
understanding of the	7
of p and	7
of the ibdv	7
to analyze the	7
correlated with the	7
reproductive and respiratory	7
have been identified	7
the porcine epidemic	7
of type ii	7
revealed that the	7
were included in	7
the codon bias	7
were found in	7
receptor binding site	7
and dinucleotide frequency	7
porcine reproductive and	7
alignment of the	7
not detected in	7
of the gene	7
it is not	7
of all amino	7
cryptic splice sites	7
leader complex binding	7
the base usage	7
the leader sequence	7
substitutions in the	7
the use of	7
of the coronavirus	7
sequences in the	7
avian infectious bronchitis	7
of the entire	7
of this study	7
published sequences of	7
could be a	7
aligned with the	7
mouse hepatitis virus	7
analysis revealed that	7
and characterization of	7
of the genus	7
been found to	7
sequence identity with	7
taxonomy of viruses	7
are needed to	7
b and c	7
predicted amino acid	7
short hairpin rnas	7
respiratory syndrome virus	7
the size of	7
a previous study	7
feline infectious peritonitis	7
the ability to	7
the result of	7
all amino acid	7
in comparison with	7
in embryonated eggs	7
results indicated that	7
deposited in genbank	7
into the asia	6
amount of viral	6
of monoclonal antibodies	6
suggest that the	6
is responsible for	6
acid changes between	6
specificity of the	6
the differences in	6
total rna was	6
an amino acid	6
sequences of all	6
strain of tgev	6
in agreement with	6
within the s	6
belonging to the	6
the severity of	6
be used as	6
of tgev infection	6
other bat pyvs	6
and subjected to	6
taq dna polymerase	6
lamp assay was	6
the relationship between	6
c g c	6
for codon usage	6
based on a	6
to have a	6
remains to be	6
the isolation of	6
of the amino	6
in all of	6
each codon positions	6
the host cell	6
have been developed	6
of the predicted	6
sequence for the	6
were detected by	6
of highly pathogenic	6
a bank vole	6
complex binding site	6
needs to be	6
the two strains	6
between type i	6
of l protein	6
t ppi t	6
the same serotype	6
more sensitive than	6
the pathogenic and	6
st cells were	6
at the same	6
species of bats	6
the two viruses	6
the most abundant	6
complementary to the	6
n viruses from	6
of the infected	6
the pedv orf	6
human h n	6
in the genbank	6
identified in the	6
a template for	6
the detection limit	6
positive samples were	6
may be involved	6
be used to	6
genetic and antigenic	6
corresponding to the	6
the existence of	6
orf b is	6
cloned into the	6
were not detected	6
in the natural	6
final extension at	6
component of the	6
the basis of	6
was first reported	6
seen from fig	6
were infected with	6
have been reported	6
of the complete	6
the samples were	6
amino acid composition	6
viruses of the	6
of amino acids	6
analysis was performed	6
the computer program	6
was performed by	6
which is a	6
and has been	6
that codon usage	6
the predicted secondary	6
were aligned using	6
changes between the	6
between the ark	6
with respect to	6
as a template	6
of the ibv	6
of the other	6
in japanese bats	6
in the rd	6
in terms of	6
is not known	6
g g g	6
the spike gene	6
complete genome sequence	6
of the puuv	6
between pathogenic and	6
found that the	6
has not been	6
terminal domain of	6
first reported in	6
located at the	6
were performed using	6
one amino acid	6
the first two	6
p and f	6
the vt strain	6
did not show	6
been reported in	6
the loss of	6
was also found	6
it appears that	6
the genome of	6
acute infectious diarrhoea	6
sensitivity of the	6
results suggest that	6
of chk and	6
was similar to	6
of the nucleoprotein	6
was found in	6
the virus and	6
of genetic mutations	6
plants infected with	6
rna polymerase gene	6
cells infected with	6
the frameshift site	6
for the n	6
and ark dpi	6
the pedv n	6
in any of	6
and s subunits	6
than that of	6
are closely related	6
and the presence	6
of the genomic	6
amino acid motifs	6
the appearance of	6
majority of the	6
classified into the	6
similar to that	6
at each codon	6
and e genes	6
colloidal gold test	6
viruses isolated from	6
is the most	6
the vp gene	6
of the orf	6
n protein is	6
influenza a h	6
world health organization	6
appears to be	6
resulted in a	6
the percentage of	6
formation of a	6
the pathogenicity of	6
in accordance with	6
sequencing of the	6
this study were	6
observed between the	6
is associated with	6
for virus isolation	6
added to the	6
pdcov replication in	6
in the f	6
at the beginning	6
results indicate that	6
m k c	6
the united states	6
and turkey samples	6
porcine respiratory coronavirus	6
of the deduced	6
amount of virus	6
it will be	6
the importance of	6
the mass viruses	6
and was used	6
phylogenetic trees were	6
was constructed using	6
protein of cdv	6
contrast to the	6
which is the	6
of rpv l	6
isolation of a	6
cell culture adapted	6
linked phosphorylation sites	6
of the porcine	5
phylogenetic relationship of	5
the virus to	5
at room temperature	5
and mass viruses	5
into two groups	5
was reduced by	5
were added to	5
gene of porcine	5
the pathogenic virus	5
products of the	5
to examine the	5
from the gel	5
the role of	5
in the amino	5
viral strain was	5
analysis of porcine	5
of the newly	5
genbank accession number	5
the ability of	5
isolated from a	5
in line with	5
complete genome of	5
base usage of	5
two amino acid	5
with other pedv	5
the spike glycoprotein	5
molecular characterization of	5
depending on the	5
key role in	5
of viral rnas	5
is caused by	5
this study are	5
swabs collected from	5
a m c	5
a wide range	5
virus isolation and	5
many cases of	5
m c a	5
identified in a	5
genome of the	5
of the u	5
in a co	5
african green monkey	5
presence of the	5
mammalian expression vectors	5
influenza viruses in	5
located between the	5
sl and s	5
obtained from the	5
relative abundance of	5
the natural host	5
the od value	5
of the genes	5
and sequence analysis	5
first report of	5
relationships between the	5
virus was propagated	5
tmk and ptmk	5
viruses from indonesia	5
results of the	5
reported that the	5
the possibility that	5
korean pedv isolates	5
n gene was	5
the molecular detection	5
t g j	5
nat acad sci	5
for the development	5
end of orf	5
the consensus sequence	5
the bank vole	5
give rise to	5
phosphorylation sites by	5
jmk strains of	5
of the korean	5
and avirulent strains	5
have been found	5
remained to be	5
are listed in	5
that the s	5
fipv orf b	5
acid changes in	5
the diversity of	5
protein of amino	5
around the world	5
late period of	5
of other coronaviruses	5
the expression of	5
it is interesting	5
the cl and	5
used to develop	5
cases of acute	5
were positive for	5
codon usage is	5
predicted secondary structure	5
gc in the	5
referred to as	5
the fecal samples	5
a key role	5
overall gc content	5
not seem to	5
rbcv and ebcv	5
purified using the	5
submitted to genbank	5
the published sequences	5
the genes encoding	5
allantoic fluid was	5
of the hemagglutinin	5
most closely related	5
studies have shown	5
genes of pdcov	5
infectious bursal disease	5
secondary structure predictions	5
human hpai h	5
on taxonomy of	5
n protein had	5
the phylogenetic relationship	5
described in this	5
proc nat acad	5
acknowledgments this work	5
the virus is	5
for the ark	5
litter samples collected	5
on the s	5
single amino acid	5
phylogenetic tree was	5
samples of the	5
the onderstepoort strain	5
the gray strain	5
different from those	5
of the novel	5
classical swine fever	5
under isothermal conditions	5
has been used	5
be interesting to	5
genes encoding the	5
were calculated by	5
out in a	5
belongs to the	5
in our laboratory	5
the cleavage site	5
virulent and avirulent	5
with regard to	5
of the total	5
in the region	5
can be the	5
nucleotide and deduced	5
the similarity of	5
is necessary to	5
have been described	5
trees were constructed	5
nucleotide sequences were	5
to detect pedv	5
c b e	5
with the published	5
on the other	5
to our knowledge	5
by reverse transcription	5
for each virus	5
in coa and	5
in this respect	5
sequence was determined	5
was calculated for	5
is interesting to	5
strains of the	5
is a member	5
the effect of	5
inhibit pdcov replication	5
kidney cell line	5
avian h n	5
and ll of	5
from alemow plants	5
gold test strip	5
wide range of	5
virus in the	5
to the viral	5
of transmissible gastroenteritis	5
of the three	5
the kda orf	5
of puuv isolates	5
because of the	5
human cases of	5
the study of	5
with a large	5
n viruses in	5
are known to	5
in the united	5
region in the	5
type i fcovs	5
the sequences were	5
are found in	5
the virulence of	5
committee on taxonomy	5
from insect cells	5
rna from the	5
the length of	5
the structural proteins	5
of codon bias	5
found to have	5
based elisa was	5
us to examine	5
the variation in	5
high mutation rate	5
was found that	5
the binding of	5
cells were infected	5
at h post	5
s glycoprotein gene	5
it is possible	5
was higher than	5
of the dinucleotide	5
usage in csfv	5
connecticut and florida	5
from the original	5
of pedv and	5
better understand the	5
sequence of pedv	5
report of the	5
diseases of swine	5
the h n	5
f protein of	5
chinju n protein	5
higher than the	5
of the outbreak	5
ark and ark	5
for the rdrp	5
may explain the	5
than the expected	5
the relative abundance	5
listed in table	5
may play a	5
structural gene region	5
the nucleoprotein gene	5
consists of a	5
the orf a	5
the plates were	5
reported to have	5
the synthesis of	5
it was found	5
primers for the	5
genomic gc content	5
s and sm	5
of all the	5
was based on	5
reaction mixture was	5
detection of novel	5
cells were incubated	5
amber stop codon	5
most similar to	5
on the sequence	5
clinical signs were	5
infectious diarrhoea in	5
m t g	5
the family coronaviridae	5
the genomes of	5
is thought to	5
nucleotide sequence data	5
in ll of	5
chickens and turkeys	5
to detect the	5
the attenuated virus	5
were obtained by	5
led us to	5
the sy reaction	5
was associated with	5
the causative agent	5
of s protein	5
similar to those	5
properties of the	5
of pdcov replication	5
a comparison of	5
confirmed that the	5
of the coronaviridae	5
the full genome	5
secondary structure of	5
sialic acid binding	5
calculated for each	5
only a few	5
does not seem	5
of the full	5
clinical signs of	5
have been used	5
and can be	5
for the florida	5
the ga cwl	5
were constructed using	5
causative agent of	5
s gene sequence	5
has been reported	5
two of the	5
to type i	5
in the overlap	5
sequences from the	5
s gene of	5
followed by the	5
the alignment of	5
of acute infectious	5
shrnas targeting the	5
lamp and rt	5
primers p and	5
viruses with a	5
downstream of sh	5
of the most	5
linked glycosylation sites	5
phylogenetic analyses of	5
of genetically engineered	5
in this case	5
a type i	5
diarrhoea in dogs	5
epidemic diarrhea viruses	5
from the h	5
none of the	5
h and h	5
s protein is	5
s and e	5
strains lsu and	5
in the pathogenic	5
in h n	5
viruses have been	5
of the frameshift	5
by agarose gel	5
amino acid deletions	5
and is a	5
the amber codon	5
detection of the	5
of each of	5
and compared with	5
showed the highest	4
deduced amino acids	4
indel pedv strains	4
gene coding for	4
was found between	4
of the samples	4
influenza virus in	4
one piglet was	4
kb in size	4
fecal samples from	4
by the manufacturer	4
the nucleoprotein mrna	4
t b e	4
s glycoprotein is	4
collected from the	4
strains have been	4
s gene was	4
protein genes of	4
in amino acid	4
to target the	4
potential glycosylation sites	4
with each other	4
of retroviral rnas	4
serves as a	4
the f region	4
the genome and	4
resulting in the	4
baculovirus expression system	4
strand cdna synthesis	4
of positive samples	4
a agarose gel	4
the optimal reaction	4
the infected cells	4
citrus tristeza virus	4
genetic characterization of	4
was provided by	4
in the pb	4
no amino acid	4
viral diarrhea virus	4
resulting in a	4
seven of the	4
of pedv infection	4
the addition of	4
based on this	4
and litter extracts	4
order to identify	4
conserved among all	4
the polymerase gene	4
usage variation among	4
and sequencing of	4
the titer of	4
the phylogenetic analysis	4
as part of	4
two open reading	4
can be seen	4
the need for	4
genbank accession no	4
were cultured in	4
a phylogenetic tree	4
n genes of	4
bursal disease virus	4
our results indicate	4
coronavirus leader rna	4
in vitro transcription	4
stranded rna virus	4
amino acid difference	4
were designed to	4
with diarrhea in	4
of the new	4
shows that the	4
were aligned and	4
m t a	4
the sour orange	4
the range of	4
and the severity	4
other cdv strains	4
shown to have	4
on an abi	4
axis values in	4
that the amount	4
of novel picornaviruses	4
the accession numbers	4
transmissible gastroenteritis coronavirus	4
bat fecal samples	4
plays important roles	4
on the phylogenetic	4
be separated into	4
the orf gene	4
sour orange plants	4
dna fragments were	4
not been reported	4
diagnosis of pedv	4
and infected with	4
virus at positions	4
each other than	4
in domestic pigs	4
is divided into	4
was suggested that	4
of the corresponding	4
a and cpv	4
were aligned with	4
for avian influenza	4
fusion of the	4
of neutralizing antibodies	4
serially diluted in	4
of min at	4
of viral proteins	4
is believed to	4
been isolated from	4
is the major	4
h n infections	4
from a single	4
serotypes of ibv	4
during human infection	4
bat species in	4
to be determined	4
protein of the	4
reverse transcription loop	4
the sequence analysis	4
important roles in	4
of nucleotides in	4
suggested that the	4
in the other	4
are thought to	4
the function of	4
there were no	4
in a total	4
domain of the	4
kinase ii phosphorylation	4
the complete d	4
florida and connecticut	4
there is no	4
nature of the	4
in this article	4
were determined by	4
to monitor the	4
piece of lung	4
a group of	4
phylogenetic analysis based	4
the fipv orf	4
the florida strain	4
coli dh a	4
differences between pathogenic	4
on the genome	4
to increase the	4
the main factor	4
site for the	4
of s gene	4
days post infection	4
is most closely	4
was found at	4
are very similar	4
hemagglutinating encephalomyelitis virus	4
cloned and sequenced	4
the h and	4
the s a	4
the genome organization	4
in the predicted	4
phylogenetic analysis and	4
pcr was performed	4
the infected piglets	4
mutational pressure is	4
the porcine transmissible	4
restriction fragment length	4
ga cwl virus	4
ppi t t	4
of the stop	4
and segment b	4
analysis of these	4
sequence was used	4
mrna cap methylation	4
study was supported	4
and connecticut strains	4
the other hand	4
high number of	4
of the hairpin	4
for puuv rna	4
e i c	4
have been shown	4
similarity between the	4
methyl transferase assay	4
to the high	4
a laboratory manual	4
sense and antisense	4
are located within	4
of orf b	4
the immune response	4
e a a	4
number of differences	4
the rdrp and	4
the n and	4
acid sequence data	4
sequence of orf	4
chest cavity lavage	4
can be found	4
e i t	4
a virus type	4
viral and cellular	4
the reaction mixture	4
variation in synonymous	4
limit of the	4
of the turkey	4
fact that the	4
n gene has	4
cycle sequencing kit	4
first and second	4
of synonymous codon	4
detected in bats	4
target the m	4
the high case	4
were downloaded from	4
accordance with the	4
the closely related	4
was shown to	4
are present in	4
ribosomal frame shift	4
sequences of s	4
the pcr product	4
k c b	4
the virus may	4
amplified dna products	4
amino acids were	4
loss of the	4
entire genome of	4
in synonymous codon	4
the frequency of	4
the negative control	4
of the sy	4
proportion of gc	4
of turkey hepatitis	4
to characterize the	4
of the sars	4
compared to other	4
is one of	4
strains had the	4
other pedv strains	4
extent of codon	4
of the pcr	4
the polymerase complex	4
detected from the	4
inhibition of pdcov	4
the kda protein	4
c sei t	4
neurological disease in	4
a g m	4
a sei c	4
compared to those	4
mutation rate of	4
and the h	4
was obtained from	4
were submitted to	4
compared with that	4
newly isolated viruses	4
replication in vitro	4
from chicken and	4
was performed in	4
the specificity of	4
interstructural gene region	4
in the swine	4
n viruses isolated	4
inhibition of tgev	4
we investigated the	4
revealed the presence	4
we report the	4
disease in cattle	4
in the third	4
collected from chickens	4
further studies are	4
from the other	4
high specificity and	4
t ppi a	4
was collected from	4
that they are	4
vt and mor	4
cells were inoculated	4
ability to infect	4
large s gene	4
h n in	4
were incubated at	4
from sequence analysis	4
are grateful to	4
derived from a	4
established or putative	4
were generated with	4
of s subunit	4
consequence of the	4
all cdv strains	4
g r a	4
the third position	4
the spike protein	4
development of genetically	4
used to differentiate	4
strains in the	4
in the cytoplasm	4
a nested set	4
of infection and	4
and a high	4
molecular evolution of	4
change in the	4
protein is the	4
genetic differences between	4
was developed for	4
methyl transferase domain	4
have nt differences	4
infected with pdcov	4
virulence of h	4
were associated with	4
sequence and phylogenetic	4
in the following	4
the levels of	4
to be investigated	4
of pastv in	4
sensitivity and specificity	4
differ in their	4
are not essential	4
the virus titer	4
due to a	4
also found to	4
genome was sequenced	4
to differentiate the	4
from each other	4
and the m	4
and have been	4
to the poly	4
of the polymerase	4
site of the	4
been infected with	4
according to their	4
comparison with the	4
the ibv strains	4
could affect the	4
fragment length polymorphism	4
tested for the	4
the structural genes	4
the predicted amino	4
the antigenic properties	4
sequences were compared	4
complete d pol	4
used to amplify	4
cells were transfected	4
on the basis	4
with cell culture	4
have been infected	4
rna was used	4
the florida and	4
the mutated strain	4
in the family	4
base usage was	4
were incubated for	4
they did not	4
pressure is the	4
piglets were inoculated	4
ml dmem fcs	4
aligned using the	4
of the p	4
for the rna	4
and ebcv strains	4
the respiratory tract	4
t m t	4
isolates from china	4
in the trachea	4
occurs in the	4
are involved in	4
the induction of	4
from chronically infected	4
with the highest	4
cleavage site of	4
the faeces of	4
can be separated	4
and human coronavirus	4
the origin of	4
were separated by	4
indicate that the	4
chickens with rss	4
sequencing reactions were	4
it might be	4
for detecting pedv	4
the rest of	4
of the ctv	4
for the h	4
c t m	4
values in coa	4
of the sequence	4
turkey hepatitis viruses	4
was determined using	4
a common ancestor	4
dmem fcs ps	4
provided by the	4
the ibdv genome	4
lower than the	4
the pb gene	4
of n gene	4
from the feces	4
nested set of	4
the viral mrna	4
is a type	4
of the target	4
for efficient frameshifting	4
sequence at the	4
analysis based on	4
in the virus	4
ii phosphorylation sites	4
fecal samples of	4
central european puuv	4
subunit of the	4
with a predicted	4
a detection limit	4
in the coding	4
of the fipv	4
a consequence of	4
third codon position	4
pcr amplification was	4
with high specificity	4
the connecticut and	4
the overlap region	4
was isolated in	4
e a g	4
viruses such as	4
human adaptive markers	4
differences are observed	4
i fcov and	4
not show any	4
supported by a	4
were cloned into	4
a reverse transcription	4
fetal bovine serum	4
exception of the	4
a chicken egypt	4
an abi prism	4
rbcv strains lsu	4
genes from the	4
for virus replication	4
gc content and	4
the intestinal contents	4
sensitive than the	4
g m t	4
contribute to the	4
rna sequences from	4
samples were from	4
of the shifty	4
rich in the	4
complementary to a	4
been shown that	4
differences observed between	4
t t sei	4
and is the	4
determined by the	4
transfected with pgenesil	4
a single stranded	4
for the first	4
we did not	4
amino acids with	4
on the deduced	4
of the nucleocapsid	4
in the original	4
of its genome	4
causative agents of	4
detection of caliciviruses	4
were as follows	4
from the faeces	4
of porcine transmissible	4
for in vitro	4
identity with the	4
large t antigen	4
were different from	4
amino acid mutations	4
of the consensus	4
and for the	4
genome sequencing of	4
within the same	4
stranded rna genome	4
been deposited in	4
is present in	4
ll and l	4
of viral rna	4
are maintained in	4
conserved amino acid	4
g a m	4
believed to be	4
the inhibition of	4
been associated with	4
identification of a	4
tgev infection in	4
demonstrated that the	4
are encoded by	4
few amino acid	4
of the f	4
as described earlier	4
are associated with	4
due to their	4
nucleotide sequence identity	4
associated with human	4
among different strains	4
a highly conserved	4
of bank voles	4
antigenic properties of	4
study was to	4
an important factor	4
sequences of other	4
m k t	4
the inability of	4
puuv strain from	4
to the family	4
of puumala hantavirus	4
coronaviruses have been	4
in the viral	4
subgenomic mrna species	4
and a final	4
the p region	4
the ratio of	4
n highly pathogenic	4
the attenuated viruses	4
did not include	4
m protein is	4
primer pair f	4
cell culture and	4
viruses have nt	4
in different organisms	4
genes of the	4
a novel adenovirus	4
i t t	4
of complete genome	4
rnas in the	4
detection of porcine	4
the replicase proteins	4
d pol sequences	4
in the early	4
parts of the	4
in bat populations	4
virus detected in	4
collected from a	4
of the mhv	4
compared with other	4
of the chinju	4
in southern china	4
the identification of	4
a and a	4
is possible that	4
other retroviral rnas	4
the amplified dna	4
the reaction was	4
did not reveal	4
acute haemorrhagic enteritis	4
it would be	4
has been a	4
of a h	4
information about the	4
a protein of	4
in the entire	4
to have an	4
the emergence of	4
bovine viral diarrhea	4
were compared to	4
orf ab and	4
transmission to other	4
when compared with	4
of the world	4
in chicken and	4
and p proteins	4
of a number	4
the current study	4
the middle east	4
but not in	4
different regions of	4
available in genbank	4
between virulent and	4
it is likely	4
performed with the	4
used to design	4
within a serotype	4
molecular epidemiology and	4
h n highly	4
were incubated with	4
pedv s protein	4
u uua aac	4
has been proposed	4
s gene deletion	4
viruses as well	4
target for the	4
were generated using	4
nucleotides of the	4
in the host	4
in the secondary	4
have been deposited	4
phylogenetic relationships between	4
the original clinical	4
g s e	4
were extracted from	4
of type i	4
were tested for	4
the whole genome	4
by the virus	4
to the genus	4
m c g	4
of caliciviruses in	4
to assess the	4
of pedv was	4
similarity of the	4
codon usage for	4
a close relationship	4
the cdna fragments	4
in the gut	4
be seen from	4
of sm gene	4
were collected in	4
those of cv	4
organization of the	4
cells were seeded	4
were shown to	4
associated with viral	4
the fujian samples	4
and avian infectious	4
it was suggested	4
information for the	4
this suggests that	4
evidence that the	4
sequence in the	4
line with the	4
tree based on	4
accession numbers of	4
the frequencies of	4
purdue strain of	4
variation in codon	4
virus infection in	4
is more sensitive	4
birds were given	4
codon in the	4
as a result	4
h n cases	4
close to the	4
s glycoprotein of	4
could be due	4
used to detect	4
it consisted of	4
virus with a	4
amplification and sequencing	4
was more closely	4
addition to the	4
targeted the m	3
number of codons	3
acute enteritis and	3
acid residues in	3
function of these	3
synthesis of a	3
the feces were	3
and used to	3
conserved in the	3
pedvs isolated from	3
between different strains	3
and translational selection	3
lamp is more	3
lm of each	3
complete genomic sequences	3
be predicted that	3
a and jhm	3
of cl and	3
polyproteins a and	3
these sequences are	3
type i virus	3
cell culture supernatant	3
of tcid ml	3
terminal portion of	3
of the pb	3
third of the	3
highly virulent strains	3
viruses closer to	3
at c and	3
in cl and	3
for use in	3
failure of the	3
may affect the	3
of the national	3
in the samples	3
in our previous	3
evolution of type	3
were of the	3
for the production	3
adapted to the	3
determined by quantitative	3
and ok were	3
science and technology	3
did not have	3
performed in a	3
and in vivo	3
in the current	3
range of hosts	3
to the group	3
half of the	3
control and prevention	3
for rna extraction	3
using the method	3
influenced by the	3
n gene open	3
were determined and	3
thv and thv	3
at the synonymous	3
at a concentration	3
to investigate if	3
is complementary to	3
found in all	3
is located at	3
the open reading	3
was different from	3
and used for	3
synthesis of the	3
corresponding sequences of	3
t and sp	3
the cell culture	3
porcine kidney cells	3
determine whether the	3
m gpppa m	3
acid substitutions within	3
a new genus	3
the c gene	3
rna sequence was	3
the viral rnas	3
between the connecticut	3
mortality up to	3
in the new	3
genetic antigenic drift	3
of sequence data	3
porcine pseudorabies virus	3
n proteins of	3
in comparison to	3
and the nucleotide	3
associated with attenuation	3
among the asia	3
were excised from	3
with the host	3
is the high	3
an indication of	3
analysis of a	3
h gene of	3
substitutions within the	3
are observed between	3
the protease domain	3
infection with other	3
in this region	3
that the leader	3
sequences used to	3
by site directed	3
viral genome was	3
might affect codon	3
of the amber	3
were detected in	3
and the host	3
adjacent to the	3
the same sample	3
also related to	3
collected from turkey	3
the fraction of	3
as long as	3
most abundant component	3
rnai has been	3
analysis of cdv	3
sei t ppi	3
in the signal	3
proteolytic cleavage site	3
l protein in	3
at days post	3
for the rapid	3
u uua aau	3
the conventional rt	3
the dna polymerase	3
the viral genomes	3
codon positions was	3
of the epidemic	3
the fipv genome	3
changes associated with	3
this type of	3
since the first	3
of bovine kobuvirus	3
form of the	3
reference to the	3
recent studies have	3
structural proteins of	3
that pedv is	3
the g pedvs	3
and canine coronavirus	3
to distinguish between	3
still causes many	3
products were electrophoresed	3
was not found	3
influenza h n	3
found at the	3
serial dilutions of	3
and i orf	3
terminus of the	3
of a new	3
the intensity of	3
the most variable	3
from veroe cells	3
required for the	3
a correlation between	3
and the genomic	3
be the most	3
could be used	3
in the respiratory	3
in situ hybridization	3
is a highly	3
genes of pedv	3
virus is a	3
attenuation of the	3
complete cds of	3
to prevent the	3
tibetan mastiff that	3
a new sars	3
isolation and characterization	3
site is specific	3
gene and the	3
specimens by rt	3
of genetic diversity	3
acid differences among	3
s and he	3
cdna synthesis kit	3
sequence changes in	3
encoded a protein	3
for each species	3
adaptation of the	3
study of the	3
the control of	3
carried out by	3
to develop a	3
pathogenic h n	3
nucleotides in the	3
infected with tgev	3
for the utr	3
pigs of all	3
the small membrane	3
extracted rna was	3
proposed to be	3
be useful in	3
usage variation in	3
from bank voles	3
bank vole kidney	3
will be interesting	3
had been shown	3
diluted in pbs	3
and ch gsjiii	3
is important to	3
we have cloned	3
of gc content	3
is likely that	3
resuspended in ml	3
host immune response	3
polyprotein and segment	3
have the same	3
and those of	3
causes an acute	3
isolates originated from	3
of viral mrna	3
electrophoresis in agarose	3
sequence encoding the	3
was taken for	3
with the h	3
chinese field strains	3
coding sequences were	3
pcr was conducted	3
and vp genes	3
regardless of the	3
green monkey kidney	3
based on partial	3
based differential rrt	3
genome of a	3
of cpg and	3
was performed to	3
group i coronaviruses	3
of the sh	3
in viral mrna	3
differences amino acids	3
of the ark	3
in the pedv	3
a tail was	3
in the corresponding	3
of the differences	3
is identical to	3
infected with vt	3
primers that recognize	3
to the genbank	3
will provide a	3
s deduced amino	3
nucleotide and predicted	3
overlap of the	3
virus infections in	3
hairpin rnas targeting	3
strains isolated from	3
all the amino	3
homology of the	3
was used in	3
calculated by the	3
recombination event between	3
between amino acids	3
of pedv based	3
in some retroviral	3
observed for the	3
of their genomes	3
adenoviruses in bats	3
of emerging viruses	3
by quantitative real	3
cc strain was	3
m c t	3
s gene and	3
was complementary to	3
positive samples for	3
fell into two	3
on codon usage	3
a pseudoknotted structure	3
which was different	3
more conserved to	3
orf of pedv	3
park et al	3
cloning and sequence	3
the b orf	3
would predict that	3
guanine methyl transferase	3
studies on the	3
that account for	3
glycoprotein for the	3
there was a	3
the recombinant plasmids	3
the ark ark	3
length of the	3
and dissolved in	3
in the first	3
at rich in	3
relationship of the	3
cdna libraries were	3
of the actual	3
glycosylation site upstream	3
time quantitative rt	3
and the reaction	3
of normal chicken	3
and placed in	3
a potential pseudoknot	3
the age of	3
titer of tcid	3
of gfcov fr	3
the conservation of	3
been identified in	3
usage bias and	3
the cv and	3
from other strains	3
virus neutralization assay	3
in sequence to	3
of ibv in	3
dna and rna	3
kb in length	3
are identical in	3
two antigenic groups	3
t t ppi	3
that have been	3
a set of	3
amino acid change	3
the nucleocapsid gene	3
four structural proteins	3
without prior amplification	3
the qpcr analysis	3
human infections with	3
cattle brain tissue	3
be able to	3
used as the	3
amino acids and	3
dependent protein kinase	3
codon bias in	3
strains were clustered	3
coronaviruses belonging to	3
fipv orf a	3
infect a wide	3
insight into the	3
that although the	3
fragments of the	3
position in the	3
in a ll	3
studies indicate that	3
infected alemow plants	3
strains and the	3
sequenced in both	3
when the cells	3
and the relative	3
a gene of	3
from the genbank	3
with the genome	3
the complete nucleotide	3
type ii feline	3
dna polymerase gene	3
suggesting the presence	3
pathogenicity of the	3
is part of	3
present on the	3
c a j	3
were compared with	3
primers based on	3
p complex from	3
the same genes	3
than that observed	3
ranged from to	3
the titers of	3
play a key	3
identical to the	3
vitro methyl transferase	3
higher than that	3
potential protein kinase	3
n viruses are	3
in the codon	3
replication of pdcov	3
correspond to the	3
attenuated form of	3
morbidity and mortality	3
transcriptional modification of	3
and maintenance of	3
and the ga	3
a series of	3
of the pathogenicity	3
percentage of positive	3
was observed between	3
were the same	3
aa change of	3
the s genes	3
and of the	3
during replication in	3
nsp is a	3
pressure and translational	3
stop codon in	3
prompted us to	3
human coronavirus e	3
reported in the	3
gel extraction kit	3
was conducted with	3
lamp for pedv	3
over year old	3
the s segment	3
reaction volume using	3
first strand cdna	3
in neonatal piglets	3
protein for the	3
analysis was carried	3
had a close	3
sequences of tgev	3
fipv interstructural gene	3
identical to that	3
changes in nsp	3
sites of the	3
variation in the	3
the disease have	3
determined and compared	3
large number of	3
recommended by the	3
of ibv and	3
t a ppi	3
a c m	3
veroe cells and	3
piglets infected experimentally	3
kinase phosphorylation site	3
of cytopathic effects	3
frequency of the	3
in ml of	3
a a sei	3
placed genus viruses	3
primer set for	3
the macrogen institute	3
structural proteins vp	3
the molecular epidemiology	3
in bats of	3
on highly pathogenic	3
ten amino acid	3
not interfere with	3
role in virus	3
of the wells	3
allantoic fluid of	3
the selection of	3
the phylogenetic relationships	3
korean bovine kobuvirus	3
to other species	3
using the clustal	3
astrovirus infection in	3
likely that the	3
sequence was present	3
cdna for the	3
and porcine respiratory	3
in orf a	3
presence of several	3
in recent years	3
high prevalence of	3
bases upstream of	3
determinant of host	3
the genus mamastrovirus	3
pig fecal samples	3
taiwan tc ay	3
in the infected	3
the accurate and	3
to be highly	3
xi dna sequencer	3
was reported that	3
the wells were	3
the interference efficiency	3
by a final	3
with other viruses	3
inhibition of porcine	3
f protein is	3
viruses belonging to	3
infection and the	3
to the s	3
isolates are closely	3
have been determined	3
this is consistent	3
which was previously	3
reported in this	3
that the n	3
fragment of the	3
to play a	3
in england in	3
ntrs of chk	3
associated with reduced	3
s subunit is	3
virulence and human	3
the genetic diversity	3
amino acid variations	3
than to the	3
originated from a	3
there are other	3
multiple sequence alignment	3
nt for the	3
phylogenetic analysis was	3
gene region is	3
of gene expression	3
the reaction mix	3
pcr rflp serotype	3
a stable hairpin	3
similarity to the	3
between the ga	3
tgev rna polymerase	3
the following accession	3
viral rnas in	3
sequence data were	3
expressed in the	3
a range of	3
genes of porcine	3
cap methyl transferase	3
incubated for h	3
downloaded from genbank	3
at base positions	3
the same virus	3
in poultry and	3
disease in chickens	3
genbank under accession	3
were more conserved	3
these results suggest	3
n protein has	3
development of vaccines	3
embryonated chicken eggs	3
other than to	3
bovine kobuvirus was	3
is related to	3
and phylogenetic trees	3
human and animal	3
known to be	3
unmethylated cpg motifs	3
cells and the	3
primers targeting the	3
feces using trizol	3
of the eight	3
from a tibetan	3
substitutions within s	3
were grown in	3
that the codon	3
and host range	3
large spike gene	3
identical in all	3
h n isolates	3
ends of the	3
pedv from clinical	3
that cause diarrhea	3
large deletion in	3
ethidium bromide staining	3
of human and	3
structure downstream of	3
to determine if	3
the stop codon	3
korean kobuvirus strains	3
with the ability	3
have evolved from	3
of each virus	3
coaxial stacking of	3
acids with predicted	3
is conserved in	3
inspection of the	3
is an enveloped	3
was added and	3
in piglets infected	3
this does not	3
nucleotide sequence encoding	3
number of other	3
acid differences are	3
in the gag	3
to a previous	3
from a common	3
two pairs of	3
transfected with shrna	3
the characterizations of	3
indonesian h n	3
differences between virulent	3
which were then	3
and antisense primer	3
connecting loops ll	3
insights into understanding	3
viral replication in	3
analysis of genetic	3
ibv strains was	3
conserved region of	3
evaluate risks for	3
sequences at the	3
respiratory syndrome coronavirus	3
protective immune response	3
of the fecal	3
relationship between the	3
changes in spike	3
changes resulting in	3
by a short	3
value in coa	3
are located at	3
a tibetan mastiff	3
humans in indonesia	3
murine hepatitis virus	3
submitted to the	3
to form a	3
indicates that the	3
that it is	3
predicted mr of	3
just downstream of	3
the early region	3
recombinant plasmids were	3
as for the	3
to the corresponding	3
ibv gray and	3
the ibv gray	3
variable orf in	3
recovered from cells	3
inability of the	3
report on the	3
isolated from humans	3
was also detected	3
s gene nucleotide	3
normal chicken embryos	3
at the macrogen	3
south korea from	3
to evaluate the	3
set for the	3
that the replicase	3
here we report	3
orf a of	3
we wanted to	3
m r of	3
lamp assay with	3
trees infected with	3
fatality rate of	3
total reaction volume	3
were present at	3
determined to be	3
a pair of	3
transcription and replication	3
in the loop	3
figure shows that	3
polymerase gene of	3
from genbank and	3
is a recently	3
difference in the	3
the target dna	3
that may be	3
high frequency of	3
regulation of viral	3
the p protein	3
cells were harvested	3
coding region of	3
of rinderpest virus	3
were washed with	3
occurrence of stop	3
domain iii alone	3
of s and	3
cdna fragments were	3
significant difference between	3
one viral strain	3
stretch of nucleotides	3
were highly conserved	3
and vaccine strains	3
was inoculated with	3
found upstream of	3
human and avian	3
rna secondary structure	3
two species of	3
rdrp and orf	3
sequenced and compared	3
some retroviral rnas	3
infections in humans	3
in the range	3
the general correlation	3
the route of	3
to replicate in	3
usage at each	3
of the nucleotides	3
the first time	3
differentiate the two	3
reactions were performed	3
the reaction conditions	3
rapid detection of	3
the basis for	3
of poultry outbreaks	3
classical methylation pathway	3
first detection of	3
shrna recombinant plasmids	3
this resulted in	3
maintained in bat	3
p mutations were	3
in the rna	3
disease have been	3
s and m	3
have not been	3
and other members	3
in vitro and	3
subunit for the	3
the results from	3
concentration of lg	3
region between the	3
to a lesser	3
pedv is most	3
the proteolytic cleavage	3
of a virus	3
at position for	3
a single orf	3
ay taiwan tc	3
site upstream the	3
sequences upstream of	3
immediately adjacent to	3
variations in the	3
and it is	3
plays a role	3
glycoprotein genes of	3
evolution of h	3
at p and	3
of dinucleotides in	3
the n proteins	3
in hubei province	3
the shifty sequence	3
acid binding activity	3
the evolutionary relationships	3
viral rna in	3
the regulation of	3
glycoprotein of the	3
is dispensable for	3
antigenic variation of	3